Publicações

@article{Savi2020,

title = {Dihydroisocoumarins produced by Diaporthe cf. heveae LGMF1631 inhibiting citrus pathogens},

author = {Daiani Cristina Savi and Sandriele Aparecida Noriler and Larissa V. Ponomareva and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke and Khaled A. Shaaban},

url = {https://link.springer.com/article/10.1007/s12223-019-00746-8},

doi = {10.1007/S12223-019-00746-8/METRICS},

issn = {18749356},

year  = {2020},

date = {2020-01-01},

journal = {Folia Microbiologica},

volume = {65},

issue = {2},

pages = {381-392},

publisher = {Springer},

abstract = {Citrus black spot (CBS) and post-bloom fruit drop (PFD), caused by Phyllosticta citricarpa and Colletotrichum abscissum, respectively, are two important citrus diseases worldwide. CBS depreciates the market value and prevents exportation of citrus fruits to Europe. PFD under favorable climatic conditions can cause the abscission of flowers, thereby reducing citrus production by 80%. An ecofriendly alternative to control plant diseases is the use of endophytic microorganisms, or secondary metabolites produced by them. Strain LGMF1631, close related to Diaporthe cf. heveae 1, was isolated from the medicinal plant Stryphnodendron adstringens and showed significant antimicrobial activity, in a previous study. In view of the potential presented by strain LGMF1631, and the absence of chemical data for secondary metabolites produced by D. cf. heveae, we decided to characterize the compounds produced by strain LGMF1631. Based on ITS, TEF1, and TUB phylogenetic analysis, strain LGMF1631 was confirmed to belong to D. cf. heveae 1. Chemical assessment of the fungal strain LGMF1631 revealed one new seco-dihydroisocoumarin [cladosporin B (1)] along with six other related, already known dihydroisocoumarin derivatives and one monoterpene [(−)-(1S,2R,3S,4R)-p-menthane-1,2,3-triol (8)]. Among the isolated metabolites, compound 5 drastically reduced the growth of both phytopathogens in vitro and completely inhibited the development of CBS and PFD in citrus fruits and flowers. In addition, compound 5 did not show toxicity against human cancer cell lines or citrus leaves, at concentrations higher than used for the inhibition of the phytopathogens, suggesting the potential use of (−)-(3R,4R)-cis-4-hydroxy-5-methylmellein (5) to control citrus diseases.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{Savi2019,

title = {Secondary metabolites produced by Microbacterium sp. LGMB471 with antifungal activity against the phytopathogen Phyllosticta citricarpa},

author = {Daiani Cristina Savi and Jürgen Rohr and Khaled A. Shaaban and Francielly M. W. Gos and Jon S. Thorson and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-00668-x},

doi = {10.1007/S12223-018-00668-X/METRICS},

issn = {18749356},

year  = {2019},

date = {2019-01-01},

journal = {Folia Microbiologica},

volume = {64},

issue = {3},

pages = {453-460},

publisher = {Springer Science and Business Media B.V.},

abstract = {The citrus black spot (CBS), caused by Phyllosticta citricarpa, is one of the most important citrus diseases in subtropical regions of Africa, Asia, Oceania, and the Americas, and fruits with CBS lesions are still subject to quarantine regulations in the European Union. Despite the high application of fungicides, the disease remains present in the citrus crops of Central and South America. In order to find alternatives to help control CBS and reduce the use of fungicides, we explored the antifungal potential of endophytic actinomycetes isolated from the Brazilian medicinal plant Vochysia divergens found in the Pantanal biome. Two different culture media and temperatures were selected to identify the most efficient conditions for the production of active secondary metabolites. The metabolites produced by strain Microbacterium sp. LGMB471 cultured in SG medium at 36 °C considerably inhibited the development of P. citricarpa. Three isoflavones and five diketopiperazines were identified, and the compounds 7-O-β-D-glucosyl-genistein and 7-O-β-D-glucosyl-daidzein showed high activity against P. citricarpa, with the MIC of 33 μg/mL and inhibited the production of asexual spores of P. citricarpa on leaves and citrus fruits. Compounds that inhibit conidia formation may be a promising alternative to reduce the use of fungicides in the control of CBS lesions, especially in regions where sexual reproduction does not occur, as in the USA. Our data suggest the use of Microbacterium sp. LGMB471 or its metabolites as an ecological alternative to be used in association with the fungicides for the control of CBS disease.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{Hippler2018e,

