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2022
Merfa, Marcus Vinıcius; Fischer, Eduarda Regina; Silva, Mariana; Francisco, Carolina Sardinha; Coletta-Filho, Helvecio Della; Souza, Alessandra Alves
Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri Journal Article
Em: Phytopathology, vol. 112, iss. 1, pp. 163-172, 2022, ISSN: 19437684.
Resumo | Links | BibTeX | Tags: Bacterial pathogens, Biotechnology, Disease control and pest management, genetics
@article{Merfa2022,
title = {Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri},
author = {Marcus Vinıcius Merfa and Eduarda Regina Fischer and Mariana Silva and Carolina Sardinha Francisco and Helvecio Della Coletta-Filho and Alessandra Alves Souza},
url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-06-21-0252-FI},
doi = {10.1094/PHYTO-06-21-0252-FI/ASSET/IMAGES/LARGE/PHYTO-06-21-0252-FIF6.JPEG},
issn = {19437684},
year = {2022},
date = {2022-01-01},
journal = {Phytopathology},
volume = {112},
issue = {1},
pages = {163-172},
publisher = {American Phytopathological Society},
abstract = {Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.},
keywords = {Bacterial pathogens, Biotechnology, Disease control and pest management, genetics},
pubstate = {published},
tppubtype = {article}
}
Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.
2018
Brito, Marcos E. B.; Soares, Lauriane A. A.; Filho, Walter S. Soares; Fernandes, Pedro D.; Silva, Elaine C. B.; Sá, Francisco V. S.; Silva, Luderlândio A.
Emergence and morphophysiology of Sunki mandarin and other citrus genotypes seedlings under saline stress Journal Article
Em: Spanish Journal of Agricultural Research, vol. 16, iss. 1, pp. e0801-e0801, 2018, ISSN: 2171-9292.
Resumo | Links | BibTeX | Tags: agriculture, breeding, Citrus spp., diseases, economics, fertilization, genetic resources, genetics, growth, irrigation, livestock, pests, photosynthesis, physiology, reproduction, rootstock, Salinity, soils, water manage, weeds
@article{Brito2018,
title = {Emergence and morphophysiology of Sunki mandarin and other citrus genotypes seedlings under saline stress},
author = {Marcos E. B. Brito and Lauriane A. A. Soares and Walter S. Soares Filho and Pedro D. Fernandes and Elaine C. B. Silva and Francisco V. S. Sá and Luderlândio A. Silva},
url = {https://revistas.inia.es/index.php/sjar/article/view/9400/3932 https://revistas.inia.es/index.php/sjar/article/view/9400},
doi = {10.5424/SJAR/2018161-9400},
issn = {2171-9292},
year = {2018},
date = {2018-01-01},
journal = {Spanish Journal of Agricultural Research},
volume = {16},
issue = {1},
pages = {e0801-e0801},
publisher = {Ministerio de Agricultura Pesca y Alimentacion},
abstract = {The scarcity of good quality water is a limiting factor for irrigated agriculture, especially in arid and semiarid regions, where water sources generally contain high concentrations of ions. In such conditions, it is essential to cultivate genotypes with economic potential and tolerance to salinity. Considering the importance of citrus and the need to identify genetic materials that adapt to saline stress, this study evaluates the salinity tolerance of 10 genotypes with rootstock potential. For the irrigation water, five levels of electrical conductivity (ECw: 0.8, 1.6, 2.4, 3.2 and 4.0 dS/m at 25 °C) were used in a randomized block design with three replications, and seed germination and growth variables, as well as physiological plant parameters, were evaluated. The germination rate of the hybrid TSKC × CTARG – 019 was the best under salt conditions, as it was the genotype with the highest tolerance to salinity in the germination stage. Salinity reduced the growth of the citrus genotypes, with the possibility of using water with EC of up to 1.6 dS/m in the rootstock formation stage. Salt stress affected the photosynthetic rate of the genotypes ‘San Diego’ citrandarin and TSKC × CTSW – 018 by reducing the stomatal conductance, restricting the diffusion of CO2 into the substomatal camera. The genotypes TSKC × CTARG – 019, TSKC × CTTR – 012 and TSKC × TRBK – 007 have the greatest potential for growth and photosynthetic apparatus efficiency when subjected to salinity.},
keywords = {agriculture, breeding, Citrus spp., diseases, economics, fertilization, genetic resources, genetics, growth, irrigation, livestock, pests, photosynthesis, physiology, reproduction, rootstock, Salinity, soils, water manage, weeds},
pubstate = {published},
tppubtype = {article}
}
The scarcity of good quality water is a limiting factor for irrigated agriculture, especially in arid and semiarid regions, where water sources generally contain high concentrations of ions. In such conditions, it is essential to cultivate genotypes with economic potential and tolerance to salinity. Considering the importance of citrus and the need to identify genetic materials that adapt to saline stress, this study evaluates the salinity tolerance of 10 genotypes with rootstock potential. For the irrigation water, five levels of electrical conductivity (ECw: 0.8, 1.6, 2.4, 3.2 and 4.0 dS/m at 25 °C) were used in a randomized block design with three replications, and seed germination and growth variables, as well as physiological plant parameters, were evaluated. The germination rate of the hybrid TSKC × CTARG – 019 was the best under salt conditions, as it was the genotype with the highest tolerance to salinity in the germination stage. Salinity reduced the growth of the citrus genotypes, with the possibility of using water with EC of up to 1.6 dS/m in the rootstock formation stage. Salt stress affected the photosynthetic rate of the genotypes ‘San Diego’ citrandarin and TSKC × CTSW – 018 by reducing the stomatal conductance, restricting the diffusion of CO2 into the substomatal camera. The genotypes TSKC × CTARG – 019, TSKC × CTTR – 012 and TSKC × TRBK – 007 have the greatest potential for growth and photosynthetic apparatus efficiency when subjected to salinity.