Publicações

@article{Behlau2021,

title = {Chlorine dioxide, peroxyacetic acid, and calcium oxychloride for post-harvest decontamination of citrus fruit against Xanthomonas citri subsp. citri, causal agent of citrus canker},

author = {Franklin Behlau and Alexandre Paloschi and Tamiris G. S. Marin and Talita A. Santos and Henrique Ferreira and Lenice M. do Nascimento},

doi = {10.1016/J.CROPRO.2021.105679},

issn = {0261-2194},

year  = {2021},

date = {2021-01-01},

journal = {Crop Protection},

volume = {146},

pages = {105679},

publisher = {Elsevier},

abstract = {After a new regulation on the control of citrus canker (Xanthomonas citri subsp. citri) came into force in Brazil in 2017, the post-harvest sanitization of citrus fruit became mandatory to prevent dissemination of the causal pathogen and enable the commercial trade of citrus to other states and countries. Although several sanitizers are available worldwide for decontamination of fresh produce, only sodium hypochlorite is legally required by the country for decontamination of citrus fruit traded with other states and countries against X. citri in the packing house. Thus, the objectives of this study were to assess the efficiency of chlorine dioxide, peracetic acid, and calcium oxychloride as alternative sanitizers and exposure times of 1 and 2 min for the post-harvest decontamination of citrus fruit against X. citri. Sodium hypochlorite, the legally required standard, was used as control. The sanitizers were evaluated regarding the capacity to eliminate live X. citri in solution immediately or 1 h after exposure to the products and the efficiency in disinfesting artificially and naturally contaminated lime fruit. An additional assessment was carried out to determine the infective potential of X. citri suspensions at different concentrations in order to evaluate the risk of disease dissemination by the remaining bacterial population on the fruit following treatment. All the assessed sanitizers were able to completely eliminate X. citri in suspension immediately after treatment. In artificially and naturally contaminated fruit, the sanitizers promoted a significant reduction of 2.4–2.8 and 1.1 to 1.5 log10 cfu/mL, respectively, in the population of live bacteria when compared to the untreated control. In these experiments, the amount of X. citri that remained on the fruit ranged from 0.5 to 1.0 and 0.1 to 0.3 log10 cfu/mL, respectively, regardless of the exposure time. The labeled concentrations and treatment periods assessed effectively decontaminated fruit with X. citri and demonstrated little or no risk of pathogen spread from treated fruit.},

keywords = {Lemon, Lime, Orange, Packing house, Sanitization, Sodium hypochlorite},

pubstate = {published},

tppubtype = {article}

}

@article{Zamuner2020,

title = {A cinnamaldehyde-based formulation as an alternative to sodium hypochlorite for post-harvest decontamination of citrus fruit},

author = {Caio F. C. Zamuner and Guilherme Dilarri and Lucia C. Bonci and Luiz L. Saldanha and Franklin Behlau and Tamiris G. S. Marin and Daiane C. Sass and Mauricio Bacci and Henrique Ferreira},

url = {https://link.springer.com/article/10.1007/s40858-020-00338-9},

doi = {10.1007/S40858-020-00338-9/METRICS},

issn = {19832052},

year  = {2020},

date = {2020-01-01},

journal = {Tropical Plant Pathology},

volume = {45},

issue = {6},

pages = {701-709},

publisher = {Springer Science and Business Media Deutschland GmbH},

abstract = {According to the most recent regulation, published in 2018, areas or states of Brazil where citrus canker is endemic are no longer obliged to eradicate citrus trees affected by the disease as in the past 60 years. Instead, growers have to adopt a set of control measures, such as copper sprays, windbreaks, and control of the citrus leaf miner to minimize the impact of the disease on fruit quality and yield. Another important change was that all fresh Fruit commercialized out of the state of origin and to other countries have to be sanitized against the canker-causing bacterium Xanthomonas citri subsp. citri (X. citri). Initially, sodium hypochlorite (NaOCl) was the only product allowed in Brazil by the referred legislation. Recently, this bactericide was prohibited to be used on fresh fruit shipped to the European Union and replaced by eugenol at 2%. Although effective, NaOCl may damage fruit skin, cause corrosion of packing house equipment and react with organic matter, which generates noxious by products. Here, we evaluated an alternative to NaOCl known as PosFruit. GC/MS and 1H NMR chemical analyses showed that Posfruit contains both cinnamaldehyde isomers, with the trans being present in larger quantities. We showed that PosFruit was as effective as NaOCl to eliminate X. citri from citrus fruit artificially contaminated with the bacterium. In a pilot sanitization line, treatment with 2% PosFruit reduced the X. citri population on contaminated fruit by 4 log10 CFU/mL. Furthermore, we detect neither the natural resistance of X. citri to PosFruit nor the persistence of the bacterium following progressive exposure to the product, which indicates that the product has multi-target action. PosFruit is a plant fortifier, residue-free, and efficient alternative to NaOCl for post-harvest decontamination of citrus fruit against X. citri.},

keywords = {Citrus canker, Fresh fruit, Sanitization},

pubstate = {published},

tppubtype = {article}

}