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2020
Dorta, Sílvia Oliveira; Balbinotte, Juliana; Monnerat, Rose; Lopes, João Roberto Spotti; Cunha, Tatiane; Zanardi, Odimar Zanuzo; Miranda, Marcelo Pedreira; Machado, Marcos Antonio; Freitas-Astúa, Juliana
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs Journal Article
In: Insect Science, vol. 27, iss. 3, pp. 519-530, 2020, ISSN: 1744-7917.
Abstract | Links | BibTeX | Tags: Asian citrus psyllid, Citrus sinensis, Cry toxins, endophytic Bt, huanglongbing, young shoots
@article{Dorta2020,
title = {Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs},
author = {Sílvia Oliveira Dorta and Juliana Balbinotte and Rose Monnerat and João Roberto Spotti Lopes and Tatiane Cunha and Odimar Zanuzo Zanardi and Marcelo Pedreira Miranda and Marcos Antonio Machado and Juliana Freitas-Astúa},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/1744-7917.12654 https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-7917.12654 https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12654},
doi = {10.1111/1744-7917.12654},
issn = {1744-7917},
year = {2020},
date = {2020-01-01},
journal = {Insect Science},
volume = {27},
issue = {3},
pages = {519-530},
publisher = {John Wiley & Sons, Ltd},
abstract = {Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of “Candidatus Liberibacter spp.,” associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%–77% and 66%–90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.},
keywords = {Asian citrus psyllid, Citrus sinensis, Cry toxins, endophytic Bt, huanglongbing, young shoots},
pubstate = {published},
tppubtype = {article}
}
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of “Candidatus Liberibacter spp.,” associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%–77% and 66%–90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.