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2022
Arena, Gabriella D.; Ramos-González, Pedro L.; Tassi, Aline D.; Machado, Marcos A.; Freitas-Astúa, Juliana
Em: Tropical Plant Pathology, pp. 1-12, 2022, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Plant Pathology
@article{Arena2022,
title = {A TaqMan RT-qPCR assay for absolute quantification of citrus leprosis virus C lineage SJP: disclosing the subgenomic/genomic ratio in plant and mite vector, plant organ-specific viral loads, and the kinetics of viral accumulation in plants},
author = {Gabriella D. Arena and Pedro L. Ramos-González and Aline D. Tassi and Marcos A. Machado and Juliana Freitas-Astúa},
url = {https://link.springer.com/article/10.1007/s40858-022-00539-4},
doi = {10.1007/S40858-022-00539-4/METRICS},
issn = {19832052},
year = {2022},
date = {2022-01-01},
journal = {Tropical Plant Pathology},
pages = {1-12},
publisher = {Springer Science and Business Media LLC},
abstract = {Citrus leprosis virus C (CiLV-C) causes citrus leprosis, a re-emergent viral disease affecting citrus production in Latin America. Here, we developed two TaqMan RT-qPCR assays to detect and quantify CiLV-C lineage SJP, prevalent in the Brazilian citrus belt and the world’s main sweet orange production area. Assays targeted sequences within the genes p29 and RdRp. ORF p29 is transcribed in a subgenomic RNA (sgRNA) and codes for the putative capsid protein. ORF RdRp, coding for the replicase, is directly translated from the genomic RNA (gRNA). After assessing the efficiency and sensitivity of the assays, the targets were quantified in (i) symptomatic tissues of field-collected sweet orange (Citrus sinensis) samples, in a time course after infection in both (ii) Arabidopsis thaliana and (iii) sweet orange plants, and in (iv) the mite vector Brevipalpus yothersi. Sweet orange fruits support higher quantities of CiLV-C molecules than stems and leaves. Amounts of viral molecules in late lesions of different developmental stages collected in the field remain stable, but CiLV-C quantities progressively increase from the early stages of the infection to the appearance of symptoms in both A. thaliana and C. sinensis. The high sensibility of the assays allowed the quantification of CiLV-C in early infection periods, even during the asymptomatic period of plants, and in scant amounts of B. yothersi individuals. In plants, a higher accumulation of p29 than RdRp was reported (sgRNA/gRNA up to 95), reflecting the transcription of the p29 sgRNA. In mites, p29 quantities were only slightly higher than RdRp (sgRNA/gRNA of 2), adding a new tool to evaluate the putative replication of CiLV-C in its vector, a challenging aspect of the study of mite-virus interplay. The methods developed here contribute to a more accurate analysis of citrus leprosis epidemiology and shed light on unknown features of the virus-vector interaction.},
keywords = {Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
Citrus leprosis virus C (CiLV-C) causes citrus leprosis, a re-emergent viral disease affecting citrus production in Latin America. Here, we developed two TaqMan RT-qPCR assays to detect and quantify CiLV-C lineage SJP, prevalent in the Brazilian citrus belt and the world’s main sweet orange production area. Assays targeted sequences within the genes p29 and RdRp. ORF p29 is transcribed in a subgenomic RNA (sgRNA) and codes for the putative capsid protein. ORF RdRp, coding for the replicase, is directly translated from the genomic RNA (gRNA). After assessing the efficiency and sensitivity of the assays, the targets were quantified in (i) symptomatic tissues of field-collected sweet orange (Citrus sinensis) samples, in a time course after infection in both (ii) Arabidopsis thaliana and (iii) sweet orange plants, and in (iv) the mite vector Brevipalpus yothersi. Sweet orange fruits support higher quantities of CiLV-C molecules than stems and leaves. Amounts of viral molecules in late lesions of different developmental stages collected in the field remain stable, but CiLV-C quantities progressively increase from the early stages of the infection to the appearance of symptoms in both A. thaliana and C. sinensis. The high sensibility of the assays allowed the quantification of CiLV-C in early infection periods, even during the asymptomatic period of plants, and in scant amounts of B. yothersi individuals. In plants, a higher accumulation of p29 than RdRp was reported (sgRNA/gRNA up to 95), reflecting the transcription of the p29 sgRNA. In mites, p29 quantities were only slightly higher than RdRp (sgRNA/gRNA of 2), adding a new tool to evaluate the putative replication of CiLV-C in its vector, a challenging aspect of the study of mite-virus interplay. The methods developed here contribute to a more accurate analysis of citrus leprosis epidemiology and shed light on unknown features of the virus-vector interaction.
