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2022
Merfa, Marcus Vinıcius; Fischer, Eduarda Regina; Silva, Mariana; Francisco, Carolina Sardinha; Coletta-Filho, Helvecio Della; Souza, Alessandra Alves
Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri Journal Article
In: Phytopathology, vol. 112, iss. 1, pp. 163-172, 2022, ISSN: 19437684.
Abstract | Links | BibTeX | Tags: Bacterial pathogens, Biotechnology, Disease control and pest management, genetics
@article{Merfa2022,
title = {Probing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of ‘Candidatus Liberibacter asiaticus’ by Diaphorina citri},
author = {Marcus Vinıcius Merfa and Eduarda Regina Fischer and Mariana Silva and Carolina Sardinha Francisco and Helvecio Della Coletta-Filho and Alessandra Alves Souza},
url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-06-21-0252-FI},
doi = {10.1094/PHYTO-06-21-0252-FI/ASSET/IMAGES/LARGE/PHYTO-06-21-0252-FIF6.JPEG},
issn = {19437684},
year = {2022},
date = {2022-01-01},
journal = {Phytopathology},
volume = {112},
issue = {1},
pages = {163-172},
publisher = {American Phytopathological Society},
abstract = {Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.},
keywords = {Bacterial pathogens, Biotechnology, Disease control and pest management, genetics},
pubstate = {published},
tppubtype = {article}
}
Huanglongbing (HLB) is currently the most devastating disease of citrus worldwide. Both bacteria ‘Candidatus Liberibacter asiaticus’ (CLas) and ‘Candidatus Liberibacter americanus’ (CLam) are associated with HLB in Brazil but with a strong prevalence of CLas over CLam. Conventionally, HLB management focuses on controlling the insect vector population (Diaphorina citri; also known as Asian citrus psyllid [ACP]) by spraying insecticides, an approach demonstrated to be mostly ineffective. Thus, development of novel, more efficient HLB control strategies is required. The multifunctional bacterial outer membrane protein OmpA is involved in several molecular processes between bacteria and their hosts and has been suggested as a target for bacterial control. Curiously, OmpA is absent in CLam in comparison with CLas, suggesting a possible role in host interaction. Therefore, in the current study, we have treated ACPs with different OmpA-derived peptides, aiming to evaluate acquisition of CLas by the insect vector. Treatment of psyllids with 5 µM of Pep1, Pep3, Pep5, and Pep6 in artificial diet significantly reduced the acquisition of CLas, whereas increasing the concentration of Pep5 and Pep6 to 50 µM abolished this process. In addition, in planta treatment with 50 µM of Pep6 also significantly decreased the acquisition of CLas, and sweet orange plants stably absorbed and maintained this peptide for as long as 3 months post the final application. Together, our results demonstrate the promising use of OmpA-derived peptides as a novel biotechnological tool to control CLas.
2020
Perino, Elvio Henrique Benatto; Glienke, Chirlei; Silva, Alan Oliveira; Deising, Holger B.
In: Phytopathology, vol. 110, iss. 9, pp. 1530-1540, 2020, ISSN: 19437684.
Abstract | Links | BibTeX | Tags: Acetohydroxamic acid, Allantoicase, Ammonium, Appressorial penetration, Biochemistry and cell biology, Disease control and pest management, Mycology, Nitrogen limitation, Novel plant health strategy, Phytophthora, Powdery mildew, Rust, Urease
@article{Perino2020,
title = {Molecular characterization of the purine degradation pathway genes ALA1 and URE1 of the maize anthracnose fungus colletotrichum graminicola identified urease as a novel target for plant disease control},
author = {Elvio Henrique Benatto Perino and Chirlei Glienke and Alan Oliveira Silva and Holger B. Deising},
url = {https://apsjournals.apsnet.org/doi/10.1094/PHYTO-04-20-0114-R},
doi = {10.1094/PHYTO-04-20-0114-R/ASSET/IMAGES/LARGE/PHYTO-04-20-0114-RF6.JPEG},
issn = {19437684},
year = {2020},
date = {2020-01-01},
journal = {Phytopathology},
volume = {110},
issue = {9},
pages = {1530-1540},
publisher = {American Phytopathological Society},
abstract = {Fungal pathogenicity is governed by environmental factors, with nitrogen playing a key role in triggering pathogenic development. Spores germinating on the plant cuticle are exposed to a nitrogen-free environment, and reprograming of nitrogen metabolism is required for bridging the time needed to gain access to the nitrogen sources of the host. Although degradation of endogenous purine bases efficiently generates ammonium and may allow the fungus to bridge the preinvasion nitrogen gap, the roles of the purine degradation pathway and of the key genes encoding allantoicase and urease are largely unknown in plant pathogenic fungi. To investigate the roles of the allantoicase and urease genes ALA1 and URE1 of the maize anthracnose fungus Colletotrichum graminicola in pathogenic development, we generated ALA1:eGFP and URE1:eGFP fusion strains as well as allantoicase- and urease-deficient mutants. Virulence assays, live cell, and differential interference contrast imaging, chemical complementation and employment of a urease inhibitor showed that the purine degradation genes ALA1 and URE1 are required for bridging nitrogen deficiency at early phases of the infection process and for full virulence. Application of the urease inhibitor acetohydroxamic acid did not only protect maize from C. graminicola infection, but also interfered with the infection process of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici, the maize and broad bean rusts Puccinia sorghi and Uromyces viciae-fabae, and the potato late blight pathogen Phytophthora infestans. Our data strongly suggest that inhibition of the purine degradation pathway might represent a novel approach to control plant pathogenic fungi and oomycetes.},
keywords = {Acetohydroxamic acid, Allantoicase, Ammonium, Appressorial penetration, Biochemistry and cell biology, Disease control and pest management, Mycology, Nitrogen limitation, Novel plant health strategy, Phytophthora, Powdery mildew, Rust, Urease},
pubstate = {published},
tppubtype = {article}
}
Fungal pathogenicity is governed by environmental factors, with nitrogen playing a key role in triggering pathogenic development. Spores germinating on the plant cuticle are exposed to a nitrogen-free environment, and reprograming of nitrogen metabolism is required for bridging the time needed to gain access to the nitrogen sources of the host. Although degradation of endogenous purine bases efficiently generates ammonium and may allow the fungus to bridge the preinvasion nitrogen gap, the roles of the purine degradation pathway and of the key genes encoding allantoicase and urease are largely unknown in plant pathogenic fungi. To investigate the roles of the allantoicase and urease genes ALA1 and URE1 of the maize anthracnose fungus Colletotrichum graminicola in pathogenic development, we generated ALA1:eGFP and URE1:eGFP fusion strains as well as allantoicase- and urease-deficient mutants. Virulence assays, live cell, and differential interference contrast imaging, chemical complementation and employment of a urease inhibitor showed that the purine degradation genes ALA1 and URE1 are required for bridging nitrogen deficiency at early phases of the infection process and for full virulence. Application of the urease inhibitor acetohydroxamic acid did not only protect maize from C. graminicola infection, but also interfered with the infection process of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici, the maize and broad bean rusts Puccinia sorghi and Uromyces viciae-fabae, and the potato late blight pathogen Phytophthora infestans. Our data strongly suggest that inhibition of the purine degradation pathway might represent a novel approach to control plant pathogenic fungi and oomycetes.