Publicações

@article{Caccalano2021,

title = {Hexanoic acid: a new potential substitute for copper-based agrochemicals against citrus canker},

author = {M. N. Caccalano and G. Dilarri and C. F. C. Zamuner and D. S. Domingues and H. Ferreira},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15125 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15125 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15125},

doi = {10.1111/JAM.15125},

issn = {1365-2672},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Applied Microbiology},

volume = {131},

issue = {5},

pages = {2488-2499},

publisher = {John Wiley & Sons, Ltd},

abstract = {Aims: The aim of the study is to evaluate hexanoic acid (HA) as an alternative to manage citrus canker. Methods and Results: The minimal growth inhibitory concentration of HA against Xanthomonas citri subsp. citri was determined at 2·15 mmol l−1 using a respiratory activity assay. Growth curves at different pH values showed that growth inhibition was not due to media acidification induced by HA. The germination rate and root elongation of Lactuca sativa seeds exposed to different concentrations of HA (varying from 0·86 to 5·16 mmol l−1) were assessed to screen for phytotoxicity. The acid exhibited low phytotoxicity for L. sativa at 1·29 and 2·58 mmol l−1. To evaluate the ability of HA to protect citrus against X. citri infection, leaves of Citrus sinensis were sprayed with the acid and subsequently challenged with X. citri. HA at 3·44 mmol l−1 was able to protect citrus against infection, showing a reduction of three orders of magnitude in the number of citrus canker lesions per cm2 when compared to the untreated negative control. Conclusion: HA is a potential alternative to copper for citrus canker management. Significance and Impact of the Study: HA inhibits X. citri growth, exhibits low phytotoxicity and is an alternative to copper for the protection of citrus plants against bacterial infection.},

keywords = {cell division, Disease management, membrane permeabilization, organic acid, phytotoxicity, Xanthomonas citri},

pubstate = {published},

tppubtype = {article}

}

@article{Mercaldi2021,

title = {Molecular basis for diaryldiamine selectivity and competition with tRNA in a type 2 methionyl-tRNA synthetase from a gram-negative bacterium},

author = {Gustavo Fernando Mercaldi and Maxuel Oliveira Andrade and Jackeline Lima Zanella and Artur Torres Cordeiro and Celso Eduardo Benedetti},

url = {http://www.jbc.org/article/S0021925821004464/fulltext http://www.jbc.org/article/S0021925821004464/abstract https://www.jbc.org/article/S0021-9258(21)00446-4/abstract},

doi = {10.1016/j.jbc.2021.100658},

issn = {1083351X},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Biological Chemistry},

volume = {296},

publisher = {American Society for Biochemistry and Molecular Biology Inc.},

abstract = {Gram-negative bacteria are responsible for a variety of human, animal, and plant diseases. The spread of multidrug-resistant Gram-negative bacteria poses a challenge to disease control and highlights the need for novel antimicrobials. Owing to their critical role in protein synthesis, aminoacyl-tRNA synthetases, including the methionyl-tRNA synthetases MetRS1 and MetRS2, are attractive drug targets. MetRS1 has long been exploited as a drug target in Gram-positive bacteria and protozoan parasites. However, MetRS1 inhibitors have limited action upon Gram-negative pathogens or on Gram-positive bacteria that produce MetRS2 enzymes. The underlying mechanism by which MetRS2 enzymes are insensitive to MetRS1 inhibitors is presently unknown. Herein, we report the first structures of MetRS2 from a multidrug-resistant Gram-negative bacterium in its ligand-free state and bound to its substrate or MetRS1 inhibitors. The structures reveal the binding mode of two diaryldiamine MetRS1 inhibitors that occupy the amino acid–binding site and a surrounding auxiliary pocket implicated in tRNA acceptor arm binding. The structural features associated with amino acid polymorphisms found in the methionine and auxiliary pockets reveal the molecular basis for diaryldiamine binding and selectivity between MetRS1 and MetRS2 enzymes. Moreover, we show that mutations in key polymorphic residues in the methionine and auxiliary pockets not only altered inhibitor binding affinity but also significantly reduced enzyme function. Our findings thus reinforce the tRNA acceptor arm binding site as a druggable pocket in class I aminoacyl-tRNA synthetases and provide a structural basis for optimization of MetRS2 inhibitors for the development of new antimicrobials against Gram-negative pathogens.},

