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2021
Silva, Danielle Fernandes; Amaral, Jéssica Cristina; Carlos, Rose Maria; Ferreira, Antonio Gilberto; Forim, Moacir Rossi; Fernandes, João Batista; Silva, Maria Fátima; Filho, Helvécio Della Coletta; Souza, Alessandra Alves
Em: Talanta, vol. 225, pp. 122040, 2021, ISSN: 0039-9140.
Resumo | Links | BibTeX | Tags: Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa
@article{nokey,
title = {Octahedral ruthenium and magnesium naringenin 5-alkoxide complexes: NMR analysis of diastereoisomers and in-vivo antibacterial activity against Xylella fastidiosa},
author = {Danielle Fernandes Silva and Jéssica Cristina Amaral and Rose Maria Carlos and Antonio Gilberto Ferreira and Moacir Rossi Forim and João Batista Fernandes and Maria Fátima Silva and Helvécio Della Coletta Filho and Alessandra Alves Souza},
doi = {10.1016/J.TALANTA.2020.122040},
issn = {0039-9140},
year = {2021},
date = {2021-01-01},
journal = {Talanta},
volume = {225},
pages = {122040},
publisher = {Elsevier},
abstract = {Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.},
keywords = {Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.
Silva, Danielle Fernandes; Amaral, Jéssica Cristina; Carlos, Rose Maria; Ferreira, Antonio Gilberto; Forim, Moacir Rossi; Fernandes, João Batista; Silva, Maria Fátima; Filho, Helvécio Della Coletta; Souza, Alessandra Alves
Em: Talanta, vol. 225, pp. 122040, 2021, ISSN: 0039-9140.
Resumo | Links | BibTeX | Tags: Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa
@article{nokey,
title = {Octahedral ruthenium and magnesium naringenin 5-alkoxide complexes: NMR analysis of diastereoisomers and in-vivo antibacterial activity against Xylella fastidiosa},
author = {Danielle Fernandes Silva and Jéssica Cristina Amaral and Rose Maria Carlos and Antonio Gilberto Ferreira and Moacir Rossi Forim and João Batista Fernandes and Maria Fátima Silva and Helvécio Della Coletta Filho and Alessandra Alves Souza},
doi = {10.1016/J.TALANTA.2020.122040},
issn = {0039-9140},
year = {2021},
date = {2021-01-01},
journal = {Talanta},
volume = {225},
pages = {122040},
publisher = {Elsevier},
abstract = {Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.},
keywords = {Bactericides, Citrus, Nuclear magnetic resonance, Real-time quantitative PCR, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Although many copper-based antimicrobial compounds have been developed to control pathogenic bacteria and fungi in plants and applied for crop protection, there is evidence that several plant pathogens have developed resistance to copper-based antimicrobial compounds, including some Xanthomonas species. Xylella is a bacterial genus belonging to the Xanthomonas family; and X. fastidiosa, which is responsible for citrus variegated chlorosis (CVC) in sweet orange, may develop resistance to one or more copper-based antimicrobials. Because of the time required for the development and approval of new antimicrobials for commercial use, the discovery of novel bactericidal compounds is essential before the development of resistance to the antimicrobials currently in use becomes widespread. Here, we explored the antimicrobial potential of two newly synthesized antimicrobials complexes and one natural compound against X. fastidiosa. Several nuclear magnetic resonance (NMR) assays with high resolution and sensitivity were developed to identify new diastereoisomers in the context of octahedral ruthenium - [Ru(narin)(phen)2]PF6-and magnesium naringenin 5-alkoxide - [Mg(narin)(phen)2]OAc - complexes, obtained in the present work. The NMR assays proved to be powerful tools for the identification of isomers in metal complexes. Moreover, a protocol for the in-vivo determination of the effects of these complexes against X. fastidiosa was developed. The main trunks of X. fastidiosa infected plants were injected with the two complexes as well as with the limonoid azadirachtin using a syringe; the number of bacterial cells in the plants following treatment was estimated via real-time quantitative PCR (qPCR). Importantly, the administration of both complexes and of azadirachtin drastically reduced the number of X. fastidiosa cells in vivo.
2020
Coletta-Filho, Helvecio Della; Castillo, Andreina I.; Laranjeira, Francisco Ferraz; Andrade, Eduardo Chumbinho; Silva, Natalia Teixeira; Souza, Alessandra Alves; Bossi, Mariana Esteves; Almeida, Rodrigo P. P.; Lopes, João R. S.
