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2022
Sousa, Andressa Rodrigues Oliveira; Ribas, Rogério Ferreira; Filho, Mauricio Antônio Coelho; Freschi, Luciano; Ferreira, Claudia Fortes; Filho, Walter Santos Soares; Pérez-Molina, Junior Pastor; Gesteira, Abelmon Silva
Drought tolerance memory transmission by citrus buds Journal Article
In: Plant Science, vol. 320, pp. 111292, 2022, ISSN: 0168-9452.
Abstract | Links | BibTeX | Tags: Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit
@article{nokey,
title = {Drought tolerance memory transmission by citrus buds},
author = {Andressa Rodrigues Oliveira Sousa and Rogério Ferreira Ribas and Mauricio Antônio Coelho Filho and Luciano Freschi and Claudia Fortes Ferreira and Walter Santos Soares Filho and Junior Pastor Pérez-Molina and Abelmon Silva Gesteira},
doi = {10.1016/J.PLANTSCI.2022.111292},
issn = {0168-9452},
year = {2022},
date = {2022-01-01},
journal = {Plant Science},
volume = {320},
pages = {111292},
publisher = {Elsevier},
abstract = {Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.},
keywords = {Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit},
pubstate = {published},
tppubtype = {article}
}
Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.
Sousa, Andressa Rodrigues Oliveira; Ribas, Rogério Ferreira; Filho, Mauricio Antônio Coelho; Freschi, Luciano; Ferreira, Claudia Fortes; Filho, Walter Santos Soares; Pérez-Molina, Junior Pastor; Gesteira, Abelmon Silva
Drought tolerance memory transmission by citrus buds Journal Article
In: Plant Science, vol. 320, pp. 111292, 2022, ISSN: 0168-9452.
Abstract | Links | BibTeX | Tags: Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit
@article{nokey,
title = {Drought tolerance memory transmission by citrus buds},
author = {Andressa Rodrigues Oliveira Sousa and Rogério Ferreira Ribas and Mauricio Antônio Coelho Filho and Luciano Freschi and Claudia Fortes Ferreira and Walter Santos Soares Filho and Junior Pastor Pérez-Molina and Abelmon Silva Gesteira},
doi = {10.1016/J.PLANTSCI.2022.111292},
issn = {0168-9452},
year = {2022},
date = {2022-01-01},
journal = {Plant Science},
volume = {320},
pages = {111292},
publisher = {Elsevier},
abstract = {Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.},
keywords = {Abiotic stress, Citrus, Epigenetics, Photosynthetic performance, water deficit},
pubstate = {published},
tppubtype = {article}
}
Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely ‘Rangpur Santa Cruz’ lime, ‘Sunki Maravilha’ mandarin and ‘Sunki Tropical’ mandarin, in combination with ‘Valencia’ orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from ‘Valencia’ orange were grafted onto ‘Swingle’ citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially ‘Valencia’ buds grafted onto ‘Sunki Maravilha’, with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants’ capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.
Sousa, Andressa Rodrigues Oliveira; Silva, Edson Mario Andrade; Filho, Maurício Antônio Coelho; Costa, Marcio Gilberto Cardoso; Filho, Walter Santos Soares; Micheli, Fabienne; Maserti, Biancaelena; Gesteira, Abelmon Silva
Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations Journal Article
In: Scientia Horticulturae, vol. 292, pp. 110490, 2022, ISSN: 0304-4238.
Abstract | Links | BibTeX | Tags: Amino acids, Carbohydrates, Citrus, drought, Metabolic profile, rootstock
@article{Sousa2022,
title = {Metabolic responses to drought stress and rehydration in leaves and roots of three Citrus scion/rootstock combinations},
author = {Andressa Rodrigues Oliveira Sousa and Edson Mario Andrade Silva and Maurício Antônio Coelho Filho and Marcio Gilberto Cardoso Costa and Walter Santos Soares Filho and Fabienne Micheli and Biancaelena Maserti and Abelmon Silva Gesteira},
doi = {10.1016/J.SCIENTA.2021.110490},
issn = {0304-4238},
year = {2022},
date = {2022-01-01},
journal = {Scientia Horticulturae},
volume = {292},
pages = {110490},
publisher = {Elsevier},
abstract = {Drought is one of the most destructive environmental challenges for agriculture, and citrus trees are cultivated in semiarid areas. The current study used physiological and untarget mass spectrometry analysis to assess the differential response to drought and rehydration in leaves and roots of three scion/rootstock combinations in which the same scion Valencia Orange was grafted onto three different rootstocks, namely, ‘Rangpur Lime’, ‘Sunki Maravilha’ and ‘Sunki Tropical’, known to have a different response to drought. The main question to be answer was whether the soil disease tolerant ‘Sunki Tropical’ could be used as alternative to the drought tolerant/disease susceptible rootstock ‘Rangpur lime’ to cultivate citrus in semiarid areas. Thirty-seven metabolites in leaves and forty-six in roots, mainly amino acids, as well as a few carbohydrates and organic acids were found differentially expressed after drought and/or rehydration in the three combinations. Overall, the results demonstrated that the three scion/rootstock combinations responded differently at metabolite levels to water changes. The few leaf metabolites found in the scion grafted on ‘Rangpur Lime’ and ‘Sunki Maravilha’ support the respective drought avoidance and tolerance strategy, already observed in those rootstocks.‘Rangur Lime’ and ‘Sunki Tropical’ shared at roots levels several common drought response mechanisms. Moreover, the high number of differential metabolites triggered in the scion by 'Sunki Tropical' seems to be useful for overcoming the drought effects without depleting the fruit quality. From our data, we argue that ‘Sunki Tropical’ is a drought tolerant genotypes that could be used as alternative to ‘Ranpur Lime’ to sustain citrus production in semiarid areas.},
