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2022
Sinico, Thaís Elise; Nunes, Maria Andréia; Kitajima, Elliot Watanabe; Cunha, Bruna Aparecida; Novelli, Valdenice Moreira
Notes on the embryological development of the Brevipalpus yothersi (Acari: Tenuipalpidae) Journal Article
In: Acarologia, vol. 62, iss. 1, pp. 113-119, 2022, ISSN: 0044-586X.
Abstract | Links | BibTeX | Tags: Acari, Acariformes, Animalia, Arachnida, Arthropoda, citrus leprosis disease, embryology, mite, phytophagous mite, vector
@article{Sinico2022,
title = {Notes on the embryological development of the Brevipalpus yothersi (Acari: Tenuipalpidae)},
author = {Thaís Elise Sinico and Maria Andréia Nunes and Elliot Watanabe Kitajima and Bruna Aparecida Cunha and Valdenice Moreira Novelli},
url = {https://www1.montpellier.inra.fr/CBGP/acarologia/article.php?id=4496},
doi = {10.24349/0GR3-3A6B},
issn = {0044-586X},
year = {2022},
date = {2022-01-01},
journal = {Acarologia},
volume = {62},
issue = {1},
pages = {113-119},
publisher = {Les Amis d'Acarologia},
abstract = {Mites of the Brevipalpus genus are economically important due to their role as vectors of plant viruses. Among them, B. yothersi is well studied because it is the recognized main vector of the citrus leprosis virus C (CiLV-C), the causal agent of citrus leprosis, which is one of the most damaging viral diseases in the citrus crops. However, studies of several aspects of the biology of B. yothersi are still scarce, including its embryology. We performed a descriptive embryonic development study of B. yothersi, utilizing light and scanning electron microscope to obtain biological information of this particular phytovirus vector. Distinct events were recorded, such as centering of the nucleus, mitotic divisions for primitive body formation and appendages, as well as the presence of channels inside the egg and their possible association with the respiratory tract. Here we provide the first notes about embryonic development of this important mite vector in the Americas.},
keywords = {Acari, Acariformes, Animalia, Arachnida, Arthropoda, citrus leprosis disease, embryology, mite, phytophagous mite, vector},
pubstate = {published},
tppubtype = {article}
}
Mites of the Brevipalpus genus are economically important due to their role as vectors of plant viruses. Among them, B. yothersi is well studied because it is the recognized main vector of the citrus leprosis virus C (CiLV-C), the causal agent of citrus leprosis, which is one of the most damaging viral diseases in the citrus crops. However, studies of several aspects of the biology of B. yothersi are still scarce, including its embryology. We performed a descriptive embryonic development study of B. yothersi, utilizing light and scanning electron microscope to obtain biological information of this particular phytovirus vector. Distinct events were recorded, such as centering of the nucleus, mitotic divisions for primitive body formation and appendages, as well as the presence of channels inside the egg and their possible association with the respiratory tract. Here we provide the first notes about embryonic development of this important mite vector in the Americas.
2021
Chabi-Jesus, Camila; Ramos-González, Pedro L.; Postclam-Barro, Matheus; Fontenele, Rafaela Salgado; Harakava, Ricardo; Bassanezi, Renato B.; Moreira, Alecio S.; Kitajima, Elliot W.; Varsani, Arvind; Freitas-Astúa, Juliana
Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas Journal Article
In: Frontiers in Microbiology, vol. 12, pp. 986, 2021, ISSN: 1664302X.
Abstract | Links | BibTeX | Tags: Brevipalpus mites, Cilevirus, citrus leprosis disease, Kitaviridae, virus evolution
@article{nokey,
title = {Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas},
author = {Camila Chabi-Jesus and Pedro L. Ramos-González and Matheus Postclam-Barro and Rafaela Salgado Fontenele and Ricardo Harakava and Renato B. Bassanezi and Alecio S. Moreira and Elliot W. Kitajima and Arvind Varsani and Juliana Freitas-Astúa},
doi = {10.3389/FMICB.2021.641252/BIBTEX},
issn = {1664302X},
year = {2021},
date = {2021-01-01},
journal = {Frontiers in Microbiology},
volume = {12},
pages = {986},
publisher = {Frontiers Media S.A.},
abstract = {Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region.},
keywords = {Brevipalpus mites, Cilevirus, citrus leprosis disease, Kitaviridae, virus evolution},
pubstate = {published},
tppubtype = {article}
}
Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region.
