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2020
Alencar, Luciano Delmondes; Azevedo, Patricia; Latado, Rodrigo Rocha
Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses Journal Article
In: Euphytica, vol. 216, iss. 2, pp. 1-11, 2020, ISSN: 15735060.
Abstract | Links | BibTeX | Tags: Chlorophyll index, Foliar anatomy, Parent-of-origin effects, Tomato, Triploidy
@article{nokey,
title = {Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses},
author = {Luciano Delmondes Alencar and Patricia Azevedo and Rodrigo Rocha Latado},
url = {https://link.springer.com/article/10.1007/s10681-020-2557-4},
doi = {10.1007/S10681-020-2557-4/METRICS},
issn = {15735060},
year = {2020},
date = {2020-01-01},
journal = {Euphytica},
volume = {216},
issue = {2},
pages = {1-11},
publisher = {Springer},
abstract = {Polyploidy is considered a valuable tool in plant breeding because can increase the size of flowers, leaves, and fruits or generate seedless plants, such as infertile triploids. Triploid plants can be obtained by reciprocal interploidy crosses (4x × 2x or 2x × 4x), but the influence of parent-of-origin effects in the plants’ phenotype caused by these interploidy crosses, as well as the interactions between cytoplasm and nucleus in these crosses are not clearly defined. Therefore, this work aimed to characterize the morphology and physiology of triploid tomato plants leaves by parent-of-origin 2x and 4x. For this purpose, we induct the tetraploidy with colchicine from diploid plants to performed the reciprocal interploidy crosses and obtained the triploid hybrids by differents parents-of-origin (2x or 4x). Histological slides from the leaves of all plant genotypes were assembled to analyze the anatomy, morphology and to measure the chlorophyll a, b, and the total index. Tetraploid tomato plants showed greater adaxial and total epidermis leaf thickness. Triploid plants by parent-of-origin 4x presented higher leaf thickness and spongy parenchyma than the triploid plants by parent-of-origin 2x, as well as higher rates of chlorophyll a and b, and an inbalance in the ratio of chlorophyll index a/b. We conclude that changes in cytoplasmatic organelles, caused by tetraploidization, can be transferred from parent-of-origin 4x to triploid plants. However, further studies are necessary, aiming to understand the genetic interaction between the cytoplasmatic genome (and consequently organelles) and the nucleus genome of triploid plants by differents parents-of-origin.},
keywords = {Chlorophyll index, Foliar anatomy, Parent-of-origin effects, Tomato, Triploidy},
pubstate = {published},
tppubtype = {article}
}
Polyploidy is considered a valuable tool in plant breeding because can increase the size of flowers, leaves, and fruits or generate seedless plants, such as infertile triploids. Triploid plants can be obtained by reciprocal interploidy crosses (4x × 2x or 2x × 4x), but the influence of parent-of-origin effects in the plants’ phenotype caused by these interploidy crosses, as well as the interactions between cytoplasm and nucleus in these crosses are not clearly defined. Therefore, this work aimed to characterize the morphology and physiology of triploid tomato plants leaves by parent-of-origin 2x and 4x. For this purpose, we induct the tetraploidy with colchicine from diploid plants to performed the reciprocal interploidy crosses and obtained the triploid hybrids by differents parents-of-origin (2x or 4x). Histological slides from the leaves of all plant genotypes were assembled to analyze the anatomy, morphology and to measure the chlorophyll a, b, and the total index. Tetraploid tomato plants showed greater adaxial and total epidermis leaf thickness. Triploid plants by parent-of-origin 4x presented higher leaf thickness and spongy parenchyma than the triploid plants by parent-of-origin 2x, as well as higher rates of chlorophyll a and b, and an inbalance in the ratio of chlorophyll index a/b. We conclude that changes in cytoplasmatic organelles, caused by tetraploidization, can be transferred from parent-of-origin 4x to triploid plants. However, further studies are necessary, aiming to understand the genetic interaction between the cytoplasmatic genome (and consequently organelles) and the nucleus genome of triploid plants by differents parents-of-origin.
Alencar, Luciano Delmondes; Azevedo, Patricia; Latado, Rodrigo Rocha
Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses Journal Article
In: Euphytica, vol. 216, iss. 2, pp. 1-11, 2020, ISSN: 15735060.