Citrus Variegated Chlorosis: an Overview of 30 Years of Research and Disease Management Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 3, pp. 175-191, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Citrus bacterium disease, Citrus sinensis, Xylella fastidiosa
@article{nokey,
title = {Citrus Variegated Chlorosis: an Overview of 30 Years of Research and Disease Management},
author = {Helvecio Della Coletta-Filho and Andreina I. Castillo and Francisco Ferraz Laranjeira and Eduardo Chumbinho Andrade and Natalia Teixeira Silva and Alessandra Alves Souza and Mariana Esteves Bossi and Rodrigo P. P. Almeida and João R. S. Lopes},
url = {https://link.springer.com/article/10.1007/s40858-020-00358-5},
doi = {10.1007/S40858-020-00358-5/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {3},
pages = {175-191},
publisher = {Springer},
abstract = {The emergence of citrus variegated chlorosis (CVC) disease had dramatic consequences to the citrus industry in Brazil. First reported in São Paulo State in 1987, this disease affected approximately 100 million sweet orange trees in the region 20 years later. However, current estimates indicate that the number of diseased trees has been reduced 25-fold since 2009. In this review we summarize research on CVC since its emergence, focusing on work that has contributed to the observed success in managing this disease in the field. Knowledge that CVC is caused by a bacterium (Xylella fastidiosa - now classified as X. fastidiosa subsp. pauca) that is transmited by infected plant material (grafting and nursery plant) and insect vectors, the citrus nursery production system switched in 2003 to a certification program in which plants are grown in insect proof screen-houses and routinely monitored for X. fastidiosa infection. Research triggered by the genome sequencing of a CVC isolate in 2000, the first plant pathogenic bacterium to have its complete genome sequenced, integrated molecular tools and approaches into research aimed at understanding the biology of this pathogen. Ultimately, the challenges imposed by CVC led to significant improvements in the scientific and technical knowledge linked to sweet orange production, and to the development of a more sustainable and resilient citrus industry in Brazil.},
keywords = {Citrus bacterium disease, Citrus sinensis, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The emergence of citrus variegated chlorosis (CVC) disease had dramatic consequences to the citrus industry in Brazil. First reported in São Paulo State in 1987, this disease affected approximately 100 million sweet orange trees in the region 20 years later. However, current estimates indicate that the number of diseased trees has been reduced 25-fold since 2009. In this review we summarize research on CVC since its emergence, focusing on work that has contributed to the observed success in managing this disease in the field. Knowledge that CVC is caused by a bacterium (Xylella fastidiosa - now classified as X. fastidiosa subsp. pauca) that is transmited by infected plant material (grafting and nursery plant) and insect vectors, the citrus nursery production system switched in 2003 to a certification program in which plants are grown in insect proof screen-houses and routinely monitored for X. fastidiosa infection. Research triggered by the genome sequencing of a CVC isolate in 2000, the first plant pathogenic bacterium to have its complete genome sequenced, integrated molecular tools and approaches into research aimed at understanding the biology of this pathogen. Ultimately, the challenges imposed by CVC led to significant improvements in the scientific and technical knowledge linked to sweet orange production, and to the development of a more sustainable and resilient citrus industry in Brazil.
Castillo, Andreina I.; Chacón-Díaz, Carlos; Rodríguez-Murillo, Neysa; Coletta-Filho, Helvecio D.; Almeida, Rodrigo P. P.
Impacts of local population history and ecology on the evolution of a globally dispersed pathogen Journal Article
Em: BMC Genomics, vol. 21, iss. 1, pp. 1-20, 2020, ISSN: 14712164.