Chabi-Jesus, Camila; Ramos-González, Pedro L.; Postclam-Barro, Matheus; Fontenele, Rafaela Salgado; Harakava, Ricardo; Bassanezi, Renato B.; Moreira, Alecio S.; Kitajima, Elliot W.; Varsani, Arvind; Freitas-Astúa, Juliana
Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas Journal Article
In: Frontiers in Microbiology, vol. 12, pp. 986, 2021, ISSN: 1664302X.
Abstract | Links | BibTeX | Tags: Brevipalpus mites, Cilevirus, citrus leprosis disease, Kitaviridae, virus evolution
@article{nokey,
title = {Molecular Epidemiology of Citrus Leprosis Virus C: A New Viral Lineage and Phylodynamic of the Main Viral Subpopulations in the Americas},
author = {Camila Chabi-Jesus and Pedro L. Ramos-González and Matheus Postclam-Barro and Rafaela Salgado Fontenele and Ricardo Harakava and Renato B. Bassanezi and Alecio S. Moreira and Elliot W. Kitajima and Arvind Varsani and Juliana Freitas-Astúa},
doi = {10.3389/FMICB.2021.641252/BIBTEX},
issn = {1664302X},
year = {2021},
date = {2021-01-01},
journal = {Frontiers in Microbiology},
volume = {12},
pages = {986},
publisher = {Frontiers Media S.A.},
abstract = {Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region.},
keywords = {Brevipalpus mites, Cilevirus, citrus leprosis disease, Kitaviridae, virus evolution},
pubstate = {published},
tppubtype = {article}
}
Despite the importance of viral strains/variants as agents of emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed the population and spatial dynamic parameters of citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae). CiLV-C is the etiological agent of citrus leprosis disease, a non-systemic infection considered the main viral disorder affecting citrus orchards in Brazil. Overall, we obtained 18 complete or near-complete viral genomes, 123 complete nucleotide sequences of the open reading frame (ORF) encoding the putative coat protein, and 204 partial nucleotide sequences of the ORF encoding the movement protein, from 430 infected Citrus spp. samples collected between 1932 and 2020. A thorough examination of the collected dataset suggested that the CiLV-C population consists of the major lineages CRD and SJP, unevenly distributed, plus a third one called ASU identified in this work, which is represented by a single isolate found in an herbarium sample collected in Asuncion, Paraguay, in 1937. Viruses from the three lineages share about 85% nucleotide sequence identity and show signs of inter-clade recombination events. Members of the lineage CRD were identified both in commercial and non-commercial citrus orchards. However, those of the lineages SJP were exclusively detected in samples collected in the citrus belt of São Paulo and Minas Gerais, the leading Brazilian citrus production region, after 2015. The most recent common ancestor of viruses of the three lineages dates back to, at least, ∼1500 years ago. Since citrus plants were introduced in the Americas by the Portuguese around the 1520s, the Bayesian phylodynamic analysis suggested that the ancestors of the main CiLV-C lineages likely originated in contact with native vegetation of South America. The intensive expansion of CRD and SJP lineages in Brazil started probably linked to the beginning of the local citrus industry. The high prevalence of CiLV-C in the citrus belt of Brazil likely ensues from the intensive connectivity between orchards, which represents a potential risk toward pathogen saturation across the region.
2020
Ferreira, Leticia M.; Nunes, Maria A.; Sinico, Thaís E.; Soares, Alex J.; Novelli, Valdenice M.
Brevipalpus Species Vectoring Citrus Leprosis Virus (Cilevirus and Dichorhavirus) Journal Article
In: Journal of Economic Entomology, vol. 113, iss. 4, pp. 1628-1634, 2020, ISSN: 0022-0493.
