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Check the list of papers from NScTI Citrus
Use the filters at the top of the list to select articles by year or by researcher. In the box for each article, use the links ("Summary", "Links", "BibTeX") to open additional information.
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2020
Pacheco, Inaiara Souza; Galdeano, Diogo Manzano; Maluta, Nathalie Kristine Prado; Lopes, Joao Roberto Spotti; Machado, Marcos Antonio
Gene silencing of Diaphorina citri candidate effectors promotes changes in feeding behaviors Journal Article
In: Scientific Reports 2020 10:1, vol. 10, iss. 1, pp. 1-14, 2020, ISSN: 2045-2322.
Abstract | Links | BibTeX | Tags: Entomology, RNAi
@article{Pacheco2020,
title = {Gene silencing of Diaphorina citri candidate effectors promotes changes in feeding behaviors},
author = {Inaiara Souza Pacheco and Diogo Manzano Galdeano and Nathalie Kristine Prado Maluta and Joao Roberto Spotti Lopes and Marcos Antonio Machado},
url = {https://www.nature.com/articles/s41598-020-62856-5},
doi = {10.1038/s41598-020-62856-5},
issn = {2045-2322},
year = {2020},
date = {2020-01-01},
journal = {Scientific Reports 2020 10:1},
volume = {10},
issue = {1},
pages = {1-14},
publisher = {Nature Publishing Group},
abstract = {Insect effectors are mainly secreted by salivary glands, modulate plant physiology and favor the establishment and transmission of pathogens. Feeding is the principal vehicle of transmission of Candidatus Liberibacter asiaticus (Ca. Las) by the Asian citrus psyllid (ACP), Diaphorina citri. This study aimed to predict putative ACP effectors that may act on the Huanglongbing (HLB) pathosystem. Bioinformatics analysis led to the identification of 131 candidate effectors. Gene expression investigations were performed to select genes that were overexpressed in the ACP head and modulated by Ca. Las. To evaluate the actions of candidate effectors on D. citri feeding, six effectors were selected for gene silencing bioassays. Double-stranded RNAs (dsRNAs) of the target genes were delivered to D. citri adults via artificial diets for five days. RNAi silencing caused a reduction in the ACP lifespan and decreased the salivary sheath size and honeydew production. Moreover, after dsRNA delivery of the target genes using artificial diet, the feeding behaviors of the insects were evaluated on young leaves from citrus seedlings. These analyses proved that knockdown of D. citri effectors also interfered with ACP feeding abilities in planta, causing a decrease in honeydew production and reducing ACP survival. Electrical penetration graph (EPG) analysis confirmed the actions of the effectors on D. citri feeding behaviors. These results indicate that gene silencing of D. citri effectors may cause changes in D. citri feeding behaviors and could potentially be used for ACP control.},
keywords = {Entomology, RNAi},
pubstate = {published},
tppubtype = {article}
}
Insect effectors are mainly secreted by salivary glands, modulate plant physiology and favor the establishment and transmission of pathogens. Feeding is the principal vehicle of transmission of Candidatus Liberibacter asiaticus (Ca. Las) by the Asian citrus psyllid (ACP), Diaphorina citri. This study aimed to predict putative ACP effectors that may act on the Huanglongbing (HLB) pathosystem. Bioinformatics analysis led to the identification of 131 candidate effectors. Gene expression investigations were performed to select genes that were overexpressed in the ACP head and modulated by Ca. Las. To evaluate the actions of candidate effectors on D. citri feeding, six effectors were selected for gene silencing bioassays. Double-stranded RNAs (dsRNAs) of the target genes were delivered to D. citri adults via artificial diets for five days. RNAi silencing caused a reduction in the ACP lifespan and decreased the salivary sheath size and honeydew production. Moreover, after dsRNA delivery of the target genes using artificial diet, the feeding behaviors of the insects were evaluated on young leaves from citrus seedlings. These analyses proved that knockdown of D. citri effectors also interfered with ACP feeding abilities in planta, causing a decrease in honeydew production and reducing ACP survival. Electrical penetration graph (EPG) analysis confirmed the actions of the effectors on D. citri feeding behaviors. These results indicate that gene silencing of D. citri effectors may cause changes in D. citri feeding behaviors and could potentially be used for ACP control.
2017
Waculicz-Andrade, C. E.; Savi, D. C.; Bini, A. P.; Adamoski, D.; Goulin, E. H.; Silva, G. J.; Massola, N. S.; Terasawa, L. G.; Kava, V.; Glienke, Chirlei
Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil? Journal Article
In: Australasian Plant Pathology 2017 46:2, vol. 46, iss. 2, pp. 191-203, 2017, ISSN: 1448-6032.
Abstract | Links | BibTeX | Tags: agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences
@article{nokey,
title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},
author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},
url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},
doi = {10.1007/S13313-017-0476-1},
issn = {1448-6032},
year = {2017},
date = {2017-01-01},
journal = {Australasian Plant Pathology 2017 46:2},
volume = {46},
issue = {2},
pages = {191-203},
publisher = {Springer},
abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},
keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},
pubstate = {published},
tppubtype = {article}
}
Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.
Waculicz-Andrade, C. E.; Savi, D. C.; Bini, A. P.; Adamoski, D.; Goulin, E. H.; Silva, G. J.; Massola, N. S.; Terasawa, L. G.; Kava, V.; Glienke, Chirlei
Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil? Journal Article
In: Australasian Plant Pathology 2017 46:2, vol. 46, iss. 2, pp. 191-203, 2017, ISSN: 1448-6032.
Abstract | Links | BibTeX | Tags: agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences
@article{nokey,
title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},
author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},
url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},
doi = {10.1007/S13313-017-0476-1},
issn = {1448-6032},
year = {2017},
date = {2017-01-01},
journal = {Australasian Plant Pathology 2017 46:2},
volume = {46},
issue = {2},
pages = {191-203},
publisher = {Springer},
abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},
keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},
pubstate = {published},
tppubtype = {article}
}
Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.
Waculicz-Andrade, C. E.; Savi, D. C.; Bini, A. P.; Adamoski, D.; Goulin, E. H.; Silva, G. J.; Massola, N. S.; Terasawa, L. G.; Kava, V.; Glienke, Chirlei
Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil? Journal Article
In: Australasian Plant Pathology 2017 46:2, vol. 46, iss. 2, pp. 191-203, 2017, ISSN: 1448-6032.
Abstract | Links | BibTeX | Tags: agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences
@article{nokey,
title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},
author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},
url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},
doi = {10.1007/S13313-017-0476-1},
issn = {1448-6032},
year = {2017},
date = {2017-01-01},
journal = {Australasian Plant Pathology 2017 46:2},
volume = {46},
issue = {2},
pages = {191-203},
publisher = {Springer},
abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},
keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},
pubstate = {published},
tppubtype = {article}
}
Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.