Resumo | Links | BibTeX | Tags: genetic diversity, Inter-subspecific recombination, Pan genome, WGS, Xylella fastidiosa
@article{Castillo2020,
title = {Impacts of local population history and ecology on the evolution of a globally dispersed pathogen},
author = {Andreina I. Castillo and Carlos Chacón-Díaz and Neysa Rodríguez-Murillo and Helvecio D. Coletta-Filho and Rodrigo P. P. Almeida},
url = {https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-06778-6},
doi = {10.1186/S12864-020-06778-6/FIGURES/4},
issn = {14712164},
year = {2020},
date = {2020-01-01},
journal = {BMC Genomics},
volume = {21},
issue = {1},
pages = {1-20},
publisher = {BioMed Central Ltd.},
abstract = {Background: Pathogens with a global distribution face diverse biotic and abiotic conditions across populations. Moreover, the ecological and evolutionary history of each population is unique. Xylella fastidiosa is a xylem-dwelling bacterium infecting multiple plant hosts, often with detrimental effects. As a group, X. fastidiosa is divided into distinct subspecies with allopatric historical distributions and patterns of multiple introductions from numerous source populations. The capacity of X. fastidiosa to successfully colonize and cause disease in naïve plant hosts varies among subspecies, and potentially, among populations. Within Central America (i.e. Costa Rica) two X. fastidiosa subspecies coexist: the native subsp. fastidiosa and the introduced subsp. pauca. Using whole genome sequences, the patterns of gene gain/loss, genomic introgression, and genetic diversity were characterized within Costa Rica and contrasted to other X. fastidiosa populations. Results: Within Costa Rica, accessory and core genome analyses showed a highly malleable genome with numerous intra- and inter-subspecific gain/loss events. Likewise, variable levels of inter-subspecific introgression were found within and between both coexisting subspecies; nonetheless, the direction of donor/recipient subspecies to the recombinant segments varied. Some strains appeared to recombine more frequently than others; however, no group of genes or gene functions were overrepresented within recombinant segments. Finally, the patterns of genetic diversity of subsp. fastidiosa in Costa Rica were consistent with those of other native populations (i.e. subsp. pauca in Brazil). Conclusions: Overall, this study shows the importance of characterizing local evolutionary and ecological history in the context of world-wide pathogen distribution.},
keywords = {genetic diversity, Inter-subspecific recombination, Pan genome, WGS, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Background: Pathogens with a global distribution face diverse biotic and abiotic conditions across populations. Moreover, the ecological and evolutionary history of each population is unique. Xylella fastidiosa is a xylem-dwelling bacterium infecting multiple plant hosts, often with detrimental effects. As a group, X. fastidiosa is divided into distinct subspecies with allopatric historical distributions and patterns of multiple introductions from numerous source populations. The capacity of X. fastidiosa to successfully colonize and cause disease in naïve plant hosts varies among subspecies, and potentially, among populations. Within Central America (i.e. Costa Rica) two X. fastidiosa subspecies coexist: the native subsp. fastidiosa and the introduced subsp. pauca. Using whole genome sequences, the patterns of gene gain/loss, genomic introgression, and genetic diversity were characterized within Costa Rica and contrasted to other X. fastidiosa populations. Results: Within Costa Rica, accessory and core genome analyses showed a highly malleable genome with numerous intra- and inter-subspecific gain/loss events. Likewise, variable levels of inter-subspecific introgression were found within and between both coexisting subspecies; nonetheless, the direction of donor/recipient subspecies to the recombinant segments varied. Some strains appeared to recombine more frequently than others; however, no group of genes or gene functions were overrepresented within recombinant segments. Finally, the patterns of genetic diversity of subsp. fastidiosa in Costa Rica were consistent with those of other native populations (i.e. subsp. pauca in Brazil). Conclusions: Overall, this study shows the importance of characterizing local evolutionary and ecological history in the context of world-wide pathogen distribution.
Coletta-Filho, Helvecio Della; Castillo, Andreina I.; Laranjeira, Francisco Ferraz; Andrade, Eduardo Chumbinho; Silva, Natalia Teixeira; Souza, Alessandra Alves; Bossi, Mariana Esteves; Almeida, Rodrigo P. P.; Lopes, João R. S.