2020
Souza, Alessandra Alves; Wang, Nian; Coletta-Filho, Helvecio Della; Ponte, Emerson M. Del
Special issue on bacterial citrus diseases: part I Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 3, pp. 163-165, 2020, ISSN: 19832052.
Links | BibTeX | Tags: Plant Pathology
@article{nokey,
title = {Special issue on bacterial citrus diseases: part I},
author = {Alessandra Alves Souza and Nian Wang and Helvecio Della Coletta-Filho and Emerson M. Del Ponte},
url = {https://link.springer.com/article/10.1007/s40858-020-00384-3},
doi = {10.1007/S40858-020-00384-3/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {3},
pages = {163-165},
publisher = {Springer},
keywords = {Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
Souza, Alessandra Alves; Wang, Nian; Coletta-Filho, Helvecio Della; Ponte, Emerson Medeiros Del
Special issue on bacterial citrus diseases: part II Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 6, pp. 557-558, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Plant Pathology
@article{nokey,
title = {Special issue on bacterial citrus diseases: part II},
author = {Alessandra Alves Souza and Nian Wang and Helvecio Della Coletta-Filho and Emerson Medeiros Del Ponte},
url = {https://link.springer.com/article/10.1007/s40858-020-00406-0},
doi = {10.1007/S40858-020-00406-0/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {557-558},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {We are delighted to deliver the second part of our Special Issue focused on bacterial citrus diseases, which completes the series. This part II is almost entirely dedicated to HLB-11 out of 15 articles. We start with the three remaining, all addressing HLB, among which two of them focus on Diaphorina citri (Asian citrus psyllid), the vector of Candidatus Liberibacter asiaticus (CLas), and its relationship with the HLB bacterium. Galdeano and co-workers addressed the impact of CLas on the vector including fitness and behavior , morphology, immune system, metabolism, and microbiome. One of the key findings was that CLas enhances the reproduction and dissemination of psyllids, thus favoring bacteria spread and accumulation on Wolbachia, an endosym-biont that helps to increase the frequency of oviposition by the psyllid female. Transmission mechanism of CLas by the psyl-lid, as well as the effect of environment on biology, ecology, and behavior of the vector, was discussed by Carmo-Sousa. Factors such as the short acquisition and inoculation time (15 to 30 min), the long retention time (8 weeks or entire life), the short latency period and long incubation time, and the efficient mobility of winged vector make vector/disease control more difficult but extremely important. However, understanding of transmission mechanisms and insect behavior in the environment is necessary to avoid or stop disease spread. Another important component, not often considered in disease management programs, is the nutritional status of the host. The effect of nutrient application to maintain or enhance yield in HLB-affected plants is a controversial topic and is reviewed by Mattos Jr. and collaborators. A critical assessment of literature data on nutrient management practices aimed at maintaining fruit production, and quality is made under HLB-free as well as under conditions of low or high HLB incidence. The impact of the disease and the potential beneficial effects of nutritional programs on plant physiology, cell anatomy, and nutrient imbalance are reviewed and discussed. The original research articles on HLB cover several aspects. The work by da Silva and collaborators investigated the effect of supplementation with micronutrients (Zn, Mn, and Cu) on HLB-affected plants as well on the CLas acquisition by the vector. Although not reducing CLas titers in plants, micronutrients mitigated the deleterious effects of HLB on starch metabolism. The combination of Zn, Mn, and Cu, which reduced the acquisition of CLas by adults and especially nymphs of D. citri, opens possibilities for reducing disease spread in citrus groves. Another way of reducing HLB incidence is to use rootstock with dwarfing characteristics and that emit few and homogenous flush shoots, thus facilitating psyl-lid control. Despite the susceptibility of dwarfing "Flying Dragon" rootstock to CLas, Rodrigues and co-workers report reduced levels of HLB incidence and disease progress rate in "Valencia" sweet orange during 8 years of experimentation, encouraging studies using less CLas-susceptible and dwarfing rootstocks. CLas infects all commercial citrus varieties and is present in most of the plant organs, including flower and seed. Even though the vertical transmission of CLas by seeds was not demonstrated so far, Morelli and co-authors showed that seed physiology, germination, and seedlings size for two root-stocks (Rangpur Lime and Swingle citrumelo) were negatively affected by CLas. Although all commercial citrus varieties are susceptible to CLas, Soratto and collaborators report differential responses to HLB in a progeny of hybrids from the cross of Poncirus trifoliata and Sunki mandarin. The authors found that QTL and eQTL were associated with starch accumulation caused by CLas infection. Molecular mechanisms involved in CLas},
