Publications

@article{Fujimoto2022,

title = {Biocontrol of Phyllosticta citricarpa by Bacillus spp.: biological and chemical aspects of the microbial interaction},

author = {Andréia Fujimoto and Fábio Augusto and Taicia Pacheco Fill and Rafael Kupper Moretto and Katia Cristina Kupper},

url = {https://link.springer.com/article/10.1007/s11274-021-03214-z},

doi = {10.1007/S11274-021-03214-Z/METRICS},

issn = {15730972},

year  = {2022},

date = {2022-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {38},

issue = {3},

pages = {1-14},

publisher = {Springer Science and Business Media B.V.},

abstract = {Citrus fruits are the most produced fruits in the world, but they are threatened by several pathogens, including the fungus Phyllosticta citricarpa, the causal agent of citrus black spot (CBS). The fungus affects most citrus species and the infection results in economic losses in citrus-producing areas. This disease causes the aesthetic depreciation of fresh fruit, impairing its commercialization. As an alternative to the use of synthetic fungicides to control the pathogen, the biological control, using bacteria of the genus Bacillus, is highlighted. Such microorganisms enable biocontrol by the production of volatile organic compounds (VOC) or non-volatile. Therefore, this work aimed to evaluate the production of VOC by isolates of Bacillus spp. grown in different culture media; to evaluate the effects of these compounds on the evolution of CBS lesions in orange fruits; to study the effects of VOC on resistance induction in orange fruits; to evaluate the effects of VOC on P. citricarpa morphology in CBS lesions, and to identify the produced VOC. Tryptone soya agar (TSA) and tryptone soya broth (TSB) media used to culture the bacterium resulted in up to 73% pathogen inhibition by VOC. Volatile compounds from Bacillus spp. ACB-65 and Bacillus spp. ACB-73 when cultured in TSB culture medium provided 86% inhibition of freckles that evolved to hard spots. The volatile fractions produced by the bacteria were identified as alcohols, ketones, amines, ethers, aldehydes and carboxylic acids that can serve as arsenal against the phytopathogen. The present work demonstrated the potential of VOC produced by Bacillus spp. in the control of P. citricarpa.},

keywords = {biological control, Citrus sinensis, Enzymatic activities, Guignardia citricarpa, Volatile organic compounds},

pubstate = {published},

tppubtype = {article}

}

@article{Shimizu2022,

title = {Chemical and microbiological changes in the soil mediated by different vegetative coverings in a Natal orange orchard},

author = {Gabriel Danilo Shimizu and Jean Carlo Baudraz Paula and Adriana Pereira Silva and Camilla Andrade Pacheco and Fernando Alves Azevedo and Leandro Simões Azeredo Gonçalves and Carmen Silvia Vieira Janeiro Neves},

url = {https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44132},

doi = {10.5433/1679-0359.2022V43N1P331},

issn = {1679-0359},

year  = {2022},

date = {2022-01-01},

journal = {Semina: Ciências Agrárias},

volume = {43},

issue = {1},

pages = {331-350},

publisher = {Universidade Estadual de Londrina},

abstract = {Proper soil cover management for citrus cultivation can contribute to increased productivity and improved soil quality. This study examined five different vegetative coverings [Urochloa brizantha; U. decumbens, U. ruziziensis, spontaneous vegetation, and herbicide application (glyphosate) in the total area] in the inter rows of a Natal orange orchard [Citrus sinensis (L.) Osbeck] grafted on the Swingle citrumelo (C. paradisi × Poncirus trifoliata). Their effects on the microbiological and chemical attributes of the soil and the vegetative development in the orchard were examined. Chemical (Ca2+, Mg2+, K+, P, pH, H+Al, CECpH7, base saturation, and OM) and microbiological (carbon and nitrogen of microbial biomass, basal respiration, and metabolic quotient) soil attributes in the rows and inter-rows were evaluated for the orchard in 2018 and 2019. There was a significant difference for most variables in the 2 years studied, emphasizing 2019 for microbiological parameters and OM, with the latter being 14.8% lower in the treatment with glyphosate in the total area compared to the treatment with spontaneous vegetation. The results showed the benefits of vegetation cover with brachiaria in inter-rows of the Natal sweet orange orchard in the chemical and microbiological attributes of the soil, especially in carbon and nitrogen of the microbial biomass.},