Citrus Variegated Chlorosis: an Overview of 30 Years of Research and Disease Management Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 3, pp. 175-191, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Citrus bacterium disease, Citrus sinensis, Xylella fastidiosa
@article{nokey,
title = {Citrus Variegated Chlorosis: an Overview of 30 Years of Research and Disease Management},
author = {Helvecio Della Coletta-Filho and Andreina I. Castillo and Francisco Ferraz Laranjeira and Eduardo Chumbinho Andrade and Natalia Teixeira Silva and Alessandra Alves Souza and Mariana Esteves Bossi and Rodrigo P. P. Almeida and João R. S. Lopes},
url = {https://link.springer.com/article/10.1007/s40858-020-00358-5},
doi = {10.1007/S40858-020-00358-5/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {3},
pages = {175-191},
publisher = {Springer},
abstract = {The emergence of citrus variegated chlorosis (CVC) disease had dramatic consequences to the citrus industry in Brazil. First reported in São Paulo State in 1987, this disease affected approximately 100 million sweet orange trees in the region 20 years later. However, current estimates indicate that the number of diseased trees has been reduced 25-fold since 2009. In this review we summarize research on CVC since its emergence, focusing on work that has contributed to the observed success in managing this disease in the field. Knowledge that CVC is caused by a bacterium (Xylella fastidiosa - now classified as X. fastidiosa subsp. pauca) that is transmited by infected plant material (grafting and nursery plant) and insect vectors, the citrus nursery production system switched in 2003 to a certification program in which plants are grown in insect proof screen-houses and routinely monitored for X. fastidiosa infection. Research triggered by the genome sequencing of a CVC isolate in 2000, the first plant pathogenic bacterium to have its complete genome sequenced, integrated molecular tools and approaches into research aimed at understanding the biology of this pathogen. Ultimately, the challenges imposed by CVC led to significant improvements in the scientific and technical knowledge linked to sweet orange production, and to the development of a more sustainable and resilient citrus industry in Brazil.},
keywords = {Citrus bacterium disease, Citrus sinensis, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The emergence of citrus variegated chlorosis (CVC) disease had dramatic consequences to the citrus industry in Brazil. First reported in São Paulo State in 1987, this disease affected approximately 100 million sweet orange trees in the region 20 years later. However, current estimates indicate that the number of diseased trees has been reduced 25-fold since 2009. In this review we summarize research on CVC since its emergence, focusing on work that has contributed to the observed success in managing this disease in the field. Knowledge that CVC is caused by a bacterium (Xylella fastidiosa - now classified as X. fastidiosa subsp. pauca) that is transmited by infected plant material (grafting and nursery plant) and insect vectors, the citrus nursery production system switched in 2003 to a certification program in which plants are grown in insect proof screen-houses and routinely monitored for X. fastidiosa infection. Research triggered by the genome sequencing of a CVC isolate in 2000, the first plant pathogenic bacterium to have its complete genome sequenced, integrated molecular tools and approaches into research aimed at understanding the biology of this pathogen. Ultimately, the challenges imposed by CVC led to significant improvements in the scientific and technical knowledge linked to sweet orange production, and to the development of a more sustainable and resilient citrus industry in Brazil.
Landa, Blanca B.; Castillo, Andreina I.; Giampetruzzi, Annalisa; Kahn, Alexandra; Román-Écija, Miguel; Velasco-Amo, María Pilar; Navas-Cortés, Juan A.; Marco-Noales, Ester; Barbé, Silvia; Moralejo, Eduardo; Coletta-Filho, Helvecio D.; Saldarelli, Pasquale; Saponari, Maria; Almeida, Rodrigo P. P.
Emergence of a plant pathogen in europe associated with multiple intercontinental introductions Journal Article
Em: Applied and Environmental Microbiology, vol. 86, iss. 3, 2020, ISSN: 10985336.
Resumo | Links | BibTeX | Tags: Emerging disease, Genomic diversity, Outbreaks, Quarantine, Recombination, Xylella fastidiosa
@article{Landa2020,
title = {Emergence of a plant pathogen in europe associated with multiple intercontinental introductions},
author = {Blanca B. Landa and Andreina I. Castillo and Annalisa Giampetruzzi and Alexandra Kahn and Miguel Román-Écija and María Pilar Velasco-Amo and Juan A. Navas-Cortés and Ester Marco-Noales and Silvia Barbé and Eduardo Moralejo and Helvecio D. Coletta-Filho and Pasquale Saldarelli and Maria Saponari and Rodrigo P. P. Almeida},
url = {https://journals.asm.org/doi/10.1128/AEM.01521-19},
doi = {10.1128/AEM.01521-19/SUPPL_FILE/ZAM003209563SD1.XLSX},
issn = {10985336},
year = {2020},
date = {2020-01-01},
journal = {Applied and Environmental Microbiology},
volume = {86},
issue = {3},
publisher = {American Society for Microbiology},
