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2021
Caccalano, M. N.; Dilarri, G.; Zamuner, C. F. C.; Domingues, D. S.; Ferreira, H.
Hexanoic acid: a new potential substitute for copper-based agrochemicals against citrus canker Journal Article
In: Journal of Applied Microbiology, vol. 131, iss. 5, pp. 2488-2499, 2021, ISSN: 1365-2672.
Abstract | Links | BibTeX | Tags: cell division, Disease management, membrane permeabilization, organic acid, phytotoxicity, Xanthomonas citri
@article{Caccalano2021,
title = {Hexanoic acid: a new potential substitute for copper-based agrochemicals against citrus canker},
author = {M. N. Caccalano and G. Dilarri and C. F. C. Zamuner and D. S. Domingues and H. Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1111/jam.15125 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.15125 https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/jam.15125},
doi = {10.1111/JAM.15125},
issn = {1365-2672},
year = {2021},
date = {2021-01-01},
journal = {Journal of Applied Microbiology},
volume = {131},
issue = {5},
pages = {2488-2499},
publisher = {John Wiley & Sons, Ltd},
abstract = {Aims: The aim of the study is to evaluate hexanoic acid (HA) as an alternative to manage citrus canker. Methods and Results: The minimal growth inhibitory concentration of HA against Xanthomonas citri subsp. citri was determined at 2·15 mmol l−1 using a respiratory activity assay. Growth curves at different pH values showed that growth inhibition was not due to media acidification induced by HA. The germination rate and root elongation of Lactuca sativa seeds exposed to different concentrations of HA (varying from 0·86 to 5·16 mmol l−1) were assessed to screen for phytotoxicity. The acid exhibited low phytotoxicity for L. sativa at 1·29 and 2·58 mmol l−1. To evaluate the ability of HA to protect citrus against X. citri infection, leaves of Citrus sinensis were sprayed with the acid and subsequently challenged with X. citri. HA at 3·44 mmol l−1 was able to protect citrus against infection, showing a reduction of three orders of magnitude in the number of citrus canker lesions per cm2 when compared to the untreated negative control. Conclusion: HA is a potential alternative to copper for citrus canker management. Significance and Impact of the Study: HA inhibits X. citri growth, exhibits low phytotoxicity and is an alternative to copper for the protection of citrus plants against bacterial infection.},
keywords = {cell division, Disease management, membrane permeabilization, organic acid, phytotoxicity, Xanthomonas citri},
pubstate = {published},
tppubtype = {article}
}
Aims: The aim of the study is to evaluate hexanoic acid (HA) as an alternative to manage citrus canker. Methods and Results: The minimal growth inhibitory concentration of HA against Xanthomonas citri subsp. citri was determined at 2·15 mmol l−1 using a respiratory activity assay. Growth curves at different pH values showed that growth inhibition was not due to media acidification induced by HA. The germination rate and root elongation of Lactuca sativa seeds exposed to different concentrations of HA (varying from 0·86 to 5·16 mmol l−1) were assessed to screen for phytotoxicity. The acid exhibited low phytotoxicity for L. sativa at 1·29 and 2·58 mmol l−1. To evaluate the ability of HA to protect citrus against X. citri infection, leaves of Citrus sinensis were sprayed with the acid and subsequently challenged with X. citri. HA at 3·44 mmol l−1 was able to protect citrus against infection, showing a reduction of three orders of magnitude in the number of citrus canker lesions per cm2 when compared to the untreated negative control. Conclusion: HA is a potential alternative to copper for citrus canker management. Significance and Impact of the Study: HA inhibits X. citri growth, exhibits low phytotoxicity and is an alternative to copper for the protection of citrus plants against bacterial infection.
Dilarri, Guilherme; Zamuner, Caio F. C.; Mendes, Carolina R.; Junior, José R. M.; Morão, Luana G.; Montagnolli, Renato N.; Bidoia, Ederio D.; Ferreira, Henrique
Evaluating the potential of electrolysed water for the disinfection of citrus fruit in packinghouses Journal Article
In: Journal of the Science of Food and Agriculture, vol. 101, iss. 6, pp. 2584-2591, 2021, ISSN: 1097-0010.
