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2022
Müller, Cristiane; Esteves, Mariana Bossi; Kleina, Heloisa Thomazi; Sales, Tiago Melo; Liva, Karina Botini; Balbinote, Juliana; Lopes, João Roberto Spotti
Weeds as alternative hosts of Xylella fastidiosa in Brazilian plum orchards Journal Article
Em: Journal of Plant Pathology, vol. 104, iss. 2, pp. 487-493, 2022, ISSN: 22397264.
Resumo | Links | BibTeX | Tags: Plum leaf scald; Dissemination, Prunus salicina, Sources of inoculum
@article{nokey,
title = {Weeds as alternative hosts of Xylella fastidiosa in Brazilian plum orchards},
author = {Cristiane Müller and Mariana Bossi Esteves and Heloisa Thomazi Kleina and Tiago Melo Sales and Karina Botini Liva and Juliana Balbinote and João Roberto Spotti Lopes},
url = {https://link.springer.com/article/10.1007/s42161-021-00979-y},
doi = {10.1007/S42161-021-00979-Y/METRICS},
issn = {22397264},
year = {2022},
date = {2022-01-01},
journal = {Journal of Plant Pathology},
volume = {104},
issue = {2},
pages = {487-493},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Xylella fastidiosa is a generalist phytopathogen transmitted by several polyphagous species of sharpshooters. This bacterium is responsible for plum leaf scald disease (PLS), the main phytosanitary barrier for the culture in Brazil. As an aggravating factor, weed species present in orchards can be related to the epidemiology of the disease as a source of inoculum for the pathogen. Therefore, the objective of this study was to evaluate the natural infection by X. fastidiosa in weed species present in plum orchards with a high incidence of PLS in two producing regions of Brazil, as well as to determine the colonization capacity by three sequence types (STs 26, 67 and 71) of X. fastidiosa isolated from plum trees. A total of 12 prevailing weed species were collected, nine of which (Bidens pilosa, Lepidium ruderale, Lolium multiflorum, Plantago major, Parthenium hysterosphorus, Raphanus sativus, Rumex sp., Solanum americanum and Vernonia sp.) tested positive by PCR for X. fastidiosa infection. From these, L. ruderale and L. multiflorum had the highest infection rates of 70% and 90% in root and leaf samples of L. ruderale, respectively, and 100% in leaf samples of L. multiflorum. Mechanical inoculation allowed systemic infection of the bacteria in B. pilosa, L. ruderale, R. sativus and S. americanum. Our results indicate that several weed species in plum orchards are hosts of representative STs of X. fastidiosa that infect plum, and might play a role in the spread of the pathogen to plum trees.},
keywords = {Plum leaf scald; Dissemination, Prunus salicina, Sources of inoculum},
pubstate = {published},
tppubtype = {article}
}
Xylella fastidiosa is a generalist phytopathogen transmitted by several polyphagous species of sharpshooters. This bacterium is responsible for plum leaf scald disease (PLS), the main phytosanitary barrier for the culture in Brazil. As an aggravating factor, weed species present in orchards can be related to the epidemiology of the disease as a source of inoculum for the pathogen. Therefore, the objective of this study was to evaluate the natural infection by X. fastidiosa in weed species present in plum orchards with a high incidence of PLS in two producing regions of Brazil, as well as to determine the colonization capacity by three sequence types (STs 26, 67 and 71) of X. fastidiosa isolated from plum trees. A total of 12 prevailing weed species were collected, nine of which (Bidens pilosa, Lepidium ruderale, Lolium multiflorum, Plantago major, Parthenium hysterosphorus, Raphanus sativus, Rumex sp., Solanum americanum and Vernonia sp.) tested positive by PCR for X. fastidiosa infection. From these, L. ruderale and L. multiflorum had the highest infection rates of 70% and 90% in root and leaf samples of L. ruderale, respectively, and 100% in leaf samples of L. multiflorum. Mechanical inoculation allowed systemic infection of the bacteria in B. pilosa, L. ruderale, R. sativus and S. americanum. Our results indicate that several weed species in plum orchards are hosts of representative STs of X. fastidiosa that infect plum, and might play a role in the spread of the pathogen to plum trees.
Lima, Lídia Passos; Pereira, Juliana Biar; Flores, Anthony Jhoao Fasabi; Lorenzetti, Alan Péricles Rodrigues; Boechat, Ana Laura; Pereda, Maria Claudia; Gualtieri, Sophia; Prado, Daniele Ferreira; Rocha, Diego; Ceseti, Lucas Moraes; Baldini, Regina Lúcia; Farah, Chuck S.; Koide, Tie; Benedetti, Celso Eduardo; Alvarez-Martinez, Cristina E.
An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri Journal Article
Em: Journal of Bacteriology, vol. 204, iss. 5, 2022, ISSN: 10985530.
Resumo | Links | BibTeX | Tags: phytopathogens, regulation of gene expression, sigma factors
@article{nokey,
title = {An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri},
author = {Lídia Passos Lima and Juliana Biar Pereira and Anthony Jhoao Fasabi Flores and Alan Péricles Rodrigues Lorenzetti and Ana Laura Boechat and Maria Claudia Pereda and Sophia Gualtieri and Daniele Ferreira Prado and Diego Rocha and Lucas Moraes Ceseti and Regina Lúcia Baldini and Chuck S. Farah and Tie Koide and Celso Eduardo Benedetti and Cristina E. Alvarez-Martinez},
url = {https://journals.asm.org/doi/10.1128/jb.00624-21},
doi = {10.1128/JB.00624-21/SUPPL_FILE/JB.00624-21-S0002.XLSX},
issn = {10985530},
year = {2022},
date = {2022-01-01},
journal = {Journal of Bacteriology},
volume = {204},
issue = {5},
publisher = {American Society for Microbiology},
abstract = {The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.},
keywords = {phytopathogens, regulation of gene expression, sigma factors},
pubstate = {published},
tppubtype = {article}
}
The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.
Souza-Neto, Reinaldo Rodrigues; Carvalho, Isis Gabriela Barbosa; Martins, Paula Maria Moreira; Picchi, Simone Cristina; Tomaz, Juarez Pires; Caserta, Raquel; Takita, Marco Aurélio; Souza, Alessandra Alves
MqsR toxin as a biotechnological tool for plant pathogen bacterial control Journal Article
Em: Scientific Reports 2022 12:1, vol. 12, iss. 1, pp. 1-12, 2022, ISSN: 2045-2322.
