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2021
Mendes, Maria Inês Souza; Verde, Denise Santos Vila; Ramos, Andresa Priscila Souza; Gesteira, Abelmon Silva; Filho, Walter Santos Soares; Souza, Antônio Silva
In vitro conservation of citrus rootstocks using paclobutrazol and analysis of plant viability and genetic stability Journal Article
In: Scientia Horticulturae, vol. 286, pp. 110231, 2021, ISSN: 0304-4238.
Abstract | Links | BibTeX | Tags: Citrus, genetic resources, Growth retardant, Somaclonal variation, Tissue culture
@article{Mendes2021,
title = {In vitro conservation of citrus rootstocks using paclobutrazol and analysis of plant viability and genetic stability},
author = {Maria Inês Souza Mendes and Denise Santos Vila Verde and Andresa Priscila Souza Ramos and Abelmon Silva Gesteira and Walter Santos Soares Filho and Antônio Silva Souza},
doi = {10.1016/J.SCIENTA.2021.110231},
issn = {0304-4238},
year = {2021},
date = {2021-01-01},
journal = {Scientia Horticulturae},
volume = {286},
pages = {110231},
publisher = {Elsevier},
abstract = {The vulnerabilities to which citrus genetic resources are exposed under field conservation conditions make it important to ensure an in vitro backup. Thus, in this study, we aimed to establish a protocol for in vitro conservation of citrus rootstocks by analyzing the use of paclobutrazol to maintain a minimum growth and ensure the viability and genetic stability of conserved plants. In the first experiment, 1 cm-long apical microcuttings from plants of five rootstocks grown in vitro were placed in basal woody plant medium, supplemented with 0.2, 0.4, 0.6, 0.8, and 1.0 mg L−1 of paclobutrazol, and maintained in a conservation room at a temperature of 22 ± 1 °C, photoperiod of 12 h, and photon flux density of 20 μmol m−2 s−1 for 6 months. After this period, the plants from the medium selected for in vitro conservation of the rootstocks were acclimatized. For the second experiment, nucellar seedlings (confirmed by simple sequence repeat [SSR] markers) of 10 citrus rootstocks were multiplied and conserved in the previously defined medium. At the end of 12 months, the plants were subjected to three subculturing cycles to verify their viability. Molecular analyses were performed on plants from the third subculture using 21 SSR markers to detect somaclonal variants. Paclobutrazol was not efficient in maintaining reduced plant growth. Acclimatized plants grown in vitro exhibited 100% survival. SSR markers were efficient in characterizing the studied rootstocks. In general, the in vitro conservation conditions did not affect plant viability. The three subculturing cycles performed after conservation did not result in somaclonal variants. The conservation protocol described in this study can be used for the in vitro maintenance of the analyzed rootstocks and can be extended to other citrus genotypes.},
keywords = {Citrus, genetic resources, Growth retardant, Somaclonal variation, Tissue culture},
pubstate = {published},
tppubtype = {article}
}
The vulnerabilities to which citrus genetic resources are exposed under field conservation conditions make it important to ensure an in vitro backup. Thus, in this study, we aimed to establish a protocol for in vitro conservation of citrus rootstocks by analyzing the use of paclobutrazol to maintain a minimum growth and ensure the viability and genetic stability of conserved plants. In the first experiment, 1 cm-long apical microcuttings from plants of five rootstocks grown in vitro were placed in basal woody plant medium, supplemented with 0.2, 0.4, 0.6, 0.8, and 1.0 mg L−1 of paclobutrazol, and maintained in a conservation room at a temperature of 22 ± 1 °C, photoperiod of 12 h, and photon flux density of 20 μmol m−2 s−1 for 6 months. After this period, the plants from the medium selected for in vitro conservation of the rootstocks were acclimatized. For the second experiment, nucellar seedlings (confirmed by simple sequence repeat [SSR] markers) of 10 citrus rootstocks were multiplied and conserved in the previously defined medium. At the end of 12 months, the plants were subjected to three subculturing cycles to verify their viability. Molecular analyses were performed on plants from the third subculture using 21 SSR markers to detect somaclonal variants. Paclobutrazol was not efficient in maintaining reduced plant growth. Acclimatized plants grown in vitro exhibited 100% survival. SSR markers were efficient in characterizing the studied rootstocks. In general, the in vitro conservation conditions did not affect plant viability. The three subculturing cycles performed after conservation did not result in somaclonal variants. The conservation protocol described in this study can be used for the in vitro maintenance of the analyzed rootstocks and can be extended to other citrus genotypes.