Abstract | Links | BibTeX | Tags: electrolysed water, Lime, membrane permeabilization, sanitizer, Xanthomonas citri subsp. citri
@article{Dilarri2021,
title = {Evaluating the potential of electrolysed water for the disinfection of citrus fruit in packinghouses},
author = {Guilherme Dilarri and Caio F. C. Zamuner and Carolina R. Mendes and José R. M. Junior and Luana G. Morão and Renato N. Montagnolli and Ederio D. Bidoia and Henrique Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/jsfa.10888 https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.10888 https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10888},
doi = {10.1002/JSFA.10888},
issn = {1097-0010},
year = {2021},
date = {2021-01-01},
journal = {Journal of the Science of Food and Agriculture},
volume = {101},
issue = {6},
pages = {2584-2591},
publisher = {John Wiley & Sons, Ltd},
abstract = {BACKGROUND: The largest and most profitable market for citrus is the production of fresh fruit. Xanthomonas citri subsp. citri is a Gram-negative plant pathogen and the etiological agent of citrus canker, one of the major threats to citrus production worldwide. In the early stages of infection, X. citri can attach to plant surfaces by means of biofilms. Biofilm is considered an essential virulence factor, which helps tissue colonization in plants. Thus, sanitization of citrus fruit is mandatory in packinghouses before any logistic operation as packing and shipment to the market. The aim of this study was to evaluate electrolysed water (EW) as a sanitizer for the disinfection of citrus fruit in packinghouses. RESULTS: Using a protocol to monitor cell respiration we show that EW, obtained after 8 and 9 min of electrolysis, sufficed to kill X. citri when applied at a concentration of 500 μL mL−1. Furthermore, microscopy analysis, combined with time-response growth curves, confirmed that EW affects the bacterial cytoplasmatic membrane and it leads to cell death in the first few minutes of contact. Pathogenicity tests using limes to simulate packinghouse treatment showed that EW, produced with 9 min of electrolysis, was a very effective sanitizer capable of eliminating X. citri from contaminated fruit. CONCLUSION: It was possible to conclude that EW is significantly effective as sodium hypochlorite (NaClO) at 200 ppm. Therefore, EW could be an alternative for citrus sanitization in packinghouses. © 2020 Society of Chemical Industry.},
keywords = {electrolysed water, Lime, membrane permeabilization, sanitizer, Xanthomonas citri subsp. citri},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: The largest and most profitable market for citrus is the production of fresh fruit. Xanthomonas citri subsp. citri is a Gram-negative plant pathogen and the etiological agent of citrus canker, one of the major threats to citrus production worldwide. In the early stages of infection, X. citri can attach to plant surfaces by means of biofilms. Biofilm is considered an essential virulence factor, which helps tissue colonization in plants. Thus, sanitization of citrus fruit is mandatory in packinghouses before any logistic operation as packing and shipment to the market. The aim of this study was to evaluate electrolysed water (EW) as a sanitizer for the disinfection of citrus fruit in packinghouses. RESULTS: Using a protocol to monitor cell respiration we show that EW, obtained after 8 and 9 min of electrolysis, sufficed to kill X. citri when applied at a concentration of 500 μL mL−1. Furthermore, microscopy analysis, combined with time-response growth curves, confirmed that EW affects the bacterial cytoplasmatic membrane and it leads to cell death in the first few minutes of contact. Pathogenicity tests using limes to simulate packinghouse treatment showed that EW, produced with 9 min of electrolysis, was a very effective sanitizer capable of eliminating X. citri from contaminated fruit. CONCLUSION: It was possible to conclude that EW is significantly effective as sodium hypochlorite (NaClO) at 200 ppm. Therefore, EW could be an alternative for citrus sanitization in packinghouses. © 2020 Society of Chemical Industry.