keywords = {Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
We are delighted to deliver the second part of our Special Issue focused on bacterial citrus diseases, which completes the series. This part II is almost entirely dedicated to HLB-11 out of 15 articles. We start with the three remaining, all addressing HLB, among which two of them focus on Diaphorina citri (Asian citrus psyllid), the vector of Candidatus Liberibacter asiaticus (CLas), and its relationship with the HLB bacterium. Galdeano and co-workers addressed the impact of CLas on the vector including fitness and behavior , morphology, immune system, metabolism, and microbiome. One of the key findings was that CLas enhances the reproduction and dissemination of psyllids, thus favoring bacteria spread and accumulation on Wolbachia, an endosym-biont that helps to increase the frequency of oviposition by the psyllid female. Transmission mechanism of CLas by the psyl-lid, as well as the effect of environment on biology, ecology, and behavior of the vector, was discussed by Carmo-Sousa. Factors such as the short acquisition and inoculation time (15 to 30 min), the long retention time (8 weeks or entire life), the short latency period and long incubation time, and the efficient mobility of winged vector make vector/disease control more difficult but extremely important. However, understanding of transmission mechanisms and insect behavior in the environment is necessary to avoid or stop disease spread. Another important component, not often considered in disease management programs, is the nutritional status of the host. The effect of nutrient application to maintain or enhance yield in HLB-affected plants is a controversial topic and is reviewed by Mattos Jr. and collaborators. A critical assessment of literature data on nutrient management practices aimed at maintaining fruit production, and quality is made under HLB-free as well as under conditions of low or high HLB incidence. The impact of the disease and the potential beneficial effects of nutritional programs on plant physiology, cell anatomy, and nutrient imbalance are reviewed and discussed. The original research articles on HLB cover several aspects. The work by da Silva and collaborators investigated the effect of supplementation with micronutrients (Zn, Mn, and Cu) on HLB-affected plants as well on the CLas acquisition by the vector. Although not reducing CLas titers in plants, micronutrients mitigated the deleterious effects of HLB on starch metabolism. The combination of Zn, Mn, and Cu, which reduced the acquisition of CLas by adults and especially nymphs of D. citri, opens possibilities for reducing disease spread in citrus groves. Another way of reducing HLB incidence is to use rootstock with dwarfing characteristics and that emit few and homogenous flush shoots, thus facilitating psyl-lid control. Despite the susceptibility of dwarfing "Flying Dragon" rootstock to CLas, Rodrigues and co-workers report reduced levels of HLB incidence and disease progress rate in "Valencia" sweet orange during 8 years of experimentation, encouraging studies using less CLas-susceptible and dwarfing rootstocks. CLas infects all commercial citrus varieties and is present in most of the plant organs, including flower and seed. Even though the vertical transmission of CLas by seeds was not demonstrated so far, Morelli and co-authors showed that seed physiology, germination, and seedlings size for two root-stocks (Rangpur Lime and Swingle citrumelo) were negatively affected by CLas. Although all commercial citrus varieties are susceptible to CLas, Soratto and collaborators report differential responses to HLB in a progeny of hybrids from the cross of Poncirus trifoliata and Sunki mandarin. The authors found that QTL and eQTL were associated with starch accumulation caused by CLas infection. Molecular mechanisms involved in CLas
Souza, Alessandra Alves; Wang, Nian; Coletta-Filho, Helvecio Della; Ponte, Emerson M. Del
Special issue on bacterial citrus diseases: part I Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 3, pp. 163-165, 2020, ISSN: 19832052.
Links | BibTeX | Tags: Plant Pathology
@article{nokey,
title = {Special issue on bacterial citrus diseases: part I},
author = {Alessandra Alves Souza and Nian Wang and Helvecio Della Coletta-Filho and Emerson M. Del Ponte},
url = {https://link.springer.com/article/10.1007/s40858-020-00384-3},
doi = {10.1007/S40858-020-00384-3/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {3},
pages = {163-165},
publisher = {Springer},
keywords = {Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
Souza, Alessandra Alves; Wang, Nian; Coletta-Filho, Helvecio Della; Ponte, Emerson Medeiros Del
Special issue on bacterial citrus diseases: part II Journal Article
Em: Tropical Plant Pathology, vol. 45, iss. 6, pp. 557-558, 2020, ISSN: 19832052.
