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2020
Carmo-Sousa, Michele; Cortés, Mayerli Tatiana Borbón; Lopes, João Roberto Spotti
Understanding psyllid transmission of Candidatus Liberibacter as a basis for managing huanglongbing Journal Article
In: Tropical Plant Pathology, vol. 45, iss. 6, pp. 572-585, 2020, ISSN: 19832052.
Abstract | Links | BibTeX | Tags: Asian citrus psyllid, Disease management, HLB, Vector-pathogen interactions
@article{nokey,
title = {Understanding psyllid transmission of Candidatus Liberibacter as a basis for managing huanglongbing},
author = {Michele Carmo-Sousa and Mayerli Tatiana Borbón Cortés and João Roberto Spotti Lopes},
url = {https://link.springer.com/article/10.1007/s40858-020-00386-1},
doi = {10.1007/S40858-020-00386-1/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {572-585},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Citrus huanglongbing (HLB) represents a major threat to citriculture due to its rapid spread, difficulty of control, severe yield losses, and substantial increases in production costs in HLB-affected orchards. Successful management depends on detailed knowledge of the epidemiology of this disease, which is associated with phloem-limited bacteria of the genus Candidatus Liberibacter that are transmitted by phloem sap–feeding insects known as psyllids (Hemiptera: Psylloidea). Candidatus L. asiaticus (CLas) and the Asian citrus psyllid Diaphorina citri Kuwayama are the major etiological agent and vector, respectively, in the Americas and Asia. This review provides up-to-date information on the relationships of psyllid vectors with Ca. Liberibacter spp. associated with HLB, with emphasis on D. citri and CLas. Knowledge of the transmission mechanisms of these bacteria, as well as aspects of the biology, ecology, and behavior of the vector in relation to the environment, is essential to gain understanding of the HLB epidemics. Such knowledge provides a basis for developing innovative control methods and establishing sustainable management strategies for HLB.},
keywords = {Asian citrus psyllid, Disease management, HLB, Vector-pathogen interactions},
pubstate = {published},
tppubtype = {article}
}
Citrus huanglongbing (HLB) represents a major threat to citriculture due to its rapid spread, difficulty of control, severe yield losses, and substantial increases in production costs in HLB-affected orchards. Successful management depends on detailed knowledge of the epidemiology of this disease, which is associated with phloem-limited bacteria of the genus Candidatus Liberibacter that are transmitted by phloem sap–feeding insects known as psyllids (Hemiptera: Psylloidea). Candidatus L. asiaticus (CLas) and the Asian citrus psyllid Diaphorina citri Kuwayama are the major etiological agent and vector, respectively, in the Americas and Asia. This review provides up-to-date information on the relationships of psyllid vectors with Ca. Liberibacter spp. associated with HLB, with emphasis on D. citri and CLas. Knowledge of the transmission mechanisms of these bacteria, as well as aspects of the biology, ecology, and behavior of the vector in relation to the environment, is essential to gain understanding of the HLB epidemics. Such knowledge provides a basis for developing innovative control methods and establishing sustainable management strategies for HLB.
Carmo-Sousa, Michele; Cortés, Mayerli Tatiana Borbón; Lopes, João Roberto Spotti
Understanding psyllid transmission of Candidatus Liberibacter as a basis for managing huanglongbing Journal Article
In: Tropical Plant Pathology, vol. 45, iss. 6, pp. 572-585, 2020, ISSN: 19832052.
Abstract | Links | BibTeX | Tags: Asian citrus psyllid, Disease management, HLB, Vector-pathogen interactions
@article{nokey,
title = {Understanding psyllid transmission of Candidatus Liberibacter as a basis for managing huanglongbing},
author = {Michele Carmo-Sousa and Mayerli Tatiana Borbón Cortés and João Roberto Spotti Lopes},
url = {https://link.springer.com/article/10.1007/s40858-020-00386-1},
doi = {10.1007/S40858-020-00386-1/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {572-585},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Citrus huanglongbing (HLB) represents a major threat to citriculture due to its rapid spread, difficulty of control, severe yield losses, and substantial increases in production costs in HLB-affected orchards. Successful management depends on detailed knowledge of the epidemiology of this disease, which is associated with phloem-limited bacteria of the genus Candidatus Liberibacter that are transmitted by phloem sap–feeding insects known as psyllids (Hemiptera: Psylloidea). Candidatus L. asiaticus (CLas) and the Asian citrus psyllid Diaphorina citri Kuwayama are the major etiological agent and vector, respectively, in the Americas and Asia. This review provides up-to-date information on the relationships of psyllid vectors with Ca. Liberibacter spp. associated with HLB, with emphasis on D. citri and CLas. Knowledge of the transmission mechanisms of these bacteria, as well as aspects of the biology, ecology, and behavior of the vector in relation to the environment, is essential to gain understanding of the HLB epidemics. Such knowledge provides a basis for developing innovative control methods and establishing sustainable management strategies for HLB.},
keywords = {Asian citrus psyllid, Disease management, HLB, Vector-pathogen interactions},
pubstate = {published},
tppubtype = {article}
}
Citrus huanglongbing (HLB) represents a major threat to citriculture due to its rapid spread, difficulty of control, severe yield losses, and substantial increases in production costs in HLB-affected orchards. Successful management depends on detailed knowledge of the epidemiology of this disease, which is associated with phloem-limited bacteria of the genus Candidatus Liberibacter that are transmitted by phloem sap–feeding insects known as psyllids (Hemiptera: Psylloidea). Candidatus L. asiaticus (CLas) and the Asian citrus psyllid Diaphorina citri Kuwayama are the major etiological agent and vector, respectively, in the Americas and Asia. This review provides up-to-date information on the relationships of psyllid vectors with Ca. Liberibacter spp. associated with HLB, with emphasis on D. citri and CLas. Knowledge of the transmission mechanisms of these bacteria, as well as aspects of the biology, ecology, and behavior of the vector in relation to the environment, is essential to gain understanding of the HLB epidemics. Such knowledge provides a basis for developing innovative control methods and establishing sustainable management strategies for HLB.