keywords = {Amino acids, Carbohydrates, Citrus, drought, Metabolic profile, rootstock},
pubstate = {published},
tppubtype = {article}
}
Drought is one of the most destructive environmental challenges for agriculture, and citrus trees are cultivated in semiarid areas. The current study used physiological and untarget mass spectrometry analysis to assess the differential response to drought and rehydration in leaves and roots of three scion/rootstock combinations in which the same scion Valencia Orange was grafted onto three different rootstocks, namely, ‘Rangpur Lime’, ‘Sunki Maravilha’ and ‘Sunki Tropical’, known to have a different response to drought. The main question to be answer was whether the soil disease tolerant ‘Sunki Tropical’ could be used as alternative to the drought tolerant/disease susceptible rootstock ‘Rangpur lime’ to cultivate citrus in semiarid areas. Thirty-seven metabolites in leaves and forty-six in roots, mainly amino acids, as well as a few carbohydrates and organic acids were found differentially expressed after drought and/or rehydration in the three combinations. Overall, the results demonstrated that the three scion/rootstock combinations responded differently at metabolite levels to water changes. The few leaf metabolites found in the scion grafted on ‘Rangpur Lime’ and ‘Sunki Maravilha’ support the respective drought avoidance and tolerance strategy, already observed in those rootstocks.‘Rangur Lime’ and ‘Sunki Tropical’ shared at roots levels several common drought response mechanisms. Moreover, the high number of differential metabolites triggered in the scion by 'Sunki Tropical' seems to be useful for overcoming the drought effects without depleting the fruit quality. From our data, we argue that ‘Sunki Tropical’ is a drought tolerant genotypes that could be used as alternative to ‘Ranpur Lime’ to sustain citrus production in semiarid areas.
2021
Silva, Danielle Fernandes; Amaral, Jéssica Cristina; Carlos, Rose Maria; Ferreira, Antonio Gilberto; Forim, Moacir Rossi; Fernandes, João Batista; Silva, Maria Fátima; Filho, Helvécio Della Coletta; Souza, Alessandra Alves
In: Talanta, vol. 225, pp. 122040, 2021, ISSN: 0039-9140.
Abstract | Links | BibTeX | Tags: Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa
@article{nokey,
title = {Octahedral ruthenium and magnesium naringenin 5-alkoxide complexes: NMR analysis of diastereoisomers and in-vivo antibacterial activity against Xylella fastidiosa},
author = {Danielle Fernandes Silva and Jéssica Cristina Amaral and Rose Maria Carlos and Antonio Gilberto Ferreira and Moacir Rossi Forim and João Batista Fernandes and Maria Fátima Silva and Helvécio Della Coletta Filho and Alessandra Alves Souza},
doi = {10.1016/J.TALANTA.2020.122040},
issn = {0039-9140},
year = {2021},
date = {2021-01-01},
journal = {Talanta},
volume = {225},
pages = {122040},
publisher = {Elsevier},
abstract = {Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.},
keywords = {Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.
Carvalho, Hélio Wilson Lemos; Carvalho, Luciana Marques; Teodoro, Adenir Vieira; Barros, Inácio; Girardi, Eduardo Augusto; Filho, Walter Dos Santos Soares; Passos, Orlando Sampaio
Yield, fruit quality, and survival of 'Pêra' sweet orange on eight rootstocks in tropical cohesive soils Journal Article
In: Pesquisa Agropecuária Brasileira, vol. 56, pp. 1-9, 2021, ISSN: 0100-204X.
Abstract | Links | BibTeX | Tags: citrandarins, Citrus, coastal tablelands, Poncirus trifoliata, rainfed cultivation, scion/rootstocks combinations, tabuleiros costeiros
@article{nokey,
title = {Yield, fruit quality, and survival of 'Pêra' sweet orange on eight rootstocks in tropical cohesive soils},
author = {Hélio Wilson Lemos Carvalho and Luciana Marques Carvalho and Adenir Vieira Teodoro and Inácio Barros and Eduardo Augusto Girardi and Walter Dos Santos Soares Filho and Orlando Sampaio Passos},
url = {http://www.scielo.br/j/pab/a/dhgGG3cZNXxTsfT4xpCRGkr/?lang=en},
doi = {10.1590/S1678-3921.PAB2021.V56.02151},
issn = {0100-204X},
year = {2021},
date = {2021-01-01},
journal = {Pesquisa Agropecuária Brasileira},
volume = {56},
pages = {1-9},
publisher = {Embrapa Secretaria de Pesquisa e Desenvolvimento, Pesquisa Agropecuária Brasileira},
abstract = {Abstract: The objective of this work was to determine the influence of eight rootstocks on the yield, fruit quality, and survival of 'Pêra CNPMF D6' sweet orange (Citrus sinensis) trees grown under rainfed conditions in a cohesive soil of the Brazilian Northeast. In 2014-2018, the yield, fruits, and survival of trees planted in 1997 were evaluated in a randomized complete block design. Yield was assessed using annual fruit production, yield efficiency, and the alternate bearing index. Tree survival was considered the percentage of plants that remained alive until 2018. Fruit quality was evaluated by physicochemical attributes. 'Cravo' confirmed its good yield performance. Despite the lower number of trees, the 'Mazoe' rough lemon rootstock induced the highest fruit yield. The 'Cravo' x 'Cleópatra' hybrid and 'Volkameriano' lemon favored the highest trees survival, whereas 'Indio' citrandarin and 'Cravo' lime resulted in intermediate levels. The 'San Diego' and 'Riverside' citrandarins induced shorter tree with a high yield efficiency and a higher alternate bearing index, respectively. Fruits with less juice and mass were induced, respectively, by 'Riverside' citrandarin and 'Volkameriano' lemon. It can be concluded that the 'Mazoe' rootstock induces a higher fruit yield and 'Cravo' x 'Cleópatra' and 'Volkameriano', a greater survival. In addition, all rootstocks, except 'Riverside' and 'Volkameriano', induce fruit that meet the minimum quality requirements.},