Resumo | Links | BibTeX | Tags: Plant Pathology
@article{nokey,
title = {Special issue on bacterial citrus diseases: part II},
author = {Alessandra Alves Souza and Nian Wang and Helvecio Della Coletta-Filho and Emerson Medeiros Del Ponte},
url = {https://link.springer.com/article/10.1007/s40858-020-00406-0},
doi = {10.1007/S40858-020-00406-0/METRICS},
issn = {19832052},
year = {2020},
date = {2020-01-01},
journal = {Tropical Plant Pathology},
volume = {45},
issue = {6},
pages = {557-558},
publisher = {Springer Science and Business Media Deutschland GmbH},
abstract = {We are delighted to deliver the second part of our Special Issue focused on bacterial citrus diseases, which completes the series. This part II is almost entirely dedicated to HLB-11 out of 15 articles. We start with the three remaining, all addressing HLB, among which two of them focus on Diaphorina citri (Asian citrus psyllid), the vector of Candidatus Liberibacter asiaticus (CLas), and its relationship with the HLB bacterium. Galdeano and co-workers addressed the impact of CLas on the vector including fitness and behavior , morphology, immune system, metabolism, and microbiome. One of the key findings was that CLas enhances the reproduction and dissemination of psyllids, thus favoring bacteria spread and accumulation on Wolbachia, an endosym-biont that helps to increase the frequency of oviposition by the psyllid female. Transmission mechanism of CLas by the psyl-lid, as well as the effect of environment on biology, ecology, and behavior of the vector, was discussed by Carmo-Sousa. Factors such as the short acquisition and inoculation time (15 to 30 min), the long retention time (8 weeks or entire life), the short latency period and long incubation time, and the efficient mobility of winged vector make vector/disease control more difficult but extremely important. However, understanding of transmission mechanisms and insect behavior in the environment is necessary to avoid or stop disease spread. Another important component, not often considered in disease management programs, is the nutritional status of the host. The effect of nutrient application to maintain or enhance yield in HLB-affected plants is a controversial topic and is reviewed by Mattos Jr. and collaborators. A critical assessment of literature data on nutrient management practices aimed at maintaining fruit production, and quality is made under HLB-free as well as under conditions of low or high HLB incidence. The impact of the disease and the potential beneficial effects of nutritional programs on plant physiology, cell anatomy, and nutrient imbalance are reviewed and discussed. The original research articles on HLB cover several aspects. The work by da Silva and collaborators investigated the effect of supplementation with micronutrients (Zn, Mn, and Cu) on HLB-affected plants as well on the CLas acquisition by the vector. Although not reducing CLas titers in plants, micronutrients mitigated the deleterious effects of HLB on starch metabolism. The combination of Zn, Mn, and Cu, which reduced the acquisition of CLas by adults and especially nymphs of D. citri, opens possibilities for reducing disease spread in citrus groves. Another way of reducing HLB incidence is to use rootstock with dwarfing characteristics and that emit few and homogenous flush shoots, thus facilitating psyl-lid control. Despite the susceptibility of dwarfing "Flying Dragon" rootstock to CLas, Rodrigues and co-workers report reduced levels of HLB incidence and disease progress rate in "Valencia" sweet orange during 8 years of experimentation, encouraging studies using less CLas-susceptible and dwarfing rootstocks. CLas infects all commercial citrus varieties and is present in most of the plant organs, including flower and seed. Even though the vertical transmission of CLas by seeds was not demonstrated so far, Morelli and co-authors showed that seed physiology, germination, and seedlings size for two root-stocks (Rangpur Lime and Swingle citrumelo) were negatively affected by CLas. Although all commercial citrus varieties are susceptible to CLas, Soratto and collaborators report differential responses to HLB in a progeny of hybrids from the cross of Poncirus trifoliata and Sunki mandarin. The authors found that QTL and eQTL were associated with starch accumulation caused by CLas infection. Molecular mechanisms involved in CLas},