Resumo | Links | BibTeX | Tags: Antimicrobials, Molecular engineering in plants
@article{nokey,
title = {MqsR toxin as a biotechnological tool for plant pathogen bacterial control},
author = {Reinaldo Rodrigues Souza-Neto and Isis Gabriela Barbosa Carvalho and Paula Maria Moreira Martins and Simone Cristina Picchi and Juarez Pires Tomaz and Raquel Caserta and Marco Aurélio Takita and Alessandra Alves Souza},
url = {https://www.nature.com/articles/s41598-022-06690-x},
doi = {10.1038/s41598-022-06690-x},
issn = {2045-2322},
year = {2022},
date = {2022-01-01},
journal = {Scientific Reports 2022 12:1},
volume = {12},
issue = {1},
pages = {1-12},
publisher = {Nature Publishing Group},
abstract = {Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.},
keywords = {Antimicrobials, Molecular engineering in plants},
pubstate = {published},
tppubtype = {article}
}
Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.
Sousa, Andressa Rodrigues Oliveira; Ribas, Rogério Ferreira; Filho, Mauricio Antônio Coelho; Freschi, Luciano; Ferreira, Claudia Fortes; Filho, Walter Santos Soares; Pérez-Molina, Junior Pastor; Gesteira, Abelmon Silva
Drought tolerance memory transmission by citrus buds Journal Article
Em: Plant Science, vol. 320, pp. 111292, 2022, ISSN: 0168-9452.
Resumo | Links | BibTeX | Tags: Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit
@article{nokey,
title = {Drought tolerance memory transmission by citrus buds},
author = {Andressa Rodrigues Oliveira Sousa and Rogério Ferreira Ribas and Mauricio Antônio Coelho Filho and Luciano Freschi and Claudia Fortes Ferreira and Walter Santos Soares Filho and Junior Pastor Pérez-Molina and Abelmon Silva Gesteira},
doi = {10.1016/J.PLANTSCI.2022.111292},
issn = {0168-9452},
year = {2022},
date = {2022-01-01},
journal = {Plant Science},
volume = {320},
pages = {111292},
publisher = {Elsevier},
abstract = {Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.},
keywords = {Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit},
pubstate = {published},
tppubtype = {article}
}
Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.
Assis, Leticia Ribeiro; Theodoro, Reinaldo Dos Santos; Costa, Maria Beatriz Silva; Nascentes, Julyanna Andrade Silva; Rocha, Miguel Divino; Bessa, Meliza Arantes Souza; Menezes, Ralciane Paula; Dilarri, Guilherme; Hypolito, Giovane Böerner; Santos, Vanessa Rodrigues Dos; Duque, Cristiane; Ferreira, Henrique; Martins, Carlos Henrique Gomes; Regasini, Luis Octavio
Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption Journal Article
Em: Membranes, vol. 12, iss. 3, pp. 269, 2022, ISSN: 20770375.
Resumo | Links | BibTeX | Tags: Antibacterial, Biofilm, Chalcone, Membrane, Natural product
@article{nokey,
title = {Antibacterial Activity of Isobavachalcone (IBC) Is Associated with Membrane Disruption},
author = {Leticia Ribeiro Assis and Reinaldo Dos Santos Theodoro and Maria Beatriz Silva Costa and Julyanna Andrade Silva Nascentes and Miguel Divino Rocha and Meliza Arantes Souza Bessa and Ralciane Paula Menezes and Guilherme Dilarri and Giovane Böerner Hypolito and Vanessa Rodrigues Dos Santos and Cristiane Duque and Henrique Ferreira and Carlos Henrique Gomes Martins and Luis Octavio Regasini},
url = {https://www.mdpi.com/2077-0375/12/3/269/htm https://www.mdpi.com/2077-0375/12/3/269},
doi = {10.3390/MEMBRANES12030269/S1},
issn = {20770375},
year = {2022},
date = {2022-01-01},
journal = {Membranes},
volume = {12},
issue = {3},
pages = {269},
publisher = {MDPI},
abstract = {Isobavachalcone (IBC) is a natural prenylated chalcone with a broad spectrum of pharmacological properties. In this work, we newly synthesized and investigated the antibacterial activity of IBC against Gram-positive, Gram-negative and mycobacterial species. IBC was active against Grampositive bacteria, mainly against Methicillin-Susceptible Staphylococcus aureus (MSSA) and MethicillinResistant Staphylococcus aureus (MRSA), with minimum inhibitory concentration (MIC) values of 1.56 and 3.12 µg/mL, respectively. On the other hand, IBC was not able to act against Gram-negative species (MIC > 400 µg/mL). IBC displayed activity against mycobacterial species (MIC = 64 µg/mL), including Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii. IBC was able to inhibit more than 50% of MSSA and MRSA biofilm formation at 0.78 µg/mL. Its antibiofilm activity was similar to vancomycin, which was active at 0.74 µg/mL. In order to study the mechanism of the action by fluorescence microscopy, the propidium iodide (PI) and SYTO9 fluorophores indicated that IBC disrupted the membrane of Bacillus subtilis. Toxicity assays using human keratinocytes (HaCaT cell line) showed that IBC did not have the capacity to reduce the cell viability. These results suggested that IBC is a promising antibacterial agent with an elucidated mode of action and potential applications as an antibacterial drug and a medical device coating.},
keywords = {Antibacterial, Biofilm, Chalcone, Membrane, Natural product},
pubstate = {published},
tppubtype = {article}
}
Isobavachalcone (IBC) is a natural prenylated chalcone with a broad spectrum of pharmacological properties. In this work, we newly synthesized and investigated the antibacterial activity of IBC against Gram-positive, Gram-negative and mycobacterial species. IBC was active against Grampositive bacteria, mainly against Methicillin-Susceptible Staphylococcus aureus (MSSA) and MethicillinResistant Staphylococcus aureus (MRSA), with minimum inhibitory concentration (MIC) values of 1.56 and 3.12 µg/mL, respectively. On the other hand, IBC was not able to act against Gram-negative species (MIC > 400 µg/mL). IBC displayed activity against mycobacterial species (MIC = 64 µg/mL), including Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii. IBC was able to inhibit more than 50% of MSSA and MRSA biofilm formation at 0.78 µg/mL. Its antibiofilm activity was similar to vancomycin, which was active at 0.74 µg/mL. In order to study the mechanism of the action by fluorescence microscopy, the propidium iodide (PI) and SYTO9 fluorophores indicated that IBC disrupted the membrane of Bacillus subtilis. Toxicity assays using human keratinocytes (HaCaT cell line) showed that IBC did not have the capacity to reduce the cell viability. These results suggested that IBC is a promising antibacterial agent with an elucidated mode of action and potential applications as an antibacterial drug and a medical device coating.