2019
Morão, Luana G.; Polaquini, Carlos R.; Kopacz, Malgorzata; Torrezan, Guilherme S.; Ayusso, Gabriela M.; Dilarri, Guilherme; Cavalca, Lúcia B.; Zielińska, Aleksandra; Scheffers, Dirk Jan; Regasini, Luis O.; Ferreira, Henrique
A simplified curcumin targets the membrane of Bacillus subtilis Journal Article
In: MicrobiologyOpen, vol. 8, iss. 4, pp. e00683, 2019, ISSN: 2045-8827.
Abstract | Links | BibTeX | Tags: antibacterial compound, cell division, membrane permeabilization, turmeric
@article{nokey,
title = {A simplified curcumin targets the membrane of Bacillus subtilis},
author = {Luana G. Morão and Carlos R. Polaquini and Malgorzata Kopacz and Guilherme S. Torrezan and Gabriela M. Ayusso and Guilherme Dilarri and Lúcia B. Cavalca and Aleksandra Zielińska and Dirk Jan Scheffers and Luis O. Regasini and Henrique Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/abs/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/10.1002/mbo3.683},
doi = {10.1002/MBO3.683},
issn = {2045-8827},
year = {2019},
date = {2019-01-01},
journal = {MicrobiologyOpen},
volume = {8},
issue = {4},
pages = {e00683},
publisher = {John Wiley & Sons, Ltd},
abstract = {Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.},
keywords = {antibacterial compound, cell division, membrane permeabilization, turmeric},
pubstate = {published},
tppubtype = {article}
}
Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.
Morão, Luana G.; Polaquini, Carlos R.; Kopacz, Malgorzata; Torrezan, Guilherme S.; Ayusso, Gabriela M.; Dilarri, Guilherme; Cavalca, Lúcia B.; Zielińska, Aleksandra; Scheffers, Dirk Jan; Regasini, Luis O.; Ferreira, Henrique
A simplified curcumin targets the membrane of Bacillus subtilis Journal Article
In: MicrobiologyOpen, vol. 8, iss. 4, pp. e00683, 2019, ISSN: 2045-8827.
Abstract | Links | BibTeX | Tags: antibacterial compound, cell division, membrane permeabilization, turmeric
@article{nokey,
title = {A simplified curcumin targets the membrane of Bacillus subtilis},
author = {Luana G. Morão and Carlos R. Polaquini and Malgorzata Kopacz and Guilherme S. Torrezan and Gabriela M. Ayusso and Guilherme Dilarri and Lúcia B. Cavalca and Aleksandra Zielińska and Dirk Jan Scheffers and Luis O. Regasini and Henrique Ferreira},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/abs/10.1002/mbo3.683 https://onlinelibrary.wiley.com/doi/10.1002/mbo3.683},
doi = {10.1002/MBO3.683},
issn = {2045-8827},
year = {2019},
date = {2019-01-01},
journal = {MicrobiologyOpen},
volume = {8},
issue = {4},
pages = {e00683},
publisher = {John Wiley & Sons, Ltd},
abstract = {Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.},
keywords = {antibacterial compound, cell division, membrane permeabilization, turmeric},
pubstate = {published},
tppubtype = {article}
}
Curcumin is the main constituent of turmeric, a seasoning popularized around the world with Indian cuisine. Among the benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, curcumin inhibits the growth of the gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference with the division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments in a GTP-dependent manner, with the concomitant recruitment of essential factors to operate cell division. By stimulating the GTPase activity of FtsZ, curcumin destabilizes its function. Recently, curcumin was shown to promote membrane permeabilization in B. subtilis. Here, we used molecular simplification to dissect the functionalities of curcumin. A simplified form, in which a monocarbonyl group substituted the β-diketone moiety, showed antibacterial action against gram-positive and gram-negative bacteria of clinical interest. The simplified curcumin also disrupted the divisional septum of B. subtilis; however, subsequent biochemical analysis did not support a direct action on FtsZ. Our results suggest that the simplified curcumin exerted its function mainly through membrane permeabilization, with disruption of the membrane potential necessary for FtsZ intra-cellular localization. Finally, we show here experimental evidence for the requirement of the β-diketone group of curcumin for its interaction with FtsZ.