2018
Brito, Marcos E. B.; Soares, Lauriane A. A.; Filho, Walter S. Soares; Fernandes, Pedro D.; Silva, Elaine C. B.; Sá, Francisco V. S.; Silva, Luderlândio A.
Emergence and morphophysiology of Sunki mandarin and other citrus genotypes seedlings under saline stress Journal Article
In: Spanish Journal of Agricultural Research, vol. 16, iss. 1, pp. e0801-e0801, 2018, ISSN: 2171-9292.
Abstract | Links | BibTeX | Tags: agriculture, breeding, Citrus spp., diseases, economics, fertilization, genetic resources, genetics, growth, irrigation, livestock, pests, photosynthesis, physiology, reproduction, rootstock, Salinity, soils, water manage, weeds
@article{Brito2018,
title = {Emergence and morphophysiology of Sunki mandarin and other citrus genotypes seedlings under saline stress},
author = {Marcos E. B. Brito and Lauriane A. A. Soares and Walter S. Soares Filho and Pedro D. Fernandes and Elaine C. B. Silva and Francisco V. S. Sá and Luderlândio A. Silva},
url = {https://revistas.inia.es/index.php/sjar/article/view/9400/3932 https://revistas.inia.es/index.php/sjar/article/view/9400},
doi = {10.5424/SJAR/2018161-9400},
issn = {2171-9292},
year = {2018},
date = {2018-01-01},
journal = {Spanish Journal of Agricultural Research},
volume = {16},
issue = {1},
pages = {e0801-e0801},
publisher = {Ministerio de Agricultura Pesca y Alimentacion},
abstract = {The scarcity of good quality water is a limiting factor for irrigated agriculture, especially in arid and semiarid regions, where water sources generally contain high concentrations of ions. In such conditions, it is essential to cultivate genotypes with economic potential and tolerance to salinity. Considering the importance of citrus and the need to identify genetic materials that adapt to saline stress, this study evaluates the salinity tolerance of 10 genotypes with rootstock potential. For the irrigation water, five levels of electrical conductivity (ECw: 0.8, 1.6, 2.4, 3.2 and 4.0 dS/m at 25 °C) were used in a randomized block design with three replications, and seed germination and growth variables, as well as physiological plant parameters, were evaluated. The germination rate of the hybrid TSKC × CTARG – 019 was the best under salt conditions, as it was the genotype with the highest tolerance to salinity in the germination stage. Salinity reduced the growth of the citrus genotypes, with the possibility of using water with EC of up to 1.6 dS/m in the rootstock formation stage. Salt stress affected the photosynthetic rate of the genotypes ‘San Diego’ citrandarin and TSKC × CTSW – 018 by reducing the stomatal conductance, restricting the diffusion of CO2 into the substomatal camera. The genotypes TSKC × CTARG – 019, TSKC × CTTR – 012 and TSKC × TRBK – 007 have the greatest potential for growth and photosynthetic apparatus efficiency when subjected to salinity.},
keywords = {agriculture, breeding, Citrus spp., diseases, economics, fertilization, genetic resources, genetics, growth, irrigation, livestock, pests, photosynthesis, physiology, reproduction, rootstock, Salinity, soils, water manage, weeds},
pubstate = {published},
tppubtype = {article}
}
The scarcity of good quality water is a limiting factor for irrigated agriculture, especially in arid and semiarid regions, where water sources generally contain high concentrations of ions. In such conditions, it is essential to cultivate genotypes with economic potential and tolerance to salinity. Considering the importance of citrus and the need to identify genetic materials that adapt to saline stress, this study evaluates the salinity tolerance of 10 genotypes with rootstock potential. For the irrigation water, five levels of electrical conductivity (ECw: 0.8, 1.6, 2.4, 3.2 and 4.0 dS/m at 25 °C) were used in a randomized block design with three replications, and seed germination and growth variables, as well as physiological plant parameters, were evaluated. The germination rate of the hybrid TSKC × CTARG – 019 was the best under salt conditions, as it was the genotype with the highest tolerance to salinity in the germination stage. Salinity reduced the growth of the citrus genotypes, with the possibility of using water with EC of up to 1.6 dS/m in the rootstock formation stage. Salt stress affected the photosynthetic rate of the genotypes ‘San Diego’ citrandarin and TSKC × CTSW – 018 by reducing the stomatal conductance, restricting the diffusion of CO2 into the substomatal camera. The genotypes TSKC × CTARG – 019, TSKC × CTTR – 012 and TSKC × TRBK – 007 have the greatest potential for growth and photosynthetic apparatus efficiency when subjected to salinity.