abstract = {Pathogen introductions have led to numerous disease outbreaks in naive regions of the globe. The plant pathogen Xylella fastidiosa has been associated with various recent epidemics in Europe affecting agricultural crops, such as almond, grapevine, and olive, but also endemic species occurring in natural forest landscapes and ornamental plants. We compared whole-genome sequences of X. fastidiosa subspecies multiplex from America and strains associated with recent outbreaks in southern Europe to infer their likely origins and paths of introduction within and between the two continents. Phylogenetic analyses indicated multiple introductions of X. fastidiosa subspecies multiplex into Italy, Spain, and France, most of which emerged from a clade with limited genetic diversity with a likely origin in California, USA. The limited genetic diversity observed in X. fastidiosa subspecies multiplex strains originating from California is likely due to the clade itself being an introduction from X. fastidiosa subspecies multiplex populations in the southeastern United States, where this subspecies is most likely endemic. Despite the genetic diversity found in some areas in Europe, there was no clear evidence of recombination occurring among introduced X. fastidiosa strains in Europe. Sequence type taxonomy, based on multilocus sequence typing (MLST), was shown, at least in one case, to not lead to monophyletic clades of this pathogen; whole-genome sequence data were more informative in resolving the history of introductions than MLST data. Although additional data are necessary to carefully tease out the paths of these recent dispersal events, our results indicate that whole-genome sequence data should be considered when developing management strategies for X. fastidiosa outbreaks. IMPORTANCE Xylella fastidiosa is an economically important plant-pathogenic bacterium that has emerged as a pathogen of global importance associated with a devastating epidemic in olive trees in Italy associated with X. fastidiosa subspecies pauca and other outbreaks in Europe, such as X. fastidiosa subspecies fastidiosa and X. fastidiosa subspecies multiplex in Spain and X. fastidiosa subspecies multiplex in France. We present evidence of multiple introductions of X. fastidiosa subspecies multiplex, likely from the United States, into Spain, Italy, and France. These introductions illustrate the risks associated with the commercial trade of plant material at global scales and the need to develop effective policy to limit the likelihood of pathogen pollution into naive regions. Our study demonstrates the need to utilize whole-genome sequence data to study X. fastidiosa introductions at outbreak stages, since a limited number of genetic markers does not provide sufficient phylogenetic resolution to determine dispersal paths or relationships among strains that are of biological and quarantine relevance.},
keywords = {Emerging disease, Genomic diversity, Outbreaks, Quarantine, Recombination, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Pathogen introductions have led to numerous disease outbreaks in naive regions of the globe. The plant pathogen Xylella fastidiosa has been associated with various recent epidemics in Europe affecting agricultural crops, such as almond, grapevine, and olive, but also endemic species occurring in natural forest landscapes and ornamental plants. We compared whole-genome sequences of X. fastidiosa subspecies multiplex from America and strains associated with recent outbreaks in southern Europe to infer their likely origins and paths of introduction within and between the two continents. Phylogenetic analyses indicated multiple introductions of X. fastidiosa subspecies multiplex into Italy, Spain, and France, most of which emerged from a clade with limited genetic diversity with a likely origin in California, USA. The limited genetic diversity observed in X. fastidiosa subspecies multiplex strains originating from California is likely due to the clade itself being an introduction from X. fastidiosa subspecies multiplex populations in the southeastern United States, where this subspecies is most likely endemic. Despite the genetic diversity found in some areas in Europe, there was no clear evidence of recombination occurring among introduced X. fastidiosa strains in Europe. Sequence type taxonomy, based on multilocus sequence typing (MLST), was shown, at least in one case, to not lead to monophyletic clades of this pathogen; whole-genome sequence data were more informative in resolving the history of introductions than MLST data. Although additional data are necessary to carefully tease out the paths of these recent dispersal events, our results indicate that whole-genome sequence data should be considered when developing management strategies for X. fastidiosa outbreaks. IMPORTANCE Xylella fastidiosa is an economically important plant-pathogenic bacterium that has emerged as a pathogen of global importance associated with a devastating epidemic in olive trees in Italy associated with X. fastidiosa subspecies pauca and other outbreaks in Europe, such as X. fastidiosa subspecies fastidiosa and X. fastidiosa subspecies multiplex in Spain and X. fastidiosa subspecies multiplex in France. We present evidence of multiple introductions of X. fastidiosa subspecies multiplex, likely from the United States, into Spain, Italy, and France. These introductions illustrate the risks associated with the commercial trade of plant material at global scales and the need to develop effective policy to limit the likelihood of pathogen pollution into naive regions. Our study demonstrates the need to utilize whole-genome sequence data to study X. fastidiosa introductions at outbreak stages, since a limited number of genetic markers does not provide sufficient phylogenetic resolution to determine dispersal paths or relationships among strains that are of biological and quarantine relevance.
2019
Vanhove, Mathieu; Retchless, Adam C.; Sicard, Anne; Rieux, Adrien; Coletta-Filho, Helvecio D.; Fuente, Leonardo De La; Stenger, Drake C.; Almeida, Rodrigo P. P.
Genomic diversity and recombination among Xylella fastidiosa subspecies Journal Article
Em: Applied and Environmental Microbiology, vol. 85, iss. 13, pp. 2972-2990, 2019, ISSN: 10985336.
