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2020
Galdeano, Diogo Manzano; Pacheco, Inaiara Souza; Alves, Gustavo Rodrigues; Granato, Laís Moreira; Rashidi, Mahnaz; Turner, Donielle; Levy, Amit; Machado, Marcos Antonio
Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 6, pp. 559-571, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: ‘Candidatus Liberibacter asiaticus’, Asian citrus psyllid, Bacteria-vector interactions, huanglongbing, Multitrophic interaction, Vector-borne pathogens
@article{Galdeano2020,
title = {Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri},
author = {Diogo Manzano Galdeano and Inaiara Souza Pacheco and Gustavo Rodrigues Alves and Laís Moreira Granato and Mahnaz Rashidi and Donielle Turner and Amit Levy and Marcos Antonio Machado},
url = {https://link.springer.com/article/10.1007/s40858-020-00375-4},
doi = {10.1007/S40858-020-00375-4/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {559-571},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Interactions between insects and plant pathogens have been more enthusiastically studied in the recent decade, especially those relationships which takes the insects as vectors. The spectrum of these interactions ranges from mutualistic to pathogenic. The length of the co-evolutionary process will determine whether a microorganism shares a friend or a foe relationship with its host, and a friendship connection is frequently observed if the coexistence is longer. This review updates knowledge about the morphological, physiological and genetic mechanisms that drive the interaction between ‘Candidatus Liberibacter asiaticus’ (Las) and its vector, the Asian citrus psyllid, Diaphorina citri. Las is the predominant causal agent of citrus huanglongbing (HLB) disease, the major constrain to citrus production worldwide. This bacterium is transmitted by D. citri, in a propagative-circulative manner during its feeding from plant host. Understanding of the interactions among vector, plant pathogen and host plant are important for the management of this vector-borne disease complex.},
keywords = {‘Candidatus Liberibacter asiaticus’, Asian citrus psyllid, Bacteria-vector interactions, huanglongbing, Multitrophic interaction, Vector-borne pathogens},
pubstate = {published},
tppubtype = {article}
}
Interactions between insects and plant pathogens have been more enthusiastically studied in the recent decade, especially those relationships which takes the insects as vectors. The spectrum of these interactions ranges from mutualistic to pathogenic. The length of the co-evolutionary process will determine whether a microorganism shares a friend or a foe relationship with its host, and a friendship connection is frequently observed if the coexistence is longer. This review updates knowledge about the morphological, physiological and genetic mechanisms that drive the interaction between ‘Candidatus Liberibacter asiaticus’ (Las) and its vector, the Asian citrus psyllid, Diaphorina citri. Las is the predominant causal agent of citrus huanglongbing (HLB) disease, the major constrain to citrus production worldwide. This bacterium is transmitted by D. citri, in a propagative-circulative manner during its feeding from plant host. Understanding of the interactions among vector, plant pathogen and host plant are important for the management of this vector-borne disease complex.
Granato, Laís Moreira; Oliveira, Tiago S.; Boscariol-Camargo, Raquel L.; Galdeano, Diogo M.; Silva, Nicholas V.; Máximo, Heros J.; Dalio, Ronaldo J. D.; Machado, Marcos A.
Em: Tropical Plant Pathology, vol. 45, iss. 6, pp. 646-657, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: ‘Candidatus Liberibacter asiaticus’, Citrus HLB, Gene expression, Secretion, Virulence factors
@article{Granato2020,
title = {‘Candidatus Liberibacter asiaticus’ putative effectors: in silico analysis and gene expression in citrus leaves displaying distinct huanglongbing symptoms},
author = {Laís Moreira Granato and Tiago S. Oliveira and Raquel L. Boscariol-Camargo and Diogo M. Galdeano and Nicholas V. Silva and Heros J. Máximo and Ronaldo J. D. Dalio and Marcos A. Machado},
url = {https://link.springer.com/article/10.1007/s40858-020-00382-5},
doi = {10.1007/S40858-020-00382-5/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {646-657},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Huanglongbing (HLB) is considered the most devastating bacterial disease in citriculture. It is caused by the phloem-limited α-proteobacterium ‘Candidatus Liberibacter asiaticus’ (CLas). All citrus varieties are susceptible to HLB and many studies have been conducted in an attempt to identify potential targets involved in bacterial virulence and pathogenicity mechanisms. One of the most important virulence factors of bacterial pathogens is the effector molecule that can modify host cell structures, facilitate infection, and/or trigger host defense responses. In this study, 14 CLas candidates for secreted hypothetical proteins were predicted by a bioinformatics pipeline, one of which (NSEC01) was predicted to carry a nuclear localization sequence (NLS). Putative effectors HLBSP04 and HLBSP21 were conserved in all genomes evaluated, whereas HLBSP01, HLBSP19, and HLBSP30 were exclusively found in CLas. Expression analysis of candidate effector genes was performed in citrus leaves exhibiting five distinct HLB symptoms (asymptomatic, blotchy mottle, zinc deficiency, yellowed veins, and vein corky) and used as a guide for the selection of effectors for further functional studies. Genes coding for candidate effectors HLBSP13, HLBSP20, HLBSP28, and NSEC01 had high expression levels in blotchy mottle leaves, suggesting they could be used as marker genes that may facilitate detection of the pathogen in trees with this HLB symptom. As NSEC01 was exclusively found in this work, it was selected for transient expression in Nicotiana benthamiana leaves, but further investigations need to be carried out. Also, other candidate effectors predicted in this work still need to be functionally characterized. This study allowed discussing and proposing hypotheses about CLas classification according to virulence and lifestyle, which can help understand the citrus-CLas interaction.},
keywords = {‘Candidatus Liberibacter asiaticus’, Citrus HLB, Gene expression, Secretion, Virulence factors},
pubstate = {published},
tppubtype = {article}
}
Huanglongbing (HLB) is considered the most devastating bacterial disease in citriculture. It is caused by the phloem-limited α-proteobacterium ‘Candidatus Liberibacter asiaticus’ (CLas). All citrus varieties are susceptible to HLB and many studies have been conducted in an attempt to identify potential targets involved in bacterial virulence and pathogenicity mechanisms. One of the most important virulence factors of bacterial pathogens is the effector molecule that can modify host cell structures, facilitate infection, and/or trigger host defense responses. In this study, 14 CLas candidates for secreted hypothetical proteins were predicted by a bioinformatics pipeline, one of which (NSEC01) was predicted to carry a nuclear localization sequence (NLS). Putative effectors HLBSP04 and HLBSP21 were conserved in all genomes evaluated, whereas HLBSP01, HLBSP19, and HLBSP30 were exclusively found in CLas. Expression analysis of candidate effector genes was performed in citrus leaves exhibiting five distinct HLB symptoms (asymptomatic, blotchy mottle, zinc deficiency, yellowed veins, and vein corky) and used as a guide for the selection of effectors for further functional studies. Genes coding for candidate effectors HLBSP13, HLBSP20, HLBSP28, and NSEC01 had high expression levels in blotchy mottle leaves, suggesting they could be used as marker genes that may facilitate detection of the pathogen in trees with this HLB symptom. As NSEC01 was exclusively found in this work, it was selected for transient expression in Nicotiana benthamiana leaves, but further investigations need to be carried out. Also, other candidate effectors predicted in this work still need to be functionally characterized. This study allowed discussing and proposing hypotheses about CLas classification according to virulence and lifestyle, which can help understand the citrus-CLas interaction.