keywords = {Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
We are delighted to deliver the second part of our Special Issue focused on bacterial citrus diseases, which completes the series. This part II is almost entirely dedicated to HLB-11 out of 15 articles. We start with the three remaining, all addressing HLB, among which two of them focus on Diaphorina citri (Asian citrus psyllid), the vector of Candidatus Liberibacter asiaticus (CLas), and its relationship with the HLB bacterium. Galdeano and co-workers addressed the impact of CLas on the vector including fitness and behavior , morphology, immune system, metabolism, and microbiome. One of the key findings was that CLas enhances the reproduction and dissemination of psyllids, thus favoring bacteria spread and accumulation on Wolbachia, an endosym-biont that helps to increase the frequency of oviposition by the psyllid female. Transmission mechanism of CLas by the psyl-lid, as well as the effect of environment on biology, ecology, and behavior of the vector, was discussed by Carmo-Sousa. Factors such as the short acquisition and inoculation time (15 to 30 min), the long retention time (8 weeks or entire life), the short latency period and long incubation time, and the efficient mobility of winged vector make vector/disease control more difficult but extremely important. However, understanding of transmission mechanisms and insect behavior in the environment is necessary to avoid or stop disease spread. Another important component, not often considered in disease management programs, is the nutritional status of the host. The effect of nutrient application to maintain or enhance yield in HLB-affected plants is a controversial topic and is reviewed by Mattos Jr. and collaborators. A critical assessment of literature data on nutrient management practices aimed at maintaining fruit production, and quality is made under HLB-free as well as under conditions of low or high HLB incidence. The impact of the disease and the potential beneficial effects of nutritional programs on plant physiology, cell anatomy, and nutrient imbalance are reviewed and discussed. The original research articles on HLB cover several aspects. The work by da Silva and collaborators investigated the effect of supplementation with micronutrients (Zn, Mn, and Cu) on HLB-affected plants as well on the CLas acquisition by the vector. Although not reducing CLas titers in plants, micronutrients mitigated the deleterious effects of HLB on starch metabolism. The combination of Zn, Mn, and Cu, which reduced the acquisition of CLas by adults and especially nymphs of D. citri, opens possibilities for reducing disease spread in citrus groves. Another way of reducing HLB incidence is to use rootstock with dwarfing characteristics and that emit few and homogenous flush shoots, thus facilitating psyl-lid control. Despite the susceptibility of dwarfing "Flying Dragon" rootstock to CLas, Rodrigues and co-workers report reduced levels of HLB incidence and disease progress rate in "Valencia" sweet orange during 8 years of experimentation, encouraging studies using less CLas-susceptible and dwarfing rootstocks. CLas infects all commercial citrus varieties and is present in most of the plant organs, including flower and seed. Even though the vertical transmission of CLas by seeds was not demonstrated so far, Morelli and co-authors showed that seed physiology, germination, and seedlings size for two root-stocks (Rangpur Lime and Swingle citrumelo) were negatively affected by CLas. Although all commercial citrus varieties are susceptible to CLas, Soratto and collaborators report differential responses to HLB in a progeny of hybrids from the cross of Poncirus trifoliata and Sunki mandarin. The authors found that QTL and eQTL were associated with starch accumulation caused by CLas infection. Molecular mechanisms involved in CLas
2017
Máximo, Heros J.; Dalio, Ronaldo J. D.; Rodrigues, Carolina M.; Breton, Michèle C.; Machado, Marcos A.
Reference genes for RT-qPCR analysis in Citrus and Poncirus infected by zoospores of Phytophthora parasitica Journal Article
Em: Tropical Plant Pathology 2017 42:2, vol. 42, iss. 2, pp. 76-85, 2017, ISSN: 1983-2052.