keywords = {Citrus sinensis, conservation agriculture, Glyphosate, Urochloa.},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Citrus Variegated Chlorosis: an Overview of 30 Years of Research and Disease Management},

author = {Helvecio Della Coletta-Filho and Andreina I. Castillo and Francisco Ferraz Laranjeira and Eduardo Chumbinho Andrade and Natalia Teixeira Silva and Alessandra Alves Souza and Mariana Esteves Bossi and Rodrigo P. P. Almeida and João R. S. Lopes},

url = {https://link.springer.com/article/10.1007/s40858-020-00358-5},

doi = {10.1007/S40858-020-00358-5/METRICS},

issn = {19832052},

year  = {2020},

date = {2020-01-01},

journal = {Tropical Plant Pathology},

volume = {45},

issue = {3},

pages = {175-191},

publisher = {Springer},

abstract = {The emergence of citrus variegated chlorosis (CVC) disease had dramatic consequences to the citrus industry in Brazil. First reported in São Paulo State in 1987, this disease affected approximately 100 million sweet orange trees in the region 20 years later. However, current estimates indicate that the number of diseased trees has been reduced 25-fold since 2009. In this review we summarize research on CVC since its emergence, focusing on work that has contributed to the observed success in managing this disease in the field. Knowledge that CVC is caused by a bacterium (Xylella fastidiosa - now classified as X. fastidiosa subsp. pauca) that is transmited by infected plant material (grafting and nursery plant) and insect vectors, the citrus nursery production system switched in 2003 to a certification program in which plants are grown in insect proof screen-houses and routinely monitored for X. fastidiosa infection. Research triggered by the genome sequencing of a CVC isolate in 2000, the first plant pathogenic bacterium to have its complete genome sequenced, integrated molecular tools and approaches into research aimed at understanding the biology of this pathogen. Ultimately, the challenges imposed by CVC led to significant improvements in the scientific and technical knowledge linked to sweet orange production, and to the development of a more sustainable and resilient citrus industry in Brazil.},

keywords = {Citrus bacterium disease, Citrus sinensis, Xylella fastidiosa},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Citrus Variegated Chlorosis: an Overview of 30 Years of Research and Disease Management},

author = {Helvecio Della Coletta-Filho and Andreina I. Castillo and Francisco Ferraz Laranjeira and Eduardo Chumbinho Andrade and Natalia Teixeira Silva and Alessandra Alves Souza and Mariana Esteves Bossi and Rodrigo P. P. Almeida and João R. S. Lopes},

url = {https://link.springer.com/article/10.1007/s40858-020-00358-5},

doi = {10.1007/S40858-020-00358-5/METRICS},

issn = {19832052},

year  = {2020},

date = {2020-01-01},

journal = {Tropical Plant Pathology},

volume = {45},

issue = {3},

pages = {175-191},

publisher = {Springer},

abstract = {The emergence of citrus variegated chlorosis (CVC) disease had dramatic consequences to the citrus industry in Brazil. First reported in São Paulo State in 1987, this disease affected approximately 100 million sweet orange trees in the region 20 years later. However, current estimates indicate that the number of diseased trees has been reduced 25-fold since 2009. In this review we summarize research on CVC since its emergence, focusing on work that has contributed to the observed success in managing this disease in the field. Knowledge that CVC is caused by a bacterium (Xylella fastidiosa - now classified as X. fastidiosa subsp. pauca) that is transmited by infected plant material (grafting and nursery plant) and insect vectors, the citrus nursery production system switched in 2003 to a certification program in which plants are grown in insect proof screen-houses and routinely monitored for X. fastidiosa infection. Research triggered by the genome sequencing of a CVC isolate in 2000, the first plant pathogenic bacterium to have its complete genome sequenced, integrated molecular tools and approaches into research aimed at understanding the biology of this pathogen. Ultimately, the challenges imposed by CVC led to significant improvements in the scientific and technical knowledge linked to sweet orange production, and to the development of a more sustainable and resilient citrus industry in Brazil.},