keywords = {A76, AaMetRS, AaRS, adenosine 76, aminoacyl-tRNA synthetase, AMR, antimicrobial resistance, Aquifex aeolicus MetRS, Bederocin, Brazilian Bioscience National Laboratory/Brazilian Centre for Research in Energy and Materials, Campylobacter jejuni Inosine-5'-monophosphate dehydrogenase, CjIMPDH, connective polypeptide, CP, diaryldiamines, differential scanning fluorimetry, dimethyl sulfoxide, DMSO, DSF, E. coli Leucyl-tRNA synthetase, EcLeuRS, EcMetRS, Escherichia coli MetRS, isothermal titration calorimetry, ITC, L-Met, L-methionine, LNBio/CNPEM, melting temperature, methionine-tRNA synthetase, MetRS, PDB, Protein Data Bank, REP3123, REP8839, Saccharomyces cerevisiae AMP deaminase, ScAMD, Tm, truncated version of XcMetRS lacking the C-terminal extension domain, Xanthomonas citri, Xanthomonas citri MetRS, XcMetRS, XcΔMetRS},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The copper-inducible copAB operon in Xanthomonas citri subsp. citri is regulated at transcriptional and translational levels},

author = {Eliane Cristina De Freitas and Amanda Piovesan Ucci and Elaine Cristina Teixeira and Gisele Audrei Pedroso and Eduardo Hilario and Vitória Fernanda Bertolazzi Zocca and Gabriela Barbosa De Paiva and Henrique Ferreira and Danielle Biscaro Pedrolli and Maria Célia Bertolini},

url = {https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000767},

doi = {10.1099/MIC.0.000767/CITE/REFWORKS},

issn = {14652080},

year  = {2019},

date = {2019-01-01},

journal = {Microbiology (United Kingdom)},

volume = {165},

issue = {3},

pages = {355-365},

publisher = {Microbiology Society},

abstract = {Upstream open reading frames (ORFs) are frequently found in the 5¢-flanking regions of genes and may have a regulatory role in gene expression. A small ORF (named cohL here) was identified upstream from the copAB copper operon in Xanthomonascitri subsp. citri (Xac). We previously demonstrated that copAB expression was induced by copper and that gene inactivation produced a mutant strain that was unable to grow in the presence of copper. Here, we address the role of cohL in copAB expression control. We demonstrate that cohL expression is induced by copper in a copAB-independent manner. Although cohL is transcribed, the CohL protein is either not expressed in vivo or is synthesized at undetectable levels. Inactivation of cohL (X. citri cohL polar mutant strain) leads to an inability to synthesize cohL and copAB transcripts and consequently the inability to grow in the presence of copper. Bioinformatic tools predicted a stem-loop structure for the cohL-copAB intergenic region and revealed that this region may arrange itself in a secondary structure. Using in vitro gene expression, we found out that the structured 5¢-UTR mRNA of copAB is responsible for sequestering the ribosome-binding site that drives the translation of copA. However, copper alone was not able to release the sequence. Based on the results, we speculate that cohL plays a role as a regulatory RNA rather than as a protein-coding gene.},

keywords = {Copper resistance, Gene expression, Short upstream ORF, Xanthomonas citri},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The copper-inducible copAB operon in Xanthomonas citri subsp. citri is regulated at transcriptional and translational levels},

author = {Eliane Cristina De Freitas and Amanda Piovesan Ucci and Elaine Cristina Teixeira and Gisele Audrei Pedroso and Eduardo Hilario and Vitória Fernanda Bertolazzi Zocca and Gabriela Barbosa De Paiva and Henrique Ferreira and Danielle Biscaro Pedrolli and Maria Célia Bertolini},