title = {Mechanisms of copper stress alleviation in Citrus trees after metal uptake by leaves or roots},

author = {Franz Walter Rieger Hippler and Guilherme Petená and Rodrigo Marcelli Boaretto and José Antônio Quaggio and Ricardo Antunes Azevedo and Dirceu Mattos-Jr},

url = {https://link.springer.com/article/10.1007/s11356-018-1529-x},

doi = {10.1007/S11356-018-1529-X},

issn = {1614-7499},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research 2018 25:13},

volume = {25},

issue = {13},

pages = {13134-13146},

publisher = {Springer},

abstract = {Nutritional disorders caused by copper (Cu) have affected citrus orchards. Since Cu is foliar sprayed as a pesticide to control citrus diseases, this metal accumulates in the soil. Thereby, we evaluated the effects of Cu leaf absorption after spray of different metal sources, as well as roots absorption on growth, nutritional status, and oxidative stress of young sweet orange trees. Two experiments were carried out under greenhouse conditions. The first experiment was set up with varying Cu levels to the soil (nil Cu, 0.5, 2.0, 4.0 and 8.0 g of Cu per plant as CuSO4.5H2O), whereas the second experiment with Cu application via foliar sprays (0.5 and 2.0 g of Cu per plant) and comparing two metal sources (CuSO4.5H2O or Cu(OH)2). Copper was mainly accumulated in roots with soil supply, but an increase of oxidative stress levels was observed in leaves. On the other hand, Cu concentrations were higher in leaves that received foliar sprays, mainly as Cu(OH)2. However, when sulfate was foliar sprayed, plants exhibited more symptoms of injuries in the canopy with decreased chlorophyll contents and increased hydrogen peroxide and lipid peroxidation levels. Copper toxicity was characterized by sap leakage from the trunk and twigs, which is the first report of this specific Cu excess symptom in woody trees. Despite plants with 8.0 g of Cu soil-applied exhibiting the sap leakage, growth of new plant parts was more vigorous with lower oxidative stress levels and injuries compared to those with 4.0 g of Cu soil-applied (without sap leakage). With the highest level of Cu applied via foliar as sulfate, Cu was eliminated by plant roots, increasing the rhizospheric soil metal levels. Despite citrus likely exhibiting different mechanisms to reduce the damages caused by metal toxicity, such as responsive enzymatic antioxidant system, metal accumulation in the roots, and metal exclusion by roots, excess Cu resulted in damages on plant growth and metabolism when the metal was taken up either by roots or leaves.},

keywords = {Atmospheric Protection/Air Quality Control/Air Pollution, Ecotoxicology, Environment, Environmental Chemistry, Environmental Health, general, Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds},

author = {Aliandra G. Medeiros and Daiani C. Savi and Prithiba Mitra and Khaled A. Shaaban and Amit K. Jha and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-0587-2},

doi = {10.1007/S12223-018-0587-2},

issn = {1874-9356},

year  = {2018},

date = {2018-01-01},

journal = {Folia Microbiologica 2018 63:4},

volume = {63},

issue = {4},

pages = {499-505},

publisher = {Springer},

abstract = {Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds},

author = {Aliandra G. Medeiros and Daiani C. Savi and Prithiba Mitra and Khaled A. Shaaban and Amit K. Jha and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-0587-2},

doi = {10.1007/S12223-018-0587-2},

issn = {1874-9356},

year  = {2018},

date = {2018-01-01},

journal = {Folia Microbiologica 2018 63:4},

volume = {63},

issue = {4},

pages = {499-505},

publisher = {Springer},

abstract = {Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds},

author = {Aliandra G. Medeiros and Daiani C. Savi and Prithiba Mitra and Khaled A. Shaaban and Amit K. Jha and Jon S. Thorson and Jürgen Rohr and Chirlei Glienke},

url = {https://link.springer.com/article/10.1007/s12223-018-0587-2},

doi = {10.1007/S12223-018-0587-2},

issn = {1874-9356},

year  = {2018},

date = {2018-01-01},

journal = {Folia Microbiologica 2018 63:4},

volume = {63},

issue = {4},

pages = {499-505},

publisher = {Springer},

abstract = {Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.},

keywords = {Applied Microbiology, Environmental Engineering/Biotechnology, general, Immunology, Life Sciences, Microbiology},

