Nossos Resultados
nas principais revistas científicas
do mundo.
Confira a lista de publicações do INCT CITROS
Utilize os filtros no topo da lista para selecionar artigos por ano ou por pesquisador(a). Na caixa de cada artigo, utilize os links ("Resumo", "Links", "BibTeX") para abrir informações adicionais.
PublicaçõesPesquisadores
Você pode utilizar a nuvem de tags abaixo para selecionar artigos por assunto.
2020
Kupper, Katia Cristina; Moretto, Rafael Kupper; Fujimoto, Andreia
Production of antifungal compounds by Bacillus spp. isolates and its capacity for controlling citrus black spot under field conditions Journal Article
Em: World Journal of Microbiology and Biotechnology, vol. 36, iss. 1, pp. 1-10, 2020, ISSN: 15730972.
Resumo | Links | BibTeX | Tags: Cell-free antifungal compounds, Citrus sinensis, Liquid chromatography, mass spectrometry, Phyllosticta citricarpa, Thermostable, Volatile
@article{Kupper2020,
title = {Production of antifungal compounds by Bacillus spp. isolates and its capacity for controlling citrus black spot under field conditions},
author = {Katia Cristina Kupper and Rafael Kupper Moretto and Andreia Fujimoto},
url = {https://link.springer.com/article/10.1007/s11274-019-2772-0},
doi = {10.1007/S11274-019-2772-0/METRICS},
issn = {15730972},
year = {2020},
date = {2020-01-01},
journal = {World Journal of Microbiology and Biotechnology},
volume = {36},
issue = {1},
pages = {1-10},
publisher = {Springer},
abstract = {Orange juice is a major agricultural product, and oranges are among the most widely sold fresh fruits in several countries. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, affects almost every species of citrus, causing a esthetic depreciation of the fruit and fruit drop, with consequent financial loss for its in natura commercialization. Fungicides are the major control measure for CBS, but have limited efficiency and high cost, and give rise to fungal strains resistant to these products. This work assayed the production of antifungal compounds by Bacillus spp. isolates and tested the potential of two isolates from B. subtilis (ACB-AP3 and ACB-83) for controlling CBS under field conditions with two previously untested orange varieties. A total of 15 isolates produced cell-free, thermostable or volatile compounds effective in suppressing P. citricarpa growth in vitro. It was possible to detect the production of two antibiotics (iturin and surfactin) by B. subtilis ACB-83. Additionally, B. subtilis isolates ACB-AP3 and ACB-83 displayed significant effects in controlling the disease under field conditions.},
keywords = {Cell-free antifungal compounds, Citrus sinensis, Liquid chromatography, mass spectrometry, Phyllosticta citricarpa, Thermostable, Volatile},
pubstate = {published},
tppubtype = {article}
}
Orange juice is a major agricultural product, and oranges are among the most widely sold fresh fruits in several countries. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, affects almost every species of citrus, causing a esthetic depreciation of the fruit and fruit drop, with consequent financial loss for its in natura commercialization. Fungicides are the major control measure for CBS, but have limited efficiency and high cost, and give rise to fungal strains resistant to these products. This work assayed the production of antifungal compounds by Bacillus spp. isolates and tested the potential of two isolates from B. subtilis (ACB-AP3 and ACB-83) for controlling CBS under field conditions with two previously untested orange varieties. A total of 15 isolates produced cell-free, thermostable or volatile compounds effective in suppressing P. citricarpa growth in vitro. It was possible to detect the production of two antibiotics (iturin and surfactin) by B. subtilis ACB-83. Additionally, B. subtilis isolates ACB-AP3 and ACB-83 displayed significant effects in controlling the disease under field conditions.
2019
Dória, Milena Santos; Guedes, Meg Silva; Silva, Edson Mario Andrade; Oliveira, Tahise Magalhães; Pirovani, Carlos Priminho; Kupper, Katia Cristina; Bastianel, Marinês; Micheli, Fabienne
Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata Journal Article
Em: International Journal of Biological Macromolecules, vol. 136, pp. 410-423, 2019, ISSN: 0141-8130.
