Publications

@article{nokey,

title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},

author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},

doi = {10.1016/J.SCIENTA.2020.109838},

issn = {0304-4238},

year  = {2021},

date = {2021-01-01},

journal = {Scientia Horticulturae},

volume = {277},

pages = {109838},

publisher = {Elsevier},

abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},

keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},

author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},

doi = {10.1016/J.SCIENTA.2020.109838},

issn = {0304-4238},

year  = {2021},

date = {2021-01-01},

journal = {Scientia Horticulturae},

volume = {277},

pages = {109838},

publisher = {Elsevier},

abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},

keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},

pubstate = {published},

tppubtype = {article}

}

@article{Arena2020,

title = {Plant Immune System Activation Upon Citrus Leprosis Virus C Infection Is Mimicked by the Ectopic Expression of the P61 Viral Protein},

author = {Gabriella D. Arena and Pedro Luis Ramos-González and Bryce W. Falk and Clare L. Casteel and Juliana Freitas-Astúa and Marcos A. Machado},

doi = {10.3389/FPLS.2020.01188/BIBTEX},

issn = {1664462X},

year  = {2020},

date = {2020-01-01},

journal = {Frontiers in Plant Science},

volume = {11},

pages = {1188},

publisher = {Frontiers Media S.A.},

abstract = {Citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses.},

keywords = {Arabidopsis thaliana, Cilevirus, hypersensitive response, Jasmonic acid, Nicotiana benthamiana, plant–virus interaction, RNA-Seq, Salicylic acid},

pubstate = {published},

tppubtype = {article}

}

@article{Arena2018,

title = {Making a better home: modulation of plant defensive response by brevipalpus mites},

author = {Gabriella D. Arena and Pedro L. Ramos-González and Luana A. Rogerio and Marcelo Ribeiro-Alves and Clare L. Casteel and Juliana Freitas-Astúa and Marcos A. Machado},

doi = {10.3389/FPLS.2018.01147/BIBTEX},

issn = {1664462X},

year  = {2018},

date = {2018-01-01},

journal = {Frontiers in Plant Science},

volume = {9},

pages = {1147},

publisher = {Frontiers Media S.A.},

abstract = {False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growth-defense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the non-infested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JA-mediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway.},

keywords = {Cross-talk, Defense pathways, Jasmonic acid, Plant hormones, Plant–herbivore interaction, RNA-Seq, Salicylic acid, Tetranychus},

pubstate = {published},

tppubtype = {article}

}