Bettini-Tambur, Bruna Aparecida; Azevedo, Fernando Alves; Cristofani-Yaly, Mariângela; Soares, Maria Beatriz Bernardes; Martins, Mônica Helena; Schinor, Evandro Henrique
Degree days, phenology and fruit quality of ‘Tahiti IAC-5’ acid lime grafted onto different rootstocks in Pindorama - SP Journal Article
Em: Revista Brasileira de Fruticultura, vol. 44, iss. 1, 2022, ISSN: 0100-2945.
Resumo | Links | BibTeX | Tags: ‘Rangpur’ lime, citrandarin, Citrus × latifolia, Poncirus trifoliata ‘Flying Dragon
@article{nokey,
title = {Degree days, phenology and fruit quality of ‘Tahiti IAC-5’ acid lime grafted onto different rootstocks in Pindorama - SP},
author = {Bruna Aparecida Bettini-Tambur and Fernando Alves Azevedo and Mariângela Cristofani-Yaly and Maria Beatriz Bernardes Soares and Mônica Helena Martins and Evandro Henrique Schinor},
url = {http://www.scielo.br/j/rbf/a/p4vtqH6vMH78hHQzFfKpZtN/},
doi = {10.1590/0100-29452022064},
issn = {0100-2945},
year = {2022},
date = {2022-01-01},
journal = {Revista Brasileira de Fruticultura},
volume = {44},
issue = {1},
publisher = {Sociedade Brasileira de Fruticultura},
abstract = {Abstract Few rootstock varieties are used for ‘Tahiti’ acid lime; therefore, diversification is necessary and studying the phenological phases of new scion/rootstock combinations is important for orchard management strategies. The aim of this study was to evaluate the influence of different rootstocks on the sum of degree days (DD) and on the phenology of plants and also on the quality of ‘Tahiti’ acid lime fruits, in Pindorama, SP. Six branches were marked on plants grafted onto 13 citrandarins (H), ‘Rangpur’ lime (RL), ‘Flying Dragon’ trifoliate orange (FD) and ‘Sunki’ mandarin (SM). Phenological characterization (anthesis to harvest) was performed using a grading scale. DD accumulation (anthesis to harvest) was determined considering the base temperature of 13 °C. Physical (weight, height and diameter, juice yield) and chemical (acidity, total soluble solids and ratio) characteristics of fruits were also evaluated. The experimental design was in randomized blocks with three replicates and one plant per plot. The rootstocks under study influenced DD accumulation and phenology, with early (H5, H73, H121, H135, H145, H152, FD - 1,558 at 1,676 DD and 135 to 146 days) and later combinations (H10, H14, H42, H47, H70, H150, H173, RL and SM - 1,694 to 1,829 DD and 148 to 157 days) regarding fruit maturation. The harvest point of ‘Tahiti’ acid lime fruits grafted onto H145 citrandarin and FD was advanced about 20 days in relation to the standard rootstock, RL. Total soluble solids content and fruit size were influenced by rootstocks; however, all scion/rootstock combinations presented fruits with quality characteristics within commercial standards.},
keywords = {‘Rangpur’ lime, citrandarin, Citrus × latifolia, Poncirus trifoliata ‘Flying Dragon},
pubstate = {published},
tppubtype = {article}
}
Abstract Few rootstock varieties are used for ‘Tahiti’ acid lime; therefore, diversification is necessary and studying the phenological phases of new scion/rootstock combinations is important for orchard management strategies. The aim of this study was to evaluate the influence of different rootstocks on the sum of degree days (DD) and on the phenology of plants and also on the quality of ‘Tahiti’ acid lime fruits, in Pindorama, SP. Six branches were marked on plants grafted onto 13 citrandarins (H), ‘Rangpur’ lime (RL), ‘Flying Dragon’ trifoliate orange (FD) and ‘Sunki’ mandarin (SM). Phenological characterization (anthesis to harvest) was performed using a grading scale. DD accumulation (anthesis to harvest) was determined considering the base temperature of 13 °C. Physical (weight, height and diameter, juice yield) and chemical (acidity, total soluble solids and ratio) characteristics of fruits were also evaluated. The experimental design was in randomized blocks with three replicates and one plant per plot. The rootstocks under study influenced DD accumulation and phenology, with early (H5, H73, H121, H135, H145, H152, FD - 1,558 at 1,676 DD and 135 to 146 days) and later combinations (H10, H14, H42, H47, H70, H150, H173, RL and SM - 1,694 to 1,829 DD and 148 to 157 days) regarding fruit maturation. The harvest point of ‘Tahiti’ acid lime fruits grafted onto H145 citrandarin and FD was advanced about 20 days in relation to the standard rootstock, RL. Total soluble solids content and fruit size were influenced by rootstocks; however, all scion/rootstock combinations presented fruits with quality characteristics within commercial standards.
Vieira, Gabrielle; Khalil, Zeinab G.; Capon, Robert J.; Sette, Lara D.; Ferreira, Henrique; Sass, Daiane C.
Isolation and agricultural potential of penicillic acid against citrus canker Journal Article
Em: Journal of Applied Microbiology, vol. 132, iss. 4, pp. 3081-3088, 2022, ISSN: 1365-2672.
Resumo | Links | BibTeX | Tags: agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases
@article{Vieira2022,
title = {Isolation and agricultural potential of penicillic acid against citrus canker},
author = {Gabrielle Vieira and Zeinab G. Khalil and Robert J. Capon and Lara D. Sette and Henrique Ferreira and Daiane C. Sass},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15413 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15413 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15413},
doi = {10.1111/JAM.15413},
issn = {1365-2672},
year = {2022},
date = {2022-01-01},
journal = {Journal of Applied Microbiology},
volume = {132},
issue = {4},
pages = {3081-3088},
publisher = {John Wiley & Sons, Ltd},
abstract = {Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.},
keywords = {agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases},
pubstate = {published},
tppubtype = {article}
}
Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.
