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2022
Lima, Lídia Passos; Pereira, Juliana Biar; Flores, Anthony Jhoao Fasabi; Lorenzetti, Alan Péricles Rodrigues; Boechat, Ana Laura; Pereda, Maria Claudia; Gualtieri, Sophia; Prado, Daniele Ferreira; Rocha, Diego; Ceseti, Lucas Moraes; Baldini, Regina Lúcia; Farah, Chuck S.; Koide, Tie; Benedetti, Celso Eduardo; Alvarez-Martinez, Cristina E.
An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri Journal Article
In: Journal of Bacteriology, vol. 204, iss. 5, 2022, ISSN: 10985530.
Abstract | Links | BibTeX | Tags: phytopathogens, regulation of gene expression, sigma factors
@article{nokey,
title = {An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri},
author = {Lídia Passos Lima and Juliana Biar Pereira and Anthony Jhoao Fasabi Flores and Alan Péricles Rodrigues Lorenzetti and Ana Laura Boechat and Maria Claudia Pereda and Sophia Gualtieri and Daniele Ferreira Prado and Diego Rocha and Lucas Moraes Ceseti and Regina Lúcia Baldini and Chuck S. Farah and Tie Koide and Celso Eduardo Benedetti and Cristina E. Alvarez-Martinez},
url = {https://journals.asm.org/doi/10.1128/jb.00624-21},
doi = {10.1128/JB.00624-21/SUPPL_FILE/JB.00624-21-S0002.XLSX},
issn = {10985530},
year = {2022},
date = {2022-01-01},
journal = {Journal of Bacteriology},
volume = {204},
issue = {5},
publisher = {American Society for Microbiology},
abstract = {The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.},
keywords = {phytopathogens, regulation of gene expression, sigma factors},
pubstate = {published},
tppubtype = {article}
}
The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.
Lima, Lídia Passos; Pereira, Juliana Biar; Flores, Anthony Jhoao Fasabi; Lorenzetti, Alan Péricles Rodrigues; Boechat, Ana Laura; Pereda, Maria Claudia; Gualtieri, Sophia; Prado, Daniele Ferreira; Rocha, Diego; Ceseti, Lucas Moraes; Baldini, Regina Lúcia; Farah, Chuck S.; Koide, Tie; Benedetti, Celso Eduardo; Alvarez-Martinez, Cristina E.
An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri Journal Article
In: Journal of Bacteriology, vol. 204, iss. 5, 2022, ISSN: 10985530.
Abstract | Links | BibTeX | Tags: phytopathogens, regulation of gene expression, sigma factors
@article{nokey,
title = {An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri},
author = {Lídia Passos Lima and Juliana Biar Pereira and Anthony Jhoao Fasabi Flores and Alan Péricles Rodrigues Lorenzetti and Ana Laura Boechat and Maria Claudia Pereda and Sophia Gualtieri and Daniele Ferreira Prado and Diego Rocha and Lucas Moraes Ceseti and Regina Lúcia Baldini and Chuck S. Farah and Tie Koide and Celso Eduardo Benedetti and Cristina E. Alvarez-Martinez},
url = {https://journals.asm.org/doi/10.1128/jb.00624-21},
doi = {10.1128/JB.00624-21/SUPPL_FILE/JB.00624-21-S0002.XLSX},
issn = {10985530},
year = {2022},
date = {2022-01-01},
journal = {Journal of Bacteriology},
volume = {204},
issue = {5},
publisher = {American Society for Microbiology},
abstract = {The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.},
keywords = {phytopathogens, regulation of gene expression, sigma factors},
pubstate = {published},
tppubtype = {article}
}
The genus Xanthomonas includes more than 30 phytopathogenic species that infect a wide range of plants and cause severe diseases that greatly impact crop productivity. These bacteria are highly adapted to the soil and plant environment, being found in decaying material, as epiphytes, and colonizing the plant mesophyll. Signal transduction mechanisms involved in the responses of Xanthomonas to environmental changes are still poorly characterized. Xanthomonad genomes typically encode several representatives of the extracytoplasmic function s (s ECF) factors, whose physiological roles remain elusive. In this work, we functionally characterized the Xanthomonas citri pv. citri EcfL, a s ECF factor homologous to members of the iron-responsive FecI-like group. We show that EcfL is not required or induced during iron starvation, despite presenting the common features of other FecI-like s ECF factors. EcfL positively regulates one operon composed of three genes that encode a TonB-dependent receptor involved in cell surface signaling, an acid phosphatase, and a lectin-domain containing protein. Furthermore, we demonstrate that EcfL is required for full virulence in citrus, and its regulon is induced inside the plant mesophyll and in response to acid stress. Together, our study suggests a role for EcfL in the adaptation of X. citri to the plant environment, in this way contributing to its ability to cause citrus canker disease.
