Publications

@article{Fujimoto2022,

title = {Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction},

author = {Andréia Fujimoto and Fábio Augusto and Taicia Pacheco Fill and Rafael Kupper Moretto and Katia Cristina Kupper},

url = {https://link.springer.com/article/10.1007/s11274-021-03214-z},

doi = {10.1007/S11274-021-03214-Z/METRICS},

issn = {15730972},

year  = {2022},

date = {2022-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {38},

issue = {3},

pages = {1-14},

publisher = {Springer Science and Business Media B.V.},

abstract = {Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.},

keywords = {biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds},

pubstate = {published},

tppubtype = {article}

}

@article{Cunha2020,

title = {The action of yeast strains as biocontrol agents against Penicillium digitatum in Lima sweet oranges},

author = {Tatiane Cunha and Luriany Pompeo Ferraz and Gilmar Katia Cristina Kupper and Tatiane Cunha and Luriany Pompeo Ferraz and Gilmar Katia Cristina Kupper},

url = {http://citrusrt.ccsm.br/journal/citrusrt/article/doi/10.4322/crt.18819},

doi = {10.4322/CRT.18819},

issn = {21775419},

year  = {2020},

date = {2020-01-01},

journal = {Citrus Research & Technology},

volume = {41},

pages = {1-9},

publisher = {Centro de Citricultura Sylvio Moreira/Instituto Agronômico},

abstract = {The green mold caused by Penicillium digitatum is the most important disease affecting citrus in the postharvest period. An alternative to control the disease lies on the use of yeasts. The purpose of the present study was to assess the ability of 101 yeasts strains to control P. digitatumin vitro and in vivo. The assay followed a randomized design with five repetitions and the evaluation took into consideration the mean pathogen colony diameter in Petri dish paired culture. In addition, the effect of the fungus on yeast strain growth was assessed. Orange fruits (Citrus sinensis cv. Lima) were injured in two equidistant points and inoculated with P. digitatum conidia suspension 24 hours before and after treatments, in the assays conducted in vivo. Treatments consisted in the application of eight yeast suspensions, fungicide and distilled water (control treatment); they followed a randomized design with three repetitions (20 fruits per repetition). The best antagonist results in vitro were recorded for five strains (Saccharomyces cerevisiae ACBL-76 and ACBL-82 strains; Candida stellimalicola ACBL-84 strain; and ACBL-87), which presented mycelial growth inhibition values higher than 80%. Two yeast strains (S. cerevisiae ACBL-82 strain and Meyerozyma caribbica ACBL-86 strain) have successfully controlled the progress and incidence of green mold in orange fruits when they were applied in a preventive manner.},

keywords = {antagonist yeasts, biological control, Citrus sinensis, Index terms: green mold, postharvest},

pubstate = {published},

tppubtype = {article}

}

@article{Nunes2020,

title = {Survey of the citrus leprosis vector (Brevipalpus yothersi) and phytoseiids in spontaneous plants of an organic citrus orchard},

author = {Maria A. Nunes and Valdenice M. Novelli and Bruna A. Cunha and Alex J. Soares and Jeferson L. C. Mineiro and Juliana Freitas-Astúa and Marinês Bastianel},

url = {https://link.springer.com/article/10.1007/s10493-020-00543-w},

doi = {10.1007/S10493-020-00543-W/METRICS},

issn = {15729702},

year  = {2020},

date = {2020-01-01},

journal = {Experimental and Applied Acarology},

volume = {82},

issue = {2},

pages = {199-209},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Citrus leprosis (CL) is one of the most important viral diseases in sweet orange orchards in Latin America. It is caused by members of at least five species of the so-called Brevipalpus-transmitted viruses (BTV), and the prevalent is Citrus leprosis virus C (CiLV-C). This virus has the broadest host range amongst all CL-associated viruses and is transmitted by Brevipalpus yothersi, a polyphagous mite that can colonize a large variety of host plants, including some spontaneous ground cover plants. But if, on one hand, spontaneous plants can host CL virus and vector, on the other hand, they can offer alternative food for predators, equally common in organic citrus orchards. Brevipalpus yothersi and predator mites were surveyed in 33 spontaneous plants of a Westin sweet orange orchard conducted under organic production system in Brazil, from June 2010 to April 2011. Predatory mites were identified as phytoseiids, and Iphiseiodes zuluagai was the prevalent species, representing 58% of all predators. Other phytoseiids were considered accidental species in the area. Ageratum conyzoides and Alternanthera tenella were the most represented plant host species to predators, comprising 28 and 10% of the total surveyed plants, respectively. Brevipalpus yothersi specimens were detected on various spontaneous species: A. conyzoides, A. tenella, Amaranthus deflexus, Bidens pilosa, Ipomoea quamoclit, I. cairica, Merremia cissoides, Solanum americanum, Panicum maximum, and, predominantly, Commelina benghalensis. The latter has been previously reported as host of CiLV-C as well and, therefore, it is recommended to eliminate this species from citrus orchards.},

