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Check the list of papers from NScTI Citrus
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2019
Rodrigues, Carolina Munari; Takita, Marco Aurélio; Silva, Nicholas Vinicius; Ribeiro-Alves, Marcelo; MacHado, Marcos Antonio
Comparative genome analysis of Phyllosticta citricarpa and Phyllosticta capitalensis, two fungi species that share the same host Journal Article
In: BMC Genomics, vol. 20, iss. 1, pp. 1-12, 2019, ISSN: 14712164.
Abstract | Links | BibTeX | Tags: Citrus black spot, Endophytic species, Functional enrichment analysis, Gene family expansion, Pathogenic species, Phylogeny
@article{Rodrigues2019,
title = {Comparative genome analysis of Phyllosticta citricarpa and Phyllosticta capitalensis, two fungi species that share the same host},
author = {Carolina Munari Rodrigues and Marco Aurélio Takita and Nicholas Vinicius Silva and Marcelo Ribeiro-Alves and Marcos Antonio MacHado},
url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5911-y},
doi = {10.1186/S12864-019-5911-Y/TABLES/2},
issn = {14712164},
year = {2019},
date = {2019-01-01},
journal = {BMC Genomics},
volume = {20},
issue = {1},
pages = {1-12},
publisher = {BioMed Central Ltd.},
abstract = {Background: Citrus are among the most important crops in the world. However, there are many diseases that affect Citrus caused by different pathogens. Citrus also hosts many symbiotic microorganisms in a relationship that may be advantageous for both organisms. The fungi Phyllosticta citricarpa, responsible for citrus black spot, and Phyllosticta capitalensis, an endophytic species, are examples of closely related species with different behavior in citrus. Both species are always biologically associated and are morphologically very similar, and comparing their genomes could help understanding the different lifestyles. In this study, a comparison was carried to identify genetic differences that could help us to understand the biology of P. citricarpa and P. capitalensis. Results: Drafts genomes were assembled with sizes close to 33 Mb for both fungi, carrying 15,206 and 14,797 coding sequences for P. citricarpa and P. capitalensis, respectively. Even though the functional categories of these coding sequences is similar, enrichment analysis showed that the pathogenic species presents growth and development genes that may be necessary for the pathogenicity of P. citricarpa. On the other hand, family expansion analyses showed the plasticity of the genome of these species. Particular families are expanded in the genome of an ancestor of P. capitalensis and a recent expansion can also be detected among this species. Additionally, evolution could be driven by environmental cues in P. citricarpa. Conclusions: This work demonstrated genomic differences between P. citricarpa and P. capitalensis. Although the idea that these differences could explain the different lifestyles of these fungi, we were not able to confirm this hypothesis. Genome evolution seems to be of real importance among the Phyllosticta isolates and it is leading to different biological characteristics of these species.},
keywords = {Citrus black spot, Endophytic species, Functional enrichment analysis, Gene family expansion, Pathogenic species, Phylogeny},
pubstate = {published},
tppubtype = {article}
}
Background: Citrus are among the most important crops in the world. However, there are many diseases that affect Citrus caused by different pathogens. Citrus also hosts many symbiotic microorganisms in a relationship that may be advantageous for both organisms. The fungi Phyllosticta citricarpa, responsible for citrus black spot, and Phyllosticta capitalensis, an endophytic species, are examples of closely related species with different behavior in citrus. Both species are always biologically associated and are morphologically very similar, and comparing their genomes could help understanding the different lifestyles. In this study, a comparison was carried to identify genetic differences that could help us to understand the biology of P. citricarpa and P. capitalensis. Results: Drafts genomes were assembled with sizes close to 33 Mb for both fungi, carrying 15,206 and 14,797 coding sequences for P. citricarpa and P. capitalensis, respectively. Even though the functional categories of these coding sequences is similar, enrichment analysis showed that the pathogenic species presents growth and development genes that may be necessary for the pathogenicity of P. citricarpa. On the other hand, family expansion analyses showed the plasticity of the genome of these species. Particular families are expanded in the genome of an ancestor of P. capitalensis and a recent expansion can also be detected among this species. Additionally, evolution could be driven by environmental cues in P. citricarpa. Conclusions: This work demonstrated genomic differences between P. citricarpa and P. capitalensis. Although the idea that these differences could explain the different lifestyles of these fungi, we were not able to confirm this hypothesis. Genome evolution seems to be of real importance among the Phyllosticta isolates and it is leading to different biological characteristics of these species.
