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2020
Schinor, Evandro Henrique; Arakaki, Marcelo; Cristofani-Yaly, Mariângela
Agronomic characterization of citrandarin fruits and seeds Journal Article
In: Comunicata Scientiae, vol. 11, pp. e3416-e3416, 2020, ISSN: 2177-5133.
Abstract | Links | BibTeX | Tags: Citrus sunki, hybrids, Polyembryony, Poncirus trifoliata, rootstock
@article{Schinor2020,
title = {Agronomic characterization of citrandarin fruits and seeds},
author = {Evandro Henrique Schinor and Marcelo Arakaki and Mariângela Cristofani-Yaly},
url = {https://www.comunicatascientiae.com.br/comunicata/article/view/3416},
doi = {10.14295/CS.V11I.3416},
issn = {2177-5133},
year = {2020},
date = {2020-01-01},
journal = {Comunicata Scientiae},
volume = {11},
pages = {e3416-e3416},
publisher = {Federal University ofPiauI},
abstract = {In citrus cultivation, rootstocks are of fundamental importance and affect several characteristics of the variety used as canopy. Despite the great diversity within Citrus and related genera, the production of rootstocks in Brazil is restricted to a small number of varieties, making the citrus culture vulnerable to the appearance of phytosanitary problems. The aim of this study was to agronomically characterize fruits and seeds of seven citrandarins [Citrus sunki (Hayata) hort. ex Tanaka x Poncirus trifoliata cv. Rubidoux (L.) Raf.], obtained by controlled crossing. The orchard was installed in randomized blocks, with three replicates, in the municipality of Cordeirópolis, SP, where 20 fruits were collected in each replicate, obtained from free pollination of seven citrandarins, Swingle citrumelo and Rangpur lime. The following variables were evaluated: fruit mass, height, diameter, total number of seeds and percentage of viable seeds per fruit; number of embryos per seed, mass of one thousand seeds, number of seeds in 1.0 kg, final emergence rate, number of seedlings per seed, polyembryony rate, emergence speed index and seedling height at 60 days after sowing. For fruit size, the highest values were obtained for Swingle citrumelo. For number of embryos per seed, seedlings obtained through seed and polyembryony, citrandarin TSxPT 245 showed the highest values. Although citrandarin fruits had smaller size than fruits from commercial Rangpur lime and Swingle citrumelo rootstocks, characteristics related to seeds such as viability, polyembryony and emergence rate, were similar or superior, and can be considered potential new rootstocks for the production of citrus plants.},
keywords = {Citrus sunki, hybrids, Polyembryony, Poncirus trifoliata, rootstock},
pubstate = {published},
tppubtype = {article}
}
In citrus cultivation, rootstocks are of fundamental importance and affect several characteristics of the variety used as canopy. Despite the great diversity within Citrus and related genera, the production of rootstocks in Brazil is restricted to a small number of varieties, making the citrus culture vulnerable to the appearance of phytosanitary problems. The aim of this study was to agronomically characterize fruits and seeds of seven citrandarins [Citrus sunki (Hayata) hort. ex Tanaka x Poncirus trifoliata cv. Rubidoux (L.) Raf.], obtained by controlled crossing. The orchard was installed in randomized blocks, with three replicates, in the municipality of Cordeirópolis, SP, where 20 fruits were collected in each replicate, obtained from free pollination of seven citrandarins, Swingle citrumelo and Rangpur lime. The following variables were evaluated: fruit mass, height, diameter, total number of seeds and percentage of viable seeds per fruit; number of embryos per seed, mass of one thousand seeds, number of seeds in 1.0 kg, final emergence rate, number of seedlings per seed, polyembryony rate, emergence speed index and seedling height at 60 days after sowing. For fruit size, the highest values were obtained for Swingle citrumelo. For number of embryos per seed, seedlings obtained through seed and polyembryony, citrandarin TSxPT 245 showed the highest values. Although citrandarin fruits had smaller size than fruits from commercial Rangpur lime and Swingle citrumelo rootstocks, characteristics related to seeds such as viability, polyembryony and emergence rate, were similar or superior, and can be considered potential new rootstocks for the production of citrus plants.
2018
Lima, Rômulo P. M.; Curtolo, Maiara; Merfa, Marcus V.; Cristofani-Yaly, Mariângela; Machado, Marcos A.
QTLs and eQTLs mapping related to citrandarins' resistance to citrus gummosis disease Journal Article
In: BMC Genomics, vol. 19, iss. 1, pp. 1-17, 2018, ISSN: 14712164.
