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2021
Caccalano, M. N.; Dilarri, G.; Zamuner, C. F. C.; Domingues, D. S.; Ferreira, H.
Hexanoic acid: a new potential substitute for copper-based agrochemicals against citrus canker Journal Article
Em: Journal of Applied Microbiology, vol. 131, iss. 5, pp. 2488-2499, 2021, ISSN: 1365-2672.
Resumo | Links | BibTeX | Tags: cell division, Disease management, membrane permeabilization, organic acid, phytotoxicity, Xanthomonas citri
@article{Caccalano2021,
title = {Hexanoic acid: a new potential substitute for copper-based agrochemicals against citrus canker},
author = {M. N. Caccalano and G. Dilarri and C. F. C. Zamuner and D. S. Domingues and H. Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15125 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15125 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15125},
doi = {10.1111/JAM.15125},
issn = {1365-2672},
year = {2021},
date = {2021-01-01},
journal = {Journal of Applied Microbiology},
volume = {131},
issue = {5},
pages = {2488-2499},
publisher = {John Wiley & Sons, Ltd},
abstract = {Aims: The aim of the study is to evaluate hexanoic acid (HA) as an alternative to manage citrus canker. Methods and Results: The minimal growth inhibitory concentration of HA against Xanthomonas citri subsp. citri was determined at 2·15 mmol l−1 using a respiratory activity assay. Growth curves at different pH values showed that growth inhibition was not due to media acidification induced by HA. The germination rate and root elongation of Lactuca sativa seeds exposed to different concentrations of HA (varying from 0·86 to 5·16 mmol l−1) were assessed to screen for phytotoxicity. The acid exhibited low phytotoxicity for L. sativa at 1·29 and 2·58 mmol l−1. To evaluate the ability of HA to protect citrus against X. citri infection, leaves of Citrus sinensis were sprayed with the acid and subsequently challenged with X. citri. HA at 3·44 mmol l−1 was able to protect citrus against infection, showing a reduction of three orders of magnitude in the number of citrus canker lesions per cm2 when compared to the untreated negative control. Conclusion: HA is a potential alternative to copper for citrus canker management. Significance and Impact of the Study: HA inhibits X. citri growth, exhibits low phytotoxicity and is an alternative to copper for the protection of citrus plants against bacterial infection.},
keywords = {cell division, Disease management, membrane permeabilization, organic acid, phytotoxicity, Xanthomonas citri},
pubstate = {published},
tppubtype = {article}
}
Aims: The aim of the study is to evaluate hexanoic acid (HA) as an alternative to manage citrus canker. Methods and Results: The minimal growth inhibitory concentration of HA against Xanthomonas citri subsp. citri was determined at 2·15 mmol l−1 using a respiratory activity assay. Growth curves at different pH values showed that growth inhibition was not due to media acidification induced by HA. The germination rate and root elongation of Lactuca sativa seeds exposed to different concentrations of HA (varying from 0·86 to 5·16 mmol l−1) were assessed to screen for phytotoxicity. The acid exhibited low phytotoxicity for L. sativa at 1·29 and 2·58 mmol l−1. To evaluate the ability of HA to protect citrus against X. citri infection, leaves of Citrus sinensis were sprayed with the acid and subsequently challenged with X. citri. HA at 3·44 mmol l−1 was able to protect citrus against infection, showing a reduction of three orders of magnitude in the number of citrus canker lesions per cm2 when compared to the untreated negative control. Conclusion: HA is a potential alternative to copper for citrus canker management. Significance and Impact of the Study: HA inhibits X. citri growth, exhibits low phytotoxicity and is an alternative to copper for the protection of citrus plants against bacterial infection.
2019
Morão, Luana G.; Polaquini, Carlos R.; Kopacz, Malgorzata; Torrezan, Guilherme S.; Ayusso, Gabriela M.; Dilarri, Guilherme; Cavalca, Lúcia B.; Zielińska, Aleksandra; Scheffers, Dirk Jan; Regasini, Luis O.; Ferreira, Henrique
A simplified curcumin targets the membrane of Bacillus subtilis Journal Article
Em: MicrobiologyOpen, vol. 8, iss. 4, pp. e00683, 2019, ISSN: 2045-8827.
Resumo | Links | BibTeX | Tags: antibacterial compound, cell division, membrane permeabilization, turmeric
@article{nokey,
title = {A simplified curcumin targets the membrane of Bacillus subtilis},
author = {Luana G. Morão and Carlos R. Polaquini and Malgorzata Kopacz and Guilherme S. Torrezan and Gabriela M. Ayusso and Guilherme Dilarri and Lúcia B. Cavalca and Aleksandra Zielińska and Dirk Jan Scheffers and Luis O. Regasini and Henrique Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/abs/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/10.1002/mbo3.683},
doi = {10.1002/MBO3.683},
issn = {2045-8827},
year = {2019},
date = {2019-01-01},
journal = {MicrobiologyOpen},
volume = {8},
issue = {4},
pages = {e00683},
publisher = {John Wiley & Sons, Ltd},
abstract = {Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.},
keywords = {antibacterial compound, cell division, membrane permeabilization, turmeric},
pubstate = {published},
tppubtype = {article}
}
Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.
Morão, Luana G.; Polaquini, Carlos R.; Kopacz, Malgorzata; Torrezan, Guilherme S.; Ayusso, Gabriela M.; Dilarri, Guilherme; Cavalca, Lúcia B.; Zielińska, Aleksandra; Scheffers, Dirk Jan; Regasini, Luis O.; Ferreira, Henrique
A simplified curcumin targets the membrane of Bacillus subtilis Journal Article
Em: MicrobiologyOpen, vol. 8, iss. 4, pp. e00683, 2019, ISSN: 2045-8827.
Resumo | Links | BibTeX | Tags: antibacterial compound, cell division, membrane permeabilization, turmeric
@article{nokey,
title = {A simplified curcumin targets the membrane of Bacillus subtilis},
author = {Luana G. Morão and Carlos R. Polaquini and Malgorzata Kopacz and Guilherme S. Torrezan and Gabriela M. Ayusso and Guilherme Dilarri and Lúcia B. Cavalca and Aleksandra Zielińska and Dirk Jan Scheffers and Luis O. Regasini and Henrique Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/abs/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/10.1002/mbo3.683},
doi = {10.1002/MBO3.683},
issn = {2045-8827},
year = {2019},
date = {2019-01-01},
journal = {MicrobiologyOpen},
volume = {8},
issue = {4},
pages = {e00683},
publisher = {John Wiley & Sons, Ltd},
abstract = {Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.},
keywords = {antibacterial compound, cell division, membrane permeabilization, turmeric},
pubstate = {published},
tppubtype = {article}
}
Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.