Abstract | Links | BibTeX | Tags: Chlorophyll index, Foliar anatomy, Parent-of-origin effects, Tomato, Triploidy
@article{nokey,
title = {Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses},
author = {Luciano Delmondes Alencar and Patricia Azevedo and Rodrigo Rocha Latado},
url = {https://link.springer.com/article/10.1007/s10681-020-2557-4},
doi = {10.1007/S10681-020-2557-4/METRICS},
issn = {15735060},
year = {2020},
date = {2020-01-01},
journal = {Euphytica},
volume = {216},
issue = {2},
pages = {1-11},
publisher = {Springer},
abstract = {Polyploidy is considered a valuable tool in plant breeding because can increase the size of flowers, leaves, and fruits or generate seedless plants, such as infertile triploids. Triploid plants can be obtained by reciprocal interploidy crosses (4x × 2x or 2x × 4x), but the influence of parent-of-origin effects in the plants’ phenotype caused by these interploidy crosses, as well as the interactions between cytoplasm and nucleus in these crosses are not clearly defined. Therefore, this work aimed to characterize the morphology and physiology of triploid tomato plants leaves by parent-of-origin 2x and 4x. For this purpose, we induct the tetraploidy with colchicine from diploid plants to performed the reciprocal interploidy crosses and obtained the triploid hybrids by differents parents-of-origin (2x or 4x). Histological slides from the leaves of all plant genotypes were assembled to analyze the anatomy, morphology and to measure the chlorophyll a, b, and the total index. Tetraploid tomato plants showed greater adaxial and total epidermis leaf thickness. Triploid plants by parent-of-origin 4x presented higher leaf thickness and spongy parenchyma than the triploid plants by parent-of-origin 2x, as well as higher rates of chlorophyll a and b, and an inbalance in the ratio of chlorophyll index a/b. We conclude that changes in cytoplasmatic organelles, caused by tetraploidization, can be transferred from parent-of-origin 4x to triploid plants. However, further studies are necessary, aiming to understand the genetic interaction between the cytoplasmatic genome (and consequently organelles) and the nucleus genome of triploid plants by differents parents-of-origin.},
keywords = {Chlorophyll index, Foliar anatomy, Parent-of-origin effects, Tomato, Triploidy},
pubstate = {published},
tppubtype = {article}
}
Polyploidy is considered a valuable tool in plant breeding because can increase the size of flowers, leaves, and fruits or generate seedless plants, such as infertile triploids. Triploid plants can be obtained by reciprocal interploidy crosses (4x × 2x or 2x × 4x), but the influence of parent-of-origin effects in the plants’ phenotype caused by these interploidy crosses, as well as the interactions between cytoplasm and nucleus in these crosses are not clearly defined. Therefore, this work aimed to characterize the morphology and physiology of triploid tomato plants leaves by parent-of-origin 2x and 4x. For this purpose, we induct the tetraploidy with colchicine from diploid plants to performed the reciprocal interploidy crosses and obtained the triploid hybrids by differents parents-of-origin (2x or 4x). Histological slides from the leaves of all plant genotypes were assembled to analyze the anatomy, morphology and to measure the chlorophyll a, b, and the total index. Tetraploid tomato plants showed greater adaxial and total epidermis leaf thickness. Triploid plants by parent-of-origin 4x presented higher leaf thickness and spongy parenchyma than the triploid plants by parent-of-origin 2x, as well as higher rates of chlorophyll a and b, and an inbalance in the ratio of chlorophyll index a/b. We conclude that changes in cytoplasmatic organelles, caused by tetraploidization, can be transferred from parent-of-origin 4x to triploid plants. However, further studies are necessary, aiming to understand the genetic interaction between the cytoplasmatic genome (and consequently organelles) and the nucleus genome of triploid plants by differents parents-of-origin.
2019
Pinheiro, Thaísa T.; Peres, Lázaro E. P.; Purgatto, Eduardo; Latado, Rodrigo R.; Maniero, Rodolfo A.; Martins, Mônica M.; Figueira, Antonio
Citrus carotenoid isomerase gene characterization by complementation of the “Micro-Tom” tangerine mutant Journal Article
In: Plant Cell Reports, vol. 38, iss. 5, pp. 623-636, 2019, ISSN: 07217714.