2017
Tonial, Fabiana; Maia, Beatriz H. L. N. S.; Sobottka, Andrea M.; Savi, Daiani C.; Vicente, Vânia A.; Gomes, Renata R.; Glienke, Chirlei
Biological activity of Diaporthe terebinthifolii extracts against Phyllosticta citricarpa Journal Article
In: FEMS Microbiology Letters, vol. 364, iss. 5, pp. 26, 2017, ISSN: 0378-1097.
Abstract | Links | BibTeX | Tags: Aroeira, Citrus black spot, Endophyte, Fungi, Secondary metabolites, Synergism
@article{Tonial2017,
title = {Biological activity of Diaporthe terebinthifolii extracts against Phyllosticta citricarpa},
author = {Fabiana Tonial and Beatriz H. L. N. S. Maia and Andrea M. Sobottka and Daiani C. Savi and Vânia A. Vicente and Renata R. Gomes and Chirlei Glienke},
url = {https://academic.oup.com/femsle/article/364/5/fnx026/2967563},
doi = {10.1093/FEMSLE/FNX026},
issn = {0378-1097},
year = {2017},
date = {2017-01-01},
journal = {FEMS Microbiology Letters},
volume = {364},
issue = {5},
pages = {26},
publisher = {Oxford Academic},
abstract = {Citrus black spot disease, caused by the phytopathogen Phyllosticta citricarpa, depreciates the market value of citrus fruits and prevents their exportation to disease-free regions. It may also reduce the productivity of citrus fruit orchards. To identify an alternative to conventional disease control measures, isolates of Diaporthe terebinthifolii, active against P. citricarpa, were selected from an endophytic fungal population of Schinus terebinthifolia leaves. Different culture media were screened to identify the culture medium that afforded the most efficient production of biologically active extracts. A particular fraction (fraction VI) of the extract completely protected orange leaves by inhibiting the germination of P. citricarpa conidia with a minimum inhibitory concentration of 0.003 μg.mL-1. The active constituents in D. terebinthifolii extract fractions were identified by gas chromatography coupled to mass spectrometry as verbanol, phenylethyl alcohol, verbenyl acetate and methyl hexadecanoate. The results obtained strongly suggest the existence of a synergistic effect among the metabolites produced. Thus, these fungal metabolites could be used to control the CBS disease. As the asexual spores of P. citricarpa play an important role in fruit lesion development and disease dispersion, fungal extracts that inhibit the spore germination can be used as an effective alternative for directional disease control.},
keywords = {Aroeira, Citrus black spot, Endophyte, Fungi, Secondary metabolites, Synergism},
pubstate = {published},
tppubtype = {article}
}
Citrus black spot disease, caused by the phytopathogen Phyllosticta citricarpa, depreciates the market value of citrus fruits and prevents their exportation to disease-free regions. It may also reduce the productivity of citrus fruit orchards. To identify an alternative to conventional disease control measures, isolates of Diaporthe terebinthifolii, active against P. citricarpa, were selected from an endophytic fungal population of Schinus terebinthifolia leaves. Different culture media were screened to identify the culture medium that afforded the most efficient production of biologically active extracts. A particular fraction (fraction VI) of the extract completely protected orange leaves by inhibiting the germination of P. citricarpa conidia with a minimum inhibitory concentration of 0.003 μg.mL-1. The active constituents in D. terebinthifolii extract fractions were identified by gas chromatography coupled to mass spectrometry as verbanol, phenylethyl alcohol, verbenyl acetate and methyl hexadecanoate. The results obtained strongly suggest the existence of a synergistic effect among the metabolites produced. Thus, these fungal metabolites could be used to control the CBS disease. As the asexual spores of P. citricarpa play an important role in fruit lesion development and disease dispersion, fungal extracts that inhibit the spore germination can be used as an effective alternative for directional disease control.