Publications

@article{Vieira2022,

title = {Isolation and agricultural potential of penicillic acid against citrus canker},

author = {Gabrielle Vieira and Zeinab G. Khalil and Robert J. Capon and Lara D. Sette and Henrique Ferreira and Daiane C. Sass},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15413 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15413 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15413},

doi = {10.1111/JAM.15413},

issn = {1365-2672},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Applied Microbiology},

volume = {132},

issue = {4},

pages = {3081-3088},

publisher = {John Wiley & Sons, Ltd},

abstract = {Aims: The control of Xanthomonas citri subsp. citri (X. citri), causal agent of citrus canker, relies heavily on integrated agricultural practices involving the use of copper-based chemicals. Considering the need for alternatives to control this disease and the potential of fungi from extreme environments as producers of bioactive metabolites, we isolated and identified a bioactive compound from Penicillium sp. CRM 1540 isolated from Antarctica marine sediment. Methods and Results: The potential of compound as an antibacterial agent against X. citri was assessed through in vitro and greenhouse experiments. Molecular taxonomy indicates that this fungus is a possible new species of Penicillium. Results revealed 90% bacterial inhibition in vitro at 25 µg ml–1 and a decrease in 75.37% of citrus canker symptoms emergency in vivo in treated leaves of Citrus sinensis (L.) Osbeck considering the number of lesions per cm2 (p < 0.05) in comparison with the control. The structure of the active agent was identified as penicillic acid based on a detailed spectroscopic analysis. Conclusion: Penicillic acid can be an alternative against citrus canker. Significance and Impact of Study: Research into extremophile micro-organisms can identify molecules with biotechnological potential and alternatives to current agricultural practices.},

keywords = {agriculture, Antimicrobials, bioproducts, Biotechnology, plant diseases},

pubstate = {published},

tppubtype = {article}

}

@article{Brito2018,

title = {Emergence and morphophysiology of Sunki mandarin and other citrus genotypes seedlings under saline stress},

author = {Marcos E. B. Brito and Lauriane A. A. Soares and Walter S. Soares Filho and Pedro D. Fernandes and Elaine C. B. Silva and Francisco V. S. Sá and Luderlândio A. Silva},

url = {https://revistas.inia.es/index.php/sjar/article/view/9400/3932 https://revistas.inia.es/index.php/sjar/article/view/9400},

doi = {10.5424/SJAR/2018161-9400},

issn = {2171-9292},

year  = {2018},

date = {2018-01-01},

journal = {Spanish Journal of Agricultural Research},

volume = {16},

issue = {1},

pages = {e0801-e0801},

publisher = {Ministerio de Agricultura Pesca y Alimentacion},

abstract = {The scarcity of good quality water is a limiting factor for irrigated agriculture, especially in arid and semiarid regions, where water sources generally contain high concentrations of ions. In such conditions, it is essential to cultivate genotypes with economic potential and tolerance to salinity. Considering the importance of citrus and the need to identify genetic materials that adapt to saline stress, this study evaluates the salinity tolerance of 10 genotypes with rootstock potential. For the irrigation water, five levels of electrical conductivity (ECw: 0.8, 1.6, 2.4, 3.2 and 4.0 dS/m at 25 °C) were used in a randomized block design with three replications, and seed germination and growth variables, as well as physiological plant parameters, were evaluated. The germination rate of the hybrid TSKC × CTARG – 019 was the best under salt conditions, as it was the genotype with the highest tolerance to salinity in the germination stage. Salinity reduced the growth of the citrus genotypes, with the possibility of using water with EC of up to 1.6 dS/m in the rootstock formation stage. Salt stress affected the photosynthetic rate of the genotypes ‘San Diego’ citrandarin and TSKC × CTSW – 018 by reducing the stomatal conductance, restricting the diffusion of CO2 into the substomatal camera. The genotypes TSKC × CTARG – 019, TSKC × CTTR – 012 and TSKC × TRBK – 007 have the greatest potential for growth and photosynthetic apparatus efficiency when subjected to salinity.},

keywords = {agriculture, breeding, Citrus spp., diseases, economics, fertilization, genetic resources, genetics, growth, irrigation, livestock, pests, photosynthesis, physiology, reproduction, rootstock, Salinity, soils, water manage, weeds},

