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Check the list of papers from NScTI Citrus
Use the filters at the top of the list to select articles by year or by researcher. In the box for each article, use the links ("Summary", "Links", "BibTeX") to open additional information.
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2019
Silva, Caio Cesar Lima; Shimo, Hugo Massayoshi; Felício, Rafael; Mercaldi, Gustavo Fernando; Rocco, Silvana Aparecida; Benedetti, Celso Eduardo
Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance Journal Article
In: Scientific Reports 2019 9:1, vol. 9, iss. 1, pp. 1-12, 2019, ISSN: 2045-2322.
Abstract | Links | BibTeX | Tags: Biotic, Plant hormones
@article{nokey,
title = {Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance},
author = {Caio Cesar Lima Silva and Hugo Massayoshi Shimo and Rafael Felício and Gustavo Fernando Mercaldi and Silvana Aparecida Rocco and Celso Eduardo Benedetti},
url = {https://www.nature.com/articles/s41598-019-40552-3},
doi = {10.1038/s41598-019-40552-3},
issn = {2045-2322},
year = {2019},
date = {2019-01-01},
journal = {Scientific Reports 2019 9:1},
volume = {9},
issue = {1},
pages = {1-12},
publisher = {Nature Publishing Group},
abstract = {Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria.},
keywords = {Biotic, Plant hormones},
pubstate = {published},
tppubtype = {article}
}
Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria.
Silva, Caio Cesar Lima; Shimo, Hugo Massayoshi; Felício, Rafael; Mercaldi, Gustavo Fernando; Rocco, Silvana Aparecida; Benedetti, Celso Eduardo
Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance Journal Article
In: Scientific Reports 2019 9:1, vol. 9, iss. 1, pp. 1-12, 2019, ISSN: 2045-2322.
Abstract | Links | BibTeX | Tags: Biotic, Plant hormones
@article{nokey,
title = {Structure-function relationship of a citrus salicylate methylesterase and role of salicylic acid in citrus canker resistance},
author = {Caio Cesar Lima Silva and Hugo Massayoshi Shimo and Rafael Felício and Gustavo Fernando Mercaldi and Silvana Aparecida Rocco and Celso Eduardo Benedetti},
url = {https://www.nature.com/articles/s41598-019-40552-3},
doi = {10.1038/s41598-019-40552-3},
issn = {2045-2322},
year = {2019},
date = {2019-01-01},
journal = {Scientific Reports 2019 9:1},
volume = {9},
issue = {1},
pages = {1-12},
publisher = {Nature Publishing Group},
abstract = {Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria.},
keywords = {Biotic, Plant hormones},
pubstate = {published},
tppubtype = {article}
}
Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria.
2018
Arena, Gabriella D.; Ramos-González, Pedro L.; Rogerio, Luana A.; Ribeiro-Alves, Marcelo; Casteel, Clare L.; Freitas-Astúa, Juliana; Machado, Marcos A.
Making a better home: modulation of plant defensive response by brevipalpus mites Journal Article
In: Frontiers in Plant Science, vol. 9, pp. 1147, 2018, ISSN: 1664462X.
Abstract | Links | BibTeX | Tags: Cross-talk, Defense pathways, Jasmonic acid, Plant hormones, Plant–herbivore interaction, RNA-Seq, Salicylic acid, Tetranychus
@article{Arena2018,
title = {Making a better home: modulation of plant defensive response by brevipalpus mites},
author = {Gabriella D. Arena and Pedro L. Ramos-González and Luana A. Rogerio and Marcelo Ribeiro-Alves and Clare L. Casteel and Juliana Freitas-Astúa and Marcos A. Machado},
doi = {10.3389/FPLS.2018.01147/BIBTEX},
issn = {1664462X},
year = {2018},
date = {2018-01-01},
journal = {Frontiers in Plant Science},
volume = {9},
pages = {1147},
publisher = {Frontiers Media S.A.},
abstract = {False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growth-defense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the non-infested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JA-mediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway.},
keywords = {Cross-talk, Defense pathways, Jasmonic acid, Plant hormones, Plant–herbivore interaction, RNA-Seq, Salicylic acid, Tetranychus},
pubstate = {published},
tppubtype = {article}
}
False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growth-defense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the non-infested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JA-mediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway.