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2019
Ferrarezi, Juliano Henrique; Santos, Juliana Aparecida; Sette, Lara Durães; Ferreira, Henrique; Sass, Daiane Cristina
Anti-Xanthomonas activity of Antarctic fungi crude extracts Journal Article
Em: African Journal of Biotechnology, vol. 18, iss. 28, pp. 713-718, 2019, ISSN: 1684-5315.
Resumo | Links | BibTeX | Tags: Antarctic fungi, antibacterial action, Citrus canker, leaf scald, natural extracts
@article{nokey,
title = {Anti-Xanthomonas activity of Antarctic fungi crude extracts},
author = {Juliano Henrique Ferrarezi and Juliana Aparecida Santos and Lara Durães Sette and Henrique Ferreira and Daiane Cristina Sass},
url = {https://academicjournals.org/journal/AJB/article-abstract/A2117D861600},
doi = {10.5897/AJB2019.16886},
issn = {1684-5315},
year = {2019},
date = {2019-01-01},
journal = {African Journal of Biotechnology},
volume = {18},
issue = {28},
pages = {713-718},
publisher = {Academic Journals},
abstract = {Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves there are no satisfactory control. Due to the phytosanitary problem caused by Xanthomonas (X. citri and X. albilineans) and by the difficulty of its control, the search for new forms of defense, less harmful to the environment, has become increasingly required. Thus, the aim of this work was to obtain crude organic extracts from filamentous fungi isolated from Antarctic soil samples and assess its bioactivity potential against X. citri and X. albilineans. One hundred and twenty-two extracts were tested, seven extracts inhibited the cell growth of X. citri, one was bioactive only against X. albilineans and one extract inhibited the cell growth of both bacteria. The bioactive extracts had a mean inhibition value of 96% against both bacteria. The values of MIC90 and MBC of bioactive extracts were also determined; for X. citri the isolate 3.1 Fe presented the lowest values of MIC90 (0.28 mg/mL) and MBC (1.0 mg/mL) and the two bioactive isolates for X. albilineans (1.1-Fe and B-Fe) presented the same values of MIC90 (1.4 mg/mL) and MBC (1.5 mg/mL). The filamentous fungi that produced positive extracts were identified as belonging to the genus Pseudogymnoascus (n=8) and Cladosporium (n=1). The filamentous fungi isolated from Antarctic soil produced compounds with bioactivity against phytopathogens from the Xanthomonas genus.
Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.
},
keywords = {Antarctic fungi, antibacterial action, Citrus canker, leaf scald, natural extracts},
pubstate = {published},
tppubtype = {article}
}
Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves there are no satisfactory control. Due to the phytosanitary problem caused by Xanthomonas (X. citri and X. albilineans) and by the difficulty of its control, the search for new forms of defense, less harmful to the environment, has become increasingly required. Thus, the aim of this work was to obtain crude organic extracts from filamentous fungi isolated from Antarctic soil samples and assess its bioactivity potential against X. citri and X. albilineans. One hundred and twenty-two extracts were tested, seven extracts inhibited the cell growth of X. citri, one was bioactive only against X. albilineans and one extract inhibited the cell growth of both bacteria. The bioactive extracts had a mean inhibition value of 96% against both bacteria. The values of MIC90 and MBC of bioactive extracts were also determined; for X. citri the isolate 3.1 Fe presented the lowest values of MIC90 (0.28 mg/mL) and MBC (1.0 mg/mL) and the two bioactive isolates for X. albilineans (1.1-Fe and B-Fe) presented the same values of MIC90 (1.4 mg/mL) and MBC (1.5 mg/mL). The filamentous fungi that produced positive extracts were identified as belonging to the genus Pseudogymnoascus (n=8) and Cladosporium (n=1). The filamentous fungi isolated from Antarctic soil produced compounds with bioactivity against phytopathogens from the Xanthomonas genus.
Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.
Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.
Ferrarezi, Juliano Henrique; Santos, Juliana Aparecida; Sette, Lara Durães; Ferreira, Henrique; Sass, Daiane Cristina
Anti-Xanthomonas activity of Antarctic fungi crude extracts Journal Article
Em: African Journal of Biotechnology, vol. 18, iss. 28, pp. 713-718, 2019, ISSN: 1684-5315.