Dorta, Sílvia Oliveira; Balbinotte, Juliana; Monnerat, Rose; Lopes, João Roberto Spotti; Cunha, Tatiane; Zanardi, Odimar Zanuzo; Miranda, Marcelo Pedreira; Machado, Marcos Antonio; Freitas-Astúa, Juliana
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs Journal Article
In: Insect Science, vol. 27, iss. 3, pp. 519-530, 2020, ISSN: 1744-7917.
Abstract | Links | BibTeX | Tags: Asian citrus psyllid, Citrus sinensis, Cry toxins, endophytic Bt, huanglongbing, young shoots
@article{Dorta2020,
title = {Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs},
author = {Sílvia Oliveira Dorta and Juliana Balbinotte and Rose Monnerat and João Roberto Spotti Lopes and Tatiane Cunha and Odimar Zanuzo Zanardi and Marcelo Pedreira Miranda and Marcos Antonio Machado and Juliana Freitas-Astúa},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/1744-7917.12654 https://onlinelibrary.wiley.com/doi/abs/10.1111/1744-7917.12654 https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12654},
doi = {10.1111/1744-7917.12654},
issn = {1744-7917},
year = {2020},
date = {2020-01-01},
journal = {Insect Science},
volume = {27},
issue = {3},
pages = {519-530},
publisher = {John Wiley & Sons, Ltd},
abstract = {Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of “Candidatus Liberibacter spp.,” associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%–77% and 66%–90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.},
keywords = {Asian citrus psyllid, Citrus sinensis, Cry toxins, endophytic Bt, huanglongbing, young shoots},
pubstate = {published},
tppubtype = {article}
}
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of “Candidatus Liberibacter spp.,” associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%–77% and 66%–90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.
Galdeano, Diogo Manzano; Pacheco, Inaiara Souza; Alves, Gustavo Rodrigues; Granato, Laís Moreira; Rashidi, Mahnaz; Turner, Donielle; Levy, Amit; Machado, Marcos Antonio
Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri Journal Article
In: Tropical Plant Pathology, vol. 45, iss. 6, pp. 559-571, 2020, ISSN: 19832052.
Abstract | Links | BibTeX | Tags: ‘Candidatus Liberibacter asiaticus’, Asian citrus psyllid, Bacteria-vector interactions, huanglongbing, Multitrophic interaction, Vector-borne pathogens
@article{Galdeano2020,
title = {Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri},
author = {Diogo Manzano Galdeano and Inaiara Souza Pacheco and Gustavo Rodrigues Alves and Laís Moreira Granato and Mahnaz Rashidi and Donielle Turner and Amit Levy and Marcos Antonio Machado},
url = {https://link.springer.com/article/10.1007/s40858-020-00375-4},
doi = {10.1007/S40858-020-00375-4/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {559-571},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {Interactions between insects and plant pathogens have been more enthusiastically studied in the recent decade, especially those relationships which takes the insects as vectors. The spectrum of these interactions ranges from mutualistic to pathogenic. The length of the co-evolutionary process will determine whether a microorganism shares a friend or a foe relationship with its host, and a friendship connection is frequently observed if the coexistence is longer. This review updates knowledge about the morphological, physiological and genetic mechanisms that drive the interaction between ‘Candidatus Liberibacter asiaticus’ (Las) and its vector, the Asian citrus psyllid, Diaphorina citri. Las is the predominant causal agent of citrus huanglongbing (HLB) disease, the major constrain to citrus production worldwide. This bacterium is transmitted by D. citri, in a propagative-circulative manner during its feeding from plant host. Understanding of the interactions among vector, plant pathogen and host plant are important for the management of this vector-borne disease complex.},
keywords = {‘Candidatus Liberibacter asiaticus’, Asian citrus psyllid, Bacteria-vector interactions, huanglongbing, Multitrophic interaction, Vector-borne pathogens},
pubstate = {published},
tppubtype = {article}
}
Interactions between insects and plant pathogens have been more enthusiastically studied in the recent decade, especially those relationships which takes the insects as vectors. The spectrum of these interactions ranges from mutualistic to pathogenic. The length of the co-evolutionary process will determine whether a microorganism shares a friend or a foe relationship with its host, and a friendship connection is frequently observed if the coexistence is longer. This review updates knowledge about the morphological, physiological and genetic mechanisms that drive the interaction between ‘Candidatus Liberibacter asiaticus’ (Las) and its vector, the Asian citrus psyllid, Diaphorina citri. Las is the predominant causal agent of citrus huanglongbing (HLB) disease, the major constrain to citrus production worldwide. This bacterium is transmitted by D. citri, in a propagative-circulative manner during its feeding from plant host. Understanding of the interactions among vector, plant pathogen and host plant are important for the management of this vector-borne disease complex.