Resumo | Links | BibTeX | Tags: Emerging disease, Genomic diversity, Recombination, Xylella fastidiosa
@article{Vanhove2019,
title = {Genomic diversity and recombination among Xylella fastidiosa subspecies},
author = {Mathieu Vanhove and Adam C. Retchless and Anne Sicard and Adrien Rieux and Helvecio D. Coletta-Filho and Leonardo De La Fuente and Drake C. Stenger and Rodrigo P. P. Almeida},
url = {https://journals.asm.org/doi/10.1128/AEM.02972-18},
doi = {10.1128/AEM.02972-18/SUPPL_FILE/AEM.02972-18-S0001.PDF},
issn = {10985336},
year = {2019},
date = {2019-01-01},
journal = {Applied and Environmental Microbiology},
volume = {85},
issue = {13},
pages = {2972-2990},
publisher = {American Society for Microbiology},
abstract = {Xylella fastidiosa is an economically important bacterial plant pathogen. With insights gained from 72 genomes, this study investigated differences among the three main subspecies, which have allopatric origins: X. fastidiosa subsp. fastidiosa, multiplex, and pauca. The origin of recombinogenic X. fastidiosa subsp. morus and sandyi was also assessed. The evolutionary rate of the 622 genes of the species core genome was estimated at the scale of an X. fastidiosa subsp. pauca subclade (7.62×10-7 substitutions per site per year), which was subsequently used to estimate divergence time for the subspecies and introduction events. The study characterized genes present in the accessory genome of each of the three subspecies and investigated the core genome to detect genes potentially under positive selection. Recombination is recognized to be the major driver of diversity in X. fastidiosa, potentially facilitating shifts to novel plant hosts. The relative effect of recombination in comparison to point mutation was calculated (r/m=2.259). Evidence of recombination was uncovered in the core genome alignment; X. fastidiosa subsp. fastidiosa in the United States was less prone to recombination, with an average of 3.22 of the 622 core genes identified as recombining regions, whereas a specific clade of X. fastidiosa subsp. multiplex was found to have on average 9.60 recombining genes, 93.2% of which originated from X. fastidiosa subsp. fastidiosa. Interestingly, for X. fastidiosa subsp. morus, which was initially thought to be the outcome of genome-wide recombination between X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex, intersubspecies homologous recombination levels reached 15.30% in the core genome. Finally, there is evidence of X. fastidiosa subsp. pauca strains from citrus containing genetic elements acquired from strains infecting coffee plants as well as genetic elements from both X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex. In summary, our data provide new insights into the evolution and epidemiology of this plant pathogen.},
keywords = {Emerging disease, Genomic diversity, Recombination, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Xylella fastidiosa is an economically important bacterial plant pathogen. With insights gained from 72 genomes, this study investigated differences among the three main subspecies, which have allopatric origins: X. fastidiosa subsp. fastidiosa, multiplex, and pauca. The origin of recombinogenic X. fastidiosa subsp. morus and sandyi was also assessed. The evolutionary rate of the 622 genes of the species core genome was estimated at the scale of an X. fastidiosa subsp. pauca subclade (7.62×10-7 substitutions per site per year), which was subsequently used to estimate divergence time for the subspecies and introduction events. The study characterized genes present in the accessory genome of each of the three subspecies and investigated the core genome to detect genes potentially under positive selection. Recombination is recognized to be the major driver of diversity in X. fastidiosa, potentially facilitating shifts to novel plant hosts. The relative effect of recombination in comparison to point mutation was calculated (r/m=2.259). Evidence of recombination was uncovered in the core genome alignment; X. fastidiosa subsp. fastidiosa in the United States was less prone to recombination, with an average of 3.22 of the 622 core genes identified as recombining regions, whereas a specific clade of X. fastidiosa subsp. multiplex was found to have on average 9.60 recombining genes, 93.2% of which originated from X. fastidiosa subsp. fastidiosa. Interestingly, for X. fastidiosa subsp. morus, which was initially thought to be the outcome of genome-wide recombination between X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex, intersubspecies homologous recombination levels reached 15.30% in the core genome. Finally, there is evidence of X. fastidiosa subsp. pauca strains from citrus containing genetic elements acquired from strains infecting coffee plants as well as genetic elements from both X. fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex. In summary, our data provide new insights into the evolution and epidemiology of this plant pathogen.
2018
Silva, Aldeliane M.; Sahoo, Prasana K.; Cavalli, Alessandro; Souza, Alessandra A.; Bakkers, Erik P. A. M.; Cesar, Carlos L.; Janissen, Richard; Cotta, Monica A.
Em: Small Methods, vol. 2, iss. 7, pp. 1700411, 2018, ISSN: 2366-9608.
