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2021
Bezerra, Yane Caroline Dos Anjos; Marques, João Paulo Rodrigues; Stipp, Liliane Cristina Liborio; Attílio, Lísia Borges; Freitas-Astúa, Juliana; Filho, Francisco Assis Alves Mourão
How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters Journal Article
Em: Revista Brasileira de Fruticultura, vol. 43, iss. 4, 2021, ISSN: 0100-2945.
Resumo | Links | BibTeX | Tags: Candidatus Liberibacter spp., disease resistance, genetic transformation, GUS, histolocalization
@article{Bezerra2021,
title = {How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters},
author = {Yane Caroline Dos Anjos Bezerra and João Paulo Rodrigues Marques and Liliane Cristina Liborio Stipp and Lísia Borges Attílio and Juliana Freitas-Astúa and Francisco Assis Alves Mourão Filho},
url = {http://www.scielo.br/j/rbf/a/tg5tQ8p8CcRK7xKDDcHCTjF/?lang=en},
doi = {10.1590/0100-29452021005},
issn = {0100-2945},
year = {2021},
date = {2021-01-01},
journal = {Revista Brasileira de Fruticultura},
volume = {43},
issue = {4},
publisher = {Sociedade Brasileira de Fruticultura},
abstract = {Abstract New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ‘Carrizo’ citrange [ (Poncirus trifoliataL.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (ß-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ‘Rangpur’ lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissue-specific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.},
keywords = {Candidatus Liberibacter spp., disease resistance, genetic transformation, GUS, histolocalization},
pubstate = {published},
tppubtype = {article}
}
Abstract New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ‘Carrizo’ citrange [ (Poncirus trifoliataL.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (ß-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ‘Rangpur’ lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissue-specific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
Pacheco, Camilla Andrade; Moreira, Alécio Souza; Girardi, Eduardo Augusto; Bassanezi, Renato Beozzo; Stuchi, Eduardo Sanches
Tree growth, production and huanglongbing incidence of sweet orange varieties using different nursery tree standards Journal Article
Em: Scientia Horticulturae, vol. 284, pp. 110023, 2021, ISSN: 0304-4238.
Resumo | Links | BibTeX | Tags: Candidatus Liberibacter spp., CITRUS GREENING, Citrus spp., propagation, Resetting, Survival analysis
@article{Pacheco2021,
title = {Tree growth, production and huanglongbing incidence of sweet orange varieties using different nursery tree standards},
author = {Camilla Andrade Pacheco and Alécio Souza Moreira and Eduardo Augusto Girardi and Renato Beozzo Bassanezi and Eduardo Sanches Stuchi},
doi = {10.1016/J.SCIENTA.2021.110023},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {284},
pages = {110023},
publisher = {Elsevier},
abstract = {Insect-proof propagation of citrus is recommended to prevent vector-transmitted pathogens such as Candidatus Liberibacter spp. and Xylella fastidiosa. Although larger nursery trees were related to a superior post-planting performance in other perennial crops, there are few studies on citrus. This fact motivated us to evaluate the tree growth, the fruit production, and the huanglongbing (HLB) incidence of four commercial sweet orange varieties (‘Valencia Late’, ‘Hamlin’, ‘Pera’ and ‘Folha Murcha’) using two nursery tree standards (young whip and large headed trees). Eight years after planting, the tree size was similar between the evaluated treatments, especially for more vigorous varieties. However, large headed trees anticipated and increased the cumulative production by 30 % in average, which can improve the performance of reset trees in particular. Regardless the nursery tree standard and sweet orange variety, the cumulative incidence of HLB was relatively high over eight years. Therefore, a strict control of the vector should be reinforced in the field irrespectively of these factors.},
keywords = {Candidatus Liberibacter spp., CITRUS GREENING, Citrus spp., propagation, Resetting, Survival analysis},
pubstate = {published},
tppubtype = {article}
}
Insect-proof propagation of citrus is recommended to prevent vector-transmitted pathogens such as Candidatus Liberibacter spp. and Xylella fastidiosa. Although larger nursery trees were related to a superior post-planting performance in other perennial crops, there are few studies on citrus. This fact motivated us to evaluate the tree growth, the fruit production, and the huanglongbing (HLB) incidence of four commercial sweet orange varieties (‘Valencia Late’, ‘Hamlin’, ‘Pera’ and ‘Folha Murcha’) using two nursery tree standards (young whip and large headed trees). Eight years after planting, the tree size was similar between the evaluated treatments, especially for more vigorous varieties. However, large headed trees anticipated and increased the cumulative production by 30 % in average, which can improve the performance of reset trees in particular. Regardless the nursery tree standard and sweet orange variety, the cumulative incidence of HLB was relatively high over eight years. Therefore, a strict control of the vector should be reinforced in the field irrespectively of these factors.