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2017
Miyata, Luzia Yuriko; Harakava, Ricardo; Attílio, Lísia Borges; Mendes, Beatriz Madalena Januzzi; Lopes, João Roberto Spotti; Coletta-Filho, Helvécio Della; Souza, Alessandra Alves De; Filho, Francisco De Assis Alves Mourão
PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus Journal Article
In: Revista Brasileira de Fruticultura, vol. 39, iss. 3, 2017, ISSN: 0100-2945.
Abstract | Links | BibTeX | Tags: Citrus sinensis, Diaphorina citri, genetic transformation, GUS, huanglongbing, quantitative real, time PCR
@article{Miyata2017,
title = {PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY \textit{Candidatus} Liberibacter asiaticus},
author = {Luzia Yuriko Miyata and Ricardo Harakava and Lísia Borges Attílio and Beatriz Madalena Januzzi Mendes and João Roberto Spotti Lopes and Helvécio Della Coletta-Filho and Alessandra Alves De Souza and Francisco De Assis Alves Mourão Filho},
url = {http://www.scielo.br/j/rbf/a/NFKjGmNsP48jRtP7txqqcFh/?lang=en},
doi = {10.1590/0100-29452017993},
issn = {0100-2945},
year = {2017},
date = {2017-01-01},
journal = {Revista Brasileira de Fruticultura},
volume = {39},
issue = {3},
publisher = {Sociedade Brasileira de Fruticultura},
abstract = {ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las.},
keywords = {Citrus sinensis, Diaphorina citri, genetic transformation, GUS, huanglongbing, quantitative real, time PCR},
pubstate = {published},
tppubtype = {article}
}
ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants (Citrus sinensis L. Osbeck) transformed with the uidA (GUS) reporter gene under the control of AtPP2, AtSUC2 and CsPP2 promoters were infected to evaluate the interdependence between transgene expression and the concentration of Las. Plants were inoculated with Las by Diaphorina citri and eighteen months later, bacterial concentration and uidA expression were determined by qPCR and RT-qPCR, respectively. Reporter gene expression driven by AtSUC2 promoter was strongly and positively correlated with Las concentration. Therefore, this promoter combines desirable features of both tissue-specificity and pathogen-inducibility for the production of transgenic plants tolerant to Las.