Varano, A.; Shirahigue, L. D.; Azevedo, F. A.; Silva, M. Altenhofen; Ceccato-Antonini, S. R.
Mandarin essential oil as an antimicrobial in ethanolic fermentation: Effects on Limosilactobacillus fermentum and Saccharomyces cerevisiae Journal Article
Em: Letters in Applied Microbiology, vol. 74, iss. 6, pp. 981-991, 2022, ISSN: 1472-765X.
Resumo | Links | BibTeX | Tags: bacterial contamination, bioethanol, citrus essential oil, microbiocide, yeast inhibition
@article{Varano2022,
title = {Mandarin essential oil as an antimicrobial in ethanolic fermentation: Effects on Limosilactobacillus fermentum and Saccharomyces cerevisiae},
author = {A. Varano and L. D. Shirahigue and F. A. Azevedo and M. Altenhofen Silva and S. R. Ceccato-Antonini},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/lam.13690 https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.13690 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/lam.13690},
doi = {10.1111/LAM.13690},
issn = {1472-765X},
year = {2022},
date = {2022-01-01},
journal = {Letters in Applied Microbiology},
volume = {74},
issue = {6},
pages = {981-991},
publisher = {John Wiley & Sons, Ltd},
abstract = {The antibacterial activity of citrus essential oils (EOs) in the context of combating Limosilactobacillus fermentum, one of the most important bacterial contaminants in the bioethanol production industry, has never been explored previously. Industrial processes usually utilize sulfuric acid for cell treatment to decrease bacterial contamination. However, due to the hazardous nature of sulfuric acid, an alternative to it is highly desirable. Therefore, in the present study, the efficacy of Fremont IAC 543 mandarin EO against a strain of L. fermentum (ATCC® 9338™) was evaluated under proliferative/nonproliferative conditions, in both pure culture and co-culture with an industrial strain of Saccharomyces cerevisiae. The mandarin EO exhibited higher effectiveness against L. fermentum compared to that against S. cerevisiae under nonproliferative conditions (added to water rather than to culture medium). At the concentration of 0·05%, the EO was as effective as the acid solution with pH 2·0 in reducing the count of L. fermentum almost 5 log CFU ml–1 cycles, while the concentration of 0·1% led to the complete loss of bacterial culturability. When L. fermentum was co-cultured with S. cerevisiae, the efficacy of the EO against the bacterial strain was reduced. However, despite this reduced efficacy in co-culture, mandarin EO may be considered effective in combating L. fermentum and could be applied in processes where this bacterium proves to be unfavourable and does not interact with S. cerevisiae.},
keywords = {bacterial contamination, bioethanol, citrus essential oil, microbiocide, yeast inhibition},
pubstate = {published},
tppubtype = {article}
}
The antibacterial activity of citrus essential oils (EOs) in the context of combating Limosilactobacillus fermentum, one of the most important bacterial contaminants in the bioethanol production industry, has never been explored previously. Industrial processes usually utilize sulfuric acid for cell treatment to decrease bacterial contamination. However, due to the hazardous nature of sulfuric acid, an alternative to it is highly desirable. Therefore, in the present study, the efficacy of Fremont IAC 543 mandarin EO against a strain of L. fermentum (ATCC® 9338™) was evaluated under proliferative/nonproliferative conditions, in both pure culture and co-culture with an industrial strain of Saccharomyces cerevisiae. The mandarin EO exhibited higher effectiveness against L. fermentum compared to that against S. cerevisiae under nonproliferative conditions (added to water rather than to culture medium). At the concentration of 0·05%, the EO was as effective as the acid solution with pH 2·0 in reducing the count of L. fermentum almost 5 log CFU ml–1 cycles, while the concentration of 0·1% led to the complete loss of bacterial culturability. When L. fermentum was co-cultured with S. cerevisiae, the efficacy of the EO against the bacterial strain was reduced. However, despite this reduced efficacy in co-culture, mandarin EO may be considered effective in combating L. fermentum and could be applied in processes where this bacterium proves to be unfavourable and does not interact with S. cerevisiae.
Sousa, Andressa Rodrigues Oliveira; Silva, Edson Mario Andrade; Filho, Maurício Antônio Coelho; Costa, Marcio Gilberto Cardoso; Filho, Walter Santos Soares; Micheli, Fabienne; Maserti, Biancaelena; Gesteira, Abelmon Silva
Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations Journal Article
Em: Scientia Horticulturae, vol. 292, pp. 110490, 2022, ISSN: 0304-4238.
