Publications

@article{Moreira2018,

title = {Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages in Bixa orellana L.},

author = {Viviane S. Moreira and Virgínia L. F. Soares and Raner J. S. Silva and Aurizangela O. Sousa and Wagner C. Otoni and Marcio G. C. Costa},

url = {https://link.springer.com/article/10.1007/s12298-018-0528-1},

doi = {10.1007/S12298-018-0528-1},

issn = {0974-0430},

year  = {2018},

date = {2018-01-01},

journal = {Physiology and Molecular Biology of Plants 2018 24:3},

volume = {24},

issue = {3},

pages = {369-378},

publisher = {Springer},

abstract = {Bixa orellana L., popularly known as annatto, produces several secondary metabolites of pharmaceutical and industrial interest, including bixin, whose molecular basis of biosynthesis remain to be determined. Gene expression analysis by quantitative real-time PCR (qPCR) is an important tool to advance such knowledge. However, correct interpretation of qPCR data requires the use of suitable reference genes in order to reduce experimental variations. In the present study, we have selected four different candidates for reference genes in B. orellana, coding for 40S ribosomal protein S9 (RPS9), histone H4 (H4), 60S ribosomal protein L38 (RPL38) and 18S ribosomal RNA (18SrRNA). Their expression stabilities in different tissues (e.g. flower buds, flowers, leaves and seeds at different developmental stages) were analyzed using five statistical tools (NormFinder, geNorm, BestKeeper, ΔCt method and RefFinder). The results indicated that RPL38 is the most stable gene in different tissues and stages of seed development and 18SrRNA is the most unstable among the analyzed genes. In order to validate the candidate reference genes, we have analyzed the relative expression of a target gene coding for carotenoid cleavage dioxygenase 1 (CCD1) using the stable RPL38 and the least stable gene, 18SrRNA, for normalization of the qPCR data. The results demonstrated significant differences in the interpretation of the CCD1 gene expression data, depending on the reference gene used, reinforcing the importance of the correct selection of reference genes for normalization.},

keywords = {Biological and Medical Physics, Biophysics, Cell Biology, Plant physiology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{Savietto2018,

title = {Antibacterial activity of monoacetylated alkyl gallates against Xanthomonas citri subsp. citri},

author = {Abigail Savietto and Carlos Roberto Polaquini and Malgorzata Kopacz and Dirk Jan Scheffers and Beatriz Carvalho Marques and Luís Octavio Regasini and Henrique Ferreira},

url = {https://link.springer.com/article/10.1007/s00203-018-1502-6},

doi = {10.1007/S00203-018-1502-6},

issn = {1432-072X},

year  = {2018},

date = {2018-01-01},

journal = {Archives of Microbiology 2018 200:6},

volume = {200},

issue = {6},

pages = {929-937},

publisher = {Springer},

abstract = {Asiatic citrus canker (ACC) is an incurable disease of citrus plants caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (X. citri). It affects all the commercially important citrus varieties in the major orange producing areas around the world. Control of the pathogen requires recurrent sprays of copper formulations that accumulate in soil and water reservoirs. Here, we describe the improvement of the alkyl gallates, which are potent anti-X. citri compounds, intended to be used as alternatives to copper in the control of ACC. Acetylation of alkyl gallates increased their lipophilicity, which resulted in potentiation of the antibacterial activity. X. citri exposed to the acetylated compounds exhibited increased cell length that is consistent with the disruption of the cell division apparatus. Finally, we show that inhibition of cell division is an indirect effect that seemed to be caused by membrane permeabilization, which is apparently the primary target of the acetylated alkyl gallates.},

keywords = {Biochemistry, Biotechnology, Cell Biology, Ecology, general, Microbial Ecology, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{Cardoso2017,

title = {Somatic embryogenesis of a seedless sweet orange (Citrus sinensis (L.) Osbeck)},

author = {Jean C. Cardoso and Maisa Curtolo and Rodrigo R. Latado and Adriana P. Martinelli},

url = {https://link.springer.com/article/10.1007/s11627-017-9866-x},

doi = {10.1007/S11627-017-9866-X},

issn = {1475-2689},

year  = {2017},

date = {2017-01-01},

journal = {In Vitro Cellular & Developmental Biology - Plant 2017 53:6},

volume = {53},

issue = {6},

pages = {619-623},

publisher = {Springer},

abstract = {Somatic embryogenesis is an important in vitro technique used to obtain Citrus sinensis (L.) Osbeck (sweet orange) plantlets for conservation, sanitation, propagation, and breeding. The induction of somatic embryogenesis from adult tissues of sweet orange could be an alternative to in vitro organogenesis from epicotyl segments, especially in seedless cultivars, where the latter is not feasible. The aim of this study was to obtain plantlets from ovary-derived somatic embryos of sweet orange cv. ‘Washington Navel’, an important seedless cultivar for citrus fresh fruit production. The explants used were pistils from flower buds, pre-anthesis, from 20-y-old plants cultivated in the field. Forty plantlets from 47 somatic embryos were obtained, in vitro-grafted, and acclimatized in greenhouse conditions. Ploidy evaluation through flow cytometric analysis, as well as the results of target region amplification polymorphism (TRAP) molecular markers confirmed the somatic origin of embryos as genetically similar to donor plants. This technique could be used for obtaining embryogenic cell suspension cultures or regenerated plants from mature tissues other than seed-derived tissues, especially for seedless genotypes.},

keywords = {Cell Biology, Developmental Biology, Plant Breeding/Biotechnology, Plant Genetics and Genomics, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}