Resumo | Links | BibTeX | Tags: Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa
@article{nokey,
title = {Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces},
author = {Aldeliane M. Silva and Prasana K. Sahoo and Alessandro Cavalli and Alessandra A. Souza and Erik P. A. M. Bakkers and Carlos L. Cesar and Richard Janissen and Monica A. Cotta},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/10.1002/smtd.201700411},
doi = {10.1002/SMTD.201700411},
issn = {2366-9608},
year = {2018},
date = {2018-01-01},
journal = {Small Methods},
volume = {2},
issue = {7},
pages = {1700411},
publisher = {John Wiley & Sons, Ltd},
abstract = {The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.},
keywords = {Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.
Silva, Aldeliane M.; Sahoo, Prasana K.; Cavalli, Alessandro; Souza, Alessandra A.; Bakkers, Erik P. A. M.; Cesar, Carlos L.; Janissen, Richard; Cotta, Monica A.
Em: Small Methods, vol. 2, iss. 7, pp. 1700411, 2018, ISSN: 2366-9608.
Resumo | Links | BibTeX | Tags: Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa
@article{nokey,
title = {Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces},
author = {Aldeliane M. Silva and Prasana K. Sahoo and Alessandro Cavalli and Alessandra A. Souza and Erik P. A. M. Bakkers and Carlos L. Cesar and Richard Janissen and Monica A. Cotta},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.201700411 https://onlinelibrary.wiley.com/doi/10.1002/smtd.201700411},
doi = {10.1002/SMTD.201700411},
issn = {2366-9608},
year = {2018},
date = {2018-01-01},
journal = {Small Methods},
volume = {2},
issue = {7},
pages = {1700411},
publisher = {John Wiley & Sons, Ltd},
abstract = {The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.},
keywords = {Biofilms, cell adhesion forces, confocal microscopy, nanowires, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N-acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined.
2017
Silva, Michelli Massaroli Da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima Fernandes Da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves De
Em: Molecules 2017, Vol. 22, Page 985, vol. 22, iss. 6, pp. 985, 2017, ISSN: 1420-3049.
Resumo | Links | BibTeX | Tags: diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa
@article{nokey,
title = {A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry},
author = {Michelli Massaroli Da Silva and Moacir Dos Santos Andrade and Anelize Bauermeister and Marcus Vinícius Merfa and Moacir Rossi Forim and João Batista Fernandes and Paulo Cezar Vieira and Maria Fátima Fernandes Da Silva and Norberto Peporine Lopes and Marcos Antônio Machado and Alessandra Alves De Souza},
url = {https://www.mdpi.com/1420-3049/22/6/985/htm https://www.mdpi.com/1420-3049/22/6/985},
doi = {10.3390/MOLECULES22060985},
issn = {1420-3049},
year = {2017},
date = {2017-01-01},
journal = {Molecules 2017, Vol. 22, Page 985},
volume = {22},
issue = {6},
pages = {985},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.},
keywords = {diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.
Silva, Michelli Massaroli Da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima Fernandes Da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves De
Em: Molecules 2017, Vol. 22, Page 985, vol. 22, iss. 6, pp. 985, 2017, ISSN: 1420-3049.
Resumo | Links | BibTeX | Tags: diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa
@article{nokey,
title = {A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry},
author = {Michelli Massaroli Da Silva and Moacir Dos Santos Andrade and Anelize Bauermeister and Marcus Vinícius Merfa and Moacir Rossi Forim and João Batista Fernandes and Paulo Cezar Vieira and Maria Fátima Fernandes Da Silva and Norberto Peporine Lopes and Marcos Antônio Machado and Alessandra Alves De Souza},
url = {https://www.mdpi.com/1420-3049/22/6/985/htm https://www.mdpi.com/1420-3049/22/6/985},
doi = {10.3390/MOLECULES22060985},
issn = {1420-3049},
year = {2017},
date = {2017-01-01},
journal = {Molecules 2017, Vol. 22, Page 985},
volume = {22},
issue = {6},
pages = {985},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.},
keywords = {diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.
Silva, Michelli Massaroli Da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima Fernandes Da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves De
Em: Molecules 2017, Vol. 22, Page 985, vol. 22, iss. 6, pp. 985, 2017, ISSN: 1420-3049.