Abstract | Links | BibTeX | Tags: Carotenoid biosynthesis, CRTISO, Functional genomics, sweet orange, Tomato
@article{Pinheiro2019,
title = {Citrus carotenoid isomerase gene characterization by complementation of the “Micro-Tom” tangerine mutant},
author = {Thaísa T. Pinheiro and Lázaro E. P. Peres and Eduardo Purgatto and Rodrigo R. Latado and Rodolfo A. Maniero and Mônica M. Martins and Antonio Figueira},
url = {https://link.springer.com/article/10.1007/s00299-019-02393-2},
doi = {10.1007/S00299-019-02393-2/METRICS},
issn = {07217714},
year = {2019},
date = {2019-01-01},
journal = {Plant Cell Reports},
volume = {38},
issue = {5},
pages = {623-636},
publisher = {Springer Verlag},
abstract = {Key message: Complementation of the “Micro-Tom” tomato tangerine mutant with a Citrus CRTISO allele restores the wild-type fruit carotenoid profile, indicating that the Citrus allele encodes an authentic functional carotenoid isomerase. Abstract: Citrus fruits are rich in carotenoids; the genus offers a large diversity in composition, yet to be fully explored to improve fruit nutritional quality. As perennial tree species, Citrus lack the resources for functional genetic studies, requiring the use of model plant systems. Here, we used the “Micro-Tom” (MT) tomato carrying the tangerine mutation (t), deficient for the carotenoid isomerase (CRTISO) gene, to functionally characterize the homologous C. sinensis genes. We identified four putative loci in the C. sinensis genome, named CsCRTISO, CsCRTISO-Like 1, CsCRTISO-Like 2, and CsCRTISO-Like 2B, with the latter as a presumed duplication of CRTISO-Like 2. In general, all the Citrus paralogs showed less expression specialization than the tomato ones, with CsCRTISO being the most expressed gene in all tissues analyzed. MT-t plants were successfully complemented with the CsCRTISO, and fruits showed a carotenoid profile similar to the control, indicating that the Citrus allele indeed encodes an authentic functional carotenoid isomerase and that the signal peptide is functional in tomato. MT was silenced using an inverted repeat of a fragment from the Citrus CRTISO resulting in a stronger phenotype than MT-t. MT-t and MT silenced for CRTISO presented an overall decrease in transcript accumulation of all genes from the biosynthesis pathway. The expression of the Citrus CRTISO gene is able to restore the biosynthesis of carotenoids with the appropriate regulation in MT-t. The decrease in transcript accumulation in MT-t and MT-CRTISO-suppressed lines reinforces previous suggestions that transcriptional regulation of the carotenoid biosynthesis involves regulatory loops by intermediate products.},
keywords = {Carotenoid biosynthesis, CRTISO, Functional genomics, sweet orange, Tomato},
pubstate = {published},
tppubtype = {article}
}
Key message: Complementation of the “Micro-Tom” tomato tangerine mutant with a Citrus CRTISO allele restores the wild-type fruit carotenoid profile, indicating that the Citrus allele encodes an authentic functional carotenoid isomerase. Abstract: Citrus fruits are rich in carotenoids; the genus offers a large diversity in composition, yet to be fully explored to improve fruit nutritional quality. As perennial tree species, Citrus lack the resources for functional genetic studies, requiring the use of model plant systems. Here, we used the “Micro-Tom” (MT) tomato carrying the tangerine mutation (t), deficient for the carotenoid isomerase (CRTISO) gene, to functionally characterize the homologous C. sinensis genes. We identified four putative loci in the C. sinensis genome, named CsCRTISO, CsCRTISO-Like 1, CsCRTISO-Like 2, and CsCRTISO-Like 2B, with the latter as a presumed duplication of CRTISO-Like 2. In general, all the Citrus paralogs showed less expression specialization than the tomato ones, with CsCRTISO being the most expressed gene in all tissues analyzed. MT-t plants were successfully complemented with the CsCRTISO, and fruits showed a carotenoid profile similar to the control, indicating that the Citrus allele indeed encodes an authentic functional carotenoid isomerase and that the signal peptide is functional in tomato. MT was silenced using an inverted repeat of a fragment from the Citrus CRTISO resulting in a stronger phenotype than MT-t. MT-t and MT silenced for CRTISO presented an overall decrease in transcript accumulation of all genes from the biosynthesis pathway. The expression of the Citrus CRTISO gene is able to restore the biosynthesis of carotenoids with the appropriate regulation in MT-t. The decrease in transcript accumulation in MT-t and MT-CRTISO-suppressed lines reinforces previous suggestions that transcriptional regulation of the carotenoid biosynthesis involves regulatory loops by intermediate products.
2018
Purić, J.; Vieira, G.; Cavalca, L. B.; Sette, L. D.; Ferreira, H.; Vieira, M. L. C.; Sass, D. C.
Activity of Antarctic fungi extracts against phytopathogenic bacteria Journal Article
In: Letters in Applied Microbiology, vol. 66, iss. 6, pp. 530-536, 2018, ISSN: 1472-765X.
