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2022
Fujimoto, Andréia; Augusto, Fábio; Fill, Taicia Pacheco; Moretto, Rafael Kupper; Kupper, Katia Cristina
Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction Journal Article
In: World Journal of Microbiology and Biotechnology, vol. 38, iss. 3, pp. 1-14, 2022, ISSN: 15730972.
Abstract | Links | BibTeX | Tags: biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds
@article{Fujimoto2022,
title = {Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction},
author = {Andréia Fujimoto and Fábio Augusto and Taicia Pacheco Fill and Rafael Kupper Moretto and Katia Cristina Kupper},
url = {https://link.springer.com/article/10.1007/s11274-021-03214-z},
doi = {10.1007/S11274-021-03214-Z/METRICS},
issn = {15730972},
year = {2022},
date = {2022-01-01},
journal = {World Journal of Microbiology and Biotechnology},
volume = {38},
issue = {3},
pages = {1-14},
publisher = {Springer Science and Business Media B.V.},
abstract = {Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.},
keywords = {biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds},
pubstate = {published},
tppubtype = {article}
}
Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.
2018
Rodríguez, Ana; Kava, Vanessa; Latorre-García, Lorena; Silva, Geraldo J.; Pereira, Rosana G.; Glienke, Chirlei; Ferreira-Maba, Lisandra S.; Vicent, Antonio; Shimada, Takehiko; Peña, Leandro
In: Molecular Plant Pathology, vol. 19, iss. 9, pp. 2077-2093, 2018, ISSN: 1364-3703.
Abstract | Links | BibTeX | Tags: CBS, d, genetic engineering, Guignardia citricarpa, limonene, linalool, Phyllosticta citricarpa
@article{nokey,
title = {Engineering d-limonene synthase down-regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence},
author = {Ana Rodríguez and Vanessa Kava and Lorena Latorre-García and Geraldo J. Silva and Rosana G. Pereira and Chirlei Glienke and Lisandra S. Ferreira-Maba and Antonio Vicent and Takehiko Shimada and Leandro Peña},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12681 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12681 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12681},
doi = {10.1111/MPP.12681},
issn = {1364-3703},
year = {2018},
date = {2018-01-01},
journal = {Molecular Plant Pathology},
volume = {19},
issue = {9},
pages = {2077-2093},
publisher = {John Wiley & Sons, Ltd},
abstract = {Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20–24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.},
keywords = {CBS, d, genetic engineering, Guignardia citricarpa, limonene, linalool, Phyllosticta citricarpa},
pubstate = {published},
tppubtype = {article}
}
Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20–24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.
Rodríguez, Ana; Kava, Vanessa; Latorre-García, Lorena; Silva, Geraldo J.; Pereira, Rosana G.; Glienke, Chirlei; Ferreira-Maba, Lisandra S.; Vicent, Antonio; Shimada, Takehiko; Peña, Leandro
In: Molecular Plant Pathology, vol. 19, iss. 9, pp. 2077-2093, 2018, ISSN: 1364-3703.
Abstract | Links | BibTeX | Tags: CBS, d, genetic engineering, Guignardia citricarpa, limonene, linalool, Phyllosticta citricarpa
@article{nokey,
title = {Engineering d-limonene synthase down-regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence},
author = {Ana Rodríguez and Vanessa Kava and Lorena Latorre-García and Geraldo J. Silva and Rosana G. Pereira and Chirlei Glienke and Lisandra S. Ferreira-Maba and Antonio Vicent and Takehiko Shimada and Leandro Peña},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12681 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12681 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12681},
doi = {10.1111/MPP.12681},
issn = {1364-3703},
year = {2018},
date = {2018-01-01},
journal = {Molecular Plant Pathology},
volume = {19},
issue = {9},
pages = {2077-2093},
publisher = {John Wiley & Sons, Ltd},
abstract = {Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20–24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.},
keywords = {CBS, d, genetic engineering, Guignardia citricarpa, limonene, linalool, Phyllosticta citricarpa},
pubstate = {published},
tppubtype = {article}
}
Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20–24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.
Rodríguez, Ana; Kava, Vanessa; Latorre-García, Lorena; Silva, Geraldo J.; Pereira, Rosana G.; Glienke, Chirlei; Ferreira-Maba, Lisandra S.; Vicent, Antonio; Shimada, Takehiko; Peña, Leandro
In: Molecular Plant Pathology, vol. 19, iss. 9, pp. 2077-2093, 2018, ISSN: 1364-3703.
Abstract | Links | BibTeX | Tags: CBS, d, genetic engineering, Guignardia citricarpa, limonene, linalool, Phyllosticta citricarpa
@article{nokey,
title = {Engineering d-limonene synthase down-regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence},
author = {Ana Rodríguez and Vanessa Kava and Lorena Latorre-García and Geraldo J. Silva and Rosana G. Pereira and Chirlei Glienke and Lisandra S. Ferreira-Maba and Antonio Vicent and Takehiko Shimada and Leandro Peña},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12681 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12681 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12681},
doi = {10.1111/MPP.12681},
issn = {1364-3703},
year = {2018},
date = {2018-01-01},
journal = {Molecular Plant Pathology},
volume = {19},
issue = {9},
pages = {2077-2093},
publisher = {John Wiley & Sons, Ltd},
abstract = {Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20–24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.},
keywords = {CBS, d, genetic engineering, Guignardia citricarpa, limonene, linalool, Phyllosticta citricarpa},
pubstate = {published},
tppubtype = {article}
}
Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20–24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.