Abstract | Links | BibTeX | Tags: citriculture, citrus leprosis disease, false spider mites, pest management
@article{Ferreira2020,
title = {Brevipalpus Species Vectoring Citrus Leprosis Virus (Cilevirus and Dichorhavirus)},
author = {Leticia M. Ferreira and Maria A. Nunes and Thaís E. Sinico and Alex J. Soares and Valdenice M. Novelli},
url = {https://academic.oup.com/jee/article/113/4/1628/5824968},
doi = {10.1093/JEE/TOAA070},
issn = {0022-0493},
year = {2020},
date = {2020-01-01},
journal = {Journal of Economic Entomology},
volume = {113},
issue = {4},
pages = {1628-1634},
publisher = {Oxford Academic},
abstract = {Citrus leprosis (CL) is one of the most devastating viral diseases of orchards, and industries correspondingly invest highly in the management and control of the virus vector. In Brazil, the disease is caused most predominantly by the citrus leprosis virus C (CiLV-C, Kitaviridae: Cilevirus), and also by citrus leprosis virus N (CiLV-N, Rhabdoviridae: Dichorhavirus). Both viruses are transmitted by false spider mites and at least three different species, Brevipalpus yothersi Baker, B. papayensis Baker, and B. phoenicis (Geijskes) sensu stricto, have been reported in citrus orchards. The main goal of this study was to evaluate the capacity of three Brevipalpus species to transmit citrus leprosis virus (cytoplasmic and nuclear types). The capacity of false spider mites to acquire the virus was accomplished using RT-PCR and the ability to inoculation the virus to host plants (common bean and sweet orange) was assessed via viral transmission assays. Common beans infested with B. yothersi and B. papayensis showed symptoms of CiLV-C in 87.5 and 17% of the plants assessed, respectively. In sweet orange, B. yothersi was exclusively able to inoculate CiLV-C, and around 83% of samples were symptomatic. Host plants infected with CiLV-N showed symptoms only when infested with B. phoenicis sensu stricto (s.s.). All the Brevipalpus species (Acari: Tenuipalpidae) were able to acquire both viruses (CiLV-C and CiLV-N), but not infect plants. These results suggest the existence of virus-vector specificity in the leprosis pathosystem, and this information will be critical for enhancing our further understanding of epidemiological features and disease management.},
keywords = {citriculture, citrus leprosis disease, false spider mites, pest management},
pubstate = {published},
tppubtype = {article}
}
Citrus leprosis (CL) is one of the most devastating viral diseases of orchards, and industries correspondingly invest highly in the management and control of the virus vector. In Brazil, the disease is caused most predominantly by the citrus leprosis virus C (CiLV-C, Kitaviridae: Cilevirus), and also by citrus leprosis virus N (CiLV-N, Rhabdoviridae: Dichorhavirus). Both viruses are transmitted by false spider mites and at least three different species, Brevipalpus yothersi Baker, B. papayensis Baker, and B. phoenicis (Geijskes) sensu stricto, have been reported in citrus orchards. The main goal of this study was to evaluate the capacity of three Brevipalpus species to transmit citrus leprosis virus (cytoplasmic and nuclear types). The capacity of false spider mites to acquire the virus was accomplished using RT-PCR and the ability to inoculation the virus to host plants (common bean and sweet orange) was assessed via viral transmission assays. Common beans infested with B. yothersi and B. papayensis showed symptoms of CiLV-C in 87.5 and 17% of the plants assessed, respectively. In sweet orange, B. yothersi was exclusively able to inoculate CiLV-C, and around 83% of samples were symptomatic. Host plants infected with CiLV-N showed symptoms only when infested with B. phoenicis sensu stricto (s.s.). All the Brevipalpus species (Acari: Tenuipalpidae) were able to acquire both viruses (CiLV-C and CiLV-N), but not infect plants. These results suggest the existence of virus-vector specificity in the leprosis pathosystem, and this information will be critical for enhancing our further understanding of epidemiological features and disease management.