Resumo | Links | BibTeX | Tags: Housekeeping genes, Molecular biology, Oomycetes, Plant Pathology
@article{nokey,
title = {Reference genes for RT-qPCR analysis in Citrus and Poncirus infected by zoospores of Phytophthora parasitica},
author = {Heros J. Máximo and Ronaldo J. D. Dalio and Carolina M. Rodrigues and Michèle C. Breton and Marcos A. Machado},
url = {https://link.springer.com/article/10.1007/s40858-017-0134-8},
doi = {10.1007/S40858-017-0134-8},
issn = {1983-2052},
year = {2017},
date = {2017-01-01},
journal = {Tropical Plant Pathology 2017 42:2},
volume = {42},
issue = {2},
pages = {76-85},
publisher = {Springer},
abstract = {Phytophthora species are highly destructive phytopathogens, associated with massive damage in natural ecosystems and agriculture. Citrus production is also affected, mainly by the hemibiothrophic oomycete Phytophthora parasitica, which causes root rot and gummosis. Poncirus trifoliata and Citrus sunki (two rootstocks widely used in citrus orchards) pose a resistance and a susceptible interaction with P. parasitica, respectively, which makes them suitable models to study plant defense mechanisms. Gene expression analysis is a very important tool in this type of study, in particular PCR (RT-qPCR). Hence, it is crucial to use appropriate reference genes for expression normalization. Our aim was to evaluate the stability of several candidate reference genes to determine which set is best suited for normalization in citrus infected with P. parasitica. We evaluated five candidate reference genes selected from the database CitEST. GeNorm and NormFinder algorithms were used to assess the best reference genes. We found that the more stable genes to be used for RT-qPCR analysis in P. trifoliata plants were GAPC2 and F-BOX, while EGIDH and GAPC2 were more suitable to C. sunki. These four genes were found to be excellent normalizers, being stable throughout the infection regardless of pathogen attack or symptom development.},
keywords = {Housekeeping genes, Molecular biology, Oomycetes, Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
Phytophthora species are highly destructive phytopathogens, associated with massive damage in natural ecosystems and agriculture. Citrus production is also affected, mainly by the hemibiothrophic oomycete Phytophthora parasitica, which causes root rot and gummosis. Poncirus trifoliata and Citrus sunki (two rootstocks widely used in citrus orchards) pose a resistance and a susceptible interaction with P. parasitica, respectively, which makes them suitable models to study plant defense mechanisms. Gene expression analysis is a very important tool in this type of study, in particular PCR (RT-qPCR). Hence, it is crucial to use appropriate reference genes for expression normalization. Our aim was to evaluate the stability of several candidate reference genes to determine which set is best suited for normalization in citrus infected with P. parasitica. We evaluated five candidate reference genes selected from the database CitEST. GeNorm and NormFinder algorithms were used to assess the best reference genes. We found that the more stable genes to be used for RT-qPCR analysis in P. trifoliata plants were GAPC2 and F-BOX, while EGIDH and GAPC2 were more suitable to C. sunki. These four genes were found to be excellent normalizers, being stable throughout the infection regardless of pathogen attack or symptom development.
Waculicz-Andrade, C. E.; Savi, D. C.; Bini, A. P.; Adamoski, D.; Goulin, E. H.; Silva, G. J.; Massola, N. S.; Terasawa, L. G.; Kava, V.; Glienke, Chirlei
Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil? Journal Article
Em: Australasian Plant Pathology 2017 46:2, vol. 46, iss. 2, pp. 191-203, 2017, ISSN: 1448-6032.
Resumo | Links | BibTeX | Tags: agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences
@article{nokey,
title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},
author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},
url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},
doi = {10.1007/S13313-017-0476-1},
issn = {1448-6032},
year = {2017},
date = {2017-01-01},
journal = {Australasian Plant Pathology 2017 46:2},
volume = {46},
issue = {2},
pages = {191-203},
publisher = {Springer},
abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},
keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},
pubstate = {published},
tppubtype = {article}
}
Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.
Máximo, Heros J.; Dalio, Ronaldo J. D.; Rodrigues, Carolina M.; Breton, Michèle C.; Machado, Marcos A.
Reference genes for RT-qPCR analysis in Citrus and Poncirus infected by zoospores of Phytophthora parasitica Journal Article
Em: Tropical Plant Pathology 2017 42:2, vol. 42, iss. 2, pp. 76-85, 2017, ISSN: 1983-2052.
