Publications

@article{Kishi2018,

title = {Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus},

author = {Rosangela Naomi Inui Kishi and Dagmar Stach-Machado and Junya Lacorte Singulani and Claudia Tavares Santos and Ana Marisa Fusco-Almeida and Eduardo Maffud Cilli and Juliana Freitas-Astúa and Simone Cristina Picchi and Marcos Antonio Machado},

url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203451},

doi = {10.1371/JOURNAL.PONE.0203451},

issn = {1932-6203},

year  = {2018},

date = {2018-01-01},

journal = {PLOS ONE},

volume = {13},

issue = {9},

pages = {e0203451},

publisher = {Public Library of Science},

abstract = {Antimicrobial peptides (AMPs) can be found in various organisms, and could be considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens.},

keywords = {Agrobacterium tumefaciens, Cell membranes, Citrus, Cytotoxicity, Leaves, Lipids, Toxicity, Vesicles},

pubstate = {published},

tppubtype = {article}

}

@article{Silva2017,

title = {Evaluation of cytotoxic, apoptotic, mutagenic, and chemopreventive activities of semi-synthetic esters of gallic acid},

author = {I. C. Silva and C. R. Polaquini and L. O. Regasini and H. Ferreira and F. R. Pavan},

doi = {10.1016/J.FCT.2017.04.033},

issn = {0278-6915},

year  = {2017},

date = {2017-01-01},

journal = {Food and Chemical Toxicology},

volume = {105},

pages = {300-307},

publisher = {Pergamon},

abstract = {Gallic acid and its derivatives are phenolic compounds widely used as food supplements in the form of capsules, liquid extracts, and ointments owing to their good antioxidant properties. Besides, these compounds are potent inhibitors of fungi, bacteria, and some viruses and possess strong antiproliferative and chemopreventive activities. However, gallic acid derivatives are also known to exert harmful effects like mutagenicity and cytotoxicity. The present study aimed to understand and explore the toxicological risks of these compounds. For this, a series of alkyl gallates with side chains varying from five to eight carbons (pentyl, hexyl, heptyl, and octyl gallates) were evaluated for their cytotoxic and pro-apoptotic potential. In addition, the genotoxic effects of alkyl gallates were measured in HepG2 cells using the single cell gel electrophoresis (SCGE)/comet assay and the cytokinesis-blocked micronucleus (CBMN) test. In both the tests, the substances did not induce any significant differences when compared to the control group. In addition, alkyl gallates exhibited a chemopreventive effect, thereby considerably reducing the mutagenicity caused by H2O2. In conclusion, our results suggest that alkyl gallates are non-genotoxic, non-mutagenic, and pro-apoptotic agents, which may serve as suitable and promising candidates for preventing chemically-induced chromosomal damage.},

keywords = {Alkyl gallates, Cell death, Chemoprotective, DNA damage, Micronucleus, Toxicity},

pubstate = {published},

tppubtype = {article}

}