pubstate = {published},

tppubtype = {article}

}

@article{Hippler2018,

title = {Mechanisms of copper stress alleviation in Citrus trees after metal uptake by leaves or roots},

author = {Franz Walter Rieger Hippler and Guilherme Petená and Rodrigo Marcelli Boaretto and José Antônio Quaggio and Ricardo Antunes Azevedo and Dirceu Mattos-Jr},

url = {https://link.springer.com/article/10.1007/s11356-018-1529-x},

doi = {10.1007/S11356-018-1529-X},

issn = {1614-7499},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research 2018 25:13},

volume = {25},

issue = {13},

pages = {13134-13146},

publisher = {Springer},

abstract = {Nutritional disorders caused by copper (Cu) have affected citrus orchards. Since Cu is foliar sprayed as a pesticide to control citrus diseases, this metal accumulates in the soil. Thereby, we evaluated the effects of Cu leaf absorption after spray of different metal sources, as well as roots absorption on growth, nutritional status, and oxidative stress of young sweet orange trees. Two experiments were carried out under greenhouse conditions. The first experiment was set up with varying Cu levels to the soil (nil Cu, 0.5, 2.0, 4.0 and 8.0 g of Cu per plant as CuSO4.5H2O), whereas the second experiment with Cu application via foliar sprays (0.5 and 2.0 g of Cu per plant) and comparing two metal sources (CuSO4.5H2O or Cu(OH)2). Copper was mainly accumulated in roots with soil supply, but an increase of oxidative stress levels was observed in leaves. On the other hand, Cu concentrations were higher in leaves that received foliar sprays, mainly as Cu(OH)2. However, when sulfate was foliar sprayed, plants exhibited more symptoms of injuries in the canopy with decreased chlorophyll contents and increased hydrogen peroxide and lipid peroxidation levels. Copper toxicity was characterized by sap leakage from the trunk and twigs, which is the first report of this specific Cu excess symptom in woody trees. Despite plants with 8.0 g of Cu soil-applied exhibiting the sap leakage, growth of new plant parts was more vigorous with lower oxidative stress levels and injuries compared to those with 4.0 g of Cu soil-applied (without sap leakage). With the highest level of Cu applied via foliar as sulfate, Cu was eliminated by plant roots, increasing the rhizospheric soil metal levels. Despite citrus likely exhibiting different mechanisms to reduce the damages caused by metal toxicity, such as responsive enzymatic antioxidant system, metal accumulation in the roots, and metal exclusion by roots, excess Cu resulted in damages on plant growth and metabolism when the metal was taken up either by roots or leaves.},

keywords = {Atmospheric Protection/Air Quality Control/Air Pollution, Ecotoxicology, Environment, Environmental Chemistry, Environmental Health, general, Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution},

pubstate = {published},

tppubtype = {article}

}

@article{Savietto2018,

title = {Antibacterial activity of monoacetylated alkyl gallates against Xanthomonas citri subsp. citri},

author = {Abigail Savietto and Carlos Roberto Polaquini and Malgorzata Kopacz and Dirk Jan Scheffers and Beatriz Carvalho Marques and Luís Octavio Regasini and Henrique Ferreira},

url = {https://link.springer.com/article/10.1007/s00203-018-1502-6},

doi = {10.1007/S00203-018-1502-6},

issn = {1432-072X},

year  = {2018},

date = {2018-01-01},

journal = {Archives of Microbiology 2018 200:6},

volume = {200},

issue = {6},

pages = {929-937},

publisher = {Springer},

abstract = {Asiatic citrus canker (ACC) is an incurable disease of citrus plants caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (X. citri). It affects all the commercially important citrus varieties in the major orange producing areas around the world. Control of the pathogen requires recurrent sprays of copper formulations that accumulate in soil and water reservoirs. Here, we describe the improvement of the alkyl gallates, which are potent anti-X. citri compounds, intended to be used as alternatives to copper in the control of ACC. Acetylation of alkyl gallates increased their lipophilicity, which resulted in potentiation of the antibacterial activity. X. citri exposed to the acetylated compounds exhibited increased cell length that is consistent with the disruption of the cell division apparatus. Finally, we show that inhibition of cell division is an indirect effect that seemed to be caused by membrane permeabilization, which is apparently the primary target of the acetylated alkyl gallates.},

keywords = {Biochemistry, Biotechnology, Cell Biology, Ecology, general, Microbial Ecology, Microbiology},

pubstate = {published},

tppubtype = {article}

}