Abstract | Links | BibTeX | Tags: Citrus sunki, Defense genes, Expression quantitative trait loci, hybrids, Poncirus trifoliata
@article{Lima2018b,
title = {QTLs and eQTLs mapping related to citrandarins' resistance to citrus gummosis disease},
author = {Rômulo P. M. Lima and Maiara Curtolo and Marcus V. Merfa and Mariângela Cristofani-Yaly and Marcos A. Machado},
url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4888-2},
doi = {10.1186/S12864-018-4888-2/TABLES/4},
issn = {14712164},
year = {2018},
date = {2018-01-01},
journal = {BMC Genomics},
volume = {19},
issue = {1},
pages = {1-17},
publisher = {BioMed Central Ltd.},
abstract = {Background: Phytophthora nicotianae Breda de Haan (Phytophthora parasitica Dastur) causes severe damage to citrus crops worldwide. A population of citrandarins was created from the cross between the susceptible parent Citrus sunki Hort. Ex Tan. and the resistant parent Poncirus trifoliata (L.) Raf. cv. Rubidoux, both parents and two reference rootstocks (Rangpur lime and Swingle citrumelo) were grafted in a greenhouse on Rangpur lime. Inoculations were performed at 10 cm and 15 cm above the grafting region and the resulting lesions were evaluated by measuring the lesion length 60 days after inoculation. As control, non-inoculated plants of each genotype were used. In addition, we evaluated the expression of 19 candidate genes involved in citrus defense response 48 h after pathogen infection by quantitative Real-Time PCR (qPCR). We mapped genomic regions of Quantitative Trait Loci (QTLs) and Expression Quantitative Trait Loci (eQTLs) associated with resistance to P. parasitica in the linkage groups (LGs) of the previously constructed maps of C. sunki and P. trifoliata. Results: We found disease severity differences among the generated hybrids, with lesion lengths varying from 1.15 to 11.13 mm. The heritability of the character was 65%. These results indicate that there is a great possibility of success in the selection of resistant hybrids within this experiment. The analysis of gene expression profile demonstrated a great variation of responses regarding the activation of plant defense pathways, indicating that citrandarins have several defense strategies to control oomycete infection. The information of the phenotypic and gene expression data made possible to detect genomic regions associated with resistance. Three QTLs and 84 eQTLs were detected in the linkage map of P. trifoliata, while one QTL and 110 eQTLs were detected in C. sunki. Conclusions: This is the first study to use eQTLs mapping in the Phytophthora-citrus interaction. Our results from the QTLs and eQTLs mapping allow us to conclude that the resistance of some citrandarins to the infection by P. parasitica is due to a favorable combination of QTLs and eQTLs transmitted by both parents.},
keywords = {Citrus sunki, Defense genes, Expression quantitative trait loci, hybrids, Poncirus trifoliata},
pubstate = {published},
tppubtype = {article}
}
Background: Phytophthora nicotianae Breda de Haan (Phytophthora parasitica Dastur) causes severe damage to citrus crops worldwide. A population of citrandarins was created from the cross between the susceptible parent Citrus sunki Hort. Ex Tan. and the resistant parent Poncirus trifoliata (L.) Raf. cv. Rubidoux, both parents and two reference rootstocks (Rangpur lime and Swingle citrumelo) were grafted in a greenhouse on Rangpur lime. Inoculations were performed at 10 cm and 15 cm above the grafting region and the resulting lesions were evaluated by measuring the lesion length 60 days after inoculation. As control, non-inoculated plants of each genotype were used. In addition, we evaluated the expression of 19 candidate genes involved in citrus defense response 48 h after pathogen infection by quantitative Real-Time PCR (qPCR). We mapped genomic regions of Quantitative Trait Loci (QTLs) and Expression Quantitative Trait Loci (eQTLs) associated with resistance to P. parasitica in the linkage groups (LGs) of the previously constructed maps of C. sunki and P. trifoliata. Results: We found disease severity differences among the generated hybrids, with lesion lengths varying from 1.15 to 11.13 mm. The heritability of the character was 65%. These results indicate that there is a great possibility of success in the selection of resistant hybrids within this experiment. The analysis of gene expression profile demonstrated a great variation of responses regarding the activation of plant defense pathways, indicating that citrandarins have several defense strategies to control oomycete infection. The information of the phenotypic and gene expression data made possible to detect genomic regions associated with resistance. Three QTLs and 84 eQTLs were detected in the linkage map of P. trifoliata, while one QTL and 110 eQTLs were detected in C. sunki. Conclusions: This is the first study to use eQTLs mapping in the Phytophthora-citrus interaction. Our results from the QTLs and eQTLs mapping allow us to conclude that the resistance of some citrandarins to the infection by P. parasitica is due to a favorable combination of QTLs and eQTLs transmitted by both parents.
Lima, Rômulo P. M.; Curtolo, Maiara; Merfa, Marcus V.; Cristofani-Yaly, Mariângela; Machado, Marcos A.
QTLs and eQTLs mapping related to citrandarins' resistance to citrus gummosis disease Journal Article
In: BMC Genomics, vol. 19, iss. 1, pp. 1-17, 2018, ISSN: 14712164.
