Publicações

@article{Goulin2019,

title = {In vitro symptom induction of Colletotrichum abscissum infection in detached sweet orange flowers},

author = {Eduardo Henrique Goulin and Paulo José Camargo Santos and Ronaldo Durigan Dalio and Marcos Antonio Machado},

url = {https://link.springer.com/article/10.1007/s42161-018-00220-3},

doi = {10.1007/S42161-018-00220-3/METRICS},

issn = {22397264},

year  = {2019},

date = {2019-01-01},

journal = {Journal of Plant Pathology},

volume = {101},

issue = {3},

pages = {695-699},

publisher = {Springer},

abstract = {The citrus industry is economically important in several countries around the world, and its primary challenges include pests and diseases, especially in Brazil. Post-bloom fruit drop (PFD), caused by Colletotrichum abscissum, a fungus that belongs to the C. acutatum species complex, is one of the most severe citrus crop diseases, causing damage to blossoms and interfering in the fruit viability, consequently contributing to early drop of the fruit. Little is known about the pathogenic mechanisms of C. abscissum in the flowers of citrus species, so finding an experimental model to study symptom development and gene function under controlled conditions is essential. However, the complexity of the PFD pathosystem in the field does not allow adequate experimental control, and due to regulations, evaluating genetically modified fungi under natural environmental conditions requires prior approval. Studies of gene function can be performed by knockdown, knockout, or deletion in C. abscissum mutants with a focus on symptom development in flowers. We developed a reliable in vitro system as an alternative for symptom induction of C. abscissum infection in detached citrus flowers. Inoculated citrus flowers were placed on Petri dishes with a water-agar substrate and kept in BOD chambers for 72 h. All C. abscissum inoculations resulted in the typical symptoms of PFD, proving the effectiveness of this large-scale screening system, which is fast and reliable and requires less space than that required for greenhouse or open-field experiments.},

keywords = {Citrus disease, Colletotrichum acutatum, Post-bloom fruit drop (PFD), Symptom induction},

pubstate = {published},

tppubtype = {article}

}

@article{Matsumura2019,

title = {Rescue of Citrus sudden death-associated virus in Nicotiana benthamiana plants from cloned cDNA: insights into mechanisms of expression of the three capsid proteins},

author = {Emilyn E. Matsumura and Helvécio D. Coletta-Filho and Marcos A. Machado and Shahideh Nouri and Bryce W. Falk},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12780 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12780 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12780},

doi = {10.1111/MPP.12780},

issn = {1364-3703},

year  = {2019},

date = {2019-01-01},

journal = {Molecular Plant Pathology},

volume = {20},

issue = {5},

pages = {611-625},

publisher = {John Wiley & Sons, Ltd},

abstract = {Citrus sudden death-associated virus (CSDaV) is a member of the genus Marafivirus in the family Tymoviridae, and has been associated with citrus sudden death (CSD) disease in Brazil. Difficulties in the purification of CSDaV from infected citrus plants have prevented progress in the investigation of the role of this virus in CSD and an understanding of its molecular biology. In this work, we have constructed a full-length cDNA clone of CSDaV driven by the 35S promoter (35SRbz-CSDaV). Agrobacterium tumefaciens-mediated inoculation of 35SRbz-CSDaV in Nicotiana benthamiana plants enabled a fast recovery of large amounts of virions from the agroinfiltrated leaves, which allowed a better molecular characterization of CSDaV. In vivo analyses of mutant versions of 35SRbz-CSDaV revealed the expression strategies used by CSDaV for production of the capsid proteins (CPs). We showed that CSDaV virions contain three forms of CP, each of which is generated from the same coding sequence, but by different mechanisms. The major CPp21 is a product of direct translation by leaky scanning from the second start codon in the subgenomic RNA (sgRNA), whereas the minor CPs, p25 and p23, are produced by direct translation from the first start codon in the sgRNA and by trans-proteolytic cleavage processing derived from the p25 precursor, respectively. Together, these findings contribute to advance our understanding of CSDaV genome expression strategies. In addition, the construction and characterization of the CSDaV infectious clone represent important steps towards the investigation of the role of this virus in CSD and of its use as a tool for citrus biotechnology.},

keywords = {associated virus, capsid protein expression, Citrus disease, citrus sudden death, infectious clone, Marafivirus, Tymoviridae},

pubstate = {published},

tppubtype = {article}

}