Publicações

@article{nokey,

title = {Drought tolerance memory transmission by citrus buds},

author = {Andressa Rodrigues Oliveira Sousa and Rogério Ferreira Ribas and Mauricio Antônio Coelho Filho and Luciano Freschi and Claudia Fortes Ferreira and Walter Santos Soares Filho and Junior Pastor Pérez-Molina and Abelmon Silva Gesteira},

doi = {10.1016/J.PLANTSCI.2022.111292},

issn = {0168-9452},

year  = {2022},

date = {2022-01-01},

journal = {Plant Science},

volume = {320},

pages = {111292},

publisher = {Elsevier},

abstract = {Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.},

keywords = {Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Drought tolerance memory transmission by citrus buds},

author = {Andressa Rodrigues Oliveira Sousa and Rogério Ferreira Ribas and Mauricio Antônio Coelho Filho and Luciano Freschi and Claudia Fortes Ferreira and Walter Santos Soares Filho and Junior Pastor Pérez-Molina and Abelmon Silva Gesteira},

doi = {10.1016/J.PLANTSCI.2022.111292},

issn = {0168-9452},

year  = {2022},

date = {2022-01-01},

journal = {Plant Science},

volume = {320},

pages = {111292},

publisher = {Elsevier},

abstract = {Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.},

keywords = {Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq},

author = {Luana P. Gonçalves and Raquel L. Boscariol Camargo and Marco Aurélio Takita and Marcos A. Machado and Walter S. Dos Soares Filho and Marcio G. C. Costa},

url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5481-z},

doi = {10.1186/S12864-019-5481-Z/FIGURES/3},

issn = {14712164},

year  = {2019},

date = {2019-01-01},

journal = {BMC Genomics},

volume = {20},

issue = {1},

pages = {1-14},

publisher = {BioMed Central Ltd.},

abstract = {Background: Citrus plants are commercially propagated by grafting, with the rootstock variety influencing a number of horticultural traits, including drought tolerance. Among the different rootstock varieties available for citrus propagation, 'Rangpur' lime is known to confer enhanced tolerance to drought as compared to other citrus rootstocks. The objective of this study was to investigate the poorly understood molecular responses underlying the rootstock-induced drought tolerance in sweet orange. Results: RNA-Seq transcriptome analysis was carried out in leaves of sweet orange grafted on 'Rangpur' lime subjected to control and drought-stress treatments, under greenhouse conditions, using the Illumina HiSeq platform. A total of 41,827 unique transcripts were identified, among which 1764 transcripts showed significant variation (P ≤ 0.001) between the treatments, with 1081 genes induced and 683 repressed by drought-stress treatment. The transcripts were distributed in 44 different categories of cellular component, molecular function and biological process. Several genes related to cell metabolism, including those involved in the metabolisms of cell wall, carbohydrates and antioxidants, light reactions, biotic and abiotic stress responses, as well as genes coding for transcription factors (TFs), protein kinases (PKs) and proteins involved in the abscisic acid (ABA) and ethylene signaling pathways, were differentially regulated by drought stress. RNA-Seq data were validated by quantitative real-time PCR (qPCR) analysis and comparative analysis of expression of the selected genes between sweet orange grafted on drought-tolerant and -sensitive rootstocks revealed new candidate genes for drought tolerance in citrus. Conclusions: In conclusion, our results showed that only a relatively small but functionally diverse fraction of the sweet orange transcriptome, with functions in metabolism, cellular responses and regulation, was differentially regulated by drought stress. The data suggest that the rootstock-induced drought tolerance in sweet orange includes the transcriptional activation of genes related to the cell wall, soluble carbohydrate and antioxidant metabolisms, biotic and abiotic stress responses, TFs, PKs and ABA signaling pathway, and the downregulation of genes involved in the starch metabolism, light reactions and ethylene signaling. Future efforts to elucidate their functional roles and explore their potential in the citrus genetic improvement should benefit from this data.},

keywords = {Abiotic stress, Citrus, Transcriptome, water deficit},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Rootstock-induced molecular responses associated with drought tolerance in sweet orange as revealed by RNA-Seq},

author = {Luana P. Gonçalves and Raquel L. Boscariol Camargo and Marco Aurélio Takita and Marcos A. Machado and Walter S. Dos Soares Filho and Marcio G. C. Costa},

url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5481-z},

doi = {10.1186/S12864-019-5481-Z/FIGURES/3},

issn = {14712164},

year  = {2019},

date = {2019-01-01},

journal = {BMC Genomics},

volume = {20},

issue = {1},

pages = {1-14},

publisher = {BioMed Central Ltd.},

abstract = {Background: Citrus plants are commercially propagated by grafting, with the rootstock variety influencing a number of horticultural traits, including drought tolerance. Among the different rootstock varieties available for citrus propagation, 'Rangpur' lime is known to confer enhanced tolerance to drought as compared to other citrus rootstocks. The objective of this study was to investigate the poorly understood molecular responses underlying the rootstock-induced drought tolerance in sweet orange. Results: RNA-Seq transcriptome analysis was carried out in leaves of sweet orange grafted on 'Rangpur' lime subjected to control and drought-stress treatments, under greenhouse conditions, using the Illumina HiSeq platform. A total of 41,827 unique transcripts were identified, among which 1764 transcripts showed significant variation (P ≤ 0.001) between the treatments, with 1081 genes induced and 683 repressed by drought-stress treatment. The transcripts were distributed in 44 different categories of cellular component, molecular function and biological process. Several genes related to cell metabolism, including those involved in the metabolisms of cell wall, carbohydrates and antioxidants, light reactions, biotic and abiotic stress responses, as well as genes coding for transcription factors (TFs), protein kinases (PKs) and proteins involved in the abscisic acid (ABA) and ethylene signaling pathways, were differentially regulated by drought stress. RNA-Seq data were validated by quantitative real-time PCR (qPCR) analysis and comparative analysis of expression of the selected genes between sweet orange grafted on drought-tolerant and -sensitive rootstocks revealed new candidate genes for drought tolerance in citrus. Conclusions: In conclusion, our results showed that only a relatively small but functionally diverse fraction of the sweet orange transcriptome, with functions in metabolism, cellular responses and regulation, was differentially regulated by drought stress. The data suggest that the rootstock-induced drought tolerance in sweet orange includes the transcriptional activation of genes related to the cell wall, soluble carbohydrate and antioxidant metabolisms, biotic and abiotic stress responses, TFs, PKs and ABA signaling pathway, and the downregulation of genes involved in the starch metabolism, light reactions and ethylene signaling. Future efforts to elucidate their functional roles and explore their potential in the citrus genetic improvement should benefit from this data.},

keywords = {Abiotic stress, Citrus, Transcriptome, water deficit},

pubstate = {published},

tppubtype = {article}

}