Abstract | Links | BibTeX | Tags: Antibacterial, passionfruit, Phytopathogen, Tomato, Xanthomonas
@article{nokey,
title = {Activity of Antarctic fungi extracts against phytopathogenic bacteria},
author = {J. Purić and G. Vieira and L. B. Cavalca and L. D. Sette and H. Ferreira and M. L. C. Vieira and D. C. Sass},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/lam.12875 https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.12875 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/lam.12875},
doi = {10.1111/LAM.12875},
issn = {1472-765X},
year = {2018},
date = {2018-01-01},
journal = {Letters in Applied Microbiology},
volume = {66},
issue = {6},
pages = {530-536},
publisher = {John Wiley & Sons, Ltd},
abstract = {This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctic ecosystems and to assess its potential antibacterial activity on Xanthomonas euvesicatoria and Xanthomonas axonopodis pv. passiflorae (phytopathogenic bacteria causing diseases in pepper and tomato and passionfruit, respectively). Among the 66 crude intracellular and extracellular extracts obtained from fungi recovered from soil and 79 obtained from marine sediment samples, 25 showed the ability to prevent the growth of X. euvesicatoria in vitro and 28 showed the ability to prevent the growth of X. axonopodis pv. passiflorae in vitro. Intracellular and extracellular extracts from soil fungi inhibited around 97% of X. euvesicatoria and 98% of X. axonopodis pv. passiflorae at 2·1 mg ml−1. The average inhibition rates against X. euvesicatoria and X. axonopodis pv. passiflorae for intracellular and extracellular extracts from marine sediments fungi were around 96 and 97%, respectively, at 3·0 mg ml−1. Extracts containing secondary metabolites with antimicrobial activity against X. euvesicatoria and X. axonopodis pv. passiflorae were obtained, containing possible substitutes for the products currently used to control these phytopathogens. Significance and Impact of the Study: Micro-organisms from extreme ecosystems, such as the Antarctic ecosystem, need to survive in harsh conditions with low temperatures, low nutrients and high UV radiation. Micro-organisms adapt to these conditions evolving diverse biochemical and physiological adaptations essential for survival. All this makes these micro-organisms a rich source of novel natural products based on unique chemical scaffolds. Discovering novel bioactive compounds is essential because of the rise in antibiotic-resistant micro-organisms and the emergence of new infections. Fungi from Antarctic environments have been proven to produce bioactive secondary metabolites against various micro-organisms, but few studies have shown activity against Xanthomonas phytopathogens.},
keywords = {Antibacterial, passionfruit, Phytopathogen, Tomato, Xanthomonas},
pubstate = {published},
tppubtype = {article}
}
This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctic ecosystems and to assess its potential antibacterial activity on Xanthomonas euvesicatoria and Xanthomonas axonopodis pv. passiflorae (phytopathogenic bacteria causing diseases in pepper and tomato and passionfruit, respectively). Among the 66 crude intracellular and extracellular extracts obtained from fungi recovered from soil and 79 obtained from marine sediment samples, 25 showed the ability to prevent the growth of X. euvesicatoria in vitro and 28 showed the ability to prevent the growth of X. axonopodis pv. passiflorae in vitro. Intracellular and extracellular extracts from soil fungi inhibited around 97% of X. euvesicatoria and 98% of X. axonopodis pv. passiflorae at 2·1 mg ml−1. The average inhibition rates against X. euvesicatoria and X. axonopodis pv. passiflorae for intracellular and extracellular extracts from marine sediments fungi were around 96 and 97%, respectively, at 3·0 mg ml−1. Extracts containing secondary metabolites with antimicrobial activity against X. euvesicatoria and X. axonopodis pv. passiflorae were obtained, containing possible substitutes for the products currently used to control these phytopathogens. Significance and Impact of the Study: Micro-organisms from extreme ecosystems, such as the Antarctic ecosystem, need to survive in harsh conditions with low temperatures, low nutrients and high UV radiation. Micro-organisms adapt to these conditions evolving diverse biochemical and physiological adaptations essential for survival. All this makes these micro-organisms a rich source of novel natural products based on unique chemical scaffolds. Discovering novel bioactive compounds is essential because of the rise in antibiotic-resistant micro-organisms and the emergence of new infections. Fungi from Antarctic environments have been proven to produce bioactive secondary metabolites against various micro-organisms, but few studies have shown activity against Xanthomonas phytopathogens.
Purić, J.; Vieira, G.; Cavalca, L. B.; Sette, L. D.; Ferreira, H.; Vieira, M. L. C.; Sass, D. C.
