Publications

@article{Bezerra2021,

title = {How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters},

author = {Yane Caroline Dos Anjos Bezerra and João Paulo Rodrigues Marques and Liliane Cristina Liborio Stipp and Lísia Borges Attílio and Juliana Freitas-Astúa and Francisco Assis Alves Mourão Filho},

url = {http://www.scielo.br/j/rbf/a/tg5tQ8p8CcRK7xKDDcHCTjF/?lang=en},

doi = {10.1590/0100-29452021005},

issn = {0100-2945},

year  = {2021},

date = {2021-01-01},

journal = {Revista Brasileira de Fruticultura},

volume = {43},

issue = {4},

publisher = {Sociedade Brasileira de Fruticultura},

abstract = {Abstract New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ‘Carrizo’ citrange [ (Poncirus trifoliataL.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (ß-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ‘Rangpur’ lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissue-specific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.},

keywords = {Candidatus Liberibacter spp., disease resistance, genetic transformation, GUS, histolocalization},

pubstate = {published},

tppubtype = {article}

}

@article{Tavano2018,

title = {Sweet orange genetic transformation with the attacin A gene under the control of phloem-specific promoters and inoculation with Candidatus Liberibacter asiaticus},

author = {Eveline Carla Rocha Tavano and Ligia Erpen and Bianca Aluisi and Ricardo Harakava and João Roberto Spotti Lopes and Maria Lucia Carneiro Vieira and Sônia Maria De Stefano Piedade and Beatriz Madalena Januzzi Mendes and Francisco Assis Alves Mourão Filho},

url = {https://www.tandfonline.com/doi/abs/10.1080/14620316.2018.1493361},

doi = {10.1080/14620316.2018.1493361},

issn = {14620316},

year  = {2018},

date = {2018-01-01},

journal = {https://doi.org/10.1080/14620316.2018.1493361},

volume = {94},

issue = {2},

pages = {210-219},

publisher = {Taylor & Francis},

abstract = {An alternative approach to control Huanglongbing disease in Citrus is the development of transgenic plants expressing genes that may influence pathogen development. We report herein the production ...},

keywords = {antimicrobial peptide, Bacteria, Citrus sinensis, disease resistance, Gene expression, huanglongbing},

pubstate = {published},

tppubtype = {article}

}