Resumo | Links | BibTeX | Tags: diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa
@article{nokey,
title = {A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry},
author = {Michelli Massaroli Da Silva and Moacir Dos Santos Andrade and Anelize Bauermeister and Marcus Vinícius Merfa and Moacir Rossi Forim and João Batista Fernandes and Paulo Cezar Vieira and Maria Fátima Fernandes Da Silva and Norberto Peporine Lopes and Marcos Antônio Machado and Alessandra Alves De Souza},
url = {https://www.mdpi.com/1420-3049/22/6/985/htm https://www.mdpi.com/1420-3049/22/6/985},
doi = {10.3390/MOLECULES22060985},
issn = {1420-3049},
year = {2017},
date = {2017-01-01},
journal = {Molecules 2017, Vol. 22, Page 985},
volume = {22},
issue = {6},
pages = {985},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.},
keywords = {diketopiperazines, electrospray ionization, Fast Liquid Chromatograph, mass spectrometry, Ultra, X. fastidiosa medium XFM, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.
Monteiro, Moniellen P.; Clerici, João H.; Sahoo, Prasana K.; Cesar, Carlos L.; Souza, Alessandra A.; Cotta, Mônica A.
Stiffness signatures along early stages of Xylella fastidiosa biofilm formation Journal Article
Em: Colloids and Surfaces B: Biointerfaces, vol. 159, pp. 174-182, 2017, ISSN: 0927-7765.
Resumo | Links | BibTeX | Tags: Biofilm, Extracellular polymeric substances, Stiffness, Xylella fastidiosa
@article{Monteiro2017,
title = {Stiffness signatures along early stages of Xylella fastidiosa biofilm formation},
author = {Moniellen P. Monteiro and João H. Clerici and Prasana K. Sahoo and Carlos L. Cesar and Alessandra A. Souza and Mônica A. Cotta},
doi = {10.1016/J.COLSURFB.2017.07.075},
issn = {0927-7765},
year = {2017},
date = {2017-01-01},
journal = {Colloids and Surfaces B: Biointerfaces},
volume = {159},
pages = {174-182},
publisher = {Elsevier},
abstract = {The pathogenicity of Xylella fastidiosa is associated with its systematic colonization of the plant xylem, forming bacterial biofilms. Mechanisms of bacterial transport among different xylem vessels, however, are not completely understood yet, but are strongly influenced by the presence of extracellular polymeric substances (EPS), which surrounds the assembly of cells forming the biofilm. In this work, we show quantitative measurements on the elastic properties of the system composed by EPS and bacterial cell. In order to investigate the mechanical properties of this system, force spectroscopy and confocal Raman measurements were carried out during Xylella fastidiosa subsp. pauca initial stages of adhesion and cluster formation. We show that stiffness progressively decreases with increasing culture growth time, from two to five days. For early adhesion samples, stiffness values are quite different at the bacterial polar and body regions. Lower stiffness values at the cell pole suggest a flexible mechanical response at this region, associated with first cell adhesion to a surface. These results correlate very well with our observations of cell motion within microchannels, under conditions simulating xylem flow. Both the oscillatory movement of vertically attached single cells, as well as the transport of cell clusters within the biofilm matrix can be explained by the presence of softer materials at the cell pole and EPS matrix. Our results may thus add to a more detailed understanding of mechanisms used by cells to migrate among vessels in plant xylem.},
keywords = {Biofilm, Extracellular polymeric substances, Stiffness, Xylella fastidiosa},
pubstate = {published},
tppubtype = {article}
}
The pathogenicity of Xylella fastidiosa is associated with its systematic colonization of the plant xylem, forming bacterial biofilms. Mechanisms of bacterial transport among different xylem vessels, however, are not completely understood yet, but are strongly influenced by the presence of extracellular polymeric substances (EPS), which surrounds the assembly of cells forming the biofilm. In this work, we show quantitative measurements on the elastic properties of the system composed by EPS and bacterial cell. In order to investigate the mechanical properties of this system, force spectroscopy and confocal Raman measurements were carried out during Xylella fastidiosa subsp. pauca initial stages of adhesion and cluster formation. We show that stiffness progressively decreases with increasing culture growth time, from two to five days. For early adhesion samples, stiffness values are quite different at the bacterial polar and body regions. Lower stiffness values at the cell pole suggest a flexible mechanical response at this region, associated with first cell adhesion to a surface. These results correlate very well with our observations of cell motion within microchannels, under conditions simulating xylem flow. Both the oscillatory movement of vertically attached single cells, as well as the transport of cell clusters within the biofilm matrix can be explained by the presence of softer materials at the cell pole and EPS matrix. Our results may thus add to a more detailed understanding of mechanisms used by cells to migrate among vessels in plant xylem.