keywords = {Citrus bacterium disease, Citrus sinensis, Xylella fastidiosa},

pubstate = {published},

tppubtype = {article}

}

@article{Cunha2020,

title = {The action of yeast strains as biocontrol agents against Penicillium digitatum in Lima sweet oranges},

author = {Tatiane Cunha and Luriany Pompeo Ferraz and Gilmar Katia Cristina Kupper and Tatiane Cunha and Luriany Pompeo Ferraz and Gilmar Katia Cristina Kupper},

url = {http://citrusrt.ccsm.br/journal/citrusrt/article/doi/10.4322/crt.18819},

doi = {10.4322/CRT.18819},

issn = {21775419},

year  = {2020},

date = {2020-01-01},

journal = {Citrus Research & Technology},

volume = {41},

pages = {1-9},

publisher = {Centro de Citricultura Sylvio Moreira/Instituto Agronômico},

abstract = {The green mold caused by Penicillium digitatum is the most important disease affecting citrus in the postharvest period. An alternative to control the disease lies on the use of yeasts. The purpose of the present study was to assess the ability of 101 yeasts strains to control P. digitatumin vitro and in vivo. The assay followed a randomized design with five repetitions and the evaluation took into consideration the mean pathogen colony diameter in Petri dish paired culture. In addition, the effect of the fungus on yeast strain growth was assessed. Orange fruits (Citrus sinensis cv. Lima) were injured in two equidistant points and inoculated with P. digitatum conidia suspension 24 hours before and after treatments, in the assays conducted in vivo. Treatments consisted in the application of eight yeast suspensions, fungicide and distilled water (control treatment); they followed a randomized design with three repetitions (20 fruits per repetition). The best antagonist results in vitro were recorded for five strains (Saccharomyces cerevisiae ACBL-76 and ACBL-82 strains; Candida stellimalicola ACBL-84 strain; and ACBL-87), which presented mycelial growth inhibition values higher than 80%. Two yeast strains (S. cerevisiae ACBL-82 strain and Meyerozyma caribbica ACBL-86 strain) have successfully controlled the progress and incidence of green mold in orange fruits when they were applied in a preventive manner.},

keywords = {antagonist yeasts, biological control, Citrus sinensis, Index terms: green mold, postharvest},

pubstate = {published},

tppubtype = {article}

}

@article{Dorta2020,

title = {Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs},

author = {Sílvia Oliveira Dorta and Juliana Balbinotte and Rose Monnerat and João Roberto Spotti Lopes and Tatiane Cunha and Odimar Zanuzo Zanardi and Marcelo Pedreira Miranda and Marcos Antonio Machado and Juliana Freitas-Astúa},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/1744-7917.12654 https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-7917.12654 https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12654},

doi = {10.1111/1744-7917.12654},

issn = {1744-7917},

year  = {2020},

date = {2020-01-01},

journal = {Insect Science},

volume = {27},

issue = {3},

pages = {519-530},

publisher = {John Wiley & Sons, Ltd},

abstract = {Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of “Candidatus Liberibacter spp.,” associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%–77% and 66%–90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.},

keywords = {Asian citrus psyllid, Citrus sinensis, Cry toxins, endophytic Bt, huanglongbing, young shoots},

pubstate = {published},

tppubtype = {article}

}

@article{Kupper2020,

title = {Production of antifungal compounds by Bacillus spp. isolates and its capacity for controlling citrus black spot under field conditions},

author = {Katia Cristina Kupper and Rafael Kupper Moretto and Andreia Fujimoto},

url = {https://link.springer.com/article/10.1007/s11274-019-2772-0},

doi = {10.1007/S11274-019-2772-0/METRICS},

issn = {15730972},

year  = {2020},

date = {2020-01-01},

journal = {World Journal of Microbiology and Biotechnology},

volume = {36},

issue = {1},

pages = {1-10},

publisher = {Springer},

abstract = {Orange juice is a major agricultural product, and oranges are among the most widely sold fresh fruits in several countries. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, affects almost every species of citrus, causing a esthetic depreciation of the fruit and fruit drop, with consequent financial loss for its in natura commercialization. Fungicides are the major control measure for CBS, but have limited efficiency and high cost, and give rise to fungal strains resistant to these products. This work assayed the production of antifungal compounds by Bacillus spp. isolates and tested the potential of two isolates from B. subtilis (ACB-AP3 and ACB-83) for controlling CBS under field conditions with two previously untested orange varieties. A total of 15 isolates produced cell-free, thermostable or volatile compounds effective in suppressing P. citricarpa growth in vitro. It was possible to detect the production of two antibiotics (iturin and surfactin) by B. subtilis ACB-83. Additionally, B. subtilis isolates ACB-AP3 and ACB-83 displayed significant effects in controlling the disease under field conditions.},