keywords = {citrandarins, Citrus, coastal tablelands, Poncirus trifoliata, rainfed cultivation, scion/rootstocks combinations, tabuleiros costeiros},
pubstate = {published},
tppubtype = {article}
}
Abstract: The objective of this work was to determine the influence of eight rootstocks on the yield, fruit quality, and survival of 'Pêra CNPMF D6' sweet orange (Citrus sinensis) trees grown under rainfed conditions in a cohesive soil of the Brazilian Northeast. In 2014-2018, the yield, fruits, and survival of trees planted in 1997 were evaluated in a randomized complete block design. Yield was assessed using annual fruit production, yield efficiency, and the alternate bearing index. Tree survival was considered the percentage of plants that remained alive until 2018. Fruit quality was evaluated by physicochemical attributes. 'Cravo' confirmed its good yield performance. Despite the lower number of trees, the 'Mazoe' rough lemon rootstock induced the highest fruit yield. The 'Cravo' x 'Cleópatra' hybrid and 'Volkameriano' lemon favored the highest trees survival, whereas 'Indio' citrandarin and 'Cravo' lime resulted in intermediate levels. The 'San Diego' and 'Riverside' citrandarins induced shorter tree with a high yield efficiency and a higher alternate bearing index, respectively. Fruits with less juice and mass were induced, respectively, by 'Riverside' citrandarin and 'Volkameriano' lemon. It can be concluded that the 'Mazoe' rootstock induces a higher fruit yield and 'Cravo' x 'Cleópatra' and 'Volkameriano', a greater survival. In addition, all rootstocks, except 'Riverside' and 'Volkameriano', induce fruit that meet the minimum quality requirements.
Santos, Ariana Silva; Andrade, Edson Mário; Sousa, Andressa Rodrigues Oliveira; Costa, Marcos Mota Carmo; Togawa, Roberto; Grynberg, Priscila; Pirovani, Carlos Priminho; Oliveira, Saulo Alves Silva; Filho, Walter Santos Soares; Filho, Mauricio Antônio Coelho; Gesteira, Abelmon Silva; Ferreira, Claudia Fortes
Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies Journal Article
In: Scientia Horticulturae, vol. 277, pp. 109838, 2021, ISSN: 0304-4238.
Abstract | Links | BibTeX | Tags: Citrus, drought, RNA-Seq, root, Sunki, Transcriptome
@article{nokey,
title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},
author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},
doi = {10.1016/J.SCIENTA.2020.109838},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {277},
pages = {109838},
publisher = {Elsevier},
abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},
keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},
pubstate = {published},
tppubtype = {article}
}
Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.
Amorim, Maurício Silva; Toyosumi, Iumi Silva; Lopes, Wilson Amorim; Camillo, Luciana Rodrigues; Ferreira, Laiane Nascimento; Junior, Dilson Souza Rocha; Filho, Walter Santos Soares; Gesteira, Abelmon Silva; Costa, Marcio Gilberto Cardoso; Filho, Maurício Antônio Coelho
In: https://doi.org/10.1080/14620316.2021.1900750, vol. 96, iss. 5, pp. 663-672, 2021, ISSN: 14620316.
Abstract | Links | BibTeX | Tags: Citrus, Fruit quality, Gene expression, irrigation, water deficit, Yield
@article{Amorim2021,
title = {Partial rootzone drying and regulated deficit irrigation can be used as water-saving strategies without compromising fruit yield and quality in tropically grown sweet orange},
author = {Maurício Silva Amorim and Iumi Silva Toyosumi and Wilson Amorim Lopes and Luciana Rodrigues Camillo and Laiane Nascimento Ferreira and Dilson Souza Rocha Junior and Walter Santos Soares Filho and Abelmon Silva Gesteira and Marcio Gilberto Cardoso Costa and Maurício Antônio Coelho Filho},
url = {https://www.tandfonline.com/doi/abs/10.1080/14620316.2021.1900750},
doi = {10.1080/14620316.2021.1900750},
issn = {14620316},
year = {2021},
date = {2021-01-01},
journal = {https://doi.org/10.1080/14620316.2021.1900750},
volume = {96},
issue = {5},
pages = {663-672},
publisher = {Taylor & Francis},
abstract = {We examined the effects of partial rootzone drying (PRD) and regulated deficit irrigation (RDI) on some physiological and agronomical traits in tropically grown sweet orange (Citrus sinensis (L.) O...},
keywords = {Citrus, Fruit quality, Gene expression, irrigation, water deficit, Yield},
pubstate = {published},
tppubtype = {article}
}
We examined the effects of partial rootzone drying (PRD) and regulated deficit irrigation (RDI) on some physiological and agronomical traits in tropically grown sweet orange (Citrus sinensis (L.) O...
Silva, Danielle Fernandes; Amaral, Jéssica Cristina; Carlos, Rose Maria; Ferreira, Antonio Gilberto; Forim, Moacir Rossi; Fernandes, João Batista; Silva, Maria Fátima; Filho, Helvécio Della Coletta; Souza, Alessandra Alves
In: Talanta, vol. 225, pp. 122040, 2021, ISSN: 0039-9140.