Resumo | Links | BibTeX | Tags: Amino acids, Carbohydrates, Citrus, drought, Metabolic profile, rootstock
@article{Sousa2022,
title = {Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations},
author = {Andressa Rodrigues Oliveira Sousa and Edson Mario Andrade Silva and Maurício Antônio Coelho Filho and Marcio Gilberto Cardoso Costa and Walter Santos Soares Filho and Fabienne Micheli and Biancaelena Maserti and Abelmon Silva Gesteira},
doi = {10.1016/J.SCIENTA.2021.110490},
issn = {0304-4238},
year = {2022},
date = {2022-01-01},
journal = {Scientia Horticulturae},
volume = {292},
pages = {110490},
publisher = {Elsevier},
abstract = {Drought is one of the most destructive environmental challenges for agriculture, and citrus trees are cultivated in semiarid areas. The current study used physiological and untarget mass spectrometry analysis to assess the differential response to drought and rehydration in leaves and roots of three scion/rootstock combinations in which the same scion Valencia Orange was grafted onto three different rootstocks, namely, ‘Rangpur Lime’, ‘Sunki Maravilha’ and ‘Sunki Tropical’, known to have a different response to drought. The main question to be answer was whether the soil disease tolerant ‘Sunki Tropical’ could be used as alternative to the drought tolerant/disease susceptible rootstock ‘Rangpur lime’ to cultivate citrus in semiarid areas. Thirty-seven metabolites in leaves and forty-six in roots, mainly amino acids, as well as a few carbohydrates and organic acids were found differentially expressed after drought and/or rehydration in the three combinations. Overall, the results demonstrated that the three scion/rootstock combinations responded differently at metabolite levels to water changes. The few leaf metabolites found in the scion grafted on ‘Rangpur Lime’ and ‘Sunki Maravilha’ support the respective drought avoidance and tolerance strategy, already observed in those rootstocks.‘Rangur Lime’ and ‘Sunki Tropical’ shared at roots levels several common drought response mechanisms. Moreover, the high number of differential metabolites triggered in the scion by 'Sunki Tropical' seems to be useful for overcoming the drought effects without depleting the fruit quality. From our data, we argue that ‘Sunki Tropical’ is a drought tolerant genotypes that could be used as alternative to ‘Ranpur Lime’ to sustain citrus production in semiarid areas.},
keywords = {Amino acids, Carbohydrates, Citrus, drought, Metabolic profile, rootstock},
pubstate = {published},
tppubtype = {article}
}
Drought is one of the most destructive environmental challenges for agriculture, and citrus trees are cultivated in semiarid areas. The current study used physiological and untarget mass spectrometry analysis to assess the differential response to drought and rehydration in leaves and roots of three scion/rootstock combinations in which the same scion Valencia Orange was grafted onto three different rootstocks, namely, ‘Rangpur Lime’, ‘Sunki Maravilha’ and ‘Sunki Tropical’, known to have a different response to drought. The main question to be answer was whether the soil disease tolerant ‘Sunki Tropical’ could be used as alternative to the drought tolerant/disease susceptible rootstock ‘Rangpur lime’ to cultivate citrus in semiarid areas. Thirty-seven metabolites in leaves and forty-six in roots, mainly amino acids, as well as a few carbohydrates and organic acids were found differentially expressed after drought and/or rehydration in the three combinations. Overall, the results demonstrated that the three scion/rootstock combinations responded differently at metabolite levels to water changes. The few leaf metabolites found in the scion grafted on ‘Rangpur Lime’ and ‘Sunki Maravilha’ support the respective drought avoidance and tolerance strategy, already observed in those rootstocks.‘Rangur Lime’ and ‘Sunki Tropical’ shared at roots levels several common drought response mechanisms. Moreover, the high number of differential metabolites triggered in the scion by 'Sunki Tropical' seems to be useful for overcoming the drought effects without depleting the fruit quality. From our data, we argue that ‘Sunki Tropical’ is a drought tolerant genotypes that could be used as alternative to ‘Ranpur Lime’ to sustain citrus production in semiarid areas.
Sinico, Thaís Elise; Nunes, Maria Andréia; Kitajima, Elliot Watanabe; Cunha, Bruna Aparecida; Novelli, Valdenice Moreira
Notes on the embryological development of the Brevipalpus yothersi (Acari: Tenuipalpidae) Journal Article
Em: Acarologia, vol. 62, iss. 1, pp. 113-119, 2022, ISSN: 0044-586X.
Resumo | Links | BibTeX | Tags: Acari, Acariformes, Animalia, Arachnida, Arthropoda, citrus leprosis disease, embryology, mite, phytophagous mite, vector
@article{Sinico2022,
title = {Notes on the embryological development of the Brevipalpus yothersi (Acari: Tenuipalpidae)},
author = {Thaís Elise Sinico and Maria Andréia Nunes and Elliot Watanabe Kitajima and Bruna Aparecida Cunha and Valdenice Moreira Novelli},
url = {https://www1.montpellier.inra.fr/CBGP/acarologia/article.php?id=4496},
doi = {10.24349/0GR3-3A6B},
issn = {0044-586X},
year = {2022},
date = {2022-01-01},
journal = {Acarologia},
volume = {62},
issue = {1},
pages = {113-119},
publisher = {Les Amis d'Acarologia},
abstract = {Mites of the Brevipalpus genus are economically important due to their role as vectors of plant viruses. Among them, B. yothersi is well studied because it is the recognized main vector of the citrus leprosis virus C (CiLV-C), the causal agent of citrus leprosis, which is one of the most damaging viral diseases in the citrus crops. However, studies of several aspects of the biology of B. yothersi are still scarce, including its embryology. We performed a descriptive embryonic development study of B. yothersi, utilizing light and scanning electron microscope to obtain biological information of this particular phytovirus vector. Distinct events were recorded, such as centering of the nucleus, mitotic divisions for primitive body formation and appendages, as well as the presence of channels inside the egg and their possible association with the respiratory tract. Here we provide the first notes about embryonic development of this important mite vector in the Americas.},
keywords = {Acari, Acariformes, Animalia, Arachnida, Arthropoda, citrus leprosis disease, embryology, mite, phytophagous mite, vector},
pubstate = {published},
tppubtype = {article}
}
Mites of the Brevipalpus genus are economically important due to their role as vectors of plant viruses. Among them, B. yothersi is well studied because it is the recognized main vector of the citrus leprosis virus C (CiLV-C), the causal agent of citrus leprosis, which is one of the most damaging viral diseases in the citrus crops. However, studies of several aspects of the biology of B. yothersi are still scarce, including its embryology. We performed a descriptive embryonic development study of B. yothersi, utilizing light and scanning electron microscope to obtain biological information of this particular phytovirus vector. Distinct events were recorded, such as centering of the nucleus, mitotic divisions for primitive body formation and appendages, as well as the presence of channels inside the egg and their possible association with the respiratory tract. Here we provide the first notes about embryonic development of this important mite vector in the Americas.
Shimizu, Gabriel Danilo; Paula, Jean Carlo Baudraz; Silva, Adriana Pereira; Pacheco, Camilla Andrade; Azevedo, Fernando Alves; Gonçalves, Leandro Simões Azeredo; Neves, Carmen Silvia Vieira Janeiro
Chemical and microbiological changes in the soil mediated by different vegetative coverings in a Natal orange orchard Journal Article
Em: Semina: Ciências Agrárias, vol. 43, iss. 1, pp. 331-350, 2022, ISSN: 1679-0359.
Resumo | Links | BibTeX | Tags: Citrus sinensis, conservation agriculture, Glyphosate, Urochloa.
