Publicações

@article{Moreira2018,

title = {Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages in Bixa orellana L.},

author = {Viviane S. Moreira and Virgínia L. F. Soares and Raner J. S. Silva and Aurizangela O. Sousa and Wagner C. Otoni and Marcio G. C. Costa},

url = {https://link.springer.com/article/10.1007/s12298-018-0528-1},

doi = {10.1007/S12298-018-0528-1},

issn = {0974-0430},

year  = {2018},

date = {2018-01-01},

journal = {Physiology and Molecular Biology of Plants 2018 24:3},

volume = {24},

issue = {3},

pages = {369-378},

publisher = {Springer},

abstract = {Bixa orellana L., popularly known as annatto, produces several secondary metabolites of pharmaceutical and industrial interest, including bixin, whose molecular basis of biosynthesis remain to be determined. Gene expression analysis by quantitative real-time PCR (qPCR) is an important tool to advance such knowledge. However, correct interpretation of qPCR data requires the use of suitable reference genes in order to reduce experimental variations. In the present study, we have selected four different candidates for reference genes in B. orellana, coding for 40S ribosomal protein S9 (RPS9), histone H4 (H4), 60S ribosomal protein L38 (RPL38) and 18S ribosomal RNA (18SrRNA). Their expression stabilities in different tissues (e.g. flower buds, flowers, leaves and seeds at different developmental stages) were analyzed using five statistical tools (NormFinder, geNorm, BestKeeper, ΔCt method and RefFinder). The results indicated that RPL38 is the most stable gene in different tissues and stages of seed development and 18SrRNA is the most unstable among the analyzed genes. In order to validate the candidate reference genes, we have analyzed the relative expression of a target gene coding for carotenoid cleavage dioxygenase 1 (CCD1) using the stable RPL38 and the least stable gene, 18SrRNA, for normalization of the qPCR data. The results demonstrated significant differences in the interpretation of the CCD1 gene expression data, depending on the reference gene used, reinforcing the importance of the correct selection of reference genes for normalization.},

keywords = {Biological and Medical Physics, Biophysics, Cell Biology, Plant physiology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{Martins2017b,

title = {Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions},

author = {Cristina P. S. Martins and Diana M. Neves and Luciana C. Cidade and Amanda F. S. Mendes and Delmira C. Silva and Alex Alan F. Almeida and Mauricio A. Coelho-Filho and Abelmon S. Gesteira and Walter S. Soares-Filho and Marcio G. C. Costa},

url = {https://link.springer.com/article/10.1007/s00425-017-2653-4},

doi = {10.1007/S00425-017-2653-4},

issn = {1432-2048},

year  = {2017},

date = {2017-01-01},

journal = {Planta 2017 245:5},

volume = {245},

issue = {5},

pages = {951-963},

publisher = {Springer},

abstract = {Overexpression of the citrus CsTIP2;1 improves plant growth and tolerance to salt and drought stresses by enhancing cell expansion, H 2 O 2 detoxification and stomatal conductance. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing disease, suggesting its involvement in the regulation of the flow of water and nutrients required during both normal growth and stress conditions. Here, we show that the overexpression of CsTIP2;1 in transgenic tobacco increases plant growth under optimal and water- and salt-stress conditions and also significantly improves the leaf water and oxidative status, photosynthetic capacity, transpiration rate and water use efficiency of plants subjected to a progressive soil drying. These results correlated with the enhanced mesophyll cell expansion, midrib aquiferous parenchyma abundance, H2O2 detoxification and stomatal conductance observed in the transgenic plants. Taken together, our results indicate that CsTIP2;1 plays an active role in regulating the water and oxidative status required for plant growth and adaptation to stressful environmental conditions and may be potentially useful for engineering stress tolerance in citrus and other crop plants.},

keywords = {agriculture, Ecology, Forestry, Orange, photosynthesis, Plant Sciences, Reactive oxygen species},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},

author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},

doi = {10.1007/S13313-017-0476-1},

issn = {1448-6032},

year  = {2017},

date = {2017-01-01},

journal = {Australasian Plant Pathology 2017 46:2},

volume = {46},

issue = {2},

pages = {191-203},

publisher = {Springer},

abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},

keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},

author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},

doi = {10.1007/S13313-017-0476-1},

issn = {1448-6032},

year  = {2017},

date = {2017-01-01},

journal = {Australasian Plant Pathology 2017 46:2},

volume = {46},

issue = {2},

pages = {191-203},

publisher = {Springer},

abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},

keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{Cardoso2017,

title = {Somatic embryogenesis of a seedless sweet orange (Citrus sinensis (L.) Osbeck)},

author = {Jean C. Cardoso and Maisa Curtolo and Rodrigo R. Latado and Adriana P. Martinelli},

url = {https://link.springer.com/article/10.1007/s11627-017-9866-x},

doi = {10.1007/S11627-017-9866-X},

issn = {1475-2689},

year  = {2017},

date = {2017-01-01},

journal = {In Vitro Cellular & Developmental Biology - Plant 2017 53:6},

volume = {53},

issue = {6},

pages = {619-623},

publisher = {Springer},

abstract = {Somatic embryogenesis is an important in vitro technique used to obtain Citrus sinensis (L.) Osbeck (sweet orange) plantlets for conservation, sanitation, propagation, and breeding. The induction of somatic embryogenesis from adult tissues of sweet orange could be an alternative to in vitro organogenesis from epicotyl segments, especially in seedless cultivars, where the latter is not feasible. The aim of this study was to obtain plantlets from ovary-derived somatic embryos of sweet orange cv. ‘Washington Navel’, an important seedless cultivar for citrus fresh fruit production. The explants used were pistils from flower buds, pre-anthesis, from 20-y-old plants cultivated in the field. Forty plantlets from 47 somatic embryos were obtained, in vitro-grafted, and acclimatized in greenhouse conditions. Ploidy evaluation through flow cytometric analysis, as well as the results of target region amplification polymorphism (TRAP) molecular markers confirmed the somatic origin of embryos as genetically similar to donor plants. This technique could be used for obtaining embryogenic cell suspension cultures or regenerated plants from mature tissues other than seed-derived tissues, especially for seedless genotypes.},