Abstract | Links | BibTeX | Tags: Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa
@article{nokey,
title = {Octahedral ruthenium and magnesium naringenin 5-alkoxide complexes: NMR analysis of diastereoisomers and in-vivo antibacterial activity against Xylella fastidiosa},
author = {Danielle Fernandes Silva and Jéssica Cristina Amaral and Rose Maria Carlos and Antonio Gilberto Ferreira and Moacir Rossi Forim and João Batista Fernandes and Maria Fátima Silva and Helvécio Della Coletta Filho and Alessandra Alves Souza},
doi = {10.1016/J.TALANTA.2020.122040},
issn = {0039-9140},
year = {2021},
date = {2021-01-01},
journal = {Talanta},
volume = {225},
pages = {122040},
publisher = {Elsevier},
abstract = {Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.},
keywords = {Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.
Carvalho, Hélio Wilson Lemos; Carvalho, Luciana Marques; Teodoro, Adenir Vieira; Barros, Inácio; Girardi, Eduardo Augusto; Filho, Walter Dos Santos Soares; Passos, Orlando Sampaio
Yield, fruit quality, and survival of 'Pêra' sweet orange on eight rootstocks in tropical cohesive soils Journal Article
In: Pesquisa Agropecuária Brasileira, vol. 56, pp. 1-9, 2021, ISSN: 0100-204X.
Abstract | Links | BibTeX | Tags: citrandarins, Citrus, coastal tablelands, Poncirus trifoliata, rainfed cultivation, scion/rootstocks combinations, tabuleiros costeiros
@article{nokey,
title = {Yield, fruit quality, and survival of 'Pêra' sweet orange on eight rootstocks in tropical cohesive soils},
author = {Hélio Wilson Lemos Carvalho and Luciana Marques Carvalho and Adenir Vieira Teodoro and Inácio Barros and Eduardo Augusto Girardi and Walter Dos Santos Soares Filho and Orlando Sampaio Passos},
url = {http://www.scielo.br/j/pab/a/dhgGG3cZNXxTsfT4xpCRGkr/?lang=en},
doi = {10.1590/S1678-3921.PAB2021.V56.02151},
issn = {0100-204X},
year = {2021},
date = {2021-01-01},
journal = {Pesquisa Agropecuária Brasileira},
volume = {56},
pages = {1-9},
publisher = {Embrapa Secretaria de Pesquisa e Desenvolvimento, Pesquisa Agropecuária Brasileira},
abstract = {Abstract: The objective of this work was to determine the influence of eight rootstocks on the yield, fruit quality, and survival of 'Pêra CNPMF D6' sweet orange (Citrus sinensis) trees grown under rainfed conditions in a cohesive soil of the Brazilian Northeast. In 2014-2018, the yield, fruits, and survival of trees planted in 1997 were evaluated in a randomized complete block design. Yield was assessed using annual fruit production, yield efficiency, and the alternate bearing index. Tree survival was considered the percentage of plants that remained alive until 2018. Fruit quality was evaluated by physicochemical attributes. 'Cravo' confirmed its good yield performance. Despite the lower number of trees, the 'Mazoe' rough lemon rootstock induced the highest fruit yield. The 'Cravo' x 'Cleópatra' hybrid and 'Volkameriano' lemon favored the highest trees survival, whereas 'Indio' citrandarin and 'Cravo' lime resulted in intermediate levels. The 'San Diego' and 'Riverside' citrandarins induced shorter tree with a high yield efficiency and a higher alternate bearing index, respectively. Fruits with less juice and mass were induced, respectively, by 'Riverside' citrandarin and 'Volkameriano' lemon. It can be concluded that the 'Mazoe' rootstock induces a higher fruit yield and 'Cravo' x 'Cleópatra' and 'Volkameriano', a greater survival. In addition, all rootstocks, except 'Riverside' and 'Volkameriano', induce fruit that meet the minimum quality requirements.},
keywords = {citrandarins, Citrus, coastal tablelands, Poncirus trifoliata, rainfed cultivation, scion/rootstocks combinations, tabuleiros costeiros},
pubstate = {published},
tppubtype = {article}
}
Abstract: The objective of this work was to determine the influence of eight rootstocks on the yield, fruit quality, and survival of 'Pêra CNPMF D6' sweet orange (Citrus sinensis) trees grown under rainfed conditions in a cohesive soil of the Brazilian Northeast. In 2014-2018, the yield, fruits, and survival of trees planted in 1997 were evaluated in a randomized complete block design. Yield was assessed using annual fruit production, yield efficiency, and the alternate bearing index. Tree survival was considered the percentage of plants that remained alive until 2018. Fruit quality was evaluated by physicochemical attributes. 'Cravo' confirmed its good yield performance. Despite the lower number of trees, the 'Mazoe' rough lemon rootstock induced the highest fruit yield. The 'Cravo' x 'Cleópatra' hybrid and 'Volkameriano' lemon favored the highest trees survival, whereas 'Indio' citrandarin and 'Cravo' lime resulted in intermediate levels. The 'San Diego' and 'Riverside' citrandarins induced shorter tree with a high yield efficiency and a higher alternate bearing index, respectively. Fruits with less juice and mass were induced, respectively, by 'Riverside' citrandarin and 'Volkameriano' lemon. It can be concluded that the 'Mazoe' rootstock induces a higher fruit yield and 'Cravo' x 'Cleópatra' and 'Volkameriano', a greater survival. In addition, all rootstocks, except 'Riverside' and 'Volkameriano', induce fruit that meet the minimum quality requirements.
Martins, Paula M. M.; Wood, Thomas K.; Souza, Alessandra A.
Persister Cells Form in the Plant Pathogen Xanthomonas citri subsp. citri under Different Stress Conditions Journal Article
In: Microorganisms 2021, Vol. 9, Page 384, vol. 9, iss. 2, pp. 384, 2021, ISSN: 2076-2607.
