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2020
Silva, Jefferson Rangel; Alvarenga, Fausto Veiga; Boaretto, Rodrigo Marcelli; Lopes, João Roberto Spotti; Quaggio, José Antônio; Filho, Helvecio Della Coletta; Mattos, Dirceu
Em: Tropical Plant Pathology, vol. 45, iss. 6, pp. 597-610, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Bacteria acquisition, Citrus, Copper, Greening, huanglongbing, Manganese, Starch content, Zinc
@article{nokey,
title = {Following the effects of micronutrient supply in HLB-infected trees: plant responses and ‘Candidatus Liberibacter asiaticus’ acquisition by the Asian citrus psyllid},
author = {Jefferson Rangel Silva and Fausto Veiga Alvarenga and Rodrigo Marcelli Boaretto and João Roberto Spotti Lopes and José Antônio Quaggio and Helvecio Della Coletta Filho and Dirceu Mattos},
url = {https://link.springer.com/article/10.1007/s40858-020-00370-9},
doi = {10.1007/S40858-020-00370-9/FIGURES/3},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {597-610},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Despite efforts of research to establish best nutrient management practices in HLB-affected citrus orchards, there are still doubts about the contribution of such strategies to minimizing losses caused by the disease in the citrus industry. We evaluated the effects of micronutrient (Zn, Mn, and/or Cu) supply and ‘Candidatus Liberibacter asiaticus’ (CLas) infection on physiological and growth traits of sweet orange trees (Citrus sinensis (L.) Osbeck)) to understand if enhanced micronutrient supply would improve the growth of plants infected with CLas and reduce bacterial infection, as well as the acquisition of CLas by adults and nymphs of Diaphorina citri, correlated with nutritional status of trees. Plants were either grafted with buds obtained from micrografted plants (healthy, −) or buds infected with CLas (diseased plants, +). Infected plants were exposed to one of the five nutrient treatments, applied to leaf and root: (i) nilZnMnCu+: not fertilized with Cu, Mn, and Zn; (ii) Zn+: fertilized with zinc sulfate (ZnSO4.H2O); (iii) Mn+: fertilized with manganese sulfate (MnSO4.H2O); (iv) Cu+: fertilized with copper hydroxide [Cu(OH)2]; and (v) ZnMnCu+: fertilized with all three micronutrients. Likewise, healthy plants were exposed to one of the two treatments as above: (i) nilZnMnCu− or (v) ZnMnCu−. We found that CLas impairs plant biomass production regardless of nutrient treatments, especially root growth, and increases specific leaf dry weight as disease progresses because of starch accumulation. Moreover, individual supply with Zn, Mn, or Cu can mitigate such deleterious effects of HLB on starch metabolism. HLB also changes nutrient concentrations in both leaves and sap extract, regardless of nutrient treatments, although treatments do not reduce the CLas titer in plants as determined by RT-qPCR. On the contrary, micronutrients applied in combination (ZnMnCu+) can reduce the acquisition of CLas by adults and especially nymphs of D. citri, likely reducing the disease infection in citrus orchards.},
keywords = {Bacteria acquisition, Citrus, Copper, Greening, huanglongbing, Manganese, Starch content, Zinc},
pubstate = {published},
tppubtype = {article}
}
Despite efforts of research to establish best nutrient management practices in HLB-affected citrus orchards, there are still doubts about the contribution of such strategies to minimizing losses caused by the disease in the citrus industry. We evaluated the effects of micronutrient (Zn, Mn, and/or Cu) supply and ‘Candidatus Liberibacter asiaticus’ (CLas) infection on physiological and growth traits of sweet orange trees (Citrus sinensis (L.) Osbeck)) to understand if enhanced micronutrient supply would improve the growth of plants infected with CLas and reduce bacterial infection, as well as the acquisition of CLas by adults and nymphs of Diaphorina citri, correlated with nutritional status of trees. Plants were either grafted with buds obtained from micrografted plants (healthy, −) or buds infected with CLas (diseased plants, +). Infected plants were exposed to one of the five nutrient treatments, applied to leaf and root: (i) nilZnMnCu+: not fertilized with Cu, Mn, and Zn; (ii) Zn+: fertilized with zinc sulfate (ZnSO4.H2O); (iii) Mn+: fertilized with manganese sulfate (MnSO4.H2O); (iv) Cu+: fertilized with copper hydroxide [Cu(OH)2]; and (v) ZnMnCu+: fertilized with all three micronutrients. Likewise, healthy plants were exposed to one of the two treatments as above: (i) nilZnMnCu− or (v) ZnMnCu−. We found that CLas impairs plant biomass production regardless of nutrient treatments, especially root growth, and increases specific leaf dry weight as disease progresses because of starch accumulation. Moreover, individual supply with Zn, Mn, or Cu can mitigate such deleterious effects of HLB on starch metabolism. HLB also changes nutrient concentrations in both leaves and sap extract, regardless of nutrient treatments, although treatments do not reduce the CLas titer in plants as determined by RT-qPCR. On the contrary, micronutrients applied in combination (ZnMnCu+) can reduce the acquisition of CLas by adults and especially nymphs of D. citri, likely reducing the disease infection in citrus orchards.
