Publicações

@article{Matsumura2019,

title = {Rescue of Citrus sudden death-associated virus in Nicotiana benthamiana plants from cloned cDNA: insights into mechanisms of expression of the three capsid proteins},

author = {Emilyn E. Matsumura and Helvécio D. Coletta-Filho and Marcos A. Machado and Shahideh Nouri and Bryce W. Falk},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12780 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12780 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12780},

doi = {10.1111/MPP.12780},

issn = {1364-3703},

year  = {2019},

date = {2019-01-01},

journal = {Molecular Plant Pathology},

volume = {20},

issue = {5},

pages = {611-625},

publisher = {John Wiley & Sons, Ltd},

abstract = {Citrus sudden death-associated virus (CSDaV) is a member of the genus Marafivirus in the family Tymoviridae, and has been associated with citrus sudden death (CSD) disease in Brazil. Difficulties in the purification of CSDaV from infected citrus plants have prevented progress in the investigation of the role of this virus in CSD and an understanding of its molecular biology. In this work, we have constructed a full-length cDNA clone of CSDaV driven by the 35S promoter (35SRbz-CSDaV). Agrobacterium tumefaciens-mediated inoculation of 35SRbz-CSDaV in Nicotiana benthamiana plants enabled a fast recovery of large amounts of virions from the agroinfiltrated leaves, which allowed a better molecular characterization of CSDaV. In vivo analyses of mutant versions of 35SRbz-CSDaV revealed the expression strategies used by CSDaV for production of the capsid proteins (CPs). We showed that CSDaV virions contain three forms of CP, each of which is generated from the same coding sequence, but by different mechanisms. The major CPp21 is a product of direct translation by leaky scanning from the second start codon in the subgenomic RNA (sgRNA), whereas the minor CPs, p25 and p23, are produced by direct translation from the first start codon in the sgRNA and by trans-proteolytic cleavage processing derived from the p25 precursor, respectively. Together, these findings contribute to advance our understanding of CSDaV genome expression strategies. In addition, the construction and characterization of the CSDaV infectious clone represent important steps towards the investigation of the role of this virus in CSD and of its use as a tool for citrus biotechnology.},

keywords = {associated virus, capsid protein expression, Citrus disease, citrus sudden death, infectious clone, Marafivirus, Tymoviridae},

pubstate = {published},

tppubtype = {article}

}

@article{Fadel2018,

title = {Citrus sudden death-associated virus (CSDaV) and citrus tristeza virus (CTV) in eleven rootstocks for ‘Valência’ sweet orange},

author = {André Luiz Fadel and Francisco Assis Alves Mourão Filho and Eduardo Sanches Stuchi and Nelson Arno Wulff and Hilton Thadeu Zarate Couto},

url = {http://www.scielo.br/j/rbf/a/353wfytmSS9p6tP4ZhXdLWD/?lang=en},

doi = {10.1590/0100-29452018788},

issn = {0100-2945},

year  = {2018},

date = {2018-01-01},

journal = {Revista Brasileira de Fruticultura},

volume = {40},

issue = {4},

pages = {774-778},

publisher = {Sociedade Brasileira de Fruticultura},

abstract = {Abstract Citrus sudden death (CSD) is a highly destructive disease and has caused the eradication of millions of trees in southern Brazil within the last 15 years. In spite of the exact cause of CSD has not been determined, evidences have shown that this disease can be transmitted by biotic vectors. Disease incidence in sweet orange scions is related to the rootstock, and the combination with ‘Rangpur’ lime is the most affected. On the other hand, there are evidences of a relation between CSD affected trees and the presence of the Citrus sudden death associated virus (CSDaV) and/or Citrus tristeza virus (CTV). Based on such information, this study has been carried out to determine the presence of CSDaV and CTV, and the association between each other in eleven rootstocks for ‘Valencia’ sweet orange. The results presented herein showed differences related to the presence of CSDaV and CTV in different rootstocks for ‘Valencia’ sweet orange and no relation between the presence of CSDaV and CTV.},

keywords = {Marafivirus, Poncirus trifoliata, symptoms, transmission},

pubstate = {published},

tppubtype = {article}

}