Abstract | Links | BibTeX | Tags: Citrus, Citrus canker, persistence, Phytopathogen
@article{Martins2021,
title = {Persister Cells Form in the Plant Pathogen Xanthomonas citri subsp. citri under Different Stress Conditions},
author = {Paula M. M. Martins and Thomas K. Wood and Alessandra A. Souza},
url = {https://www.mdpi.com/2076-2607/9/2/384/htm https://www.mdpi.com/2076-2607/9/2/384},
doi = {10.3390/MICROORGANISMS9020384},
issn = {2076-2607},
year = {2021},
date = {2021-01-01},
journal = {Microorganisms 2021, Vol. 9, Page 384},
volume = {9},
issue = {2},
pages = {384},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Citrus canker disease, caused by the bacterium Xanthomonas citri subsp. citri is a constant threat to citrus-producing areas. Since it has no cure, agricultural practices to restrain its dissemination are essential to reduce the economic damage. Hence, increased knowledge of the basic aspects of X. citri biology could lead to more efficient management practices that can eliminate dormant bacteria in the field. The dormant cells, also referred to as persisters, are phenotypic variants with lowered metabolism, which in turn leads to tolerance to antimicrobials and undermines existing control approaches. We show here that X. citri forms persisters, identifying triggers for this phenotype, including antibiotics, high temperature, and metals (copper and zinc), which increase persistence rates by 10–100 times. The antioxidant N-acetylcysteine reduced copper and zinc-induced persisters, but not those induced by tetracycline, indicating that oxidative stress may be an important inducer of X. citri persistence. In addition, we found that metabolism-independent drugs like cisplatin and mitomycin C are able to eliminate X. citri persistent cells, as well as copper, at high concentrations. Specific amino acids like proline and isoleucine interfered with the physiological balance of the dormancy in X. citri, stimulating or preventing persister resuscitation. Taken together, we discover chemicals that can induce, wake, and kill X. citri persister cells; these results provide insights that should be considered for more efficient integrated control management in the field.},
keywords = {Citrus, Citrus canker, persistence, Phytopathogen},
pubstate = {published},
tppubtype = {article}
}
Citrus canker disease, caused by the bacterium Xanthomonas citri subsp. citri is a constant threat to citrus-producing areas. Since it has no cure, agricultural practices to restrain its dissemination are essential to reduce the economic damage. Hence, increased knowledge of the basic aspects of X. citri biology could lead to more efficient management practices that can eliminate dormant bacteria in the field. The dormant cells, also referred to as persisters, are phenotypic variants with lowered metabolism, which in turn leads to tolerance to antimicrobials and undermines existing control approaches. We show here that X. citri forms persisters, identifying triggers for this phenotype, including antibiotics, high temperature, and metals (copper and zinc), which increase persistence rates by 10–100 times. The antioxidant N-acetylcysteine reduced copper and zinc-induced persisters, but not those induced by tetracycline, indicating that oxidative stress may be an important inducer of X. citri persistence. In addition, we found that metabolism-independent drugs like cisplatin and mitomycin C are able to eliminate X. citri persistent cells, as well as copper, at high concentrations. Specific amino acids like proline and isoleucine interfered with the physiological balance of the dormancy in X. citri, stimulating or preventing persister resuscitation. Taken together, we discover chemicals that can induce, wake, and kill X. citri persister cells; these results provide insights that should be considered for more efficient integrated control management in the field.
Mendes, Maria Inês Souza; Verde, Denise Santos Vila; Ramos, Andresa Priscila Souza; Gesteira, Abelmon Silva; Filho, Walter Santos Soares; Souza, Antônio Silva
In vitro conservation of citrus rootstocks using paclobutrazol and analysis of plant viability and genetic stability Journal Article
In: Scientia Horticulturae, vol. 286, pp. 110231, 2021, ISSN: 0304-4238.
Abstract | Links | BibTeX | Tags: Citrus, genetic resources, Growth retardant, Somaclonal variation, Tissue culture
@article{Mendes2021,
title = {In vitro conservation of citrus rootstocks using paclobutrazol and analysis of plant viability and genetic stability},
author = {Maria Inês Souza Mendes and Denise Santos Vila Verde and Andresa Priscila Souza Ramos and Abelmon Silva Gesteira and Walter Santos Soares Filho and Antônio Silva Souza},
doi = {10.1016/J.SCIENTA.2021.110231},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {286},
pages = {110231},
publisher = {Elsevier},
abstract = {The vulnerabilities to which citrus genetic resources are exposed under field conservation conditions make it important to ensure an in vitro backup. Thus, in this study, we aimed to establish a protocol for in vitro conservation of citrus rootstocks by analyzing the use of paclobutrazol to maintain a minimum growth and ensure the viability and genetic stability of conserved plants. In the first experiment, 1 cm-long apical microcuttings from plants of five rootstocks grown in vitro were placed in basal woody plant medium, supplemented with 0.2, 0.4, 0.6, 0.8, and 1.0 mg L−1 of paclobutrazol, and maintained in a conservation room at a temperature of 22 ± 1 °C, photoperiod of 12 h, and photon flux density of 20 μmol m−2 s−1 for 6 months. After this period, the plants from the medium selected for in vitro conservation of the rootstocks were acclimatized. For the second experiment, nucellar seedlings (confirmed by simple sequence repeat [SSR] markers) of 10 citrus rootstocks were multiplied and conserved in the previously defined medium. At the end of 12 months, the plants were subjected to three subculturing cycles to verify their viability. Molecular analyses were performed on plants from the third subculture using 21 SSR markers to detect somaclonal variants. Paclobutrazol was not efficient in maintaining reduced plant growth. Acclimatized plants grown in vitro exhibited 100% survival. SSR markers were efficient in characterizing the studied rootstocks. In general, the in vitro conservation conditions did not affect plant viability. The three subculturing cycles performed after conservation did not result in somaclonal variants. The conservation protocol described in this study can be used for the in vitro maintenance of the analyzed rootstocks and can be extended to other citrus genotypes.},