Resumo | Links | BibTeX | Tags: Antarctic fungi, antibacterial action, Citrus canker, leaf scald, natural extracts
@article{nokey,
title = {Anti-Xanthomonas activity of Antarctic fungi crude extracts},
author = {Juliano Henrique Ferrarezi and Juliana Aparecida Santos and Lara Durães Sette and Henrique Ferreira and Daiane Cristina Sass},
url = {https://academicjournals.org/journal/AJB/article-abstract/A2117D861600},
doi = {10.5897/AJB2019.16886},
issn = {1684-5315},
year = {2019},
date = {2019-01-01},
journal = {African Journal of Biotechnology},
volume = {18},
issue = {28},
pages = {713-718},
publisher = {Academic Journals},
abstract = {Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves there are no satisfactory control. Due to the phytosanitary problem caused by Xanthomonas (X. citri and X. albilineans) and by the difficulty of its control, the search for new forms of defense, less harmful to the environment, has become increasingly required. Thus, the aim of this work was to obtain crude organic extracts from filamentous fungi isolated from Antarctic soil samples and assess its bioactivity potential against X. citri and X. albilineans. One hundred and twenty-two extracts were tested, seven extracts inhibited the cell growth of X. citri, one was bioactive only against X. albilineans and one extract inhibited the cell growth of both bacteria. The bioactive extracts had a mean inhibition value of 96% against both bacteria. The values of MIC90 and MBC of bioactive extracts were also determined; for X. citri the isolate 3.1 Fe presented the lowest values of MIC90 (0.28 mg/mL) and MBC (1.0 mg/mL) and the two bioactive isolates for X. albilineans (1.1-Fe and B-Fe) presented the same values of MIC90 (1.4 mg/mL) and MBC (1.5 mg/mL). The filamentous fungi that produced positive extracts were identified as belonging to the genus Pseudogymnoascus (n=8) and Cladosporium (n=1). The filamentous fungi isolated from Antarctic soil produced compounds with bioactivity against phytopathogens from the Xanthomonas genus.
Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.
},
keywords = {Antarctic fungi, antibacterial action, Citrus canker, leaf scald, natural extracts},
pubstate = {published},
tppubtype = {article}
}
Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves there are no satisfactory control. Due to the phytosanitary problem caused by Xanthomonas (X. citri and X. albilineans) and by the difficulty of its control, the search for new forms of defense, less harmful to the environment, has become increasingly required. Thus, the aim of this work was to obtain crude organic extracts from filamentous fungi isolated from Antarctic soil samples and assess its bioactivity potential against X. citri and X. albilineans. One hundred and twenty-two extracts were tested, seven extracts inhibited the cell growth of X. citri, one was bioactive only against X. albilineans and one extract inhibited the cell growth of both bacteria. The bioactive extracts had a mean inhibition value of 96% against both bacteria. The values of MIC90 and MBC of bioactive extracts were also determined; for X. citri the isolate 3.1 Fe presented the lowest values of MIC90 (0.28 mg/mL) and MBC (1.0 mg/mL) and the two bioactive isolates for X. albilineans (1.1-Fe and B-Fe) presented the same values of MIC90 (1.4 mg/mL) and MBC (1.5 mg/mL). The filamentous fungi that produced positive extracts were identified as belonging to the genus Pseudogymnoascus (n=8) and Cladosporium (n=1). The filamentous fungi isolated from Antarctic soil produced compounds with bioactivity against phytopathogens from the Xanthomonas genus.
Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.
Key words: Antarctic fungi; antibacterial action; citrus canker; leaf scald; natural extracts.
2018
Vieira, G.; Purić, J.; Morão, L. G.; Santos, J. A.; Inforsato, F. J.; Sette, L. D.; Ferreira, H.; Sass, D. C.
Terrestrial and marine Antarctic fungi extracts active against Xanthomonas citri subsp. citri Journal Article
Em: Letters in Applied Microbiology, vol. 67, iss. 1, pp. 64-71, 2018, ISSN: 1472-765X.
