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2022
Souza-Neto, Reinaldo Rodrigues; Carvalho, Isis Gabriela Barbosa; Martins, Paula Maria Moreira; Picchi, Simone Cristina; Tomaz, Juarez Pires; Caserta, Raquel; Takita, Marco Aurélio; Souza, Alessandra Alves
MqsR toxin as a biotechnological tool for plant pathogen bacterial control Journal Article
Em: Scientific Reports 2022 12:1, vol. 12, iss. 1, pp. 1-12, 2022, ISSN: 2045-2322.
Resumo | Links | BibTeX | Tags: Antimicrobials, Molecular engineering in plants
@article{nokey,
title = {MqsR toxin as a biotechnological tool for plant pathogen bacterial control},
author = {Reinaldo Rodrigues Souza-Neto and Isis Gabriela Barbosa Carvalho and Paula Maria Moreira Martins and Simone Cristina Picchi and Juarez Pires Tomaz and Raquel Caserta and Marco Aurélio Takita and Alessandra Alves Souza},
url = {https://www.nature.com/articles/s41598-022-06690-x},
doi = {10.1038/s41598-022-06690-x},
issn = {2045-2322},
year = {2022},
date = {2022-01-01},
journal = {Scientific Reports 2022 12:1},
volume = {12},
issue = {1},
pages = {1-12},
publisher = {Nature Publishing Group},
abstract = {Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.},
keywords = {Antimicrobials, Molecular engineering in plants},
pubstate = {published},
tppubtype = {article}
}
Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.
Souza-Neto, Reinaldo Rodrigues; Carvalho, Isis Gabriela Barbosa; Martins, Paula Maria Moreira; Picchi, Simone Cristina; Tomaz, Juarez Pires; Caserta, Raquel; Takita, Marco Aurélio; Souza, Alessandra Alves
MqsR toxin as a biotechnological tool for plant pathogen bacterial control Journal Article
Em: Scientific Reports 2022 12:1, vol. 12, iss. 1, pp. 1-12, 2022, ISSN: 2045-2322.
Resumo | Links | BibTeX | Tags: Antimicrobials, Molecular engineering in plants
@article{nokey,
title = {MqsR toxin as a biotechnological tool for plant pathogen bacterial control},
author = {Reinaldo Rodrigues Souza-Neto and Isis Gabriela Barbosa Carvalho and Paula Maria Moreira Martins and Simone Cristina Picchi and Juarez Pires Tomaz and Raquel Caserta and Marco Aurélio Takita and Alessandra Alves Souza},
url = {https://www.nature.com/articles/s41598-022-06690-x},
doi = {10.1038/s41598-022-06690-x},
issn = {2045-2322},
year = {2022},
date = {2022-01-01},
journal = {Scientific Reports 2022 12:1},
volume = {12},
issue = {1},
pages = {1-12},
publisher = {Nature Publishing Group},
abstract = {Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.},
keywords = {Antimicrobials, Molecular engineering in plants},
pubstate = {published},
tppubtype = {article}
}
Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.
Vieira, Gabrielle; Khalil, Zeinab G.; Capon, Robert J.; Sette, Lara D.; Ferreira, Henrique; Sass, Daiane C.
Isolation and agricultural potential of penicillic acid against citrus canker Journal Article
Em: Journal of Applied Microbiology, vol. 132, iss. 4, pp. 3081-3088, 2022, ISSN: 1365-2672.
Resumo | Links | BibTeX | Tags: agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases
@article{Vieira2022,
title = {Isolation and agricultural potential of penicillic acid against citrus canker},
author = {Gabrielle Vieira and Zeinab G. Khalil and Robert J. Capon and Lara D. Sette and Henrique Ferreira and Daiane C. Sass},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15413 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15413 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15413},
doi = {10.1111/JAM.15413},
issn = {1365-2672},
year = {2022},
date = {2022-01-01},
journal = {Journal of Applied Microbiology},
volume = {132},
issue = {4},
pages = {3081-3088},
publisher = {John Wiley & Sons, Ltd},
abstract = {Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.},
keywords = {agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases},
pubstate = {published},
tppubtype = {article}
}
Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.