keywords = {Citrus, genetic resources, Growth retardant, Somaclonal variation, Tissue culture},
pubstate = {published},
tppubtype = {article}
}
The vulnerabilities to which citrus genetic resources are exposed under field conservation conditions make it important to ensure an in vitro backup. Thus, in this study, we aimed to establish a protocol for in vitro conservation of citrus rootstocks by analyzing the use of paclobutrazol to maintain a minimum growth and ensure the viability and genetic stability of conserved plants. In the first experiment, 1 cm-long apical microcuttings from plants of five rootstocks grown in vitro were placed in basal woody plant medium, supplemented with 0.2, 0.4, 0.6, 0.8, and 1.0 mg L−1 of paclobutrazol, and maintained in a conservation room at a temperature of 22 ± 1 °C, photoperiod of 12 h, and photon flux density of 20 μmol m−2 s−1 for 6 months. After this period, the plants from the medium selected for in vitro conservation of the rootstocks were acclimatized. For the second experiment, nucellar seedlings (confirmed by simple sequence repeat [SSR] markers) of 10 citrus rootstocks were multiplied and conserved in the previously defined medium. At the end of 12 months, the plants were subjected to three subculturing cycles to verify their viability. Molecular analyses were performed on plants from the third subculture using 21 SSR markers to detect somaclonal variants. Paclobutrazol was not efficient in maintaining reduced plant growth. Acclimatized plants grown in vitro exhibited 100% survival. SSR markers were efficient in characterizing the studied rootstocks. In general, the in vitro conservation conditions did not affect plant viability. The three subculturing cycles performed after conservation did not result in somaclonal variants. The conservation protocol described in this study can be used for the in vitro maintenance of the analyzed rootstocks and can be extended to other citrus genotypes.
Morelli, Marilia; Azevedo, Fernando Alves; Souza, Ana Julia Borim; Martinelli, Rodrigo; Conceição, Patrícia Marluci
Physiological Quality of Swingle Citrumelo Seed after Refrigerated Storage of Fruits and Seeds Journal Article
In: Agriculture 2021, Vol. 11, Page 1243, vol. 11, iss. 12, pp. 1243, 2021, ISSN: 2077-0472.
Abstract | Links | BibTeX | Tags: Citrus, propagation, rootstock
@article{Morelli2021,
title = {Physiological Quality of Swingle Citrumelo Seed after Refrigerated Storage of Fruits and Seeds},
author = {Marilia Morelli and Fernando Alves Azevedo and Ana Julia Borim Souza and Rodrigo Martinelli and Patrícia Marluci Conceição},
url = {https://www.mdpi.com/2077-0472/11/12/1243/htm https://www.mdpi.com/2077-0472/11/12/1243},
doi = {10.3390/AGRICULTURE11121243},
issn = {2077-0472},
year = {2021},
date = {2021-01-01},
journal = {Agriculture 2021, Vol. 11, Page 1243},
volume = {11},
issue = {12},
pages = {1243},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {For citrus, there is no definition of the ideal fruit harvesting point for seed extraction. Some studies have shown the positive effect of postharvest refrigerated storage of fruits to obtain quality seeds. This study aimed to evaluate the influence of the fruit maturation stage on the germination of Swingle citrumelo (CS) seeds after the refrigerated storage of fruits and seeds. CS fruits were harvested at 180 days after anthesis (DAA); 210 DAA and 240 DAA. Fruits and seeds were stored in cold chamber for 0, 15, 30, and 45 days. Physicochemical analyses were performed on fruits (colour, soluble solids, acidity and ratio) and seeds (water content, germination, and emergence). Germination of seeds extracted from fruits harvested at 180, 210, and 240 DAA, without refrigerated storage, showed a positive correlation with colour index. The refrigerated storage of fruits and seeds extracted from fruits harvested at 210 DAA, increased the seed germination rate. Peel colour (IC) was correlated positively with the germination of Swingle citrumelo seeds. The results of this study revealed that the absence of refrigerated storage, harvesting Swingle citrumelo fruits, with ripe characteristics (end of harvest), results in a high seed germination rate. On the other hand, refrigerated storage of fruits and seeds extracted from fruits harvested at mid-harvest increases the seed germination rate.},
keywords = {Citrus, propagation, rootstock},
pubstate = {published},
tppubtype = {article}
}
For citrus, there is no definition of the ideal fruit harvesting point for seed extraction. Some studies have shown the positive effect of postharvest refrigerated storage of fruits to obtain quality seeds. This study aimed to evaluate the influence of the fruit maturation stage on the germination of Swingle citrumelo (CS) seeds after the refrigerated storage of fruits and seeds. CS fruits were harvested at 180 days after anthesis (DAA); 210 DAA and 240 DAA. Fruits and seeds were stored in cold chamber for 0, 15, 30, and 45 days. Physicochemical analyses were performed on fruits (colour, soluble solids, acidity and ratio) and seeds (water content, germination, and emergence). Germination of seeds extracted from fruits harvested at 180, 210, and 240 DAA, without refrigerated storage, showed a positive correlation with colour index. The refrigerated storage of fruits and seeds extracted from fruits harvested at 210 DAA, increased the seed germination rate. Peel colour (IC) was correlated positively with the germination of Swingle citrumelo seeds. The results of this study revealed that the absence of refrigerated storage, harvesting Swingle citrumelo fruits, with ripe characteristics (end of harvest), results in a high seed germination rate. On the other hand, refrigerated storage of fruits and seeds extracted from fruits harvested at mid-harvest increases the seed germination rate.