Resumo | Links | BibTeX | Tags: Housekeeping genes, Molecular biology, Oomycetes, Plant Pathology
@article{nokey,
title = {Reference genes for RT-qPCR analysis in Citrus and Poncirus infected by zoospores of Phytophthora parasitica},
author = {Heros J. Máximo and Ronaldo J. D. Dalio and Carolina M. Rodrigues and Michèle C. Breton and Marcos A. Machado},
url = {https://link.springer.com/article/10.1007/s40858-017-0134-8},
doi = {10.1007/S40858-017-0134-8},
issn = {1983-2052},
year = {2017},
date = {2017-01-01},
journal = {Tropical Plant Pathology 2017 42:2},
volume = {42},
issue = {2},
pages = {76-85},
publisher = {Springer},
abstract = {Phytophthora species are highly destructive phytopathogens, associated with massive damage in natural ecosystems and agriculture. Citrus production is also affected, mainly by the hemibiothrophic oomycete Phytophthora parasitica, which causes root rot and gummosis. Poncirus trifoliata and Citrus sunki (two rootstocks widely used in citrus orchards) pose a resistance and a susceptible interaction with P. parasitica, respectively, which makes them suitable models to study plant defense mechanisms. Gene expression analysis is a very important tool in this type of study, in particular PCR (RT-qPCR). Hence, it is crucial to use appropriate reference genes for expression normalization. Our aim was to evaluate the stability of several candidate reference genes to determine which set is best suited for normalization in citrus infected with P. parasitica. We evaluated five candidate reference genes selected from the database CitEST. GeNorm and NormFinder algorithms were used to assess the best reference genes. We found that the more stable genes to be used for RT-qPCR analysis in P. trifoliata plants were GAPC2 and F-BOX, while EGIDH and GAPC2 were more suitable to C. sunki. These four genes were found to be excellent normalizers, being stable throughout the infection regardless of pathogen attack or symptom development.},
keywords = {Housekeeping genes, Molecular biology, Oomycetes, Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
Phytophthora species are highly destructive phytopathogens, associated with massive damage in natural ecosystems and agriculture. Citrus production is also affected, mainly by the hemibiothrophic oomycete Phytophthora parasitica, which causes root rot and gummosis. Poncirus trifoliata and Citrus sunki (two rootstocks widely used in citrus orchards) pose a resistance and a susceptible interaction with P. parasitica, respectively, which makes them suitable models to study plant defense mechanisms. Gene expression analysis is a very important tool in this type of study, in particular PCR (RT-qPCR). Hence, it is crucial to use appropriate reference genes for expression normalization. Our aim was to evaluate the stability of several candidate reference genes to determine which set is best suited for normalization in citrus infected with P. parasitica. We evaluated five candidate reference genes selected from the database CitEST. GeNorm and NormFinder algorithms were used to assess the best reference genes. We found that the more stable genes to be used for RT-qPCR analysis in P. trifoliata plants were GAPC2 and F-BOX, while EGIDH and GAPC2 were more suitable to C. sunki. These four genes were found to be excellent normalizers, being stable throughout the infection regardless of pathogen attack or symptom development.
Waculicz-Andrade, C. E.; Savi, D. C.; Bini, A. P.; Adamoski, D.; Goulin, E. H.; Silva, G. J.; Massola, N. S.; Terasawa, L. G.; Kava, V.; Glienke, Chirlei
Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil? Journal Article
Em: Australasian Plant Pathology 2017 46:2, vol. 46, iss. 2, pp. 191-203, 2017, ISSN: 1448-6032.
Resumo | Links | BibTeX | Tags: agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences
@article{nokey,
title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},
author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},
url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},
doi = {10.1007/S13313-017-0476-1},
issn = {1448-6032},
year = {2017},
date = {2017-01-01},
journal = {Australasian Plant Pathology 2017 46:2},
volume = {46},
issue = {2},
pages = {191-203},
publisher = {Springer},
abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},
keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},
pubstate = {published},
tppubtype = {article}
}
Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.
Máximo, Heros J.; Dalio, Ronaldo J. D.; Rodrigues, Carolina M.; Breton, Michèle C.; Machado, Marcos A.
Reference genes for RT-qPCR analysis in Citrus and Poncirus infected by zoospores of Phytophthora parasitica Journal Article
Em: Tropical Plant Pathology 2017 42:2, vol. 42, iss. 2, pp. 76-85, 2017, ISSN: 1983-2052.
