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2020
Esteves, Mariana Bossi; Nalin, Julia Lopes; Kudlawiec, Karla; Salviatto, Raquel Caserta; Sales, Tiago Melo; Sicard, Anne; Almeida, Rodrigo Piacentini Paes; Souza, Alessandra Alves; Lopes, João Roberto Spotti
XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca Journal Article
In: Insects 2020, Vol. 11, Page 473, vol. 11, iss. 8, pp. 473, 2020, ISSN: 2075-4450.
Abstract | Links | BibTeX | Tags: attachment, bacterium, blocking transmission, borne, sharpshooter, vector
@article{Esteves2020,
title = {XadA2 Adhesin Decreases Biofilm Formation and Transmission of Xylella fastidiosa subsp. pauca},
author = {Mariana Bossi Esteves and Julia Lopes Nalin and Karla Kudlawiec and Raquel Caserta Salviatto and Tiago Melo Sales and Anne Sicard and Rodrigo Piacentini Paes Almeida and Alessandra Alves Souza and João Roberto Spotti Lopes},
url = {https://www.mdpi.com/2075-4450/11/8/473/htm https://www.mdpi.com/2075-4450/11/8/473},
doi = {10.3390/INSECTS11080473},
issn = {2075-4450},
year = {2020},
date = {2020-01-01},
journal = {Insects 2020, Vol. 11, Page 473},
volume = {11},
issue = {8},
pages = {473},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Xylella fastidiosa is a vector-borne bacterium that causes diseases in many plants of economic interest. The bacterium-vector initial interactions involve bacterial membrane-bound adhesins that mediate cell attachment to the foregut of insect vectors. We investigated the role of the afimbrial adhesin XadA2 in the binding and biofilm formation of X. fastidiosa subsp. pauca to vector surfaces in vitro, as well as its potential to disrupt pathogen transmission. We showed that XadA2 has binding affinity for polysaccharides on sharpshooter hindwings, used as a proxy for the interactions between X. fastidiosa and vectors. When in a medium without carbon sources, the bacterium used wing components, likely chitin, as a source of nutrients and formed a biofilm on the wing surface. There was a significant reduction in X. fastidiosa biofilm formation and cell aggregation on vector wings in competition assays with XadA2 or its specific antibody (anti-XadA2). Finally, pathogen acquisition and transmission to plant were significantly reduced when the vectors acquired X. fastidiosa from an artificial diet supplemented with anti-XadA2. These results show that XadA2 is important in mediating bacterial colonization in the insect and that it could be used as a target for blocking X. fastidiosa transmission.},
keywords = {attachment, bacterium, blocking transmission, borne, sharpshooter, vector},
pubstate = {published},
tppubtype = {article}
}
Xylella fastidiosa is a vector-borne bacterium that causes diseases in many plants of economic interest. The bacterium–vector initial interactions involve bacterial membrane-bound adhesins that mediate cell attachment to the foregut of insect vectors. We investigated the role of the afimbrial adhesin XadA2 in the binding and biofilm formation of X. fastidiosa subsp. pauca to vector surfaces in vitro, as well as its potential to disrupt pathogen transmission. We showed that XadA2 has binding affinity for polysaccharides on sharpshooter hindwings, used as a proxy for the interactions between X. fastidiosa and vectors. When in a medium without carbon sources, the bacterium used wing components, likely chitin, as a source of nutrients and formed a biofilm on the wing surface. There was a significant reduction in X. fastidiosa biofilm formation and cell aggregation on vector wings in competition assays with XadA2 or its specific antibody (anti-XadA2). Finally, pathogen acquisition and transmission to plant were significantly reduced when the vectors acquired X. fastidiosa from an artificial diet supplemented with anti-XadA2. These results show that XadA2 is important in mediating bacterial colonization in the insect and that it could be used as a target for blocking X. fastidiosa transmission.