Sampaio, Antonio Hélder Rodrigues; Silva, Roberval Oliveira; Brito, Ralph Bruno França; Filho, Walter Santos Soares; Gesteira, Abelmon Silva; Souza, Laércio Duarte; Filho, Mauricio Antonio Coelho
Sweet orange acclimatisation to water stress: a rootstock dependency Journal Article
In: Scientia Horticulturae, vol. 276, pp. 109727, 2021, ISSN: 0304-4238.
Abstract | Links | BibTeX | Tags: Citrus, Drought tolerance, plant and soil water potential, plant water relations
@article{Sampaio2021,
title = {Sweet orange acclimatisation to water stress: a rootstock dependency},
author = {Antonio Hélder Rodrigues Sampaio and Roberval Oliveira Silva and Ralph Bruno França Brito and Walter Santos Soares Filho and Abelmon Silva Gesteira and Laércio Duarte Souza and Mauricio Antonio Coelho Filho},
doi = {10.1016/J.SCIENTA.2020.109727},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {276},
pages = {109727},
publisher = {Elsevier},
abstract = {The diversification of rootstocks is of great importance for the survival of the citrus industry in order to reduce and mitigate risks caused by abiotic factors, primarily related to drought. The present study aimed to investigate the effects of ‘Pera’ sweet orange Citrus sinensis (L.) Osbeck interactions with eight rootstocks on soil water extraction, physiological responses and field productivity. Eight rootstocks combined with the ‘Pera’ sweet orange were evaluated in the field to study the effects on the following factors in response to soil water availability: matric potential (Ѱm) at depths of 0.25 m, 0.50 m and 0.90 m; physiological responses of the plants: leaf water potential (Ѱw), stomatic conductance (gs) and temperature (Lt); and plant productivity. Significant differences (p ≤ 0.05) were observed in the dry period for scion-rootstock interactions on the parameters Ѱm, Ѱw and gs. There were also significant differences for yield and productive efficiency (p ≤ 0.05). However, contrasting individuals concerning soil water extraction showed similar physiological responses in the driest periods. Different mechanisms of acclimatization to periods of drought stress that resulted in different soil water extractions and different physiological responses were not strongly related to plant productivity and were dependent on the characteristics of the deficit event. Water extraction from the shallow layer of soil is best correlated with plant water status Ѱw, it is not associated with the effective use of water in deeper soil layers for the scion-rootstock combinations studied, and explains the variation on productivities of genotypes. Scion-rootstock interactions that induce a smaller canopy volume and higher productive efficiency (kg fruit/volume canopy) and lower water use are more appropriated in high planting densities.},
keywords = {Citrus, Drought tolerance, plant and soil water potential, plant water relations},
pubstate = {published},
tppubtype = {article}
}
The diversification of rootstocks is of great importance for the survival of the citrus industry in order to reduce and mitigate risks caused by abiotic factors, primarily related to drought. The present study aimed to investigate the effects of ‘Pera’ sweet orange Citrus sinensis (L.) Osbeck interactions with eight rootstocks on soil water extraction, physiological responses and field productivity. Eight rootstocks combined with the ‘Pera’ sweet orange were evaluated in the field to study the effects on the following factors in response to soil water availability: matric potential (Ѱm) at depths of 0.25 m, 0.50 m and 0.90 m; physiological responses of the plants: leaf water potential (Ѱw), stomatic conductance (gs) and temperature (Lt); and plant productivity. Significant differences (p ≤ 0.05) were observed in the dry period for scion-rootstock interactions on the parameters Ѱm, Ѱw and gs. There were also significant differences for yield and productive efficiency (p ≤ 0.05). However, contrasting individuals concerning soil water extraction showed similar physiological responses in the driest periods. Different mechanisms of acclimatization to periods of drought stress that resulted in different soil water extractions and different physiological responses were not strongly related to plant productivity and were dependent on the characteristics of the deficit event. Water extraction from the shallow layer of soil is best correlated with plant water status Ѱw, it is not associated with the effective use of water in deeper soil layers for the scion-rootstock combinations studied, and explains the variation on productivities of genotypes. Scion-rootstock interactions that induce a smaller canopy volume and higher productive efficiency (kg fruit/volume canopy) and lower water use are more appropriated in high planting densities.
Santos, Ariana Silva; Andrade, Edson Mário; Sousa, Andressa Rodrigues Oliveira; Costa, Marcos Mota Carmo; Togawa, Roberto; Grynberg, Priscila; Pirovani, Carlos Priminho; Oliveira, Saulo Alves Silva; Filho, Walter Santos Soares; Filho, Mauricio Antônio Coelho; Gesteira, Abelmon Silva; Ferreira, Claudia Fortes
Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies Journal Article
In: Scientia Horticulturae, vol. 277, pp. 109838, 2021, ISSN: 0304-4238.