@article{Shimizu2022,
title = {Chemical and microbiological changes in the soil mediated by different vegetative coverings in a Natal orange orchard},
author = {Gabriel Danilo Shimizu and Jean Carlo Baudraz Paula and Adriana Pereira Silva and Camilla Andrade Pacheco and Fernando Alves Azevedo and Leandro Simões Azeredo Gonçalves and Carmen Silvia Vieira Janeiro Neves},
url = {https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44132},
doi = {10.5433/1679-0359.2022V43N1P331},
issn = {1679-0359},
year = {2022},
date = {2022-01-01},
journal = {Semina: Ciências Agrárias},
volume = {43},
issue = {1},
pages = {331-350},
publisher = {Universidade Estadual de Londrina},
abstract = {Proper soil cover management for citrus cultivation can contribute to increased productivity and improved soil quality. This study examined five different vegetative coverings [Urochloa brizantha; U. decumbens, U. ruziziensis, spontaneous vegetation, and herbicide application (glyphosate) in the total area] in the inter rows of a Natal orange orchard [Citrus sinensis (L.) Osbeck] grafted on the Swingle citrumelo (C. paradisi × Poncirus trifoliata). Their effects on the microbiological and chemical attributes of the soil and the vegetative development in the orchard were examined. Chemical (Ca2+, Mg2+, K+, P, pH, H+Al, CECpH7, base saturation, and OM) and microbiological (carbon and nitrogen of microbial biomass, basal respiration, and metabolic quotient) soil attributes in the rows and inter-rows were evaluated for the orchard in 2018 and 2019. There was a significant difference for most variables in the 2 years studied, emphasizing 2019 for microbiological parameters and OM, with the latter being 14.8% lower in the treatment with glyphosate in the total area compared to the treatment with spontaneous vegetation. The results showed the benefits of vegetation cover with brachiaria in inter-rows of the Natal sweet orange orchard in the chemical and microbiological attributes of the soil, especially in carbon and nitrogen of the microbial biomass.},
keywords = {Citrus sinensis, conservation agriculture, Glyphosate, Urochloa.},
pubstate = {published},
tppubtype = {article}
}
Proper soil cover management for citrus cultivation can contribute to increased productivity and improved soil quality. This study examined five different vegetative coverings [Urochloa brizantha; U. decumbens, U. ruziziensis, spontaneous vegetation, and herbicide application (glyphosate) in the total area] in the inter rows of a Natal orange orchard [Citrus sinensis (L.) Osbeck] grafted on the Swingle citrumelo (C. paradisi × Poncirus trifoliata). Their effects on the microbiological and chemical attributes of the soil and the vegetative development in the orchard were examined. Chemical (Ca2+, Mg2+, K+, P, pH, H+Al, CECpH7, base saturation, and OM) and microbiological (carbon and nitrogen of microbial biomass, basal respiration, and metabolic quotient) soil attributes in the rows and inter-rows were evaluated for the orchard in 2018 and 2019. There was a significant difference for most variables in the 2 years studied, emphasizing 2019 for microbiological parameters and OM, with the latter being 14.8% lower in the treatment with glyphosate in the total area compared to the treatment with spontaneous vegetation. The results showed the benefits of vegetation cover with brachiaria in inter-rows of the Natal sweet orange orchard in the chemical and microbiological attributes of the soil, especially in carbon and nitrogen of the microbial biomass.
Nunes, Maria Andréia; Ferreira, Leticia Maria; Rogerio, Luana Aparecida; Kitajima, Elliot Watanabe; Novelli, Valdenice Moreira
Infection by coffee ringspot virus is identified for the first time in succulent plants Journal Article
Em: Journal of Plant Pathology, vol. 104, iss. 2, pp. 795-799, 2022, ISSN: 22397264.
Resumo | Links | BibTeX | Tags: Brevipalpus, Coffee pathogens, Emergent plant virus, Virus transmitted by mites
@article{Nunes2022,
title = {Infection by coffee ringspot virus is identified for the first time in succulent plants},
author = {Maria Andréia Nunes and Leticia Maria Ferreira and Luana Aparecida Rogerio and Elliot Watanabe Kitajima and Valdenice Moreira Novelli},
url = {https://link.springer.com/article/10.1007/s42161-022-01100-7},
doi = {10.1007/S42161-022-01100-7/METRICS},
issn = {22397264},
year = {2022},
date = {2022-01-01},
journal = {Journal of Plant Pathology},
volume = {104},
issue = {2},
pages = {795-799},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Coffee ringspot virus (CoRSV) belongs to the Dichorhavirus genus and has been considered the causal agent of the emergent disease in commercial coffee orchards. The virus is commonly transmitted by Brevipalpus papayensis Baker (Acari: Tenuipalpidae) in a persistent propagative mode, however B. yothersi is another mite vector. Here, we report for the first time that the common succulent panda plant (Kalanchoe tomentosa Baker) and bear’s paw (Cotyledon tomentosa Harv.) can be infected with CoRSV transmitted by viruliferous B. papayensis females. Leaves showed typical symptoms of chlorotic and necrotic spots seven days after inoculation and RT-PCR confirmed the presence of CoRSV in symptomatic tissues. Amplicon sequences showed > 97% identity with a known CoRSV sequence (Genbank accession number GQ979998). In addition, transmission electron microscopy revealed the presence of typical dichorhavirus particles in the nucleus of symptomatic tissues of panda plants. Thus, considering the relevance of succulents in the ornamental market and the emergent CoRSV, it is important to provide information about the effects of this virus in different hosts.},
keywords = {Brevipalpus, Coffee pathogens, Emergent plant virus, Virus transmitted by mites},
pubstate = {published},
tppubtype = {article}
}
Coffee ringspot virus (CoRSV) belongs to the Dichorhavirus genus and has been considered the causal agent of the emergent disease in commercial coffee orchards. The virus is commonly transmitted by Brevipalpus papayensis Baker (Acari: Tenuipalpidae) in a persistent propagative mode, however B. yothersi is another mite vector. Here, we report for the first time that the common succulent panda plant (Kalanchoe tomentosa Baker) and bear’s paw (Cotyledon tomentosa Harv.) can be infected with CoRSV transmitted by viruliferous B. papayensis females. Leaves showed typical symptoms of chlorotic and necrotic spots seven days after inoculation and RT-PCR confirmed the presence of CoRSV in symptomatic tissues. Amplicon sequences showed > 97% identity with a known CoRSV sequence (Genbank accession number GQ979998). In addition, transmission electron microscopy revealed the presence of typical dichorhavirus particles in the nucleus of symptomatic tissues of panda plants. Thus, considering the relevance of succulents in the ornamental market and the emergent CoRSV, it is important to provide information about the effects of this virus in different hosts.