keywords = {Cell-free antifungal compounds, Citrus sinensis, Liquid chromatography, mass spectrometry, Phyllosticta citricarpa, Thermostable, Volatile},

pubstate = {published},

tppubtype = {article}

}

@article{Fadel2019,

title = {Compatibility and horticultural performance of Pera sweet orange clones grafted to Swingle citrumelo rootstock},

author = {André Luiz Fadel and Eduardo Sanches Stuchi and Simone Rodrigues Silva and Luiz Gustavo Parolin and Carolina Rossi Oliveira and Gerd Walter Müller and Luiz Carlos Donadio},

url = {http://www.scielo.br/j/brag/a/k6vn876L4jPrNKR6ypvK9Ng/?lang=en},

doi = {10.1590/1678-4499.20190116},

issn = {0006-8705},

year  = {2019},

date = {2019-01-01},

journal = {Bragantia},

volume = {78},

issue = {4},

pages = {564-572},

publisher = {Instituto Agronômico de Campinas},

abstract = {ABSTRACT Despite its interesting agricultural traits, Swingle citrumelo (Citrus paradisi Macfad. ‘Duncan’ × Poncirus trifoliata L. Raf.) rootstock exhibits severe incompatibility when grafted with Pera sweet orange [Citrus sinensis (L.) Osb.] scions, and this fact limits its use for propagation purposes. Nonetheless, the existence of certain Pera sweet orange clones that are compatible with Swingle citrumelo represents a substantial gain for the entire citrus industry. This study investigated the compatibility between seven Pera sweet orange clones and Swingle citrumelo rootstock, regarding the presence of a necrotic line at the graft union junction, the difference in diameter between rootstock and scion, the influence of clonal cleaning by micrografting, and the horticultural performance of the selected combinations. No trees died because of grafting incompatibility, even after twenty years (trial I), except for clone CV1, or after six years and nine months (trial II) from planting. The rootstock/scion trunk diameter ratio was not related to the presence of a necrotic line in the graft-union region. Additionally, these variables did not influence fruit yield and quality, and they were not affected by clonal cleaning or pre-immunisation of the old Pera sweet orange clones.},

keywords = {bud, Citrus paradisi × Poncirus trifoliata, Citrus sinensis, rootstock, union crease},

pubstate = {published},

tppubtype = {article}

}

@article{Kirinus2019,

title = {Agronomic performance of 'Valência' orange combined with 13 rootstocks in the state of Rio Grande do Sul, Brazil},

author = {Marines Batalha Moreno Kirinus and Roberto Pedroso Oliveira and Pricila Santos Silva and Caroline Farias Barreto and Marcelo Barbosa Malgarim and Walter Santos Soares Filho},

url = {http://www.scielo.br/j/pab/a/4WkkddvbqKLSj6yTF8S9hDF/?lang=en},

doi = {10.1590/S1678-3921.PAB2019.V54.00461},

issn = {0100-204X},

year  = {2019},

date = {2019-01-01},

journal = {Pesquisa Agropecuária Brasileira},

volume = {54},

publisher = {Embrapa Secretaria de Pesquisa e Desenvolvimento, Pesquisa Agropecuária Brasileira},