Resumo | Links | BibTeX | Tags: ‘Clemenules’, ‘Minneola’, mass spectrometry
@article{nokey,
title = {Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata},
author = {Milena Santos Dória and Meg Silva Guedes and Edson Mario Andrade Silva and Tahise Magalhães Oliveira and Carlos Priminho Pirovani and Katia Cristina Kupper and Marinês Bastianel and Fabienne Micheli},
doi = {10.1016/J.IJBIOMAC.2019.06.069},
issn = {0141-8130},
year = {2019},
date = {2019-01-01},
journal = {International Journal of Biological Macromolecules},
volume = {136},
pages = {410-423},
publisher = {Elsevier},
abstract = {Alternaria brown spot (ABS) is a disease caused by the necrotrophic fungus Alternaria alternata, which induces necrotic lesions on fruits and young leaves due to the production of the host-specific ACT toxin by the fungus. To better understand the citrus–A. alternata interaction and to identify putative resistance proteins, as well as the receptor of the ACT toxin, citrus plants susceptible (‘Minneola’ mandarin) and resistant (‘Clemenules’ tangor) to A. alternata, infected or not (control) with the pathogen were analyzed by proteomics. Protein changes were observed between citrus genotypes after infection, and 150 candidate proteins were obtained. A general scheme of the metabolic processes involved in susceptible and resistant citrus–A. alternata interactions was designed. Susceptible plants presented a high level of proteins involved in stress response at the final stages of the infection, whereas resistant plants presented high level of ROS proteins, metabolic proteins, and proteins involved in the immune system process. Proteins like ferredoxin and cyclophilin are specific to the susceptible variety and may be good candidates as fungal effector-interacting proteins. This is the first citrus–A. alternata proteomics analysis, which has allowed a better understanding of the molecular bases of the citrus response to ABS disease.},
keywords = {‘Clemenules’, ‘Minneola’, mass spectrometry},
pubstate = {published},
tppubtype = {article}
}
Alternaria brown spot (ABS) is a disease caused by the necrotrophic fungus Alternaria alternata, which induces necrotic lesions on fruits and young leaves due to the production of the host-specific ACT toxin by the fungus. To better understand the citrus–A. alternata interaction and to identify putative resistance proteins, as well as the receptor of the ACT toxin, citrus plants susceptible (‘Minneola’ mandarin) and resistant (‘Clemenules’ tangor) to A. alternata, infected or not (control) with the pathogen were analyzed by proteomics. Protein changes were observed between citrus genotypes after infection, and 150 candidate proteins were obtained. A general scheme of the metabolic processes involved in susceptible and resistant citrus–A. alternata interactions was designed. Susceptible plants presented a high level of proteins involved in stress response at the final stages of the infection, whereas resistant plants presented high level of ROS proteins, metabolic proteins, and proteins involved in the immune system process. Proteins like ferredoxin and cyclophilin are specific to the susceptible variety and may be good candidates as fungal effector-interacting proteins. This is the first citrus–A. alternata proteomics analysis, which has allowed a better understanding of the molecular bases of the citrus response to ABS disease.
Dória, Milena Santos; Guedes, Meg Silva; Silva, Edson Mario Andrade; Oliveira, Tahise Magalhães; Pirovani, Carlos Priminho; Kupper, Katia Cristina; Bastianel, Marinês; Micheli, Fabienne
Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata Journal Article
Em: International Journal of Biological Macromolecules, vol. 136, pp. 410-423, 2019, ISSN: 0141-8130.
Resumo | Links | BibTeX | Tags: ‘Clemenules’, ‘Minneola’, mass spectrometry
@article{nokey,
title = {Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata},
author = {Milena Santos Dória and Meg Silva Guedes and Edson Mario Andrade Silva and Tahise Magalhães Oliveira and Carlos Priminho Pirovani and Katia Cristina Kupper and Marinês Bastianel and Fabienne Micheli},
doi = {10.1016/J.IJBIOMAC.2019.06.069},
issn = {0141-8130},
year = {2019},
date = {2019-01-01},
journal = {International Journal of Biological Macromolecules},
volume = {136},
pages = {410-423},
publisher = {Elsevier},
abstract = {Alternaria brown spot (ABS) is a disease caused by the necrotrophic fungus Alternaria alternata, which induces necrotic lesions on fruits and young leaves due to the production of the host-specific ACT toxin by the fungus. To better understand the citrus–A. alternata interaction and to identify putative resistance proteins, as well as the receptor of the ACT toxin, citrus plants susceptible (‘Minneola’ mandarin) and resistant (‘Clemenules’ tangor) to A. alternata, infected or not (control) with the pathogen were analyzed by proteomics. Protein changes were observed between citrus genotypes after infection, and 150 candidate proteins were obtained. A general scheme of the metabolic processes involved in susceptible and resistant citrus–A. alternata interactions was designed. Susceptible plants presented a high level of proteins involved in stress response at the final stages of the infection, whereas resistant plants presented high level of ROS proteins, metabolic proteins, and proteins involved in the immune system process. Proteins like ferredoxin and cyclophilin are specific to the susceptible variety and may be good candidates as fungal effector-interacting proteins. This is the first citrus–A. alternata proteomics analysis, which has allowed a better understanding of the molecular bases of the citrus response to ABS disease.},
keywords = {‘Clemenules’, ‘Minneola’, mass spectrometry},
pubstate = {published},
tppubtype = {article}
}
Alternaria brown spot (ABS) is a disease caused by the necrotrophic fungus Alternaria alternata, which induces necrotic lesions on fruits and young leaves due to the production of the host-specific ACT toxin by the fungus. To better understand the citrus–A. alternata interaction and to identify putative resistance proteins, as well as the receptor of the ACT toxin, citrus plants susceptible (‘Minneola’ mandarin) and resistant (‘Clemenules’ tangor) to A. alternata, infected or not (control) with the pathogen were analyzed by proteomics. Protein changes were observed between citrus genotypes after infection, and 150 candidate proteins were obtained. A general scheme of the metabolic processes involved in susceptible and resistant citrus–A. alternata interactions was designed. Susceptible plants presented a high level of proteins involved in stress response at the final stages of the infection, whereas resistant plants presented high level of ROS proteins, metabolic proteins, and proteins involved in the immune system process. Proteins like ferredoxin and cyclophilin are specific to the susceptible variety and may be good candidates as fungal effector-interacting proteins. This is the first citrus–A. alternata proteomics analysis, which has allowed a better understanding of the molecular bases of the citrus response to ABS disease.