Abstract | Links | BibTeX | Tags: Citrus sunki, Defense genes, Expression quantitative trait loci, hybrids, Poncirus trifoliata
@article{Lima2018,
title = {QTLs and eQTLs mapping related to citrandarins' resistance to citrus gummosis disease},
author = {Rômulo P. M. Lima and Maiara Curtolo and Marcus V. Merfa and Mariângela Cristofani-Yaly and Marcos A. Machado},
url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4888-2},
doi = {10.1186/S12864-018-4888-2/TABLES/4},
issn = {14712164},
year = {2018},
date = {2018-01-01},
journal = {BMC Genomics},
volume = {19},
issue = {1},
pages = {1-17},
publisher = {BioMed Central Ltd.},
abstract = {Background: Phytophthora nicotianae Breda de Haan (Phytophthora parasitica Dastur) causes severe damage to citrus crops worldwide. A population of citrandarins was created from the cross between the susceptible parent Citrus sunki Hort. Ex Tan. and the resistant parent Poncirus trifoliata (L.) Raf. cv. Rubidoux, both parents and two reference rootstocks (Rangpur lime and Swingle citrumelo) were grafted in a greenhouse on Rangpur lime. Inoculations were performed at 10 cm and 15 cm above the grafting region and the resulting lesions were evaluated by measuring the lesion length 60 days after inoculation. As control, non-inoculated plants of each genotype were used. In addition, we evaluated the expression of 19 candidate genes involved in citrus defense response 48 h after pathogen infection by quantitative Real-Time PCR (qPCR). We mapped genomic regions of Quantitative Trait Loci (QTLs) and Expression Quantitative Trait Loci (eQTLs) associated with resistance to P. parasitica in the linkage groups (LGs) of the previously constructed maps of C. sunki and P. trifoliata. Results: We found disease severity differences among the generated hybrids, with lesion lengths varying from 1.15 to 11.13 mm. The heritability of the character was 65%. These results indicate that there is a great possibility of success in the selection of resistant hybrids within this experiment. The analysis of gene expression profile demonstrated a great variation of responses regarding the activation of plant defense pathways, indicating that citrandarins have several defense strategies to control oomycete infection. The information of the phenotypic and gene expression data made possible to detect genomic regions associated with resistance. Three QTLs and 84 eQTLs were detected in the linkage map of P. trifoliata, while one QTL and 110 eQTLs were detected in C. sunki. Conclusions: This is the first study to use eQTLs mapping in the Phytophthora-citrus interaction. Our results from the QTLs and eQTLs mapping allow us to conclude that the resistance of some citrandarins to the infection by P. parasitica is due to a favorable combination of QTLs and eQTLs transmitted by both parents.},
keywords = {Citrus sunki, Defense genes, Expression quantitative trait loci, hybrids, Poncirus trifoliata},
pubstate = {published},
tppubtype = {article}
}
Background: Phytophthora nicotianae Breda de Haan (Phytophthora parasitica Dastur) causes severe damage to citrus crops worldwide. A population of citrandarins was created from the cross between the susceptible parent Citrus sunki Hort. Ex Tan. and the resistant parent Poncirus trifoliata (L.) Raf. cv. Rubidoux, both parents and two reference rootstocks (Rangpur lime and Swingle citrumelo) were grafted in a greenhouse on Rangpur lime. Inoculations were performed at 10 cm and 15 cm above the grafting region and the resulting lesions were evaluated by measuring the lesion length 60 days after inoculation. As control, non-inoculated plants of each genotype were used. In addition, we evaluated the expression of 19 candidate genes involved in citrus defense response 48 h after pathogen infection by quantitative Real-Time PCR (qPCR). We mapped genomic regions of Quantitative Trait Loci (QTLs) and Expression Quantitative Trait Loci (eQTLs) associated with resistance to P. parasitica in the linkage groups (LGs) of the previously constructed maps of C. sunki and P. trifoliata. Results: We found disease severity differences among the generated hybrids, with lesion lengths varying from 1.15 to 11.13 mm. The heritability of the character was 65%. These results indicate that there is a great possibility of success in the selection of resistant hybrids within this experiment. The analysis of gene expression profile demonstrated a great variation of responses regarding the activation of plant defense pathways, indicating that citrandarins have several defense strategies to control oomycete infection. The information of the phenotypic and gene expression data made possible to detect genomic regions associated with resistance. Three QTLs and 84 eQTLs were detected in the linkage map of P. trifoliata, while one QTL and 110 eQTLs were detected in C. sunki. Conclusions: This is the first study to use eQTLs mapping in the Phytophthora-citrus interaction. Our results from the QTLs and eQTLs mapping allow us to conclude that the resistance of some citrandarins to the infection by P. parasitica is due to a favorable combination of QTLs and eQTLs transmitted by both parents.