keywords = {biological control, citrus leprosis virus C, Citrus management, false spider mites, Mite survey},

pubstate = {published},

tppubtype = {article}

}

@article{Bazioli2019,

title = {Biological Control of Citrus Postharvest Phytopathogens},

author = {Jaqueline Moraes Bazioli and João Raul Belinato and Jonas Henrique Costa and Daniel Yuri Akiyama and João Guilherme Moraes Pontes and Katia Cristina Kupper and Fabio Augusto and João Ernesto Carvalho and Taícia Pacheco Fill},

url = {https://www.mdpi.com/2072-6651/11/8/460/htm https://www.mdpi.com/2072-6651/11/8/460},

doi = {10.3390/TOXINS11080460},

issn = {2072-6651},

year  = {2019},

date = {2019-01-01},

journal = {Toxins 2019, Vol. 11, Page 460},

volume = {11},

issue = {8},

pages = {460},

publisher = {Multidisciplinary Digital Publishing Institute},

abstract = {Citrus are vulnerable to the postharvest decay caused by Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii, which are responsible for the green mold, blue mold, and sour rot post-harvest disease, respectively. The widespread economic losses in citriculture caused by these phytopathogens are minimized with the use of synthetic fungicides such as imazalil, thiabendazole, pyrimethanil, and fludioxonil, which are mainly employed as control agents and may have harmful effects on human health and environment. To date, numerous non-chemical postharvest treatments have been investigated for the control of these pathogens. Several studies demonstrated that biological control using microbial antagonists and natural products can be effective in controlling postharvest diseases in citrus, as well as the most used commercial fungicides. Therefore, microbial agents represent a considerably safer and low toxicity alternative to synthetic fungicides. In the present review, these biological control strategies as alternative to the chemical fungicides are summarized here and new challenges regarding the development of shelf-stable formulated biocontrol products are also discussed.},

keywords = {<i>Geothrichum citri, <i>Penicillium digitatum</i>, <i>Penicillium italicum</i>, aurantii</i>, biological control, harvest phytopathogen, post},

pubstate = {published},

tppubtype = {article}

}

@article{Klein2018,

title = {Biofilm production by Aureobasidium pullulans improves biocontrol against sour rot in citrus},

author = {Mariana Nadjara Klein and Katia Cristina Kupper},

doi = {10.1016/J.FM.2017.07.008},

issn = {0740-0020},

year  = {2018},

date = {2018-01-01},

journal = {Food Microbiology},

volume = {69},

pages = {1-10},

publisher = {Academic Press},

abstract = {The biocontrol efficiency of Aureobasidium pullulans strain ACBL-77 against Geotrichum citri-aurantii, causal agent of sour rot in citrus, and their interactions were evaluated. For this, were evaluated the incorporation of nutrients in optimizing the antagonistic activity of the yeast, the competition for nutrients between microorganisms, the effect of nutrients on yeast cell and biofilm production and their correlation in the biocontrol of disease, the survival of yeast in citrus fruits and the interaction between microorganisms using scanning electron microscopy. Micronutrients (boric acid, cobalt chloride and ammonium molybdate) favoured the antagonistic action of A. pullulans. Ammonium sulfate 1% and sucrose 0.5% favoured the yeast during the competition between the microorganisms. The addition of ammonium sulfate (1%) in the yeast culture stimulated biofilm production and increased the antagonistic activity against the disease, as also allowed the better survival of yeast in wounded sites of citrus fruit. The yeast was found to be able to form biofilms on citrus, deforming the pathogen hyphae. These results showed the importance of the addition of nutrients in A. pullulans based-formulations when aiming for their use on a commercial scale. This is the first report of a positive correlation between the increase in the quantity of biofilm produced by A. pullulans, with increased antagonistic activity.},

keywords = {Ammonium sulfate, biological control, Electron microscopy, Geotrichum citri-aurantii},

pubstate = {published},

tppubtype = {article}

}