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2021
Santos, Ariana Silva; Andrade, Edson Mário; Sousa, Andressa Rodrigues Oliveira; Costa, Marcos Mota Carmo; Togawa, Roberto; Grynberg, Priscila; Pirovani, Carlos Priminho; Oliveira, Saulo Alves Silva; Filho, Walter Santos Soares; Filho, Mauricio Antônio Coelho; Gesteira, Abelmon Silva; Ferreira, Claudia Fortes
Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies Journal Article
Em: Scientia Horticulturae, vol. 277, pp. 109838, 2021, ISSN: 0304-4238.
Resumo | Links | BibTeX | Tags: Citrus, drought, RNA-Seq, root, Sunki, Transcriptome
@article{nokey,
title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},
author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},
doi = {10.1016/J.SCIENTA.2020.109838},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {277},
pages = {109838},
publisher = {Elsevier},
abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},
keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},
pubstate = {published},
tppubtype = {article}
}
Santos, Ariana Silva; Andrade, Edson Mário; Sousa, Andressa Rodrigues Oliveira; Costa, Marcos Mota Carmo; Togawa, Roberto; Grynberg, Priscila; Pirovani, Carlos Priminho; Oliveira, Saulo Alves Silva; Filho, Walter Santos Soares; Filho, Mauricio Antônio Coelho; Gesteira, Abelmon Silva; Ferreira, Claudia Fortes
Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies Journal Article
Em: Scientia Horticulturae, vol. 277, pp. 109838, 2021, ISSN: 0304-4238.
Resumo | Links | BibTeX | Tags: Citrus, drought, RNA-Seq, root, Sunki, Transcriptome
@article{nokey,
title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},
author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},
doi = {10.1016/J.SCIENTA.2020.109838},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {277},
pages = {109838},
publisher = {Elsevier},
abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},
keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},
pubstate = {published},
tppubtype = {article}
}
2020
Meneses, Thaís N.; Filho, Mauricio A. Coelho; Filho, Hermes P. Santos; de A. Dos Santos, Luana L.; Gesteira, Abelmon S.; Filho, Walter Dos S. Soares
Rootstocks and planting types on root architecture and vegetative vigor of ‘Pera’ sweet orange trees Journal Article
Em: Revista Brasileira de Engenharia Agrícola e Ambiental, vol. 24, iss. 10, pp. 685-693, 2020, ISSN: 1415-4366.
Resumo | Links | BibTeX | Tags: citriculture, cohesive soil, Drought tolerance, root
@article{Meneses2020,
title = {Rootstocks and planting types on root architecture and vegetative vigor of ‘Pera’ sweet orange trees},
author = {Thaís N. Meneses and Mauricio A. Coelho Filho and Hermes P. Santos Filho and Luana L. de A. Dos Santos and Abelmon S. Gesteira and Walter Dos S. Soares Filho},
url = {http://www.scielo.br/j/rbeaa/a/dFHKYJKmBXFkz7SSRBzcp5x/?lang=en},
doi = {10.1590/1807-1929/AGRIAMBI.V24N10P685-693},
issn = {1415-4366},
year = {2020},
date = {2020-01-01},
journal = {Revista Brasileira de Engenharia Agrícola e Ambiental},
volume = {24},
issue = {10},
pages = {685-693},
publisher = {Departamento de Engenharia Agrícola - UFCG},
abstract = {ABSTRACT Root architecture affects plant growth and agricultural productions. Although it is related to genetic factors, soil physical limitations can affect the root system and volume of explored soil, compromising plant growth and fruit production. Considering the need to mitigate these restrictions, the objective of the present study was to evaluate two planting types (planting of protected seedlings and planting of seeds in definitive place) and two scion-rootstock combinations (‘Pera’ sweet orange, CNPMF D-6 selection, combined with ‘Santa Cruz Rangpur’ lime and ‘Sunki Tropical’ mandarin rootstocks). The experiment was conducted at the Lagoa do Coco Farm, Rio Real, BA, Brazil, in a Oxisol under rainfed conditions. Biometric, physiological, and soil water content evaluations were made during the experiment. Soil mechanical resistance to root penetration and root system characteristics were also evaluated. Roots were concentrated on the soil surface layers (0-0.40 m), extending horizontally up to 1.50 m from the plant, regardless of the management practices used. The total root length in each root diameter classes studied, root length density, and total root mean diameter were higher for planting of protected seedling, resulting in greater root development. Plants from ‘Pera’ sweet orange scion grafted on ‘Santa Cruz Rangpur’ lime rootstock had earlier production and were more vigorous. Soil structure affects root growth, and the root distribution up to 0.40 m depth is correlated with plant vigor, yield, and water status under rainfed conditions.},
keywords = {citriculture, cohesive soil, Drought tolerance, root},
pubstate = {published},
tppubtype = {article}
}