2017
Silva, Michelli Massaroli Da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima Fernandes Da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves De
Em: Molecules 2017, Vol. 22, Page 985, vol. 22, iss. 6, pp. 985, 2017, ISSN: 1420-3049.
Resumo | Links | BibTeX | Tags: diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa
@article{nokey,
title = {A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry},
author = {Michelli Massaroli Da Silva and Moacir Dos Santos Andrade and Anelize Bauermeister and Marcus Vinícius Merfa and Moacir Rossi Forim and João Batista Fernandes and Paulo Cezar Vieira and Maria Fátima Fernandes Da Silva and Norberto Peporine Lopes and Marcos Antônio Machado and Alessandra Alves De Souza},
url = {https://www.mdpi.com/1420-3049/22/6/985/htm https://www.mdpi.com/1420-3049/22/6/985},
doi = {10.3390/MOLECULES22060985},
issn = {1420-3049},
year = {2017},
date = {2017-01-01},
journal = {Molecules 2017, Vol. 22, Page 985},
volume = {22},
issue = {6},
pages = {985},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.},
keywords = {diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.
Silva, Michelli Massaroli Da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima Fernandes Da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves De
Em: Molecules 2017, Vol. 22, Page 985, vol. 22, iss. 6, pp. 985, 2017, ISSN: 1420-3049.
Resumo | Links | BibTeX | Tags: diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa
@article{nokey,
title = {A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry},
author = {Michelli Massaroli Da Silva and Moacir Dos Santos Andrade and Anelize Bauermeister and Marcus Vinícius Merfa and Moacir Rossi Forim and João Batista Fernandes and Paulo Cezar Vieira and Maria Fátima Fernandes Da Silva and Norberto Peporine Lopes and Marcos Antônio Machado and Alessandra Alves De Souza},
url = {https://www.mdpi.com/1420-3049/22/6/985/htm https://www.mdpi.com/1420-3049/22/6/985},
doi = {10.3390/MOLECULES22060985},
issn = {1420-3049},
year = {2017},
date = {2017-01-01},
journal = {Molecules 2017, Vol. 22, Page 985},
volume = {22},
issue = {6},
pages = {985},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.},
keywords = {diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.
Silva, Michelli Massaroli Da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima Fernandes Da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves De
Em: Molecules 2017, Vol. 22, Page 985, vol. 22, iss. 6, pp. 985, 2017, ISSN: 1420-3049.
Resumo | Links | BibTeX | Tags: diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa
@article{nokey,
title = {A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry},
author = {Michelli Massaroli Da Silva and Moacir Dos Santos Andrade and Anelize Bauermeister and Marcus Vinícius Merfa and Moacir Rossi Forim and João Batista Fernandes and Paulo Cezar Vieira and Maria Fátima Fernandes Da Silva and Norberto Peporine Lopes and Marcos Antônio Machado and Alessandra Alves De Souza},
url = {https://www.mdpi.com/1420-3049/22/6/985/htm https://www.mdpi.com/1420-3049/22/6/985},
doi = {10.3390/MOLECULES22060985},
issn = {1420-3049},
year = {2017},
date = {2017-01-01},
journal = {Molecules 2017, Vol. 22, Page 985},
volume = {22},
issue = {6},
pages = {985},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.},
keywords = {diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.