keywords = {Cell Biology, Developmental Biology, Plant Breeding/Biotechnology, Plant Genetics and Genomics, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{Martins2017,

title = {Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions},

author = {Cristina P. S. Martins and Diana M. Neves and Luciana C. Cidade and Amanda F. S. Mendes and Delmira C. Silva and Alex Alan F. Almeida and Mauricio A. Coelho-Filho and Abelmon S. Gesteira and Walter S. Soares-Filho and Marcio G. C. Costa},

url = {https://link.springer.com/article/10.1007/s00425-017-2653-4},

doi = {10.1007/S00425-017-2653-4},

issn = {1432-2048},

year  = {2017},

date = {2017-01-01},

journal = {Planta 2017 245:5},

volume = {245},

issue = {5},

pages = {951-963},

publisher = {Springer},

abstract = {Overexpression of the citrus CsTIP2;1 improves plant growth and tolerance to salt and drought stresses by enhancing cell expansion, H 2 O 2 detoxification and stomatal conductance. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing disease, suggesting its involvement in the regulation of the flow of water and nutrients required during both normal growth and stress conditions. Here, we show that the overexpression of CsTIP2;1 in transgenic tobacco increases plant growth under optimal and water- and salt-stress conditions and also significantly improves the leaf water and oxidative status, photosynthetic capacity, transpiration rate and water use efficiency of plants subjected to a progressive soil drying. These results correlated with the enhanced mesophyll cell expansion, midrib aquiferous parenchyma abundance, H2O2 detoxification and stomatal conductance observed in the transgenic plants. Taken together, our results indicate that CsTIP2;1 plays an active role in regulating the water and oxidative status required for plant growth and adaptation to stressful environmental conditions and may be potentially useful for engineering stress tolerance in citrus and other crop plants.},

keywords = {agriculture, Ecology, Forestry, Orange, photosynthesis, Plant Sciences, Reactive oxygen species},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},

author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},

doi = {10.1007/S13313-017-0476-1},

issn = {1448-6032},

year  = {2017},

date = {2017-01-01},

journal = {Australasian Plant Pathology 2017 46:2},

volume = {46},

issue = {2},

pages = {191-203},

publisher = {Springer},

abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},

keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},

pubstate = {published},

tppubtype = {article}

}

@article{Zambrosi2017,

title = {Anatomical and ultrastructural damage to citrus leaves from phosphite spray depends on phosphorus supply to roots},

author = {Fernando C. B. Zambrosi and Geisa L. Mesquita and Gillyade Menino and Francisco A. O. Tanaka and Dirceu Mattos and Jose A. Quaggio},

url = {https://link.springer.com/article/10.1007/s11104-017-3314-x},

doi = {10.1007/S11104-017-3314-X},

issn = {1573-5036},

year  = {2017},

date = {2017-01-01},

journal = {Plant and Soil 2017 418:1},

volume = {418},

issue = {1},

pages = {557-569},

publisher = {Springer},

abstract = {Although phosphite (PO3, Phi) cannot replace phosphate (PO4, Pi) as a nutritional source of phosphorus (P) for plants, decisions about using foliar Phi application in citrus groves scarcely take into account P availability and plant nutritional status. Accordingly, we studied the interactive effects of P supply in the rooting medium and foliar sprays of Pi or Phi on citrus tree performance and leaf anatomy and ultrastructure. Young sweet orange trees were grown hydroponically using P-deficient (PD) or P-sufficient (PS) nutrient solutions for 15 weeks in combination with 3 foliar spray treatments: 0.16 M P in the form of Pi (KH2PO4) or Phi (KH2PO3) and a control spray of 0.16 M potassium chloride (KCl). Six foliar sprays were applied to the drip point at 15-day intervals during the experimental period. Before the trees were harvested to estimate growth, mature leaves were sampled to study the anatomical and ultrastructural organization and to evaluate P concentration. Under PD, the Pi spray stimulated tree growth compared with the control, whereas Phi sprays impaired tree performance, suggesting toxic effects. Both Pi and Phi applications increased the leaf P concentration relative to that of the control. Leaves of PD trees exposed to Phi exhibited pronounced damage to the epidermis, and stomata showed deformed ostioles and surface flaking. Moreover, phloem and xylem vessels were disorganized, cell wall presented sinuosity, cell membranes were plasmolyzed, and chloroplast thylakoids were disrupted, with accumulation of starch and plastoglobuli. However, no similar changes were observed either in PD trees under Pi spray or in PS trees independent of foliar treatment. PD trees receiving KCl exhibited intermediate responses between those of Pi and Phi applications. This was the first study to demonstrate that Phi spray disrupts the anatomical and ultrastructural organization of citrus leaves under P deficiency. Our results provide insights into the mechanisms explaining the poor nutritional value of Phi and support information to guide the use of Phi-based products.},

keywords = {Ecology, Plant physiology, Plant Sciences, Soil Science & Conservation, Starch, Stomata},

pubstate = {published},

tppubtype = {article}

}