pubstate = {published},

tppubtype = {article}

}

@article{Martins2017b,

title = {Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions},

author = {Cristina P. S. Martins and Diana M. Neves and Luciana C. Cidade and Amanda F. S. Mendes and Delmira C. Silva and Alex Alan F. Almeida and Mauricio A. Coelho-Filho and Abelmon S. Gesteira and Walter S. Soares-Filho and Marcio G. C. Costa},

url = {https://link.springer.com/article/10.1007/s00425-017-2653-4},

doi = {10.1007/S00425-017-2653-4},

issn = {1432-2048},

year  = {2017},

date = {2017-01-01},

journal = {Planta 2017 245:5},

volume = {245},

issue = {5},

pages = {951-963},

publisher = {Springer},

abstract = {Overexpression of the citrus CsTIP2;1 improves plant growth and tolerance to salt and drought stresses by enhancing cell expansion, H 2 O 2 detoxification and stomatal conductance. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing disease, suggesting its involvement in the regulation of the flow of water and nutrients required during both normal growth and stress conditions. Here, we show that the overexpression of CsTIP2;1 in transgenic tobacco increases plant growth under optimal and water- and salt-stress conditions and also significantly improves the leaf water and oxidative status, photosynthetic capacity, transpiration rate and water use efficiency of plants subjected to a progressive soil drying. These results correlated with the enhanced mesophyll cell expansion, midrib aquiferous parenchyma abundance, H2O2 detoxification and stomatal conductance observed in the transgenic plants. Taken together, our results indicate that CsTIP2;1 plays an active role in regulating the water and oxidative status required for plant growth and adaptation to stressful environmental conditions and may be potentially useful for engineering stress tolerance in citrus and other crop plants.},

keywords = {agriculture, Ecology, Forestry, Orange, photosynthesis, Plant Sciences, Reactive oxygen species},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},

author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},

doi = {10.1007/S13313-017-0476-1},

issn = {1448-6032},

year  = {2017},

date = {2017-01-01},

journal = {Australasian Plant Pathology 2017 46:2},

volume = {46},

issue = {2},

pages = {191-203},

publisher = {Springer},

abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},

keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},

author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},

doi = {10.1007/S13313-017-0476-1},

issn = {1448-6032},

year  = {2017},

date = {2017-01-01},

journal = {Australasian Plant Pathology 2017 46:2},

volume = {46},

issue = {2},

pages = {191-203},

publisher = {Springer},

abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},

keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{Martins2017,

title = {Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions},

author = {Cristina P. S. Martins and Diana M. Neves and Luciana C. Cidade and Amanda F. S. Mendes and Delmira C. Silva and Alex Alan F. Almeida and Mauricio A. Coelho-Filho and Abelmon S. Gesteira and Walter S. Soares-Filho and Marcio G. C. Costa},

url = {https://link.springer.com/article/10.1007/s00425-017-2653-4},

doi = {10.1007/S00425-017-2653-4},

issn = {1432-2048},

year  = {2017},

date = {2017-01-01},

journal = {Planta 2017 245:5},

volume = {245},

issue = {5},

pages = {951-963},

publisher = {Springer},

abstract = {Overexpression of the citrus CsTIP2;1 improves plant growth and tolerance to salt and drought stresses by enhancing cell expansion, H 2 O 2 detoxification and stomatal conductance. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing disease, suggesting its involvement in the regulation of the flow of water and nutrients required during both normal growth and stress conditions. Here, we show that the overexpression of CsTIP2;1 in transgenic tobacco increases plant growth under optimal and water- and salt-stress conditions and also significantly improves the leaf water and oxidative status, photosynthetic capacity, transpiration rate and water use efficiency of plants subjected to a progressive soil drying. These results correlated with the enhanced mesophyll cell expansion, midrib aquiferous parenchyma abundance, H2O2 detoxification and stomatal conductance observed in the transgenic plants. Taken together, our results indicate that CsTIP2;1 plays an active role in regulating the water and oxidative status required for plant growth and adaptation to stressful environmental conditions and may be potentially useful for engineering stress tolerance in citrus and other crop plants.},

keywords = {agriculture, Ecology, Forestry, Orange, photosynthesis, Plant Sciences, Reactive oxygen species},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},

author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},

doi = {10.1007/S13313-017-0476-1},

issn = {1448-6032},

year  = {2017},

date = {2017-01-01},

journal = {Australasian Plant Pathology 2017 46:2},

volume = {46},

issue = {2},

pages = {191-203},

publisher = {Springer},

abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},

keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}