Silva, Jefferson Rangel; Alvarenga, Fausto Veiga; Boaretto, Rodrigo Marcelli; Lopes, João Roberto Spotti; Quaggio, José Antônio; Filho, Helvecio Della Coletta; Mattos, Dirceu
Em: Tropical Plant Pathology, vol. 45, iss. 6, pp. 597-610, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Bacteria acquisition, Citrus, Copper, Greening, huanglongbing, Manganese, Starch content, Zinc
@article{nokey,
title = {Following the effects of micronutrient supply in HLB-infected trees: plant responses and ‘Candidatus Liberibacter asiaticus’ acquisition by the Asian citrus psyllid},
author = {Jefferson Rangel Silva and Fausto Veiga Alvarenga and Rodrigo Marcelli Boaretto and João Roberto Spotti Lopes and José Antônio Quaggio and Helvecio Della Coletta Filho and Dirceu Mattos},
url = {https://link.springer.com/article/10.1007/s40858-020-00370-9},
doi = {10.1007/S40858-020-00370-9/FIGURES/3},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {597-610},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Despite efforts of research to establish best nutrient management practices in HLB-affected citrus orchards, there are still doubts about the contribution of such strategies to minimizing losses caused by the disease in the citrus industry. We evaluated the effects of micronutrient (Zn, Mn, and/or Cu) supply and ‘Candidatus Liberibacter asiaticus’ (CLas) infection on physiological and growth traits of sweet orange trees (Citrus sinensis (L.) Osbeck)) to understand if enhanced micronutrient supply would improve the growth of plants infected with CLas and reduce bacterial infection, as well as the acquisition of CLas by adults and nymphs of Diaphorina citri, correlated with nutritional status of trees. Plants were either grafted with buds obtained from micrografted plants (healthy, −) or buds infected with CLas (diseased plants, +). Infected plants were exposed to one of the five nutrient treatments, applied to leaf and root: (i) nilZnMnCu+: not fertilized with Cu, Mn, and Zn; (ii) Zn+: fertilized with zinc sulfate (ZnSO4.H2O); (iii) Mn+: fertilized with manganese sulfate (MnSO4.H2O); (iv) Cu+: fertilized with copper hydroxide [Cu(OH)2]; and (v) ZnMnCu+: fertilized with all three micronutrients. Likewise, healthy plants were exposed to one of the two treatments as above: (i) nilZnMnCu− or (v) ZnMnCu−. We found that CLas impairs plant biomass production regardless of nutrient treatments, especially root growth, and increases specific leaf dry weight as disease progresses because of starch accumulation. Moreover, individual supply with Zn, Mn, or Cu can mitigate such deleterious effects of HLB on starch metabolism. HLB also changes nutrient concentrations in both leaves and sap extract, regardless of nutrient treatments, although treatments do not reduce the CLas titer in plants as determined by RT-qPCR. On the contrary, micronutrients applied in combination (ZnMnCu+) can reduce the acquisition of CLas by adults and especially nymphs of D. citri, likely reducing the disease infection in citrus orchards.},
keywords = {Bacteria acquisition, Citrus, Copper, Greening, huanglongbing, Manganese, Starch content, Zinc},
pubstate = {published},
tppubtype = {article}
}
Despite efforts of research to establish best nutrient management practices in HLB-affected citrus orchards, there are still doubts about the contribution of such strategies to minimizing losses caused by the disease in the citrus industry. We evaluated the effects of micronutrient (Zn, Mn, and/or Cu) supply and ‘Candidatus Liberibacter asiaticus’ (CLas) infection on physiological and growth traits of sweet orange trees (Citrus sinensis (L.) Osbeck)) to understand if enhanced micronutrient supply would improve the growth of plants infected with CLas and reduce bacterial infection, as well as the acquisition of CLas by adults and nymphs of Diaphorina citri, correlated with nutritional status of trees. Plants were either grafted with buds obtained from micrografted plants (healthy, −) or buds infected with CLas (diseased plants, +). Infected plants were exposed to one of the five nutrient treatments, applied