Nascimento, Cesar Augusto; Teixeira-Silva, Natalia Sousa; Caserta, Raquel; Marques, Marcia Ortiz Mayo; Takita, Marco Aurelio; Souza, Alessandra A.
Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri Journal Article
Em: Frontiers in Plant Science, vol. 13, pp. 820, 2022, ISSN: 1664462X.
Resumo | Links | BibTeX | Tags: Citrus canker, MeSA, Methyl salicylate, Salicylic acid, systemic-acquired resistance
@article{Nascimento2022,
title = {Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri},
author = {Cesar Augusto Nascimento and Natalia Sousa Teixeira-Silva and Raquel Caserta and Marcia Ortiz Mayo Marques and Marco Aurelio Takita and Alessandra A. Souza},
doi = {10.3389/FPLS.2022.836582/BIBTEX},
issn = {1664462X},
year = {2022},
date = {2022-01-01},
journal = {Frontiers in Plant Science},
volume = {13},
pages = {820},
publisher = {Frontiers Media S.A.},
abstract = {Citrus canker is a destructive disease caused by Xanthomonas citri subsp. citri, which affects all commercial sweet orange (Citrus sinensis [L.] Osbeck) cultivars. Salicylic acid (SA) and systemic-acquired resistance (SAR) have been demonstrated to have a crucial role in mediating plant defense responses against this phytopathogen. To induce SAR, SA is converted to methyl salicylate (MeSA) by an SA-dependent methyltransferase (SAMT) and translocated systemically to prime noninfected distal tissues. Here, we generated sweet orange transgenic plants (based on cvs. Hamlin and Valencia) overexpressing the SAMT gene from Citrus (CsSAMT) and evaluated their resistance to citrus canker. We obtained four independent transgenic lines and confirmed their significantly higher MeSA volatilization compared to wild-type controls. Plants overexpressing CsSAMT showed reduced symptoms of citrus canker and bacterial populations in all transgenic lines without compromising plant development. One representative transgenic line (V44SAMT) was used to evaluate resistance response in primary and secondary sites. Without inoculation, V44SAMT modulated CsSAMT, CsNPR1, CsNPR3, and CsWRKY22 expression, indicating that this plant is in a primed defense status. The results demonstrate that MeSA signaling prompts the plant to respond more efficiently to pathogen attacks and induces immune responses in transgenic plants at both primary and secondary infection sites.},
keywords = {Citrus canker, MeSA, Methyl salicylate, Salicylic acid, systemic-acquired resistance},
pubstate = {published},
tppubtype = {article}
}
Citrus canker is a destructive disease caused by Xanthomonas citri subsp. citri, which affects all commercial sweet orange (Citrus sinensis [L.] Osbeck) cultivars. Salicylic acid (SA) and systemic-acquired resistance (SAR) have been demonstrated to have a crucial role in mediating plant defense responses against this phytopathogen. To induce SAR, SA is converted to methyl salicylate (MeSA) by an SA-dependent methyltransferase (SAMT) and translocated systemically to prime noninfected distal tissues. Here, we generated sweet orange transgenic plants (based on cvs. Hamlin and Valencia) overexpressing the SAMT gene from Citrus (CsSAMT) and evaluated their resistance to citrus canker. We obtained four independent transgenic lines and confirmed their significantly higher MeSA volatilization compared to wild-type controls. Plants overexpressing CsSAMT showed reduced symptoms of citrus canker and bacterial populations in all transgenic lines without compromising plant development. One representative transgenic line (V44SAMT) was used to evaluate resistance response in primary and secondary sites. Without inoculation, V44SAMT modulated CsSAMT, CsNPR1, CsNPR3, and CsWRKY22 expression, indicating that this plant is in a primed defense status. The results demonstrate that MeSA signaling prompts the plant to respond more efficiently to pathogen attacks and induces immune responses in transgenic plants at both primary and secondary infection sites.
Müller, Cristiane; Esteves, Mariana Bossi; Kleina, Heloisa Thomazi; Sales, Tiago Melo; Liva, Karina Botini; Balbinote, Juliana; Lopes, João Roberto Spotti
Weeds as alternative hosts of Xylella fastidiosa in Brazilian plum orchards Journal Article
Em: Journal of Plant Pathology, vol. 104, iss. 2, pp. 487-493, 2022, ISSN: 22397264.
Resumo | Links | BibTeX | Tags: Plum leaf scald; Dissemination, Prunus salicina, Sources of inoculum
@article{nokey,
title = {Weeds as alternative hosts of Xylella fastidiosa in Brazilian plum orchards},
author = {Cristiane Müller and Mariana Bossi Esteves and Heloisa Thomazi Kleina and Tiago Melo Sales and Karina Botini Liva and Juliana Balbinote and João Roberto Spotti Lopes},
url = {https://link.springer.com/article/10.1007/s42161-021-00979-y},
doi = {10.1007/S42161-021-00979-Y/METRICS},
issn = {22397264},
year = {2022},
date = {2022-01-01},
journal = {Journal of Plant Pathology},
volume = {104},
issue = {2},
pages = {487-493},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Xylella fastidiosa is a generalist phytopathogen transmitted by several polyphagous species of sharpshooters. This bacterium is responsible for plum leaf scald disease (PLS), the main phytosanitary barrier for the culture in Brazil. As an aggravating factor, weed species present in orchards can be related to the epidemiology of the disease as a source of inoculum for the pathogen. Therefore, the objective of this study was to evaluate the natural infection by X. fastidiosa in weed species present in plum orchards with a high incidence of PLS in two producing regions of Brazil, as well as to determine the colonization capacity by three sequence types (STs 26, 67 and 71) of X. fastidiosa isolated from plum trees. A total of 12 prevailing weed species were collected, nine of which (Bidens pilosa, Lepidium ruderale, Lolium multiflorum, Plantago major, Parthenium hysterosphorus, Raphanus sativus, Rumex sp., Solanum americanum and Vernonia sp.) tested positive by PCR for X. fastidiosa infection. From these, L. ruderale and L. multiflorum had the highest infection rates of 70% and 90% in root and leaf samples of L. ruderale, respectively, and 100% in leaf samples of L. multiflorum. Mechanical inoculation allowed systemic infection of the bacteria in B. pilosa, L. ruderale, R. sativus and S. americanum. Our results indicate that several weed species in plum orchards are hosts of representative STs of X. fastidiosa that infect plum, and might play a role in the spread of the pathogen to plum trees.},
keywords = {Plum leaf scald; Dissemination, Prunus salicina, Sources of inoculum},
pubstate = {published},
tppubtype = {article}
}
Xylella fastidiosa is a generalist phytopathogen transmitted by several polyphagous species of sharpshooters. This bacterium is responsible for plum leaf scald disease (PLS), the main phytosanitary barrier for the culture in Brazil. As an aggravating factor, weed species present in orchards can be related to the epidemiology of the disease as a source of inoculum for the pathogen. Therefore, the objective of this study was to evaluate the natural infection by X. fastidiosa in weed species present in plum orchards with a high incidence of PLS in two producing regions of Brazil, as well as to determine the colonization capacity by three sequence types (STs 26, 67 and 71) of X. fastidiosa isolated from plum trees. A total of 12 prevailing weed species were collected, nine of which (Bidens pilosa, Lepidium ruderale, Lolium multiflorum, Plantago major, Parthenium hysterosphorus, Raphanus sativus, Rumex sp., Solanum americanum and Vernonia sp.) tested positive by PCR for X. fastidiosa infection. From these, L. ruderale and L. multiflorum had the highest infection rates of 70% and 90% in root and leaf samples of L. ruderale, respectively, and 100% in leaf samples of L. multiflorum. Mechanical inoculation allowed systemic infection of the bacteria in B. pilosa, L. ruderale, R. sativus and S. americanum. Our results indicate that several weed species in plum orchards are hosts of representative STs of X. fastidiosa that infect plum, and might play a role in the spread of the pathogen to plum trees.
