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Check the list of papers from NScTI Citrus
Use the filters at the top of the list to select articles by year or by researcher. In the box for each article, use the links ("Summary", "Links", "BibTeX") to open additional information.
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2021
Moura, Vanessa S.; Pollettini, Flávia L.; Ferraz, Luriany P.; Mazzi, Maurício V.; Kupper, Katia C.
Purification of a killer toxin from Aureobasidium pullulans for the biocontrol of phytopathogens Journal Article
In: Journal of Basic Microbiology, vol. 61, iss. 2, pp. 77-87, 2021, ISSN: 1521-4028.
Abstract | Links | BibTeX | Tags: aurantii, Geotrichum citri, Hydrolytic enzymes, like, Penicillium digitatum, ubiquitin
@article{Moura2021,
title = {Purification of a killer toxin from Aureobasidium pullulans for the biocontrol of phytopathogens},
author = {Vanessa S. Moura and Flávia L. Pollettini and Luriany P. Ferraz and Maurício V. Mazzi and Katia C. Kupper},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/jobm.202000164 https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.202000164 https://onlinelibrary.wiley.com/doi/10.1002/jobm.202000164},
doi = {10.1002/JOBM.202000164},
issn = {1521-4028},
year = {2021},
date = {2021-01-01},
journal = {Journal of Basic Microbiology},
volume = {61},
issue = {2},
pages = {77-87},
publisher = {John Wiley & Sons, Ltd},
abstract = {The objectives of the present study were to purify and assess the killer toxin effect produced by Aureobasidium pullulans under casual agents of green mold (Penicillum digitatum) and sour rot (Geotrichum citri-aurantii). Initially, different methods of protein precipitation were tested. The proteolytic activity and the presence of proteins acting on cell wall receptors, β-1,3-glucanase and chitinase were determined, and toxin purification was conducted by Sephadex G-75 gel exclusion chromatography and cellulose chromatography (medium fibers). Subsequently, purification was confirmed by polyacrylamide gel electrophoresis, and the detection of killer activity was performed in solid YEPD-methylene blue buffered with citrate-phosphate (0.1 M, pH 4.6). Toxin identification was performed by liquid chromatography–mass spectrometry. The results showed that the best protein precipitation method was 2:1 ethanol (vol/vol ethanol/supernatant). It was possible to observe the presence of enzymes with proteolytic activity, including β-1,3-glucanase and chitinase. During the purification process, it was verified that the killer toxin produced by the yeast has a low-molecular-weight protein belonging to the ubiquitin family, which presents killer activity against P. digitatum and G. citri-aurantii.},
keywords = {aurantii, Geotrichum citri, Hydrolytic enzymes, like, Penicillium digitatum, ubiquitin},
pubstate = {published},
tppubtype = {article}
}
The objectives of the present study were to purify and assess the killer toxin effect produced by Aureobasidium pullulans under casual agents of green mold (Penicillum digitatum) and sour rot (Geotrichum citri-aurantii). Initially, different methods of protein precipitation were tested. The proteolytic activity and the presence of proteins acting on cell wall receptors, β-1,3-glucanase and chitinase were determined, and toxin purification was conducted by Sephadex G-75 gel exclusion chromatography and cellulose chromatography (medium fibers). Subsequently, purification was confirmed by polyacrylamide gel electrophoresis, and the detection of killer activity was performed in solid YEPD-methylene blue buffered with citrate-phosphate (0.1 M, pH 4.6). Toxin identification was performed by liquid chromatography–mass spectrometry. The results showed that the best protein precipitation method was 2:1 ethanol (vol/vol ethanol/supernatant). It was possible to observe the presence of enzymes with proteolytic activity, including β-1,3-glucanase and chitinase. During the purification process, it was verified that the killer toxin produced by the yeast has a low-molecular-weight protein belonging to the ubiquitin family, which presents killer activity against P. digitatum and G. citri-aurantii.
2018
Cunha, Tatiane; Ferraz, Luriany Pompeo; Wehr, Pitt Paul; Kupper, Katia Cristina
Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum Journal Article
In: International Journal of Food Microbiology, vol. 276, pp. 20-27, 2018, ISSN: 0168-1605.
Abstract | Links | BibTeX | Tags: ‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists
@article{nokey,
title = {Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum},
author = {Tatiane Cunha and Luriany Pompeo Ferraz and Pitt Paul Wehr and Katia Cristina Kupper},
doi = {10.1016/J.IJFOODMICRO.2018.03.019},
issn = {0168-1605},
year = {2018},
date = {2018-01-01},
journal = {International Journal of Food Microbiology},
volume = {276},
pages = {20-27},
publisher = {Elsevier},
abstract = {Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.},
keywords = {‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists},
pubstate = {published},
tppubtype = {article}
}
Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.
Cunha, Tatiane; Ferraz, Luriany Pompeo; Wehr, Pitt Paul; Kupper, Katia Cristina
Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum Journal Article
In: International Journal of Food Microbiology, vol. 276, pp. 20-27, 2018, ISSN: 0168-1605.
Abstract | Links | BibTeX | Tags: ‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists
@article{nokey,
title = {Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum},
author = {Tatiane Cunha and Luriany Pompeo Ferraz and Pitt Paul Wehr and Katia Cristina Kupper},
doi = {10.1016/J.IJFOODMICRO.2018.03.019},
issn = {0168-1605},
year = {2018},
date = {2018-01-01},
journal = {International Journal of Food Microbiology},
volume = {276},
pages = {20-27},
publisher = {Elsevier},
abstract = {Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.},
keywords = {‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists},
pubstate = {published},
tppubtype = {article}
}
Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.
Cunha, Tatiane; Ferraz, Luriany Pompeo; Wehr, Pitt Paul; Kupper, Katia Cristina
Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum Journal Article
In: International Journal of Food Microbiology, vol. 276, pp. 20-27, 2018, ISSN: 0168-1605.
Abstract | Links | BibTeX | Tags: ‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists
@article{nokey,
title = {Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum},
author = {Tatiane Cunha and Luriany Pompeo Ferraz and Pitt Paul Wehr and Katia Cristina Kupper},
doi = {10.1016/J.IJFOODMICRO.2018.03.019},
issn = {0168-1605},
year = {2018},
date = {2018-01-01},
journal = {International Journal of Food Microbiology},
volume = {276},
pages = {20-27},
publisher = {Elsevier},
abstract = {Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.},
keywords = {‘Killer’ factor, Blue mold, Citrus sinensis, Hydrolytic enzymes, Yeast antagonists},
pubstate = {published},
tppubtype = {article}
}
Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The ‘killer’ activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the ‘killer’ activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.