2018
Tomaseto, A. F.; Miranda, M. P.; Moral, R. A.; Lara, I. A. R.; Fereres, A.; Lopes, J. R. S.
Environmental conditions for Diaphorina citri Kuwayama (Hemiptera: Liviidae) take-off Journal Article
In: Journal of Applied Entomology, vol. 142, iss. 1-2, pp. 104-113, 2018, ISSN: 1439-0418.
Abstract | Links | BibTeX | Tags: Asian citrus psyllid, diel flight periodicity, flight propensity, huanglongbing, temperature
@article{Tomaseto2018,
title = {Environmental conditions for Diaphorina citri Kuwayama (Hemiptera: Liviidae) take-off},
author = {A. F. Tomaseto and M. P. Miranda and R. A. Moral and I. A. R. Lara and A. Fereres and J. R. S. Lopes},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jen.12418 https://onlinelibrary.wiley.com/doi/abs/10.1111/jen.12418 https://onlinelibrary.wiley.com/doi/10.1111/jen.12418},
doi = {10.1111/JEN.12418},
issn = {1439-0418},
year = {2018},
date = {2018-01-01},
journal = {Journal of Applied Entomology},
volume = {142},
issue = {1-2},
pages = {104-113},
publisher = {John Wiley & Sons, Ltd},
abstract = {Environmental factors that influence flight activity of Diaphorina citri Kuwayama (Hemiptera: Liviidae) may have implications for Huanglongbing spread and management. In this work, four studies were conducted to evaluate the effect of environmental conditions on D. citri take-off. In the first, insects were transferred to sweet orange seedlings and confined inside an acrylic cage to verify the take-off periodicity and the effect of environmental factors on this process. In the second, take-off temperature threshold was estimated by recording the number of insects that initiated flight from a platform when subjected to gradual temperature increases from 15 to 39°C. In the other studies, we evaluated the effect of different photoperiods and temperature regimes (third study) and of constant temperatures (fourth study) on the propensity for D. citri flight. Insects were confined in clear plastic bottle cages with tubes of 50 ml placed on the cab, to collect emerged adults that initiated flights. Results showed that a small portion of the tested population (maximum 10%) tends to take off from plants and this behaviour is more prevalent in the afternoon (14:00–16:00 h), coinciding with daytimes of lower humidity and higher thermal amplitude. Adults that were submitted to lower temperatures (18°C) and short light periods (10 h) showed less propensity to flight. In contrast, at constant 27°C, the insects were more prone to flight, and this result was confirmed when individuals were submitted to increases in temperature, indicating that 27.14°C is the take-off temperature threshold of D. citri. Results show that temperature plays an important role in the flight activity of D. citri and suggest that control measures of the insect may be more effective in the morning and in temperatures below 27°C, when the probability to take off from a host is lower.},
keywords = {Asian citrus psyllid, diel flight periodicity, flight propensity, huanglongbing, temperature},
pubstate = {published},
tppubtype = {article}
}
Environmental factors that influence flight activity of Diaphorina citri Kuwayama (Hemiptera: Liviidae) may have implications for Huanglongbing spread and management. In this work, four studies were conducted to evaluate the effect of environmental conditions on D. citri take-off. In the first, insects were transferred to sweet orange seedlings and confined inside an acrylic cage to verify the take-off periodicity and the effect of environmental factors on this process. In the second, take-off temperature threshold was estimated by recording the number of insects that initiated flight from a platform when subjected to gradual temperature increases from 15 to 39°C. In the other studies, we evaluated the effect of different photoperiods and temperature regimes (third study) and of constant temperatures (fourth study) on the propensity for D. citri flight. Insects were confined in clear plastic bottle cages with tubes of 50 ml placed on the cab, to collect emerged adults that initiated flights. Results showed that a small portion of the tested population (maximum 10%) tends to take off from plants and this behaviour is more prevalent in the afternoon (14:00–16:00 h), coinciding with daytimes of lower humidity and higher thermal amplitude. Adults that were submitted to lower temperatures (18°C) and short light periods (10 h) showed less propensity to flight. In contrast, at constant 27°C, the insects were more prone to flight, and this result was confirmed when individuals were submitted to increases in temperature, indicating that 27.14°C is the take-off temperature threshold of D. citri. Results show that temperature plays an important role in the flight activity of D. citri and suggest that control measures of the insect may be more effective in the morning and in temperatures below 27°C, when the probability to take off from a host is lower.