abstract = {Abstract: The objective of this work was to evaluate the agronomic performance of 'Valência' sweet orange (Citrus sinensis) tree, in combination with 13 rootstocks cultivated in soil and climate conditions of the Brazilian state of Rio Grande do Sul. The following agronomic variables were evaluated: scion height and volume, plant production, production efficiency, productivity, fruit diameter, fruit mass, peel mass, juice yield, soluble solids content, titratable acidity, soluble solids:acidity ratio, ascorbic acid, peel color, antioxidant capacity, and phenolic compounds. A randomized complete block experimental design was used, with three replicates and nine plants per plot, in a two-factor arrangement (13 rootstocks and the 2015 and 2016 harvests). The following rootstocks stood out: TSKC × TRDP-023 for its juice yield (54.0 and 51.7%), soluble solids:acidity ratio (8.66 and 6.99), and ascorbic acid (49.3 and 51 mg 100 g-1); and the citrandarin 'San Diego', for its higher phenolic-compound content (106.4 and 74.9 100 g-1 gallic acid), coloration (76.2 and 75.9 hº), and ascorbic acid (42.2 and 44.7 mg 100 g-1). The TSKC × TRDP-026 and TSKC × CTQT1434-004 rootstocks induce canopy formation and productive precocity in 'Valência' orange trees grown in the southern region of the state of Rio Grande do Sul state, in the first few years of growth.},

keywords = {bioactive compounds, Citrus sinensis, Fruit quality},

pubstate = {published},

tppubtype = {article}

}

@article{Shimo2019,

title = {Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance},

author = {Hugo Massayoshi Shimo and Carolina Terassi and Caio Cesar Lima Silva and Jackeline Lima Zanella and Gustavo Fernando Mercaldi and Silvana Aparecida Rocco and Celso Eduardo Benedetti},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12815 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12815 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12815},

doi = {10.1111/MPP.12815},

issn = {1364-3703},

year  = {2019},

date = {2019-01-01},

journal = {Molecular Plant Pathology},

volume = {20},

issue = {8},

pages = {1105-1118},

publisher = {John Wiley & Sons, Ltd},

abstract = {Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unknown. Here, we show that the Citrus sinensis CAF1 (CsCAF1) is a magnesium-dependent deadenylase implicated in resistance against the citrus canker bacteria Xanthomonas citri. CsCAF1 interacted with proteins of the CCR4-NOT complex, including CsVIP2, a NOT2 homologue, translin-associated factor X (CsTRAX) and the poly(A)-binding proteins CsPABPN and CsPABPC. CsCAF1 also interacted with PthA4, the main X. citri effector required for citrus canker elicitation. We also present evidence suggesting that PthA4 inhibits CsCAF1 deadenylase activity in vitro and stabilizes the mRNA encoded by the citrus canker susceptibility gene CsLOB1, which is transcriptionally activated by PthA4 during canker formation. Moreover, we show that an inhibitor of CsCAF1 deadenylase activity significantly enhanced canker development, despite causing a reduction in PthA4-dependent CsLOB1 transcription. These results thus link CsCAF1 with canker development and PthA4-dependent transcription in citrus plants.},

keywords = {associated factor, CCR4, Citrus canker, Citrus sinensis, CsCAF1, CsLOB1, NOT, PthA4, RNA deadenylase activity, TAL effectors, Xanthomonas aurantifolii, Xanthomonas citri},

pubstate = {published},

tppubtype = {article}

}

@article{Teodoro2019,

title = {Performance of 'Jaffa' sweet orange on different rootstocks for orchards in the Brazilian Northeast},

author = {Adenir Vieira Teodoro and Hélio Wilson Lemos Carvalho and Inácio Barros and Luciana Marques Carvalho and Carlos Roberto Martins and Walter Santos Soares Filho and Eduardo Augusto Girardi and Orlando Sampaio Passos},

url = {http://www.scielo.br/j/pab/a/RDvfssQbk63xpVxpnggLWBr/?lang=en},

doi = {10.1590/S1678-3921.PAB2020.V55.01665},

issn = {0100-204X},

year  = {2019},

date = {2019-01-01},

journal = {Pesquisa Agropecuária Brasileira},

volume = {55},

pages = {2020},

publisher = {Embrapa Secretaria de Pesquisa e Desenvolvimento, Pesquisa Agropecuária Brasileira},

