Publications

@article{Vieira2018,

title = {Terrestrial and marine Antarctic fungi extracts active against Xanthomonas citri subsp. citri},

author = {G. Vieira and J. Purić and L. G. Morão and J. A. Santos and F. J. Inforsato and L. D. Sette and H. Ferreira and D. C. Sass},

url = {https://onlinelibrary.wiley.com/doi/full/10.1111/lam.12890 https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.12890 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/lam.12890},

doi = {10.1111/LAM.12890},

issn = {1472-765X},

year  = {2018},

date = {2018-01-01},

journal = {Letters in Applied Microbiology},

volume = {67},

issue = {1},

pages = {64-71},

publisher = {John Wiley & Sons, Ltd},

abstract = {This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctica and assess its potential antibacterial activity on Xanthomonas citri subsp. citri, the agent of citrus canker. Metabolites production was conducted in Malt 2% broth at 15°C for 20 days after which intracellular and extracellular extracts were obtained. The extracts were evaluated by cell viability assays through Resazurin Microtitre Assay. From 158 fungal extracts, 33 hampered bacterial growth in vitro. The average inhibition of the extracts obtained from terrestrial (soil) and marine (sediments) fungi was 94 and 97% respectively. These inhibition values were close to the average of 90% cell death for the positive control. MIC90 and MBC for the bioactive extracts were established. Isolates that produced active metabolites against the phytopathogen were identified using molecular taxonomy (ITS-rRNA sequencing) as: Pseudogymnoascus, Penicillium, Cadophora, Paraconiothyrium and Toxicocladosporium. Antarctic fungal strains isolated from terrestrial and marine sediments were able to produce secondary metabolites with antimicrobial activity against X. citri subsp. citri, highlighting the importance of these microbial genetic resources. These metabolites have potential to be used as alternatives for the control of this plant pathogen. Significance and Impact of the Study: This manuscript makes an impact on the study of micro-organisms from extreme habitats and their possible contribution in discovering new active molecules against pathogens of agricultural interest. Studies on the Antarctic continent and its communities have attracted the scientific community due to the long period of isolation and low levels of disturbance that surrounds the region. Knowing the potential of fungi in this region to produce active secondary metabolites, we aim to contribute to the discovery of compounds with antibacterial action in Xanthomonas citri subsp. citri, a plant pathogen present in several regions around the globe.},

keywords = {Antarctic fungi, antibacterial action, Citrus canker, Secondary metabolites, Xanthomonas},

pubstate = {published},

tppubtype = {article}

}

@article{Tonial2017,

title = {Biological activity of Diaporthe terebinthifolii extracts against Phyllosticta citricarpa},

author = {Fabiana Tonial and Beatriz H. L. N. S. Maia and Andrea M. Sobottka and Daiani C. Savi and Vânia A. Vicente and Renata R. Gomes and Chirlei Glienke},

url = {https://academic.oup.com/femsle/article/364/5/fnx026/2967563},

doi = {10.1093/FEMSLE/FNX026},

issn = {0378-1097},

year  = {2017},

date = {2017-01-01},

journal = {FEMS Microbiology Letters},

volume = {364},

issue = {5},

pages = {26},

publisher = {Oxford Academic},

abstract = {Citrus black spot disease, caused by the phytopathogen Phyllosticta citricarpa, depreciates the market value of citrus fruits and prevents their exportation to disease-free regions. It may also reduce the productivity of citrus fruit orchards. To identify an alternative to conventional disease control measures, isolates of Diaporthe terebinthifolii, active against P. citricarpa, were selected from an endophytic fungal population of Schinus terebinthifolia leaves. Different culture media were screened to identify the culture medium that afforded the most efficient production of biologically active extracts. A particular fraction (fraction VI) of the extract completely protected orange leaves by inhibiting the germination of P. citricarpa conidia with a minimum inhibitory concentration of 0.003 μg.mL-1. The active constituents in D. terebinthifolii extract fractions were identified by gas chromatography coupled to mass spectrometry as verbanol, phenylethyl alcohol, verbenyl acetate and methyl hexadecanoate. The results obtained strongly suggest the existence of a synergistic effect among the metabolites produced. Thus, these fungal metabolites could be used to control the CBS disease. As the asexual spores of P. citricarpa play an important role in fruit lesion development and disease dispersion, fungal extracts that inhibit the spore germination can be used as an effective alternative for directional disease control.},

keywords = {Aroeira, Citrus black spot, Endophyte, Fungi, Secondary metabolites, Synergism},

pubstate = {published},

tppubtype = {article}

}