Resumo | Links | BibTeX | Tags: Antarctic fungi, antibacterial action, Citrus canker, Secondary metabolites, Xanthomonas
@article{Vieira2018,
title = {Terrestrial and marine Antarctic fungi extracts active against Xanthomonas citri subsp. citri},
author = {G. Vieira and J. Purić and L. G. Morão and J. A. Santos and F. J. Inforsato and L. D. Sette and H. Ferreira and D. C. Sass},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/lam.12890 https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.12890 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/lam.12890},
doi = {10.1111/LAM.12890},
issn = {1472-765X},
year = {2018},
date = {2018-01-01},
journal = {Letters in Applied Microbiology},
volume = {67},
issue = {1},
pages = {64-71},
publisher = {John Wiley & Sons, Ltd},
abstract = {This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctica and assess its potential antibacterial activity on Xanthomonas citri subsp. citri, the agent of citrus canker. Metabolites production was conducted in Malt 2% broth at 15°C for 20 days after which intracellular and extracellular extracts were obtained. The extracts were evaluated by cell viability assays through Resazurin Microtitre Assay. From 158 fungal extracts, 33 hampered bacterial growth in vitro. The average inhibition of the extracts obtained from terrestrial (soil) and marine (sediments) fungi was 94 and 97% respectively. These inhibition values were close to the average of 90% cell death for the positive control. MIC90 and MBC for the bioactive extracts were established. Isolates that produced active metabolites against the phytopathogen were identified using molecular taxonomy (ITS-rRNA sequencing) as: Pseudogymnoascus, Penicillium, Cadophora, Paraconiothyrium and Toxicocladosporium. Antarctic fungal strains isolated from terrestrial and marine sediments were able to produce secondary metabolites with antimicrobial activity against X. citri subsp. citri, highlighting the importance of these microbial genetic resources. These metabolites have potential to be used as alternatives for the control of this plant pathogen. Significance and Impact of the Study: This manuscript makes an impact on the study of micro-organisms from extreme habitats and their possible contribution in discovering new active molecules against pathogens of agricultural interest. Studies on the Antarctic continent and its communities have attracted the scientific community due to the long period of isolation and low levels of disturbance that surrounds the region. Knowing the potential of fungi in this region to produce active secondary metabolites, we aim to contribute to the discovery of compounds with antibacterial action in Xanthomonas citri subsp. citri, a plant pathogen present in several regions around the globe.},
keywords = {Antarctic fungi, antibacterial action, Citrus canker, Secondary metabolites, Xanthomonas},
pubstate = {published},
tppubtype = {article}
}
This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctica and assess its potential antibacterial activity on Xanthomonas citri subsp. citri, the agent of citrus canker. Metabolites production was conducted in Malt 2% broth at 15°C for 20 days after which intracellular and extracellular extracts were obtained. The extracts were evaluated by cell viability assays through Resazurin Microtitre Assay. From 158 fungal extracts, 33 hampered bacterial growth in vitro. The average inhibition of the extracts obtained from terrestrial (soil) and marine (sediments) fungi was 94 and 97% respectively. These inhibition values were close to the average of 90% cell death for the positive control. MIC90 and MBC for the bioactive extracts were established. Isolates that produced active metabolites against the phytopathogen were identified using molecular taxonomy (ITS-rRNA sequencing) as: Pseudogymnoascus, Penicillium, Cadophora, Paraconiothyrium and Toxicocladosporium. Antarctic fungal strains isolated from terrestrial and marine sediments were able to produce secondary metabolites with antimicrobial activity against X. citri subsp. citri, highlighting the importance of these microbial genetic resources. These metabolites have potential to be used as alternatives for the control of this plant pathogen. Significance and Impact of the Study: This manuscript makes an impact on the study of micro-organisms from extreme habitats and their possible contribution in discovering new active molecules against pathogens of agricultural interest. Studies on the Antarctic continent and its communities have attracted the scientific community due to the long period of isolation and low levels of disturbance that surrounds the region. Knowing the potential of fungi in this region to produce active secondary metabolites, we aim to contribute to the discovery of compounds with antibacterial action in Xanthomonas citri subsp. citri, a plant pathogen present in several regions around the globe.