Abstract | Links | BibTeX | Tags: Citrus, drought, RNA-Seq, root, Sunki, Transcriptome
@article{nokey,
title = {Transcriptome profile of drought responsive candidate genes in varieties of citrus rootstocks with different tolerance strategies},
author = {Ariana Silva Santos and Edson Mário Andrade and Andressa Rodrigues Oliveira Sousa and Marcos Mota Carmo Costa and Roberto Togawa and Priscila Grynberg and Carlos Priminho Pirovani and Saulo Alves Silva Oliveira and Walter Santos Soares Filho and Mauricio Antônio Coelho Filho and Abelmon Silva Gesteira and Claudia Fortes Ferreira},
doi = {10.1016/J.SCIENTA.2020.109838},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {277},
pages = {109838},
publisher = {Elsevier},
abstract = {Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.},
keywords = {Citrus, drought, RNA-Seq, root, Sunki, Transcriptome},
pubstate = {published},
tppubtype = {article}
}
Sunki Maravilha' and 'Sunki Tropical' are citrus plants with different drought tolerance strategies. `Sunki Tropical' is considered as another rootstock alternative for use in drought prone areas, however, its genetic expression profiles regarding response to water deficit, are still unknown. In this study, the transcriptome of the ‘Sunki Maravilha’ and ‘Sunki Tropical’ rootstocks under control were analyzed by RNA-seq technique using the Illumina HiSeq-2500. A total of 109,114.164 reads were produced and mapped against the Citrus sinensis reference genome (version 1.1). 3.607 transcripts showed significant variation in expression between the water deficit and control treatments with 739 genes up-regulated and 2868 down-regulated during water deficit imposition. The differentially expressed genes (DEGs) identified are involved in more than 100 biological processes and molecular functions whereas most enriched for the function of oxidoreductase, protein phosporilation, transcriptional regulation, ligation processes and response to oxidative stress. DEGs related to the biosynthesis of ABA (Abscisic Acid), redox system, cell wall mechanism, root development, transcription factors (TFs) and kinase proteins (KPs), were also identified. This work lays the basic foundation for further investigation of the specific functions of genes in citrus rootstocks, Sunki Maravilha’ and Sunki Tropical’, with different strategies of survival under water deficit and also provides candidate genes for engineering or breeding of citrus plants aiming drought tolerance.
2020
Costa, Gabriela; Curtolo, Maiara; Magni, Thaís Cavichioli; Cristofani-Yaly, Mariângela
Response of citrus hybrids to Alternaria alternata inoculation Journal Article
In: Comunicata Scientiae, vol. 11, pp. e3358-e3358, 2020, ISSN: 2177-5133.
Abstract | Links | BibTeX | Tags: Alternaria brown spot, Citrus, Mandarin, Phytopathology, Tangor
@article{nokey,
title = {Response of citrus hybrids to Alternaria alternata inoculation},
author = {Gabriela Costa and Maiara Curtolo and Thaís Cavichioli Magni and Mariângela Cristofani-Yaly},
url = {https://www.comunicatascientiae.com.br/comunicata/article/view/3358},
doi = {10.14295/CS.V11I.3358},
issn = {2177-5133},
year = {2020},
date = {2020-01-01},
journal = {Comunicata Scientiae},
volume = {11},
pages = {e3358-e3358},
publisher = {Federal University ofPiauI},
abstract = {Citrus orchards have some limitations, such as the occurrence of phytosanitary problems. Alternaria brown spot (ABS) is caused by fungus Alternaria alternata, which affects several parts of the plant by producing a host-specific toxin, known as ACT. ABS is a limiting factor in orchards due to the susceptibility of most planted cultivars: ‘Murcott’ tangor and ‘Ponkan’ tangerine. The selection of varieties resistant/tolerant to the disease has economic importance. Therefore, the aim of this experiment was to evaluate the response to A. alternata inoculation in a population of ‘Murcott’ tangor vs ‘Pera’ sweet orange hybrids. Leaves of 2-3 centimeters in length of ‘Murcott’ tangor, ‘Pera’ sweet orange, ‘Ponkan’, ‘Dancy’, ‘Fremont’ tangerine and 198 hybrids were collected. For in vitro inoculation, monosporic A. alternata culture at concentration of 105 conidia mL-1 was used. Inoculated leaves were stored in humid chamber. After 24, 48 and 72 hours of inoculation, leaf lesions were evaluated following a diagrammatic scale. The results obtained showed that most hybrids from the crossing of ‘Murcott’ tangor vs ‘Pera’ sweet orange are susceptible to ABS. However, 44 are resistant and ten are tolerant. Among ABS-tolerant hybrids, some have phenotype similar to that of cultivated and commercialized hybrids.},
keywords = {Alternaria brown spot, Citrus, Mandarin, Phytopathology, Tangor},
pubstate = {published},
tppubtype = {article}
}
Citrus orchards have some limitations, such as the occurrence of phytosanitary problems. Alternaria brown spot (ABS) is caused by fungus Alternaria alternata, which affects several parts of the plant by producing a host-specific toxin, known as ACT. ABS is a limiting factor in orchards due to the susceptibility of most planted cultivars: ‘Murcott’ tangor and ‘Ponkan’ tangerine. The selection of varieties resistant/tolerant to the disease has economic importance. Therefore, the aim of this experiment was to evaluate the response to A. alternata inoculation in a population of ‘Murcott’ tangor vs ‘Pera’ sweet orange hybrids. Leaves of 2-3 centimeters in length of ‘Murcott’ tangor, ‘Pera’ sweet orange, ‘Ponkan’, ‘Dancy’, ‘Fremont’ tangerine and 198 hybrids were collected. For in vitro inoculation, monosporic A. alternata culture at concentration of 105 conidia mL-1 was used. Inoculated leaves were stored in humid chamber. After 24, 48 and 72 hours of inoculation, leaf lesions were evaluated following a diagrammatic scale. The results obtained showed that most hybrids from the crossing of ‘Murcott’ tangor vs ‘Pera’ sweet orange are susceptible to ABS. However, 44 are resistant and ten are tolerant. Among ABS-tolerant hybrids, some have phenotype similar to that of cultivated and commercialized hybrids.