Publications

@article{Sousa2022,

title = {Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations},

author = {Andressa Rodrigues Oliveira Sousa and Edson Mario Andrade Silva and Maurício Antônio Coelho Filho and Marcio Gilberto Cardoso Costa and Walter Santos Soares Filho and Fabienne Micheli and Biancaelena Maserti and Abelmon Silva Gesteira},

doi = {10.1016/J.SCIENTA.2021.110490},

issn = {0304-4238},

year  = {2022},

date = {2022-01-01},

journal = {Scientia Horticulturae},

volume = {292},

pages = {110490},

publisher = {Elsevier},

abstract = {Drought is one of the most destructive environmental challenges for agriculture, and citrus trees are cultivated in semiarid areas. The current study used physiological and untarget mass spectrometry analysis to assess the differential response to drought and rehydration in leaves and roots of three scion/rootstock combinations in which the same scion Valencia Orange was grafted onto three different rootstocks, namely, ‘Rangpur Lime’, ‘Sunki Maravilha’ and ‘Sunki Tropical’, known to have a different response to drought. The main question to be answer was whether the soil disease tolerant ‘Sunki Tropical’ could be used as alternative to the drought tolerant/disease susceptible rootstock ‘Rangpur lime’ to cultivate citrus in semiarid areas. Thirty-seven metabolites in leaves and forty-six in roots, mainly amino acids, as well as a few carbohydrates and organic acids were found differentially expressed after drought and/or rehydration in the three combinations. Overall, the results demonstrated that the three scion/rootstock combinations responded differently at metabolite levels to water changes. The few leaf metabolites found in the scion grafted on ‘Rangpur Lime’ and ‘Sunki Maravilha’ support the respective drought avoidance and tolerance strategy, already observed in those rootstocks.‘Rangur Lime’ and ‘Sunki Tropical’ shared at roots levels several common drought response mechanisms. Moreover, the high number of differential metabolites triggered in the scion by 'Sunki Tropical' seems to be useful for overcoming the drought effects without depleting the fruit quality. From our data, we argue that ‘Sunki Tropical’ is a drought tolerant genotypes that could be used as alternative to ‘Ranpur Lime’ to sustain citrus production in semiarid areas.},

keywords = {Amino acids, Carbohydrates, Citrus, drought, Metabolic profile, rootstock},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},

author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},

doi = {10.1016/J.SCIENTA.2020.109838},

issn = {0304-4238},

year  = {2021},

date = {2021-01-01},

journal = {Scientia Horticulturae},

volume = {277},

pages = {109838},

publisher = {Elsevier},

abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},

keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},

author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},

doi = {10.1016/J.SCIENTA.2020.109838},

issn = {0304-4238},

year  = {2021},

date = {2021-01-01},

journal = {Scientia Horticulturae},

volume = {277},

pages = {109838},

publisher = {Elsevier},

abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},

keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},

pubstate = {published},

tppubtype = {article}

}

@article{Almeida2018,

title = {Water deficit increases the frequency of hybrid citrus with polyembryonic female parents},

author = {Lucas Aragão Hora Almeida and Dayse Drielly Souza Santana-Vieira and Nayara Almeida Santos and Ivan Schuster and Walter Santos Soares Filho and Maurício Antônio Coelho Filho and Abelmon Silva Gesteira},

url = {http://www.scielo.br/j/cbab/a/xDqvSnnrPRKB35CCNQ5N6Rw/?lang=en},

doi = {10.1590/1984-70332018V18N1A7},

issn = {1518-7853},

year  = {2018},

date = {2018-01-01},

journal = {Crop Breeding and Applied Biotechnology},

volume = {18},

issue = {1},

pages = {47-54},

publisher = {Crop Breeding and Applied Biotechnology},

abstract = {Abstract The aim of this study was to develop a methodology that uses water deficit induction to increase the frequency of hybrids in Citrus varieties that have a high and an intermediate level of polyembryony, respectively. The experiment examined the ‘Sunki Tropical’ mandarin and ‘Rangpur Santa Cruz’ lime genotypes at three water stress intensities: 20, 40 and 100% of the pot capacity (PC). The generated fruits were harvested, and the seeds were counted. After germination, the number of nucellar and hybrid seedlings was determined. For ‘Rangpur Santa Cruz’ lime genotype, the highest hybrid frequency generated was 64.2%, which occurred at a PC of 40% and exceeded by 12% that occurring at a PC of 100%. For ‘Sunki Tropical’ mandarin, the highest observed hybrid frequency was 21.75%, which occurred at a PC of 20% and was more than double the hybrid frequency of 8.57% observed at 100% of the PC.},

keywords = {apomixis, Citrus spp, drought, Plant breeding, rootstock},

pubstate = {published},

tppubtype = {article}

}

@article{Neves2017,

title = {Recurrent water deficit causes epigenetic and hormonal changes in citrus plants},

author = {Diana Matos Neves and Lucas Aragão Da Hora Almeida and Dayse Drielly Souza Santana-Vieira and Luciano Freschi and Claudia Fortes Ferreira and Walter Dos Santos Soares Filho and Marcio Gilberto Cardoso Costa and Fabienne Micheli and Maurício Antônio Coelho Filho and Abelmon Da Silva Gesteira},

url = {https://www.nature.com/articles/s41598-017-14161-x},

doi = {10.1038/s41598-017-14161-x},

issn = {2045-2322},

year  = {2017},

date = {2017-01-01},

journal = {Scientific Reports 2017 7:1},

volume = {7},

issue = {1},

pages = {1-11},

publisher = {Nature Publishing Group},

abstract = {The present study evaluated the physiological, molecular and hormonal parameters from scion/rootstock interaction of citrus plants during recurrent water deficit. Responses of the Valencia (VO) scion variety grafted on two rootstocks with different soil water extraction capacities, Rangpur Lime (RL) and Sunki Maravilha (SM), during three successive periods of water deficit: plants exposed to a single episode of water deficit (WD1) and plants exposed to two (WD2) and three (WD3) recurrent periods of WD were compared. The combinations VO/RL and VO/SM presented polymorphic alterations of epigenetic marks and hormonal (i.e. abscisic acid, auxins and salicylicacid) profiles, which were particularly prominent when VO/SM plantswere exposed toWD3 treatment. Upon successive drought events, the VO/SM combination presented acclimatization characteristics that enable higher tolerance to water deficit by increasing transpiration (E), stomatal conductance (g s ) and photosynthetic rate (A), which in turn may have facilitated the whole plant survival. Besides providing comprehensive data on the scion/rootstock interactions upon successive stress events, this study brings the first dataset suggesting that epigenetic alterations in citrus plants triggered by recurrent water deficit lead to improved drought tolerance in this crop species.},

keywords = {drought, Plant physiology},

pubstate = {published},

tppubtype = {article}

}