2021
Bueno, Danilo; Pedrolli, Danielle B.; Martins, Paula M. M.; Bocchini, Daniela A.; Moraes, Karen C. M.; Facincani, Agda P.; Ferro, Jesus A.; Varani, Alessandro M.; Pena, Michelle M.; Ferreira, Henrique
Riboswitch theo/metE as a Transcription Regulation Tool for Xanthomonas citri subsp. citri Journal Article
In: Microorganisms 2021, Vol. 9, Page 329, vol. 9, iss. 2, pp. 329, 2021, ISSN: 2076-2607.
Abstract | Links | BibTeX | Tags: <i>parB</i>, Chromosome segregation, Citrus canker, pNPTS138 sequence, regulation of gene expression
@article{Bueno2021,
title = {Riboswitch theo/metE as a Transcription Regulation Tool for Xanthomonas citri subsp. citri},
author = {Danilo Bueno and Danielle B. Pedrolli and Paula M. M. Martins and Daniela A. Bocchini and Karen C. M. Moraes and Agda P. Facincani and Jesus A. Ferro and Alessandro M. Varani and Michelle M. Pena and Henrique Ferreira},
url = {https://www.mdpi.com/2076-2607/9/2/329/htm https://www.mdpi.com/2076-2607/9/2/329},
doi = {10.3390/MICROORGANISMS9020329},
issn = {2076-2607},
year = {2021},
date = {2021-01-01},
journal = {Microorganisms 2021, Vol. 9, Page 329},
volume = {9},
issue = {2},
pages = {329},
publisher = {Multidisciplinary Digital Publishing Institute},
abstract = {Xanthomonas citri subsp. citri (X. citri) is the causal agent of Asiatic Citrus Canker (ACC), a disease that affects citrus. ACC has no cure, and growers must rely on special agricultural practices to prevent bacterial spreading. Understanding X. citri basic biology is essential to foresee potential genetic targets to control ACC. Traditionally, microbial genetics use gene deletion/disruption to investigate gene function. However, essential genes are difficult to study this way. Techniques based on small-RNAs and antisense-RNAs are powerful for gene characterization, but not yet fully explored in prokaryotes. One alternative is riboswitches, which derive from bacteria, and can control transcription/translation. Riboswitches are non-coding RNAs able to modulate gene expression in the presence of specific ligands. Here we demonstrate that the riboswitch theo/metE decreases parB expression in X. citri in a platform responsive to theophylline. By monitoring cell respiration, we showed that higher concentrations of the ligand interfered with bacterial viability. Therefore, we determined the safe dose of theophylline to be used with X. citri. Finally, in downstream investigations of parB transcription modulation, we show evidence for the fact that ParB is stable, remains functional throughout the cell cycle, and is inherited by the daughter cells upon cell division.},
keywords = {<i>parB</i>, Chromosome segregation, Citrus canker, pNPTS138 sequence, regulation of gene expression},
pubstate = {published},
tppubtype = {article}
}
Xanthomonas citri subsp. citri (X. citri) is the causal agent of Asiatic Citrus Canker (ACC), a disease that affects citrus. ACC has no cure, and growers must rely on special agricultural practices to prevent bacterial spreading. Understanding X. citri basic biology is essential to foresee potential genetic targets to control ACC. Traditionally, microbial genetics use gene deletion/disruption to investigate gene function. However, essential genes are difficult to study this way. Techniques based on small-RNAs and antisense-RNAs are powerful for gene characterization, but not yet fully explored in prokaryotes. One alternative is riboswitches, which derive from bacteria, and can control transcription/translation. Riboswitches are non-coding RNAs able to modulate gene expression in the presence of specific ligands. Here we demonstrate that the riboswitch theo/metE decreases parB expression in X. citri in a platform responsive to theophylline. By monitoring cell respiration, we showed that higher concentrations of the ligand interfered with bacterial viability. Therefore, we determined the safe dose of theophylline to be used with X. citri. Finally, in downstream investigations of parB transcription modulation, we show evidence for the fact that ParB is stable, remains functional throughout the cell cycle, and is inherited by the daughter cells upon cell division.