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Check the list of papers from NScTI Citrus
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2022
Fujimoto, Andréia; Augusto, Fábio; Fill, Taicia Pacheco; Moretto, Rafael Kupper; Kupper, Katia Cristina
Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction Journal Article
In: World Journal of Microbiology and Biotechnology, vol. 38, iss. 3, pp. 1-14, 2022, ISSN: 15730972.
Abstract | Links | BibTeX | Tags: biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds
@article{Fujimoto2022,
title = {Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction},
author = {Andréia Fujimoto and Fábio Augusto and Taicia Pacheco Fill and Rafael Kupper Moretto and Katia Cristina Kupper},
url = {https://link.springer.com/article/10.1007/s11274-021-03214-z},
doi = {10.1007/S11274-021-03214-Z/METRICS},
issn = {15730972},
year = {2022},
date = {2022-01-01},
journal = {World Journal of Microbiology and Biotechnology},
volume = {38},
issue = {3},
pages = {1-14},
publisher = {Springer Science and Business Media B.V.},
abstract = {Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.},
keywords = {biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds},
pubstate = {published},
tppubtype = {article}
}
Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.
2018
Souza, João Raul Belinato; Kupper, Katia Cristina; Augusto, Fabio
In: Microchemical Journal, vol. 141, pp. 204-209, 2018, ISSN: 0026-265X.
Abstract | Links | BibTeX | Tags: Comprehensive two-dimensional gas chromatography, Postharvest diseases, Solid-phase microextraction, Volatile organic compounds
@article{nokey,
title = {In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis},
author = {João Raul Belinato Souza and Katia Cristina Kupper and Fabio Augusto},
doi = {10.1016/J.MICROC.2018.05.036},
issn = {0026-265X},
year = {2018},
date = {2018-01-01},
journal = {Microchemical Journal},
volume = {141},
pages = {204-209},
publisher = {Elsevier},
abstract = {Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC × GC–MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection.},
keywords = {Comprehensive two-dimensional gas chromatography, Postharvest diseases, Solid-phase microextraction, Volatile organic compounds},
pubstate = {published},
tppubtype = {article}
}
Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC × GC–MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection.
Souza, João Raul Belinato; Kupper, Katia Cristina; Augusto, Fabio
In: Microchemical Journal, vol. 141, pp. 204-209, 2018, ISSN: 0026-265X.
Abstract | Links | BibTeX | Tags: Comprehensive two-dimensional gas chromatography, Postharvest diseases, Solid-phase microextraction, Volatile organic compounds
@article{nokey,
title = {In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis},
author = {João Raul Belinato Souza and Katia Cristina Kupper and Fabio Augusto},
doi = {10.1016/J.MICROC.2018.05.036},
issn = {0026-265X},
year = {2018},
date = {2018-01-01},
journal = {Microchemical Journal},
volume = {141},
pages = {204-209},
publisher = {Elsevier},
abstract = {Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC × GC–MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection.},
keywords = {Comprehensive two-dimensional gas chromatography, Postharvest diseases, Solid-phase microextraction, Volatile organic compounds},
pubstate = {published},
tppubtype = {article}
}
Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC × GC–MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection.
Souza, João Raul Belinato; Kupper, Katia Cristina; Augusto, Fabio
In: Microchemical Journal, vol. 141, pp. 204-209, 2018, ISSN: 0026-265X.
Abstract | Links | BibTeX | Tags: Comprehensive two-dimensional gas chromatography, Postharvest diseases, Solid-phase microextraction, Volatile organic compounds
@article{nokey,
title = {In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis},
author = {João Raul Belinato Souza and Katia Cristina Kupper and Fabio Augusto},
doi = {10.1016/J.MICROC.2018.05.036},
issn = {0026-265X},
year = {2018},
date = {2018-01-01},
journal = {Microchemical Journal},
volume = {141},
pages = {204-209},
publisher = {Elsevier},
abstract = {Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC × GC–MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection.},
keywords = {Comprehensive two-dimensional gas chromatography, Postharvest diseases, Solid-phase microextraction, Volatile organic compounds},
pubstate = {published},
tppubtype = {article}
}
Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC × GC–MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection.