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2022
Merfa, Marcus Vinıcius; Fischer, Eduarda Regina; Silva, Mariana; Francisco, Carolina Sardinha; Coletta-Filho, Helvecio Della; Souza, Alessandra Alves
Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri Journal Article
In: Phytopathology, vol. 112, iss. 1, pp. 163-172, 2022, ISSN: 19437684.
Abstract | Links | BibTeX | Tags: Bacterial pathogens, Biotechnology, Disease control and pest management, genetics
@article{Merfa2022,
title = {Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri},
author = {Marcus Vinıcius Merfa and Eduarda Regina Fischer and Mariana Silva and Carolina Sardinha Francisco and Helvecio Della Coletta-Filho and Alessandra Alves Souza},
url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-06-21-0252-FI},
doi = {10.1094/PHYTO-06-21-0252-FI/ASSET/IMAGES/LARGE/PHYTO-06-21-0252-FIF6.JPEG},
issn = {19437684},
year = {2022},
date = {2022-01-01},
journal = {Phytopathology},
volume = {112},
issue = {1},
pages = {163-172},
publisher = {American Phytopathological Society},
abstract = {Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.},
keywords = {Bacterial pathogens, Biotechnology, Disease control and pest management, genetics},
pubstate = {published},
tppubtype = {article}
}
Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.
Vieira, Gabrielle; Khalil, Zeinab G.; Capon, Robert J.; Sette, Lara D.; Ferreira, Henrique; Sass, Daiane C.
Isolation and agricultural potential of penicillic acid against citrus canker Journal Article
In: Journal of Applied Microbiology, vol. 132, iss. 4, pp. 3081-3088, 2022, ISSN: 1365-2672.
Abstract | Links | BibTeX | Tags: agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases
@article{Vieira2022,
title = {Isolation and agricultural potential of penicillic acid against citrus canker},
author = {Gabrielle Vieira and Zeinab G. Khalil and Robert J. Capon and Lara D. Sette and Henrique Ferreira and Daiane C. Sass},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15413 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15413 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15413},
doi = {10.1111/JAM.15413},
issn = {1365-2672},
year = {2022},
date = {2022-01-01},
journal = {Journal of Applied Microbiology},
volume = {132},
issue = {4},
pages = {3081-3088},
publisher = {John Wiley & Sons, Ltd},
abstract = {Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.},
keywords = {agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases},
pubstate = {published},
tppubtype = {article}
}
Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.
2018
Savietto, Abigail; Polaquini, Carlos Roberto; Kopacz, Malgorzata; Scheffers, Dirk Jan; Marques, Beatriz Carvalho; Regasini, Luís Octavio; Ferreira, Henrique
Antibacterial activity of monoacetylated alkyl gallates against Xanthomonas citri subsp. citri Journal Article
In: Archives of Microbiology 2018 200:6, vol. 200, iss. 6, pp. 929-937, 2018, ISSN: 1432-072X.
Abstract | Links | BibTeX | Tags: Biochemistry, Biotechnology, Cell Biology, Ecology, general, Microbial Ecology, Microbiology
@article{Savietto2018,
title = {Antibacterial activity of monoacetylated alkyl gallates against Xanthomonas citri subsp. citri},
author = {Abigail Savietto and Carlos Roberto Polaquini and Malgorzata Kopacz and Dirk Jan Scheffers and Beatriz Carvalho Marques and Luís Octavio Regasini and Henrique Ferreira},
url = {https://link.springer.com/article/10.1007/s00203-018-1502-6},
doi = {10.1007/S00203-018-1502-6},
issn = {1432-072X},
year = {2018},
date = {2018-01-01},
journal = {Archives of Microbiology 2018 200:6},
volume = {200},
issue = {6},
pages = {929-937},
publisher = {Springer},
abstract = {Asiatic citrus canker (ACC) is an incurable disease of citrus plants caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (X. citri). It affects all the commercially important citrus varieties in the major orange producing areas around the world. Control of the pathogen requires recurrent sprays of copper formulations that accumulate in soil and water reservoirs. Here, we describe the improvement of the alkyl gallates, which are potent anti-X. citri compounds, intended to be used as alternatives to copper in the control of ACC. Acetylation of alkyl gallates increased their lipophilicity, which resulted in potentiation of the antibacterial activity. X. citri exposed to the acetylated compounds exhibited increased cell length that is consistent with the disruption of the cell division apparatus. Finally, we show that inhibition of cell division is an indirect effect that seemed to be caused by membrane permeabilization, which is apparently the primary target of the acetylated alkyl gallates.},
keywords = {Biochemistry, Biotechnology, Cell Biology, Ecology, general, Microbial Ecology, Microbiology},
pubstate = {published},
tppubtype = {article}
}
Asiatic citrus canker (ACC) is an incurable disease of citrus plants caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (X. citri). It affects all the commercially important citrus varieties in the major orange producing areas around the world. Control of the pathogen requires recurrent sprays of copper formulations that accumulate in soil and water reservoirs. Here, we describe the improvement of the alkyl gallates, which are potent anti-X. citri compounds, intended to be used as alternatives to copper in the control of ACC. Acetylation of alkyl gallates increased their lipophilicity, which resulted in potentiation of the antibacterial activity. X. citri exposed to the acetylated compounds exhibited increased cell length that is consistent with the disruption of the cell division apparatus. Finally, we show that inhibition of cell division is an indirect effect that seemed to be caused by membrane permeabilization, which is apparently the primary target of the acetylated alkyl gallates.