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2021
Müller, Cristiane; Esteves, Mariana Bossi; Kleina, Heloisa Thomazi; Nondillo, Aline; Botton, Marcos; Lopes, João Roberto Spotti
First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil Journal Article
Em: Tropical Plant Pathology, vol. 46, iss. 3, pp. 386-391, 2021, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Cicadellinae, Plum leaf scald, transmission, Vector-borne bacterium
@article{nokey,
title = {First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil},
author = {Cristiane Müller and Mariana Bossi Esteves and Heloisa Thomazi Kleina and Aline Nondillo and Marcos Botton and João Roberto Spotti Lopes},
url = {https://link.springer.com/article/10.1007/s40858-021-00430-8},
doi = {10.1007/S40858-021-00430-8/METRICS},
issn = {19832052},
year = {2021},
date = {2021-01-01},
journal = {Tropical Plant Pathology},
volume = {46},
issue = {3},
pages = {386-391},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Plum leaf scald (PLS), caused by the vector-borne bacterium Xylella fastidiosa, is a major obstacle to the expansion of plum crops in Brazil. In other affected crops, this pathogen is naturally transmitted mainly by sharpshooter leafhoppers (Hemiptera: Cicadellidae: Cicadellinae). Despite the importance of PLS, the vector species responsible for bacterial spread in plums remain unknown. This study, therefore, aimed to determine the X. fastidiosa transmission ability of three sharpshooter species commonly found in plum orchards in southern and southeastern Brazil: Macugonalia cavifrons (Stål), Macugonalia leucomelas (Walker), and Sibovia sagata (Signoret). After a 72-h acquisition access period to plum trees infected with X. fastidiosa subsp. multiplex (sequence type 67), the insects were transferred for an inoculation access period of 96 h to 10–20 healthy Prunus salicina plants for each sharpshooter species, using four insects per plant. Bacterial infection in the plants was verified 7 months after inoculation by polymerase chain reaction. Results showed that the three sharpshooter species were able to transmit X. fastidiosa to plums, with mean transmission efficiencies by single insects of 16%, 14%, and 18% for M. cavifrons, M. leucomelas, and S. sagata, respectively. To our knowledge, this study is the first to identify vector species and prove the transmission of a PLS strain of X. fastidiosa subsp. multiplex by sharpshooters in Brazil.},
keywords = {Cicadellinae, Plum leaf scald, transmission, Vector-borne bacterium},
pubstate = {published},
tppubtype = {article}
}
Müller, Cristiane; Esteves, Mariana Bossi; Kleina, Heloisa Thomazi; Nondillo, Aline; Botton, Marcos; Lopes, João Roberto Spotti
First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil Journal Article
Em: Tropical Plant Pathology, vol. 46, iss. 3, pp. 386-391, 2021, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Cicadellinae, Plum leaf scald, transmission, Vector-borne bacterium
@article{nokey,
title = {First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil},
author = {Cristiane Müller and Mariana Bossi Esteves and Heloisa Thomazi Kleina and Aline Nondillo and Marcos Botton and João Roberto Spotti Lopes},
url = {https://link.springer.com/article/10.1007/s40858-021-00430-8},
doi = {10.1007/S40858-021-00430-8/METRICS},
issn = {19832052},
year = {2021},
date = {2021-01-01},
journal = {Tropical Plant Pathology},
volume = {46},
issue = {3},
pages = {386-391},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Plum leaf scald (PLS), caused by the vector-borne bacterium Xylella fastidiosa, is a major obstacle to the expansion of plum crops in Brazil. In other affected crops, this pathogen is naturally transmitted mainly by sharpshooter leafhoppers (Hemiptera: Cicadellidae: Cicadellinae). Despite the importance of PLS, the vector species responsible for bacterial spread in plums remain unknown. This study, therefore, aimed to determine the X. fastidiosa transmission ability of three sharpshooter species commonly found in plum orchards in southern and southeastern Brazil: Macugonalia cavifrons (Stål), Macugonalia leucomelas (Walker), and Sibovia sagata (Signoret). After a 72-h acquisition access period to plum trees infected with X. fastidiosa subsp. multiplex (sequence type 67), the insects were transferred for an inoculation access period of 96 h to 10–20 healthy Prunus salicina plants for each sharpshooter species, using four insects per plant. Bacterial infection in the plants was verified 7 months after inoculation by polymerase chain reaction. Results showed that the three sharpshooter species were able to transmit X. fastidiosa to plums, with mean transmission efficiencies by single insects of 16%, 14%, and 18% for M. cavifrons, M. leucomelas, and S. sagata, respectively. To our knowledge, this study is the first to identify vector species and prove the transmission of a PLS strain of X. fastidiosa subsp. multiplex by sharpshooters in Brazil.},
keywords = {Cicadellinae, Plum leaf scald, transmission, Vector-borne bacterium},
pubstate = {published},
tppubtype = {article}
}
2018
Fadel, André Luiz; Filho, Francisco Assis Alves Mourão; Stuchi, Eduardo Sanches; Wulff, Nelson Arno; Couto, Hilton Thadeu Zarate
Citrus sudden death-associated virus (CSDaV) and citrus tristeza virus (CTV) in eleven rootstocks for ‘Valência’ sweet orange Journal Article
Em: Revista Brasileira de Fruticultura, vol. 40, iss. 4, pp. 774-778, 2018, ISSN: 0100-2945.
Resumo | Links | BibTeX | Tags: Marafivirus, Poncirus trifoliata, symptoms, transmission
@article{Fadel2018,
title = {Citrus sudden death-associated virus (CSDaV) and citrus tristeza virus (CTV) in eleven rootstocks for ‘Valência’ sweet orange},
author = {André Luiz Fadel and Francisco Assis Alves Mourão Filho and Eduardo Sanches Stuchi and Nelson Arno Wulff and Hilton Thadeu Zarate Couto},
url = {http://www.scielo.br/j/rbf/a/353wfytmSS9p6tP4ZhXdLWD/?lang=en},
doi = {10.1590/0100-29452018788},
issn = {0100-2945},
year = {2018},
date = {2018-01-01},
journal = {Revista Brasileira de Fruticultura},
volume = {40},
issue = {4},
pages = {774-778},
publisher = {Sociedade Brasileira de Fruticultura},
abstract = {Abstract Citrus sudden death (CSD) is a highly destructive disease and has caused the eradication of millions of trees in southern Brazil within the last 15 years. In spite of the exact cause of CSD has not been determined, evidences have shown that this disease can be transmitted by biotic vectors. Disease incidence in sweet orange scions is related to the rootstock, and the combination with ‘Rangpur’ lime is the most affected. On the other hand, there are evidences of a relation between CSD affected trees and the presence of the Citrus sudden death associated virus (CSDaV) and/or Citrus tristeza virus (CTV). Based on such information, this study has been carried out to determine the presence of CSDaV and CTV, and the association between each other in eleven rootstocks for ‘Valencia’ sweet orange. The results presented herein showed differences related to the presence of CSDaV and CTV in different rootstocks for ‘Valencia’ sweet orange and no relation between the presence of CSDaV and CTV.},
keywords = {Marafivirus, Poncirus trifoliata, symptoms, transmission},
pubstate = {published},
tppubtype = {article}
}