to leaf and root: (i) nilZnMnCu+: not fertilized with Cu, Mn, and Zn; (ii) Zn+: fertilized with zinc sulfate (ZnSO4.H2O); (iii) Mn+: fertilized with manganese sulfate (MnSO4.H2O); (iv) Cu+: fertilized with copper hydroxide [Cu(OH)2]; and (v) ZnMnCu+: fertilized with all three micronutrients. Likewise, healthy plants were exposed to one of the two treatments as above: (i) nilZnMnCu− or (v) ZnMnCu−. We found that CLas impairs plant biomass production regardless of nutrient treatments, especially root growth, and increases specific leaf dry weight as disease progresses because of starch accumulation. Moreover, individual supply with Zn, Mn, or Cu can mitigate such deleterious effects of HLB on starch metabolism. HLB also changes nutrient concentrations in both leaves and sap extract, regardless of nutrient treatments, although treatments do not reduce the CLas titer in plants as determined by RT-qPCR. On the contrary, micronutrients applied in combination (ZnMnCu+) can reduce the acquisition of CLas by adults and especially nymphs of D. citri, likely reducing the disease infection in citrus orchards.
Martins, Paula Maria Moreira; Gong, Ting; Souza, Alessandra A.; Wood, Thomas K.
Copper Kills Escherichia coli Persister Cells Journal Article
Em: Antibiotics 2020, Vol. 9, Page 506, vol. 9, iss. 8, pp. 506, 2020, ISSN: 2079-6382.
Resumo | Links | BibTeX | Tags: Copper, persister, VBNC
@article{Martins2020,
title = {Copper Kills Escherichia coli Persister Cells},
author = {Paula Maria Moreira Martins and Ting Gong and Alessandra A. Souza and Thomas K. Wood},
url = {https://www.mdpi.com/2079-6382/9/8/506/htm https://www.mdpi.com/2079-6382/9/8/506},
doi = {10.3390/ANTIBIOTICS9080506},
issn = {2079-6382},
year = {2020},
date = {2020-01-01},
journal = {Antibiotics 2020, Vol. 9, Page 506},
volume = {9},
issue = {8},
pages = {506},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Due to their reduced metabolism, persister cells can survive most antimicrobial treatments, which usually rely on corrupting active biochemical pathways. Therefore, molecules that kill bacterial persisters should function in a metabolism-independent manner. Some anti-persister compounds have been found previously, such as the DNA-crosslinkers mitomycin C and cisplatin, but more effective and lower cost alternatives are needed. Copper alloys have been used since ancient times due to their antimicrobial properties, and they are still used in agriculture to control plant bacterial diseases. By stopping transcription with rifampicin and by treating with ampicillin to remove non-persister cells, we created a population that consists solely of Escherichia coli persister cells. Using this population of persister cells, we demonstrate that cupric compounds kill E. coli persister cells. Hence, copper ions may be used in controlling the spread of important bacterial strains that withstand treatment with conventional antimicrobials by forming persister cells.},
keywords = {Copper, persister, VBNC},
pubstate = {published},
tppubtype = {article}
}
Due to their reduced metabolism, persister cells can survive most antimicrobial treatments, which usually rely on corrupting active biochemical pathways. Therefore, molecules that kill bacterial persisters should function in a metabolism-independent manner. Some anti-persister compounds have been found previously, such as the DNA-crosslinkers mitomycin C and cisplatin, but more effective and lower cost alternatives are needed. Copper alloys have been used since ancient times due to their antimicrobial properties, and they are still used in agriculture to control plant bacterial diseases. By stopping transcription with rifampicin and by treating with ampicillin to remove non-persister cells, we created a population that consists solely of Escherichia coli persister cells. Using this population of persister cells, we demonstrate that cupric compounds kill E. coli persister cells. Hence, copper ions may be used in controlling the spread of important bacterial strains that withstand treatment with conventional antimicrobials by forming persister cells.