Miranda, Marcela T.; Espinoza-Núñez, Erick; Silva, Simone F.; Boscariol-Camargo, Raquel L.; Machado, Eduardo C.; Ribeiro, Rafael V.
Leaf PIP2.1 and PIP2.5 are down-regulated in ‘Mandarin’ lime under drought Journal Article
Em: Theoretical and Experimental Plant Physiology, vol. 34, iss. 1, pp. 63-69, 2022, ISSN: 21970025.
Resumo | Links | BibTeX | Tags: ‘Swingle’ citrumelo, ‘Valencia’ sweet orange, Aquaporins, Leaf gas exchange, Rootstocks, Stomatal conductance
@article{Miranda2022,
title = {Leaf PIP2.1 and PIP2.5 are down-regulated in ‘Mandarin’ lime under drought},
author = {Marcela T. Miranda and Erick Espinoza-Núñez and Simone F. Silva and Raquel L. Boscariol-Camargo and Eduardo C. Machado and Rafael V. Ribeiro},
url = {https://link.springer.com/article/10.1007/s40626-021-00229-7},
doi = {10.1007/S40626-021-00229-7/METRICS},
issn = {21970025},
year = {2022},
date = {2022-01-01},
journal = {Theoretical and Experimental Plant Physiology},
volume = {34},
issue = {1},
pages = {63-69},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Aquaporins are water channel proteins that facilitate water transport through the transcellular pathway, a route with high resistance due to lipid bilayer membranes. Aquaporins occur as multiple isoforms and plasma membrane aquaporins (PIPs) are known to modulate plant responses to abiotic stress such as drought. Previous studies have reported changes in PIPs expression in roots of Citrus plants under abiotic stresses; however, such changes are limited to grafted citrus trees with plant responses to environmental stresses being driven by two species. Therefore, the ability of a given Citrus species—i.e., non-grafted Citrus saplings—in modulating aquaporin expression under water deficit remains unknown. This study aimed to evaluate how water deficit changes aquaporin expression (PIP1; PIP2.1; and PIP2.5) and leaf gas exchange of saplings of three Citrus species: ‘Mandarin’ lime, ‘Swingle’ citrumelo and ‘Valencia’ sweet orange. Root aquaporin expression did not vary under water deficit or among species, but the predawn leaf water potential of ‘Valencia’ sweet orange was reduced due to water deficit. Decreases in stomatal conductance were found in all species under water deficit, which were associated with reduced PIP2.1 expression in leaves. However, ‘Mandarin’ lime exhibited the largest reduction in stomatal conductance and significant gene repression of not only PIP2.1 but also PIP2.5 in leaves under water deficit. ‘Mandarin’ lime is known as a drought tolerant rootstock widely used in citriculture and our data suggest that this rootstock presents a fast-response mechanism against drought based on stomatal closure associated with downregulation of PIP2.1 and PIP2.5 in leaves.},
keywords = {‘Swingle’ citrumelo, ‘Valencia’ sweet orange, Aquaporins, Leaf gas exchange, Rootstocks, Stomatal conductance},
pubstate = {published},
tppubtype = {article}
}
Aquaporins are water channel proteins that facilitate water transport through the transcellular pathway, a route with high resistance due to lipid bilayer membranes. Aquaporins occur as multiple isoforms and plasma membrane aquaporins (PIPs) are known to modulate plant responses to abiotic stress such as drought. Previous studies have reported changes in PIPs expression in roots of Citrus plants under abiotic stresses; however, such changes are limited to grafted citrus trees with plant responses to environmental stresses being driven by two species. Therefore, the ability of a given Citrus species—i.e., non-grafted Citrus saplings—in modulating aquaporin expression under water deficit remains unknown. This study aimed to evaluate how water deficit changes aquaporin expression (PIP1; PIP2.1; and PIP2.5) and leaf gas exchange of saplings of three Citrus species: ‘Mandarin’ lime, ‘Swingle’ citrumelo and ‘Valencia’ sweet orange. Root aquaporin expression did not vary under water deficit or among species, but the predawn leaf water potential of ‘Valencia’ sweet orange was reduced due to water deficit. Decreases in stomatal conductance were found in all species under water deficit, which were associated with reduced PIP2.1 expression in leaves. However, ‘Mandarin’ lime exhibited the largest reduction in stomatal conductance and significant gene repression of not only PIP2.1 but also PIP2.5 in leaves under water deficit. ‘Mandarin’ lime is known as a drought tolerant rootstock widely used in citriculture and our data suggest that this rootstock presents a fast-response mechanism against drought based on stomatal closure associated with downregulation of PIP2.1 and PIP2.5 in leaves.