Publications

@article{Soriano2019,

title = {\textit{In vitro} organogenesis and genetic transformation of mandarin cultivars},

author = {Leonardo Soriano and Eveline Carla Rocha Tavano and Marcelo Favaretto Correa and Ricardo Harakava and Beatriz Madalena Januzzi Mendes and Francisco Assis Alves Mourão Filho},

url = {http://www.scielo.br/j/rbf/a/F4kR35Gb5DgqT85x93mK39z/?lang=en},

doi = {10.1590/0100-29452019116},

issn = {0100-2945},

year  = {2019},

date = {2019-01-01},

journal = {Revista Brasileira de Fruticultura},

volume = {41},

issue = {2},

publisher = {Sociedade Brasileira de Fruticultura},

abstract = {Abstract The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.},

keywords = {antimicrobial peptide, Cultivar improvement, cytokinin, huanglongbing, transgenic plants},

pubstate = {published},

tppubtype = {article}

}

@article{Erpen2018,

title = {Isolation, characterization, and evaluation of three Citrus sinensis-derived constitutive gene promoters},

author = {L. Erpen and E. C. R. Tavano and R. Harakava and M. Dutt and J. W. Grosser and S. M. S. Piedade and B. M. J. Mendes and F. A. A. Mourão Filho},

url = {https://link.springer.com/article/10.1007/s00299-018-2298-1},

doi = {10.1007/S00299-018-2298-1/METRICS},

issn = {07217714},

year  = {2018},

date = {2018-01-01},

journal = {Plant Cell Reports},

volume = {37},

issue = {8},

pages = {1113-1125},

publisher = {Springer Verlag},

abstract = {Key message: Regulatory sequences from the citrus constitutive genes cyclophilin (CsCYP), glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha (CsEF1) were isolated, fused to the uidA gene, and qualitatively and quantitatively evaluated in transgenic sweet orange plants. Abstract: The 5′ upstream region of a gene (the promoter) is the most important component for the initiation and regulation of gene transcription of both native genes and transgenes in plants. The isolation and characterization of gene regulatory sequences are essential to the development of intragenic or cisgenic genetic manipulation strategies, which imply the use of genetic material from the same species or from closely related species. We describe herein the isolation and evaluation of the promoter sequence from three constitutively expressed citrus genes: cyclophilin (CsCYP), glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha (CsEF1). The functionality of the promoters was confirmed by a histochemical GUS assay in leaves, stems, and roots of stably transformed citrus plants expressing the promoter-uidA construct. Lower uidA mRNA levels were detected when the transgene was under the control of citrus promoters as compared to the expression under the control of the CaMV35S promoter. The association of the uidA gene with the citrus-derived promoters resulted in mRNA levels of up to 60–41.8% of the value obtained with the construct containing CaMV35S driving the uidA gene. Moreover, a lower inter-individual variability in transgene expression was observed amongst the different transgenic lines, where gene constructs containing citrus-derived promoters were used. In silico analysis of the citrus-derived promoter sequences revealed that their activity may be controlled by several putative cis-regulatory elements. These citrus promoters will expand the availability of regulatory sequences for driving gene expression in citrus gene-modification programs.},

keywords = {Cis-regulatory elements, Cultivar improvement, Gene expression, sweet orange},

pubstate = {published},

tppubtype = {article}

}