Activity of Antarctic fungi extracts against phytopathogenic bacteria Journal Article
In: Letters in Applied Microbiology, vol. 66, iss. 6, pp. 530-536, 2018, ISSN: 1472-765X.
Abstract | Links | BibTeX | Tags: Antibacterial, passionfruit, Phytopathogen, Tomato, Xanthomonas
@article{nokey,
title = {Activity of Antarctic fungi extracts against phytopathogenic bacteria},
author = {J. Purić and G. Vieira and L. B. Cavalca and L. D. Sette and H. Ferreira and M. L. C. Vieira and D. C. Sass},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/lam.12875 https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.12875 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/lam.12875},
doi = {10.1111/LAM.12875},
issn = {1472-765X},
year = {2018},
date = {2018-01-01},
journal = {Letters in Applied Microbiology},
volume = {66},
issue = {6},
pages = {530-536},
publisher = {John Wiley & Sons, Ltd},
abstract = {This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctic ecosystems and to assess its potential antibacterial activity on Xanthomonas euvesicatoria and Xanthomonas axonopodis pv. passiflorae (phytopathogenic bacteria causing diseases in pepper and tomato and passionfruit, respectively). Among the 66 crude intracellular and extracellular extracts obtained from fungi recovered from soil and 79 obtained from marine sediment samples, 25 showed the ability to prevent the growth of X. euvesicatoria in vitro and 28 showed the ability to prevent the growth of X. axonopodis pv. passiflorae in vitro. Intracellular and extracellular extracts from soil fungi inhibited around 97% of X. euvesicatoria and 98% of X. axonopodis pv. passiflorae at 2·1 mg ml−1. The average inhibition rates against X. euvesicatoria and X. axonopodis pv. passiflorae for intracellular and extracellular extracts from marine sediments fungi were around 96 and 97%, respectively, at 3·0 mg ml−1. Extracts containing secondary metabolites with antimicrobial activity against X. euvesicatoria and X. axonopodis pv. passiflorae were obtained, containing possible substitutes for the products currently used to control these phytopathogens. Significance and Impact of the Study: Micro-organisms from extreme ecosystems, such as the Antarctic ecosystem, need to survive in harsh conditions with low temperatures, low nutrients and high UV radiation. Micro-organisms adapt to these conditions evolving diverse biochemical and physiological adaptations essential for survival. All this makes these micro-organisms a rich source of novel natural products based on unique chemical scaffolds. Discovering novel bioactive compounds is essential because of the rise in antibiotic-resistant micro-organisms and the emergence of new infections. Fungi from Antarctic environments have been proven to produce bioactive secondary metabolites against various micro-organisms, but few studies have shown activity against Xanthomonas phytopathogens.},
keywords = {Antibacterial, passionfruit, Phytopathogen, Tomato, Xanthomonas},
pubstate = {published},
tppubtype = {article}
}
This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctic ecosystems and to assess its potential antibacterial activity on Xanthomonas euvesicatoria and Xanthomonas axonopodis pv. passiflorae (phytopathogenic bacteria causing diseases in pepper and tomato and passionfruit, respectively). Among the 66 crude intracellular and extracellular extracts obtained from fungi recovered from soil and 79 obtained from marine sediment samples, 25 showed the ability to prevent the growth of X. euvesicatoria in vitro and 28 showed the ability to prevent the growth of X. axonopodis pv. passiflorae in vitro. Intracellular and extracellular extracts from soil fungi inhibited around 97% of X. euvesicatoria and 98% of X. axonopodis pv. passiflorae at 2·1 mg ml−1. The average inhibition rates against X. euvesicatoria and X. axonopodis pv. passiflorae for intracellular and extracellular extracts from marine sediments fungi were around 96 and 97%, respectively, at 3·0 mg ml−1. Extracts containing secondary metabolites with antimicrobial activity against X. euvesicatoria and X. axonopodis pv. passiflorae were obtained, containing possible substitutes for the products currently used to control these phytopathogens. Significance and Impact of the Study: Micro-organisms from extreme ecosystems, such as the Antarctic ecosystem, need to survive in harsh conditions with low temperatures, low nutrients and high UV radiation. Micro-organisms adapt to these conditions evolving diverse biochemical and physiological adaptations essential for survival. All this makes these micro-organisms a rich source of novel natural products based on unique chemical scaffolds. Discovering novel bioactive compounds is essential because of the rise in antibiotic-resistant micro-organisms and the emergence of new infections. Fungi from Antarctic environments have been proven to produce bioactive secondary metabolites against various micro-organisms, but few studies have shown activity against Xanthomonas phytopathogens.