abstract = {Abstract: The objective of this work was to evaluate the effect of nine rootstocks on 'Jaffa' sweet orange (Citrus sinensis) for its vegetative, productive, and qualitative traits, and for its susceptibility to three pest mites (Eutetranychus banksi, Tetranychus mexicanus and Phyllocoptruta oleivora). The following parameters were evaluated: vegetative growth, yield, physiochemical characteristics of fruit, and density of pest mites. 'Sunki Tropical' mandarin conferred high cumulative yields whereas 'Orlando' tangelo and 'Rugoso Vermelho' lemon imparted bigger fruit with low brix, and less acidity to 'Jaffa'. Indio and 'Riverside' citrandarins induced more acid fruit of smaller sizes, as well as low-cumulated yields and canopy volumes. Also, the 'Cravo Santa Cruz' lime, 'San Diego' citrandarin, and the hybrids HTR-051 and LVK x LCR-010 conferred lower-cumulated yields to 'Jaffa'. The rootstocks did not influence the population levels of the evaluated mites. 'Sunki Tropical' mandarin, 'Cravo Santa Cruz' lime and 'Rugoso Vermelho' lemon stand up as excellent rootstock options for 'Jaffa' sweet orange in the Northeastern Brazil.},

keywords = {Citrus sinensis, juice content, phytophagous mites, Yield},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum},

author = {Tatiane Cunha and Luriany Pompeo Ferraz and Pitt Paul Wehr and Katia Cristina Kupper},

doi = {10.1016/J.IJFOODMICRO.2018.03.019},

issn = {0168-1605},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Food Microbiology},

volume = {276},

pages = {20-27},

publisher = {Elsevier},

abstract = {Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.},

keywords = {‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum},

author = {Tatiane Cunha and Luriany Pompeo Ferraz and Pitt Paul Wehr and Katia Cristina Kupper},

doi = {10.1016/J.IJFOODMICRO.2018.03.019},

issn = {0168-1605},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Food Microbiology},

volume = {276},

pages = {20-27},

publisher = {Elsevier},

abstract = {Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.},

keywords = {‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists},

pubstate = {published},

tppubtype = {article}

}

@article{Andrade2018,

title = {Space–time variability of citrus leprosis as strategic planning for crop management},

author = {Daniel J. Andrade and José R. Lorençon and Diego S. Siqueira and Valdenice M. Novelli and Renato B. Bassanezi},

url = {https://onlinelibrary.wiley.com/doi/full/10.1002/ps.4877 https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4877 https://onlinelibrary.wiley.com/doi/10.1002/ps.4877},

doi = {10.1002/PS.4877},

issn = {1526-4998},

year  = {2018},

date = {2018-01-01},

journal = {Pest Management Science},

volume = {74},

issue = {8},

pages = {1798-1803},

publisher = {John Wiley & Sons, Ltd},

abstract = {BACKGROUND: Citrus leprosis is the most important viral disease of citrus. Knowledge of its spatiotemporal structure is fundamental to a representative sampling plan focused on the disease control approach. Such a well-crafted sampling design helps to reduce pesticide use in agriculture to control pests and diseases. RESULTS: Despite the use of acaricides to control citrus leprosis vector (Brevipalpus spp.) populations, the disease has spread rapidly through experimental areas. Citrus leprosis has an aggregate spatial distribution, with high dependence among symptomatic plants. Temporal variation in disease incidence increased among symptomatic plants by 4% per month. CONCLUSIONS: Use of acaricides alone to control the vector of leprosis is insufficient to avoid its incidence in healthy plants. Preliminary investigation into the time and space variation in the incidence of the disease is fundamental to select a sampling plan and determine effective strategies for disease management. © 2018 Society of Chemical Industry.},

keywords = {Brevipalpus spp, CiLV, Citrus leprosis virus, Citrus sinensis, epidemiology, geostatistics},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum},

author = {Tatiane Cunha and Luriany Pompeo Ferraz and Pitt Paul Wehr and Katia Cristina Kupper},

doi = {10.1016/J.IJFOODMICRO.2018.03.019},

issn = {0168-1605},

year  = {2018},

date = {2018-01-01},

journal = {International Journal of Food Microbiology},

volume = {276},

pages = {20-27},

publisher = {Elsevier},

abstract = {Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.},

keywords = {‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists},

pubstate = {published},

tppubtype = {article}

}