url = {https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000767},

doi = {10.1099/MIC.0.000767/CITE/REFWORKS},

issn = {14652080},

year  = {2019},

date = {2019-01-01},

journal = {Microbiology (United Kingdom)},

volume = {165},

issue = {3},

pages = {355-365},

publisher = {Microbiology Society},

abstract = {Upstream open reading frames (ORFs) are frequently found in the 5¢-flanking regions of genes and may have a regulatory role in gene expression. A small ORF (named cohL here) was identified upstream from the copAB copper operon in Xanthomonascitri subsp. citri (Xac). We previously demonstrated that copAB expression was induced by copper and that gene inactivation produced a mutant strain that was unable to grow in the presence of copper. Here, we address the role of cohL in copAB expression control. We demonstrate that cohL expression is induced by copper in a copAB-independent manner. Although cohL is transcribed, the CohL protein is either not expressed in vivo or is synthesized at undetectable levels. Inactivation of cohL (X. citri cohL polar mutant strain) leads to an inability to synthesize cohL and copAB transcripts and consequently the inability to grow in the presence of copper. Bioinformatic tools predicted a stem-loop structure for the cohL-copAB intergenic region and revealed that this region may arrange itself in a secondary structure. Using in vitro gene expression, we found out that the structured 5¢-UTR mRNA of copAB is responsible for sequestering the ribosome-binding site that drives the translation of copA. However, copper alone was not able to release the sequence. Based on the results, we speculate that cohL plays a role as a regulatory RNA rather than as a protein-coding gene.},

keywords = {Copper resistance, Gene expression, Short upstream ORF, Xanthomonas citri},

pubstate = {published},

tppubtype = {article}

}

@article{Shimo2019,

title = {Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance},

author = {Hugo Massayoshi Shimo and Carolina Terassi and Caio Cesar Lima Silva and Jackeline Lima Zanella and Gustavo Fernando Mercaldi and Silvana Aparecida Rocco and Celso Eduardo Benedetti},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12815 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12815 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12815},

doi = {10.1111/MPP.12815},

issn = {1364-3703},

year  = {2019},

date = {2019-01-01},

journal = {Molecular Plant Pathology},

volume = {20},

issue = {8},

pages = {1105-1118},

publisher = {John Wiley & Sons, Ltd},

abstract = {Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unknown. Here, we show that the Citrus sinensis CAF1 (CsCAF1) is a magnesium-dependent deadenylase implicated in resistance against the citrus canker bacteria Xanthomonas citri. CsCAF1 interacted with proteins of the CCR4-NOT complex, including CsVIP2, a NOT2 homologue, translin-associated factor X (CsTRAX) and the poly(A)-binding proteins CsPABPN and CsPABPC. CsCAF1 also interacted with PthA4, the main X. citri effector required for citrus canker elicitation. We also present evidence suggesting that PthA4 inhibits CsCAF1 deadenylase activity in vitro and stabilizes the mRNA encoded by the citrus canker susceptibility gene CsLOB1, which is transcriptionally activated by PthA4 during canker formation. Moreover, we show that an inhibitor of CsCAF1 deadenylase activity significantly enhanced canker development, despite causing a reduction in PthA4-dependent CsLOB1 transcription. These results thus link CsCAF1 with canker development and PthA4-dependent transcription in citrus plants.},

keywords = {associated factor, CCR4, Citrus canker, Citrus sinensis, CsCAF1, CsLOB1, NOT, PthA4, RNA deadenylase activity, TAL effectors, Xanthomonas aurantifolii, Xanthomonas citri},

pubstate = {published},

tppubtype = {article}

}

@article{Lorenzoni2017,

title = {Xanthomonas citri MinC oscillates from pole to pole to ensure proper cell division and shape},

author = {André S. G. Lorenzoni and Giordanni C. Dantas and Tessa Bergsma and Henrique Ferreira and Dirk Jan Scheffers},

doi = {10.3389/FMICB.2017.01352/BIBTEX},

issn = {1664302X},

year  = {2017},

date = {2017-01-01},

journal = {Frontiers in Microbiology},

volume = {8},

issue = {JUL},

pages = {1352},

publisher = {Frontiers Media S.A.},

abstract = {Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Δmin phenotype observed in other bacteria deleted for min components: minicells and short filamentation. In addition we noticed the formation of branches, which is similar to what was previously described for Escherichia coli deleted for either min or for several low molecular weight penicillin-binding proteins (PBPs). The branching phenotype was medium dependent and probably linked to gluconeogenic growth. We complemented the minC gene by integrating gfp-minC into the amy locus. Xac complemented strains displayed a wild-type phenotype. In addition, GFP-MinC oscillated from pole to pole, similar to MinCD oscillations observed in E. coli and more recently in Synechococcus elongatus. Further investigation of the branching phenotype revealed that in branching cells nucleoid organization, divisome formation and peptidoglycan incorporation were disrupted.},

keywords = {Citrus canker, FtsZ, MinC, ParB, Peptidoglycan, Xanthomonas citri, ZapA},

pubstate = {published},

tppubtype = {article}

}