Resumo | Links | BibTeX | Tags: Housekeeping genes, Molecular biology, Oomycetes, Plant Pathology
@article{nokey,
title = {Reference genes for RT-qPCR analysis in Citrus and Poncirus infected by zoospores of Phytophthora parasitica},
author = {Heros J. Máximo and Ronaldo J. D. Dalio and Carolina M. Rodrigues and Michèle C. Breton and Marcos A. Machado},
url = {https://link.springer.com/article/10.1007/s40858-017-0134-8},
doi = {10.1007/S40858-017-0134-8},
issn = {1983-2052},
year = {2017},
date = {2017-01-01},
journal = {Tropical Plant Pathology 2017 42:2},
volume = {42},
issue = {2},
pages = {76-85},
publisher = {Springer},
abstract = {Phytophthora species are highly destructive phytopathogens, associated with massive damage in natural ecosystems and agriculture. Citrus production is also affected, mainly by the hemibiothrophic oomycete Phytophthora parasitica, which causes root rot and gummosis. Poncirus trifoliata and Citrus sunki (two rootstocks widely used in citrus orchards) pose a resistance and a susceptible interaction with P. parasitica, respectively, which makes them suitable models to study plant defense mechanisms. Gene expression analysis is a very important tool in this type of study, in particular PCR (RT-qPCR). Hence, it is crucial to use appropriate reference genes for expression normalization. Our aim was to evaluate the stability of several candidate reference genes to determine which set is best suited for normalization in citrus infected with P. parasitica. We evaluated five candidate reference genes selected from the database CitEST. GeNorm and NormFinder algorithms were used to assess the best reference genes. We found that the more stable genes to be used for RT-qPCR analysis in P. trifoliata plants were GAPC2 and F-BOX, while EGIDH and GAPC2 were more suitable to C. sunki. These four genes were found to be excellent normalizers, being stable throughout the infection regardless of pathogen attack or symptom development.},
keywords = {Housekeeping genes, Molecular biology, Oomycetes, Plant Pathology},
pubstate = {published},
tppubtype = {article}
}
Phytophthora species are highly destructive phytopathogens, associated with massive damage in natural ecosystems and agriculture. Citrus production is also affected, mainly by the hemibiothrophic oomycete Phytophthora parasitica, which causes root rot and gummosis. Poncirus trifoliata and Citrus sunki (two rootstocks widely used in citrus orchards) pose a resistance and a susceptible interaction with P. parasitica, respectively, which makes them suitable models to study plant defense mechanisms. Gene expression analysis is a very important tool in this type of study, in particular PCR (RT-qPCR). Hence, it is crucial to use appropriate reference genes for expression normalization. Our aim was to evaluate the stability of several candidate reference genes to determine which set is best suited for normalization in citrus infected with P. parasitica. We evaluated five candidate reference genes selected from the database CitEST. GeNorm and NormFinder algorithms were used to assess the best reference genes. We found that the more stable genes to be used for RT-qPCR analysis in P. trifoliata plants were GAPC2 and F-BOX, while EGIDH and GAPC2 were more suitable to C. sunki. These four genes were found to be excellent normalizers, being stable throughout the infection regardless of pathogen attack or symptom development.
Waculicz-Andrade, C. E.; Savi, D. C.; Bini, A. P.; Adamoski, D.; Goulin, E. H.; Silva, G. J.; Massola, N. S.; Terasawa, L. G.; Kava, V.; Glienke, Chirlei
Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil? Journal Article
Em: Australasian Plant Pathology 2017 46:2, vol. 46, iss. 2, pp. 191-203, 2017, ISSN: 1448-6032.
Resumo | Links | BibTeX | Tags: agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences
@article{nokey,
title = {Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?},
author = {C. E. Waculicz-Andrade and D. C. Savi and A. P. Bini and D. Adamoski and E. H. Goulin and G. J. Silva and N. S. Massola and L. G. Terasawa and V. Kava and Chirlei Glienke},
url = {https://link.springer.com/article/10.1007/s13313-017-0476-1},
doi = {10.1007/S13313-017-0476-1},
issn = {1448-6032},
year = {2017},
date = {2017-01-01},
journal = {Australasian Plant Pathology 2017 46:2},
volume = {46},
issue = {2},
pages = {191-203},
publisher = {Springer},
abstract = {Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.},
keywords = {agriculture, Ecology, Entomology, Plant Pathology, Plant Sciences},
pubstate = {published},
tppubtype = {article}
}
Postbloom fruit drop (PFD) was first associated with Colletotrichum gloeosporioides until 1990s. Thereafter, the causal agent was reclassified as a species belonging to C. acutatum complex. Recent findings suggested that among the species, within the C. acutatum complex, C. abscissum is the PFD causal agent. Moreover, previous study reported C. abscissum as the most frequent species among isolates of Colletotrichum from blooms showing PFD symptoms. Despite the recent increase in knowledge concern PFD epidemiology, strategies of pathogen survival in citrus leaves remain unclear. Therefore, the aim of this study was to identify the Colletotrichum endophytic species obtained from leaves of sweet orange and weeds from orchards located in Sao Paulo state, Brazil, and to evaluate the ability of these isolates to cause typical PFC symptoms on flowers. Species-specific PCR was used to identify 188 isolates belonging to the C. gloeosporioides complex, which were characterized as C. gloeosporioides sensu stricto based on multilocus sequence analysis. The pathogenicity test indicated that one isolate of C. gloeosporioides sensu stricto, obtained from citrus leaf, was associated with PFD symptoms on inoculated flowers. Lastly, the species associated with PFD may survive on leaf surface, but only C. gloeosporioides sensu stricto is able to colonize endophytically the sweet orange leaves in Brazil.