Publicações

@article{Esteves2020,

title = {XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca},

author = {Mariana Bossi Esteves and Julia Lopes Nalin and Karla Kudlawiec and Raquel Caserta Salviatto and Tiago Melo Sales and Anne Sicard and Rodrigo Piacentini Paes Almeida and Alessandra Alves Souza and João Roberto Spotti Lopes},

url = {https://www.mdpi.com/2075-4450/11/8/473/htm https://www.mdpi.com/2075-4450/11/8/473},

doi = {10.3390/INSECTS11080473},

issn = {2075-4450},

year  = {2020},

date = {2020-01-01},

journal = {Insects 2020, Vol. 11, Page 473},

volume = {11},

issue = {8},

pages = {473},

publisher = {Multidisciplinary Digital Publishing Institute},

abstract = {Xylella fastidiosa is a vector-borne bacterium that causes diseases in many plants of economic interest. The bacterium-vector initial interactions involve bacterial membrane-bound adhesins that mediate cell attachment to the foregut of insect vectors. We investigated the role of the afimbrial adhesin XadA2 in the binding and biofilm formation of X. fastidiosa subsp. pauca to vector surfaces in vitro, as well as its potential to disrupt pathogen transmission. We showed that XadA2 has binding affinity for polysaccharides on sharpshooter hindwings, used as a proxy for the interactions between X. fastidiosa and vectors. When in a medium without carbon sources, the bacterium used wing components, likely chitin, as a source of nutrients and formed a biofilm on the wing surface. There was a significant reduction in X. fastidiosa biofilm formation and cell aggregation on vector wings in competition assays with XadA2 or its specific antibody (anti-XadA2). Finally, pathogen acquisition and transmission to plant were significantly reduced when the vectors acquired X. fastidiosa from an artificial diet supplemented with anti-XadA2. These results show that XadA2 is important in mediating bacterial colonization in the insect and that it could be used as a target for blocking X. fastidiosa transmission.},

keywords = {attachment, bacterium, blocking transmission, borne, sharpshooter, vector},

pubstate = {published},

tppubtype = {article}

}

@article{Morelli2020,

title = {Impact of HLB on the physiological quality of citrus rootstock seeds and non-vertical ‘Candidatus Liberibacter asiaticus’ transmission},

author = {Marília Morelli and Felipe Hirayuki Takayassu and Camilla Andrade Pacheco and Patrícia Marluci Conceição and Helvécio Della Coletta Filho and Fernando Alves Azevedo},

url = {https://link.springer.com/article/10.1007/s40858-020-00371-8},

doi = {10.1007/S40858-020-00371-8/METRICS},

issn = {19832052},

year  = {2020},

date = {2020-01-01},

journal = {Tropical Plant Pathology},

volume = {45},

issue = {6},

pages = {620-625},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {Huanglongbing (HLB) is the main disease of citriculture worldwide, being mainly caused by the bacterium known as ‘Candidatus Liberibacter asiaticus’ (CLas), limited to the phloem and naturally transmitted by vector insects known as psyllids. For the production of citrus seedlings, it is necessary to use rootstocks that are propagated by seeds from fruits produced by mother plants grown in field, however being submitted to natural HLB transmission. Although there is no evidence of CLas transmission from seeds to seedlings, little is known about the effects of this bacterium on the physiological quality of seeds obtained from CLas-infected plants. Therefore, the aim of this work was to evaluate the physiological quality of seeds from ‘Rangpur’ lime and ‘Swingle’ citrumelo rootstocks, from CLas-infected plants, with symptomatic and asymptomatic branches. The parameters evaluated, in addition to fruit development in mother plants, were germination rate, tetrazolium test, electrical conductivity, seedling emergence in greenhouse, and vegetative development and molecular analysis via qPCR for the detection of bacteria (leaf petiole, fruit peduncles, roots, seed coats, and seed endosperm). The presence of CLas in plants of both rootstocks has negative impact on fruit development, as well as on the number of normal seeds. In addition, there is reduced germination and seed emergence. Although CLas was detected in different tissues (leaf petiole, fruit peduncles and seed coat, and seed embryo), no vertical bacteria transmission was detected. Results have shown that seeds from CLas-infected branches have impaired physiological quality, but without bacterial transmission from seeds to seedlings.},

keywords = {‘Rangpur’ lime, ‘Swingle’ citrumelo, bacterium, Citrus, Greening, Seeds},

pubstate = {published},

tppubtype = {article}

}